Emerging role of homo- and heterodimerization in G-protein-coupled receptor biosynthesis and maturation

Two subsets of memory T lymphocytes with distinct homing potentials and effector functions

1999 • 5,659 citations

Crystal Structure of Rhodopsin: A G Protein-Coupled Receptor

2000 • 5,539 citations

Chemokines

2000 • 3,773 citations

Blood Monocytes Consist of Two Principal Subsets with Distinct Migratory Properties

2003 • 3,265 citations

Atherosclerosis

2001 • 2,982 citations

Chemokines and leukocyte traffic

1998 • 2,755 citations

Immunol. Cell Biol.

1995 • 2,564 citations

Endothelial Cells in Physiology and in the Pathophysiology of Vascular Disorders

1998 • 2,263 citations

RAMPs regulate the transport and ligand specificity of the calcitonin-receptor-like receptor

1998 • 2,076 citations

Quality control in the endoplasmic reticulum

2003 • 2,061 citations

Hyperthermophilic Enzymes: Sources, Uses, and Molecular Mechanisms for Thermostability

2001 • 1,993 citations

A new class of membrane-bound chemokine with a CX3C motif

1997 • 1,964 citations

Rapid evolution of a protein in vitro by DNA shuffling

1994 • 1,901 citations

The lineage decisions of helper T cells

2002 • 1,678 citations

Escherichia coli and Salmonella.

1996 • 1,585 citations

Mammalian Sweet Taste Receptors

2001 • 1,550 citations

Molecular tinkering of G protein-coupled receptors: an evolutionary success

1999 • 1,479 citations

An amino-acid taste receptor

2002 • 1,419 citations

Industrial enzyme applications

2002 • 1,407 citations

Identification and Molecular Characterization of Fractalkine Receptor CX3CR1, which Mediates Both Leukocyte Migration and Adhesion

1997 • 1,406 citations

Expanding the Genetic Code of Escherichia coli

2001 • 1,405 citations

Lymphocyte traffic control by chemokines

2001 • 1,225 citations

Structural basis of glutamate recognition by a dimeric metabotropic glutamate receptor

2000 • 1,197 citations

G-protein-coupled receptor heterodimerization modulates receptor function

1999 • 1,146 citations

The 2.0 Å crystal structure of a heterotrimeric G protein

1996 • 1,118 citations

The Role of Chemokine Receptors in Primary, Effector, and Memory Immune Responses

2000 • 1,077 citations

A New ER Trafficking Signal Regulates the Subunit Stoichiometry of Plasma Membrane KATP Channels

1999 • 1,038 citations

Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains

1991 • 1,014 citations

Isolation of high affinity human antibodies directly from large synthetic repertoires.

1994 • 970 citations

Man-made cell-like compartments for molecular evolution

1998 • 928 citations

T-cell adhesion induced by proteoglycan-immobilized cytokine MIP-lβ

1993 • 896 citations

Enzymes for chemical synthesis

2001 • 877 citations

Conformational change of proteins arising from normal mode calculations

2001 • 874 citations

Receptors for Dopamine and Somatostatin: Formation of Hetero-Oligomers with Enhanced Functional Activity

2000 • 866 citations

Immune mechanisms in atherosclerosis.

1989 • 827 citations

DNA shuffling of a family of genes from diverse species accelerates directed evolution

1998 • 821 citations

An Expanded Eukaryotic Genetic Code

2003 • 799 citations

Chemokine receptors and their role in inflammation and infectious diseases

2000 • 793 citations

A Peptide Derived from a β2-Adrenergic Receptor Transmembrane Domain Inhibits Both Receptor Dimerization and Activation

1996 • 758 citations

Molecular evolution by staggered extension process (StEP) in vitro recombination

1998 • 742 citations

Crystal structure of a GA protein βγdimer at 2.1 Å resolution

1996 • 741 citations

Chemokine receptors: multifaceted therapeutic targets

2002 • 738 citations

Rhodopsin dimers in native disc membranes

2003 • 734 citations

Chemokines and the Tissue-Specific Migration of Lymphocytes

2002 • 692 citations

Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli

2002 • 680 citations

Fractalkine and CX3CR1 Mediate a Novel Mechanism of Leukocyte Capture, Firm Adhesion, and Activation under Physiologic Flow

1998 • 679 citations

Roles of G‐protein‐coupled receptor dimerization

2004 • 668 citations

A Trafficking Checkpoint Controls GABAB Receptor Heterodimerization

2000 • 666 citations

Neurotactin, a membrane-anchored chemokine upregulated in brain inflammation

1997 • 656 citations

Oligomerization of G-protein-coupled transmitter receptors

2001 • 652 citations

Evolution, structure, and activation mechanism of family 3/C G-protein-coupled receptors

2003 • 629 citations

Tumor Necrosis Factor-α-converting Enzyme (ADAM17) Mediates the Cleavage and Shedding of Fractalkine (CX3CL1)

2001 • 623 citations

G-Protein-coupled receptor oligomerization and its potential for drug discovery

2002 • 584 citations

Dimerization: An Emerging Concept for G Protein–Coupled Receptor Ontogeny and Function

2002 • 580 citations

Combinatorial and Evolution-Based Methods in the Creation of Enantioselective Catalysts

2001 • 578 citations

Fractalkine Preferentially Mediates Arrest and Migration of CD16+ Monocytes

2003 • 563 citations

Functional proteins from a random-sequence library

2001 • 544 citations

Lipases as practical biocatalysts

2002 • 533 citations

Subtypes of the Somatostatin Receptor Assemble as Functional Homo- and Heterodimers

2000 • 528 citations

Infection With Chlamydia pneumoniae Accelerates the Development of Atherosclerosis and Treatment With Azithromycin Prevents It in a Rabbit Model

1998 • 522 citations

Infection and Atherosclerosis

1999 • 516 citations

AT1-receptor heterodimers show enhanced G-protein activation and altered receptor sequestration

2000 • 487 citations

Increased AT1 receptor heterodimers in preeclampsia mediate enhanced angiotensin II responsiveness

2001 • 485 citations

Dimerization of the δ Opioid Receptor:

1997 • 479 citations

Rules of chemokine receptor association with T cell polarization in vivo

2001 • 472 citations

Quantitative Assessment of β1- and β2-Adrenergic Receptor Homo- and Heterodimerization by Bioluminescence Resonance Energy Transfer

2002 • 466 citations

Decreased Atherosclerotic Lesion Formation in CX3CR1/Apolipoprotein E Double Knockout Mice

2003 • 465 citations

Improved biocatalysts by directed evolution and rational protein design

2001 • 462 citations

Decreased atherosclerosis in CX3CR1–/– mice reveals a role for fractalkine in atherogenesis

2003 • 460 citations

Leukocyte extravasation: chemokine transport and presentation by the endothelium

2002 • 446 citations

Transcriptional regulation of Th1/Th2 polarization

2000 • 433 citations

Chemokines in Immunity

2001 • 431 citations

Measurement of the millisecond activation switch of G protein–coupled receptors in living cells

2003 • 419 citations

How do cytotoxic lymphocytes kill their targets?

1998 • 398 citations

Mechanism of Transdominant Inhibition of CCR5-mediated HIV-1 Infection by ccr5Δ32

1997 • 371 citations

Addition of the keto functional group to the genetic code of Escherichia coli

2002 • 348 citations

Polymorphism in the fractalkine receptor CX3CR1 as a genetic risk factor for coronary artery disease

2001 • 348 citations

GABAB receptors – the first 7TM heterodimers

1999 • 345 citations

Molecular Mechanisms and Therapeutical Implications of Intramembrane Receptor/Receptor Interactions among Heptahelical Receptors with Examples from the Striatopallidal GABA Neurons

2003 • 326 citations

Selection in chemostats

1983 • 323 citations

Dual Functions of Fractalkine/CX3C Ligand 1 in Trafficking of Perforin+/Granzyme B+ Cytotoxic Effector Lymphocytes That Are Defined by CX3CR1 Expression

2002 • 320 citations

Fractalkine (CX3CL1) as an amplification circuit of polarized Th1 responses

2001 • 318 citations

Directed evolution of an extremely fast phosphotriesterase by in vitro compartmentalization

2002 • 314 citations

G-protein-coupled receptors function as oligomers in vivo

2000 • 313 citations

Monitoring of Ligand-independent Dimerization and Ligand-induced Conformational Changes of Melatonin Receptors in Living Cells by Bioluminescence Resonance Energy Transfer

2002 • 311 citations

Exploiting recombination in single bacteria to make large phage antibody libraries

2000 • 311 citations

The cyclophilin homolog NinaA functions as a chaperone, forming a stable complex in vivo with its protein target rhodopsin.

1994 • 305 citations

Oxytocin and Vasopressin V1a and V2 Receptors Form Constitutive Homo- and Heterodimers during Biosynthesis

2003 • 303 citations

Directed enzyme evolution

2001 • 301 citations

C-Terminal Interaction Is Essential for Surface Trafficking But Not for Heteromeric Assembly of GABA_BReceptors

2001 • 299 citations

Functional Expression of Murine V2R Pheromone Receptors Involves Selective Association with the M10 and M1 Families of MHC Class Ib Molecules

2003 • 292 citations

Homodimerization of the β2-Adrenergic Receptor as a Prerequisite for Cell Surface Targeting

2004 • 291 citations

The Fourth Transmembrane Segment Forms the Interface of the Dopamine D2 Receptor Homodimer

2003 • 285 citations

Creation of drug-specific herpes simplex virus type 1 thymidine kinase mutants for gene therapy.

1996 • 278 citations

Leukocyte transendothelial migration: orchestrating the underlying molecular machinery

2001 • 278 citations

Regulation of transport of the dopamine D1 receptor by a new membrane-associated ER protein

2001 • 275 citations

Structure-based Analysis of GPCR Function: Evidence for a Novel Pentameric Assembly between the Dimeric Leukotriene B4 Receptor BLT1 and the G-protein

2003 • 270 citations

CX₃C Chemokine Fractalkine in Pulmonary Arterial Hypertension

2002 • 269 citations

Closed state of both binding domains of homodimeric mGlu receptors is required for full activity

2004 • 267 citations

Proteolytic selection for protein folding using filamentous bacteriophages

1998 • 262 citations

Monitoring Receptor Oligomerization Using Time-resolved Fluorescence Resonance Energy Transfer and Bioluminescence Resonance Energy Transfer

2001 • 257 citations

Association Between Polymorphism in the Chemokine Receptor CX3CR1 and Coronary Vascular Endothelial Dysfunction and Atherosclerosis

2001 • 257 citations

Odorant Receptor Localization to Olfactory Cilia Is Mediated by ODR-4, a Novel Membrane-Associated Protein

1998 • 250 citations

Identification and Molecular Characterization of m3 Muscarinic Receptor Dimers

1999 • 250 citations

Simplified Normal Mode Analysis of Conformational Transitions in DNA-dependent Polymerases: the Elastic Network Model

2002 • 249 citations

In Vivo Inhibition of CC and CX3C Chemokine–induced Leukocyte Infiltration and Attenuation of Glomerulonephritis in Wistar-Kyoto (WKY) Rats by vMIP-II

1998 • 247 citations

Molecular Uncoupling of Fractalkine-mediated Cell Adhesion and Signal Transduction

1999 • 242 citations

Constitutive Signaling of the Human Cytomegalovirus-encoded Chemokine Receptor US28

2001 • 239 citations

Fractalkine, a novel chemokine in rheumatoid arthritis and in rat adjuvant-induced arthritis

2001 • 236 citations

Predicting the emergence of antibiotic resistance by directed evolution and structural analysis.

2001 • 233 citations

Chemokines, Chemokine Receptors, and Allograft Rejection

2001 • 232 citations

The chemokine monocyte chemoattractant protein-1 induces functional responses through dimerization of its receptor CCR2

1999 • 229 citations

CX3C-Chemokine, Fractalkine-Enhanced Adhesion of THP-1 Cells to Endothelial Cells Through Integrin-Dependent and -Independent Mechanisms

2000 • 227 citations

Directed Evolution and Biocatalysis

2001 • 224 citations

Differentiation of Human CD8+ T Cells from a Memory to Memory/Effector Phenotype

2002 • 224 citations

Ligand-independent Dimerization of CXCR4, a Principal HIV-1 Coreceptor

2003 • 222 citations

Tumor Necrosis Factor-α-converting Enzyme Mediates the Inducible Cleavage of Fractalkine

2001 • 221 citations

Constitutive Agonist-independent CCR5 Oligomerization and Antibody-mediated Clustering Occurring at Physiological Levels of Receptors

2002 • 209 citations

14-3-3 Dimers Probe the Assembly Status of Multimeric Membrane Proteins

2003 • 209 citations

Homo- and hetero-oligomeric interactions between G-protein-coupled receptors in living cells monitored by two variants of bioluminescence resonance energy transfer (BRET): hetero-oligomers between receptor subtypes form more efficiently than between less closely related sequences

2002 • 207 citations

The effect of high-frequency random mutagenesis on in vitro protein evolution: a study on TEM-1 β-lactamase 1 1Edited by A. R. Fersht

1999 • 204 citations

Selective recognition of the membrane‐bound CX₃C chemokine, fractalkine, by the human cytomegalovirus‐encoded broad‐spectrum receptor US28

1998 • 203 citations

An efficient system for the evolution of aminoacyl-tRNA synthetase specificity

2002 • 202 citations

Investigating and Engineering Enzymes by Genetic Selection

2001 • 202 citations

Directed evolution of an aspartate aminotransferase with new substrate specificities

1998 • 196 citations

Constitutive and Agonist-dependent Homo-oligomerization of the Thyrotropin-releasing Hormone Receptor

2001 • 194 citations

G Protein–Coupled Receptor Oligomerization

2004 • 193 citations

Preferential Formation of MT₁/MT₂ Melatonin Receptor Heterodimers with Distinct Ligand Interaction Properties Compared with MT₂ Homodimers

2004 • 193 citations

IL-1β and IFN-γ induce the expression of diverse chemokines and IL-15 in human and rat pancreatic islet cells, and in islets from pre-diabetic NOD mice

2003 • 192 citations

Neuronal Fractalkine Expression in HIV-1 Encephalitis: Roles for Macrophage Recruitment and Neuroprotection in the Central Nervous System

2000 • 191 citations

Receptor activity modifying proteins

2001 • 188 citations

Ligand-independent growth hormone receptor dimerization occurs in the endoplasmic reticulum and is required for ubiquitin system-dependent endocytosis

2002 • 188 citations

Prevention of crescentic glomerulonephritis by immunoneutralization of the fractalkine receptor CX3CR1: Rapid Communication

1999 • 187 citations

Faces and phases of human CD8+ T-cell development

1999 • 187 citations

Ligand binding to somatostatin receptors induces receptor-specific oligomer formation in live cells

2002 • 186 citations

Identification of amino acid residues crucial for chemokine receptor dimerization

2004 • 184 citations

Identification of the Cysteine Residues in the Amino-terminal Extracellular Domain of the Human Ca2+ Receptor Critical for Dimerization

1999 • 184 citations

Linked Chromosome 16q13 Chemokines, Macrophage-Derived Chemokine, Fractalkine, and Thymus- and Activation-Regulated Chemokine, Are Expressed in Human Atherosclerotic Lesions

2001 • 182 citations

Metabotropic Glutamate 1α and Adenosine A1 Receptors Assemble into Functionally Interacting Complexes

2001 • 179 citations

Random-priming in vitro recombination: An effective tool for directed evolution

1998 • 179 citations

Enlarging the Amino Acid Set of Escherichia coli by Infiltration of the Valine Coding Pathway

2001 • 179 citations

Fractalkine: A Novel Angiogenic Chemokine in Rheumatoid Arthritis

2001 • 176 citations

Directed evolution of enzymes and pathways for industrial biocatalysis

2002 • 174 citations

Evolutionary Potential of (β/α)₈-Barrels: Functional Promiscuity Produced by Single Substitutions in the Enolase Superfamily

2003 • 173 citations

Directed evolution of a (βα) ₈ -barrel enzyme to catalyze related reactions in two different metabolic pathways

2000 • 172 citations

Mutant Frizzled 4 associated with vitreoretinopathy traps wild-type Frizzled in the endoplasmic reticulum by oligomerization

2003 • 169 citations

Making artificial antibodies: A format for phage display of combinatorial heterodimeric arrays

1999 • 169 citations

A Role for Fractalkine and Its Receptor (CX3CR1) in Cardiac Allograft Rejection

2000 • 167 citations

Targeted deletion of CX3CR1 reveals a role for fractalkine in cardiac allograft rejection

2001 • 167 citations

Dimerization of G-protein-coupled receptors: roles in signal transduction

2003 • 166 citations

High-throughput screening for biocatalysts

2001 • 166 citations

Directed evolution and the creation of enantioselective biocatalysts

2001 • 165 citations

Directed Evolution of Thermostable Kanamycin-Resistance Gene: A Convenient Selection Marker for Thermus thermophilus

1999 • 164 citations

Substrate specificity of trypsin investigated by using a genetic selection.

1990 • 160 citations

Targeted gene evolution in Escherichia coli using a highly error-prone DNA polymerase I

2003 • 157 citations

Cell Surface Expression of α1D-Adrenergic Receptors Is Controlled by Heterodimerization with α1B-Adrenergic Receptors

2004 • 156 citations

De novo design of biocatalysts

2002 • 155 citations

Migration of CX3CR1‐positive T cells producing type 1 cytokines and cytotoxic molecules into the synovium of patients with rheumatoid arthritis

2002 • 155 citations

Exploring the active site of chorismate mutase by combinatorial mutagenesis and selection: the importance of electrostatic catalysis.

1996 • 153 citations

Characterization of fractalkine (CX3CL1) and CX3CR1 in human coronary arteries with native atherosclerosis, diabetes mellitus, and transplant vascular disease

2002 • 151 citations

Gene duplication in experimental enzyme evolution

1974 • 151 citations

Hetero-oligomerization between β2- and β3-Adrenergic Receptors Generates a β-Adrenergic Signaling Unit with Distinct Functional Properties

2004 • 151 citations

Directed evolution of novel polymerase activities: Mutation of a DNA polymerase into an efficient RNA polymerase

2002 • 150 citations

Methods to increase enantioselectivity of lipases and esterases

2002 • 145 citations

Directed Evolution Library Creation

2003 • 143 citations

Screening and Selection Methods for Large-Scale Analysis of Protein Function

2002 • 143 citations

Oligomeric Structure of Type I and Type II Transforming Growth Factor β Receptors: Homodimers Form in the ER and Persist at the Plasma Membrane

1998 • 139 citations

A method for directed evolution and functional cloning of enzymes

1998 • 139 citations

HIV-1 infection through the CCR5 receptor is blocked by receptor dimerization

2000 • 138 citations

Fractalkine and vascular injury

2001 • 136 citations

Membrane Trafficking of Heterotrimeric G Proteins via the Endoplasmic Reticulum and Golgi

2002 • 136 citations

The Extracellular Calcium-sensing Receptor Dimerizes through Multiple Types of Intermolecular Interactions

2001 • 136 citations

Dimerization of G-Protein-Coupled Receptors

2001 • 136 citations

Oligomerization, Biogenesis, and Signaling Is Promoted by a Glycophorin A-like Dimerization Motif in Transmembrane Domain 1 of a Yeast G Protein-coupled Receptor

2003 • 136 citations

Tailoring new enzyme functions by rational redesign

2000 • 135 citations

Redesigning Enzyme Topology by Directed Evolution

1998 • 134 citations

An efficient method for generating proteins with altered enzymatic properties: application to beta-lactamase.

1989 • 134 citations

D2 Dopamine Receptor Homodimerization Is Mediated by Multiple Sites of Interaction, Including an Intermolecular Interaction Involving Transmembrane Domain 4

2003 • 131 citations

Cytokine fields and the polarization of the immune response

2001 • 131 citations

Natural killer cells and natural killer T cells

2000 • 130 citations

Man-made enzymes — from design to in vitro compartmentalisation

2000 • 130 citations

Association of GABAB Receptors and Members of the 14-3-3 Family of Signaling Proteins

2001 • 127 citations

Fractalkine expression in human renal inflammation

2001 • 124 citations

Selection for improved subtiligases by phage display

1999 • 122 citations

Involvement of the Amino Terminus of the B2 Receptor in Agonist-induced Receptor Dimerization

1999 • 119 citations

Conversion of Product Specificity of Archaebacterial Geranylgeranyl-diphosphate Synthase

1996 • 115 citations

Inflammation and immune responses in atherosclerosis

2002 • 115 citations

Applications of novel resonance energy transfer techniques to study dynamic hormone receptor interactions in living cells

2002 • 114 citations

Overview of screening for new microbial catalysts and their uses in organic synthesis—selection and optimization of biocatalysts

2002 • 114 citations

Human Immunodeficiency Virus Reverse Transcriptase

1996 • 112 citations

Beyond binding: using phage display to select for structure, folding and enzymatic activity in proteins

1999 • 112 citations

Constructing high complexity synthetic libraries of long ORFs using In Vitro selection

2000 • 112 citations

Olfactory receptor surface expression is driven by association with the β₂-adrenergic receptor

2004 • 108 citations

Assembly, sorting, and exit of oligomeric proteins from the endoplasmic reticulum

1998 • 108 citations

Up-regulated expression of fractalkine and its receptor CX3CR1 during liver injury in humans

2002 • 106 citations

Folding of Insulin Receptor Monomers Is Facilitated by the Molecular Chaperones Calnexin and Calreticulin and Impaired by Rapid Dimerization

1998 • 105 citations

A strategy for the isolation of catalytic activities from repertoires of enzymes displayed on phage 1 1Edited by J. Karn

1999 • 105 citations

The Chaperone Environment at the Cytoplasmic Face of the Endoplasmic Reticulum Can Modulate Rhodopsin Processing and Inclusion Formation

2003 • 104 citations

Characterization of Herpes Simplex Virus type 1 thymidine kinase mutants engineered for improved ganciclovir or acyclovir activity

2002 • 102 citations

The role of fractalkine in the recruitment of monocytes to the endothelium

2000 • 102 citations

Multiple Interactions between Transmembrane Helices Generate the Oligomeric α_1b-Adrenoceptor

2004 • 100 citations

Heterotrimer Formation, Together with Isoprenylation, Is Required for Plasma Membrane Targeting of Gऔγ

2003 • 98 citations

Expression of the fractalkine receptor (CX3CR1) in human kidney diseases

2002 • 96 citations

Fractalkine, a CX₃C‐chemokine, functions predominantly as an adhesion molecule in monocytic cell line THP‐1

2001 • 94 citations

Olfactory Receptor Trafficking Involves Conserved Regulatory Steps

2001 • 93 citations

Systematic mutagenesis of the active site omega loop of TEM-1 beta-lactamase

1996 • 92 citations

The ζ Isoform of 14-3-3 Proteins Interacts with the Third Intracellular Loop of Different α2-Adrenergic Receptor Subtypes

1999 • 92 citations

Cytomegalovirus-Mediated Upregulation of Chemokine Expression Correlates with the Acceleration of Chronic Rejection in Rat Heart Transplants

2003 • 88 citations

G-protein coupled receptor oligomerization in neuroendocrine pathways

2003 • 88 citations

Prediction of heterodimerization interfaces of G-protein coupled receptors with a new subtractive correlated mutation method

2002 • 88 citations

Directed evolution of an esterase: screening of enzyme libraries based on ph-Indicators and a growth assay

1999 • 87 citations

A Method for the Selection of Catalytic Activity Using Phage Display and Proximity Coupling

1999 • 85 citations

Potassium Channels

1999 • 83 citations

Probing intermolecular protein-protein interactions in the calcium-sensing receptor homodimer using bioluminescence resonance energy transfer (BRET)

2002 • 82 citations

In Vivo Differentiated Cytokine-Producing CD4+ T Cells Express Functional CCR7

2002 • 82 citations

Directed Evolution To Investigate Steric Control of Enzymatic Oxidosqualene Cyclization. An Isoleucine-to-Valine Mutation in Cycloartenol Synthase Allows Lanosterol and Parkeol Biosynthesis

1999 • 82 citations

Random mutagenesis of Thermus aquaticus DNA polymerase I: concordance of immutable sites in vivo with the crystal structure.

1996 • 80 citations

Improving enzymes for cancer gene therapy

1999 • 79 citations

Serial increase in the thermal stability of 3‐isopropylmalate dehydrogenase from Bacillus subtilis by experimental evolution

1998 • 78 citations

Mimicking natural evolution in vitro : An N -acetylneuraminate lyase mutant with an increased dihydrodipicolinate synthase activity

2003 • 77 citations

Low Fidelity Mutants in the O-Helix of Thermus aquaticus DNA Polymerase I

1997 • 76 citations

Towards novel processes for the fine-chemical and pharmaceutical industries

2002 • 70 citations

Evolution in Action

1974 • 70 citations

Cellular localization of fractalkine at sites of inflammation: antigen-presenting cells in psoriasis express high levels of fractalkine

2001 • 69 citations

Selection and Characterization of Amino Acid Substitutions at Residues 237-240 of TEM-1 β-Lactamase with Altered Substrate Specificity for Aztreonam and Ceftazidime

1996 • 69 citations

Mutants generated by the insertion of random oligonucleotides into the active site of the .beta.-lactamase gene

1989 • 68 citations

Naturally Occurring Deletional Mutation in the C-Terminal Cytoplasmic Tail of CCR5 Affects Surface Trafficking of CCR5

2001 • 68 citations

Searching sequence space for protein catalysts

2001 • 68 citations

Fractalkine expression on human renal tubular epithelial cells: potential role in mononuclear cell adhesion

2002 • 63 citations

Interferon- γ Stimulates Fractalkine Expression in Human Bronchial Epithelial Cells and Regulates Mononuclear Cell Adherence

2001 • 63 citations

Further stabilization of 3-isopropylmalate dehydrogenase of an extreme thermophile, Thermus thermophilus, by a suppressor mutation method

1996 • 62 citations

Altering the Substrate Specificity of Cephalosporin Acylase by Directed Evolution of the β-Subunit

2002 • 62 citations

Binding of Agonist but Not Antagonist Leads to Fluorescence Resonance Energy Transfer between Intrinsically Fluorescent Gonadotropin-Releasing Hormone Receptors

2001 • 61 citations

Investigating the target recognition of DNA cytosine-5 methyltransferase HhaI by library selection using in vitro compartmentalisation

2002 • 60 citations

Discovery of Superior Enzymes by Directed Molecular Evolution

2001 • 60 citations

An Error-Prone T7 RNA Polymerase Mutant Generated by Directed Evolution

2001 • 58 citations

Using an AraC-based three-hybrid system to detect biocatalysts in vivo

2000 • 58 citations

Upregulation of Fractalkine in Human Crescentic Glomerulonephritis

2001 • 58 citations

A strategy of exon shuffling for making large peptide repertoires displayed on filamentous bacteriophage.

1996 • 58 citations

Chemostat Approach for the Directed Evolution of Biodesulfurization Gain-of-Function Mutants

2002 • 58 citations

Mutations in human DNA polymerase eta motif II alter bypass of DNA lesions

2001 • 55 citations

Adenovirus-mediated gene transfer of enhanced Herpes simplex virus thymidine kinase mutants improves prodrug-mediated tumor cell killing

2003 • 54 citations

Functional Cloning, Heterologous Expression, and Purification of Two Different N-Deoxyribosyltransferases fromLactobacillus helveticus

2002 • 52 citations

Fractalkine expression and the recruitment of CX3CR1+ cells in the prolonged mesangial proliferative glomerulonephritis

2002 • 52 citations

Lipid-facing correlated mutations and dimerization in G-protein coupled receptors

2001 • 52 citations

A genetic system to identify DNA polymerase β mutator mutants

1997 • 50 citations

Directed evolution of a glutaryl acylase into an adipyl acylase

2002 • 50 citations

Evolutionary optimisation of enzymes

2000 • 48 citations

Novel human DNA alkyltransferases obtained by random substitution and genetic selection in bacteria.

1996 • 45 citations

Improving the display of proteins on filamentous phage

2001 • 45 citations

Distribution of Mutations in Human Thymidylate Synthase Yielding Resistance to 5-Fluorodeoxyuridine

2002 • 44 citations

Adaptation of a thermophilic enzyme, 3-isopropylmalate dehydrogenase, to low temperatures

2001 • 42 citations

Human fractalkine mediates leukocyte adhesion but not capture under physiological shear conditions; a mechanism for selective monocyte recruitment

2003 • 41 citations

Creating novel enzymes by applied molecular evolution

1997 • 41 citations

Random Sequence Mutagenesis and Resistance to 5-Fluorouridine in Human Thymidylate Synthases

1998 • 39 citations

In Vivo Mutagenesis by Escherichia coliDNA Polymerase I

2001 • 39 citations

Directed Molecular Evolution of Proteins or How to Improve Enzymes for Biocatalysis

2002 • 38 citations

Is ‘timing’ important for cytokine polarization?

2002 • 36 citations

Phage display selects for amylases with improved low pH starch-binding

2002 • 36 citations

Thymidine kinase mutants obtained by random sequence selection.

1993 • 35 citations

Human keratinocytes express fractalkine/CX3CL1

2003 • 35 citations

Selection of amidases with novel substrate specificities from penicillin amidase of Escherichia coli

1989 • 34 citations

Engineering human DNA alkyltransferases for gene therapy using random sequence mutagenesis.

1998 • 34 citations

Enzyme evolution in Rhodobacter sphaeroides: selection of a mutant expressing a new galactitol dehydrogenase and biochemical characterization of the enzyme

1995 • 34 citations

Experimental Evolution of Enzyme Temperature Activity Profile: Selection In Vivo and Characterization of Low-Temperature-Adapted Mutants of Pyrococcus furiosus Ornithine Carbamoyltransferase

2001 • 33 citations

Increased CD8 ⁺ T Cells Associated With Chlamydia pneumoniae in Symptomatic Carotid Plaque

2001 • 33 citations

A New TEM β -Lactamase Double Mutant with Broadened Specificity Reveals Substrate-dependent Functional Interactions

1995 • 33 citations

Selection of Metalloenzymes by Catalytic Activity Using Phage Display and Catalytic Elution

2001 • 32 citations

Selection of β-lactamases and penicillin binding mutants from a library of phage displayed TEM-1 β-lactamase randomly mutated in the active site Ω-loop 1 1Edited by A. R. Fersht

2000 • 32 citations

In vivo versus in vitro screening or selection for catalytic activity in enzymes and abzymes

1997 • 32 citations

3′-Azido-3′-deoxythymidine-resistant Mutants of DNA Polymerase β Identified by in Vivo Selection

1999 • 30 citations

Novel concepts for selection of catalytic activity

2001 • 30 citations

HIV-1 infection through the CCR5 receptor is blocked by receptor dimerization

2000 • 30 citations

Role of fractalkine in leukocyte adhesion and migration and in vascular injury

2001 • 29 citations

Exocytic pathway‐independent plasma membrane targeting of heterotrimeric G proteins

2004 • 29 citations

Changing the substrate specificity of penicillin G acylase from Kluyvera citrophila through selective pressure

1994 • 29 citations

Thermoadaptation of α-Galactosidase AgaB1 in Thermus thermophilus

2002 • 27 citations

Selection of catalytically active biotin ligase and trypsin mutants by phage display

2001 • 27 citations

Interaction of the parathyroid hormone receptor with the 14-3-3 protein

2003 • 25 citations

Alteration of the catalytic efficiency of penicillin amidase from Escherichia coli

1989 • 25 citations

Binding of Agonist but Not Antagonist Leads to Fluorescence Resonance Energy Transfer between Intrinsically Fluorescent Gonadotropin-Releasing Hormone Receptors

2001 • 25 citations

Two polymorphisms in the fracalkine receptor CX3CR1 are not associated with peripheral arterial disease

2003 • 25 citations

A DNA Polymerase β Mutator Mutant with Reduced Nucleotide Discrimination and Increased Protein Stability^,

2001 • 25 citations

Homology-independent protein engineering

2000 • 24 citations

Rapid in vivo evolution of a β-lactamase using phagemids

2000 • 23 citations

Directed evolution of β‐galactosidase from Escherichia coli by mutator strains defective in the 3′→5′ exonuclease activity of DNA polymerase III

2001 • 22 citations

Chain termination codons and polymerase‐induced frameshift mutations

1997 • 21 citations

Applied molecular evolution of O ⁶ -benzylguanine-resistant DNA alkyltransferases in human hematopoietic cells

2001 • 20 citations

Phage-display evolution of tyrosine kinases with altered nucleotide specificity

2001 • 20 citations

In Vitro Selection for Enzymatic Activity: A Model Study Using Adenylate Cyclase

2003 • 15 citations

Evolutionary Biotechnology — Reflections and Perspectives

1999 • 15 citations

Another Jekyll and Hyde story?

1996 • 14 citations

Combinatorial Chemistry in Biology

1999 • 14 citations

Tolerance of 5-fluorodeoxyuridine resistant human thymidylate synthases to alterations in active site residues

1999 • 14 citations

A rapid and effective procedure for screening protease mutants

1996 • 13 citations

Efficient Display of Two Enzymes on Filamentous Phage Using an Improved Signal Sequence

2003 • 11 citations

The Amino Acid Sequence of Ribitol Dehydrogenase-F, a Mutant Enzyme with Improved Xylitol Dehydrogenase Activity

1999 • 10 citations

Optimisation d’une sélection in vitro d’enzymes

2003 • 8 citations

Proreceptor Dimerization Is Required for Insulin Receptor Post-translational Processing

2004 • 7 citations

Iterative Cycles ofIn VitroProtein Selection for DNA Polymerase Activity

2003 • 7 citations

Substrate turnover and inhibitor binding as selection parameters in directed evolution of blood coagulation factor X_a

2003 • 3 citations

Deleted Work

1955 • 0 citations