Selection of catalytically active biotin ligase and trypsin mutants by phage display

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Use of Peptide Libraries to Map the Substrate Specificity of a Peptide-Modifying Enzyme: A 13 Residue Consensus Peptide Specifies Biotinylation in Escherichia coli

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1992 • 161 citations

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1999 • 144 citations

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1998 • 139 citations

Selection for improved subtiligases by phage display

1999 • 122 citations

A strategy for the isolation of catalytic activities from repertoires of enzymes displayed on phage 1 1Edited by J. Karn

1999 • 105 citations

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1995 • 101 citations

Trypsin display on the surface of bacteriophage

1993 • 93 citations

A Method for the Selection of Catalytic Activity Using Phage Display and Proximity Coupling

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Perturbing the polar environment of Asp102 in trypsin: consequences of replacing conserved Ser214

1992 • 77 citations

Quenching of Tryptophan Fluorescence by the Active-Site Disulfide Bridge in the DsbA Protein from Escherichia coli

1997 • 67 citations

Kinetics of Biotinyl-5'-adenylate Synthesis Catalyzed by the Escherichia coli Repressor of Biotin Biosynthesis and the Stability of the Enzyme-Product Complex

1994 • 66 citations

The use of biosensor technology for the engineering of antibodies and enzymes

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Alanine Substitutions in Calmodulin-binding Peptides Result in Unexpected Affinity Enhancement

1996 • 62 citations

Radioactive labeling of recombinant antibody fragments by phosphorylation using human casein kinase II and [γ-32P]-ATP

1996 • 46 citations

In Vitro Enzymatic Biotinylation of Recombinant Fab Fragments through a Peptide Acceptor Tail

1998 • 42 citations

Phage display of enzymes and in vitro selection for catalytic activity

1994 • 40 citations

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Trypsin specificity increased through substrate-assisted catalysis

1995 • 38 citations

Monoclonal antibody against pIII of filamentous phage: an immunological tool to study pIII fusion protein expression in phage display systems

1995 • 38 citations

Antibody-Based Targeting of Angiogenesis

2001 • 33 citations

Selection of β-lactamases and penicillin binding mutants from a library of phage displayed TEM-1 β-lactamase randomly mutated in the active site Ω-loop 1 1Edited by A. R. Fersht

2000 • 32 citations

Effect of Exceptional Valine Replacement for Highly Conserved Alanine-55 on the Catalytic Site Structure of Chymotrypsin-Like Serine Protease.

1998 • 4 citations