DNA shuffling of a family of genes from diverse species accelerates directed evolution

The Origins of Order

1993 • 5,064 citations

The origins of order

1993 • 2,842 citations

Rapid evolution of a protein in vitro by DNA shuffling

1994 • 1,901 citations

DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution.

1994 • 1,274 citations

Improved Green Fluorescent Protein by Molecular Evolution Using DNA Shuffling

1996 • 1,212 citations

Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides

1995 • 715 citations

Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents

1996 • 419 citations

Directed evolution of a fucosidase from a galactosidase by DNA shuffling and screening

1997 • 275 citations

Optimization of DNA shuffling for high fidelity recombination

1997 • 221 citations

Evolution of an enzyme activity: crystallographic structure at 2-A resolution of cephalosporinase from the ampC gene of Enterobacter cloacae P99 and comparison with a class A penicillinase.

1993 • 194 citations

Molecular evolution of an arsenate detoxification pathway by DNA shuffling

1997 • 182 citations

Steps towards life: a perspective on evolution

1993 • 170 citations

Sequence and comparative analysis of three Enterobacter cloacae ampC β-lactamase genes and their products

1988 • 133 citations

Gene sequence and biochemical characterization of FOX-1 from Klebsiella pneumoniae, a new AmpC-type plasmid-mediated beta-lactamase with two molecular variants

1994 • 111 citations

Sequence of the Citrobacter freundii OS60 chromosomal ampC beta-lactamase gene

1986 • 89 citations

Searching Sequence Space

1995 • 65 citations

Nucleotide sequence of the ampC-ampR region from the chromosome of Yersinia enterocolitica

1992 • 49 citations