De novo design of biocatalysts

Industrial biocatalysis today and tomorrow

2001 • 2,339 citations

A Proficient Enzyme

1995 • 859 citations

High-Resolution Protein Design with Backbone Freedom

1998 • 457 citations

Efficient Aldolase Catalytic Antibodies That Use the Enamine Mechanism of Natural Enzymes

1995 • 451 citations

Combinatorial and computational challenges for biocatalyst design

2001 • 417 citations

Enzyme-like proteins by computational design

2001 • 404 citations

Molecular breeding of carotenoid biosynthetic pathways

2000 • 340 citations

Inverting enantioselectivity by directed evolution of hydantoinase for improved production of l-methionine

2000 • 331 citations

Critical Analysis of Antibody Catalysis

2000 • 260 citations

Construction of new ligand binding sites in proteins of known structure

1991 • 233 citations

Computer-based redesign of a protein folding pathway.

2001 • 223 citations

Exploring Nonnatural Evolutionary Pathways by Saturation Mutagenesis: Rapid Improvement of Protein Function

1999 • 216 citations

Energy functions for protein design

1999 • 214 citations

Directed evolution of an aspartate aminotransferase with new substrate specificities

1998 • 196 citations

A structural view of evolutionary divergence

1999 • 193 citations

Computational protein design

1999 • 193 citations

Directed evolution of new catalytic activity using the α/β-barrel scaffold

2000 • 191 citations

Computational method to reduce the search space for directed protein evolution

2001 • 179 citations

Function-based isolation of novel enzymes from a large library

2000 • 175 citations

Redesigning the Substrate Specificity of an Enzyme by Cumulative Effects of the Mutations of Non-active Site Residues

1999 • 175 citations

Novel methods for biocatalyst screening

2001 • 172 citations

Designed evolution of enzymatic properties

2000 • 162 citations

Side-chain and backbone flexibility in protein core design 1 1Edited by A. R. Fersht

1999 • 146 citations

A method for directed evolution and functional cloning of enzymes

1998 • 139 citations

Tailoring new enzyme functions by rational redesign

2000 • 135 citations

Rational evolutionary design: The theory of in vitro protein evolution

2001 • 135 citations

Computational design of an integrin I domain stabilized in the open high affinity conformation.

2000 • 131 citations

Statistical theory for protein combinatorial libraries. packing interactions, backbone flexibility, and the sequence variability of a main-chain structure11Edited by J. Thornton

2001 • 126 citations

Selection for improved subtiligases by phage display

1999 • 122 citations

Flow cytometric screening of cell-based libraries

2000 • 116 citations

Engineering new functions and altering existing functions

1996 • 116 citations

Combinatorial protein design: strategies for screening protein libraries

1997 • 115 citations

Transition-state stabilization as a measure of the efficiency of antibody catalysis

1995 • 114 citations

Analysis of a local fitness landscape with a model of the rough Mt. Fuji-type landscape: Application to prolyl endopeptidase and thermolysin

2000 • 112 citations

Rational design of nascent metalloenzymes

2000 • 112 citations

Multiple Amino Acid Substitutions Allow DNA Polymerases to Synthesize RNA

2000 • 109 citations

A strategy for the isolation of catalytic activities from repertoires of enzymes displayed on phage 1 1Edited by J. Karn

1999 • 105 citations

Coupling backbone flexibility and amino acid sequence selection in protein design

1997 • 91 citations

A Method for the Selection of Catalytic Activity Using Phage Display and Proximity Coupling

1999 • 85 citations

Computer search algorithms in protein modification and design

1998 • 84 citations

Interchange of Catalytic Activity within the 2-Enoyl-Coenzyme A Hydratase/Isomerase Superfamily Based on a Common Active Site Template

1999 • 69 citations

Engineering cyclophilin into a proline-specific endopeptidase

1998 • 69 citations

Using an AraC-based three-hybrid system to detect biocatalysts in vivo

2000 • 58 citations

The development of new biotechnologies using metalloprotein design

1998 • 55 citations

Fitness Spectrum Among Random Mutants on Mt. Fuji-Type Fitness Landscape

1996 • 41 citations

Nonadditivity of mutational effects on the properties of catalase I and its application to efficient directed evolution

1998 • 37 citations

DuPont, UNC R&D effort yields results

1999 • 31 citations

BIOCATALYSIS GROWS FOR DRUG SYNTHESIS

1999 • 25 citations

Creation of a ribonuclease abzyme through site-directed mutagenesis

1998 • 22 citations

Enzymatic properties of rat DNA polymerase beta mutants obtained by randomized mutagenesis

2001 • 15 citations

Theory of Evolutionary Molecular Engineering through Simultaneous Accumulation of Advantageous Mutations

2000 • 13 citations