Improving enzymes for cancer gene therapy

Bone marrow transplant

1997 • 3,583 citations

Rapid evolution of a protein in vitro by DNA shuffling

1994 • 1,901 citations

In Vivo Gene Transfer with Retroviral Vector-Producer Cells for Treatment of Experimental Brain Tumors

1992 • 1,530 citations

Mutator phenotype may be required for multistage carcinogenesis.

1991 • 1,327 citations

Human Cytochrome P450 Enzymes

1995 • 731 citations

Cancer Chemotherapy: Principles and Practice

1990 • 568 citations

Structure, Function, and Inhibition of O6-Alkylguanine-DNA Alkyltransferase

1995 • 502 citations

In VitroEvidence That Metabolic Cooperation Is Responsible for the Bystander Effect Observed with HSVtkRetroviral Gene Therapy

1993 • 425 citations

The role of human cytochrome P450 enzymes in the metabolism of anticancer agents: implications for drug interactions.

1995 • 334 citations

Mutagenic PCR.

1994 • 298 citations

Creation of drug-specific herpes simplex virus type 1 thymidine kinase mutants for gene therapy.

1996 • 278 citations

Drug sensitivity ("suicide") genes for selective cancer chemotherapy.

1994 • 277 citations

Properties of an altered dihydrofolate reductase encoded by amplified genes in cultured mouse fibroblasts.

1981 • 212 citations

Lymphoma Regression Induced by Ganciclovir in Mice Bearing a Herpes Thymidine Kinase Transgene

1990 • 208 citations

Targeting gene therapy to cancer: a review.

1997 • 186 citations

Protection of bone marrow transplant recipients from lethal doses of methotrexate by the generation of methotrexate-resistant bone marrow.

1987 • 144 citations

Cloning and expression in Escherichia coli of a human cDNA encoding the DNA repair protein N-methylpurine-DNA glycosylase

1991 • 142 citations

Selection for G156A O6-methylguanine DNA methyltransferase gene-transduced hematopoietic progenitors and protection from lethality in mice treated with O6-benzylguanine and 1,3-bis(2-chloroethyl)-1-nitrosourea.

1997 • 115 citations

Increasing DNA repair methyltransferase levels via bone marrow stem cell transduction rescues mice from the toxic effects of 1,3-bis(2-chloroethyl)-1-nitrosourea, a chemotherapeutic alkylating agent.

1996 • 113 citations

A gene transfer strategy for making bone marrow cells resistant to trimetrexate

1996 • 106 citations

The role of the bystander effect in suicide gene therapy

1997 • 105 citations

Amplification of a Polymorphic Dihydrofolate Reductase Gene Expressing an Enzyme with Decreased Binding to Methotrexate in a Human Colon Carcinoma Cell Line, HCT-8R4, Resistant to This Drug

1989 • 101 citations

Chemistry and biochemistry of folates

1984 • 100 citations

Isolation of human O6-alkylguanine-DNA alkyltransferase mutants highly resistant to inactivation by O6-benzylguanine.

1998 • 94 citations

Regulation of thymidylate synthetase in mouse leukemia cells (L1210).

1980 • 94 citations

Promoters selected from random DNA sequences.

1986 • 94 citations

Chemotherapy and chemosensitization of transgenic mice which express the human multidrug resistance gene in bone marrow: efficacy, potency, and toxicity.

1991 • 87 citations

Site-directed mutagenesis of mouse dihydrofolate reductase. Mutants with increased resistance to methotrexate and trimethoprim.

1988 • 86 citations

Functional and nonfunctional mutations distinguished by random recombination of homologous genes

1997 • 72 citations

Interaction of Anticancer Drugs with Hepatic Monooxygenase Enzymes

1989 • 62 citations

Identification of important residues within the putative nucleoside binding site of HSV-1 thymidine kinase by random sequence selection: Analysis of selected mutants in vitro

1993 • 62 citations

Long-term protection of recipient mice from lethal doses of methotrexate by marrow infected with a double-copy vector retrovirus containing a mutant dihydrofolate reductase.

1994 • 62 citations

Active site-directed double mutants of dihydrofolate reductase.

1996 • 61 citations

Forced evolution of glutathione S-transferase to create a more efficient drug detoxication enzyme.

1995 • 59 citations

Isolation and characterization of a variant dihydrofolate reductase cDNA from methotrexate-resistant murine L5178Y cells

1990 • 54 citations

Thymidylate synthase: A target for anticancer drug design

1989 • 52 citations

Detection and characterization of mammalian DNA polymerase beta mutants by functional complementation in Escherichia coli.

1993 • 50 citations

The chemotherapeutic exploitation of virus-specified enzymes

1980 • 49 citations

Chemoprotective gene transfer II: multilineage in vivo protection of haemopoiesis against the effects of an antitumour agent by expression of a mutant human O6-alkylguanine-DNA alkyltransferase

1998 • 47 citations

Post-Transplant Methotrexate Administration Leads to Improved Curability of Mice Bearing a Mammary Tumor Transplanted with Marrow Transduced with a Mutant Human Dihydrofolate Reductase cDNA

1997 • 46 citations

Novel human DNA alkyltransferases obtained by random substitution and genetic selection in bacteria.

1996 • 45 citations

Isolation and Characterization of Thymitaq (AG337) and 5-Fluoro-2-deoxyuridylate-resistant Mutants of Human Thymidylate Synthase from Ethyl Methanesulfonate-exposed Human Sarcoma HT1080 Cells

1998 • 43 citations

Creating novel enzymes by applied molecular evolution

1997 • 41 citations

Random Sequence Mutagenesis and Resistance to 5-Fluorouridine in Human Thymidylate Synthases

1998 • 39 citations

Chemoprotection of normal tissues by transfer of drug resistance genes

1996 • 36 citations

Probing the Folate-binding Site of Human Thymidylate Synthase by Site-directed Mutagenesis

1998 • 34 citations

Engineering human DNA alkyltransferases for gene therapy using random sequence mutagenesis.

1998 • 34 citations

Gene therapy utilizing drug resistance genes: A review

1994 • 33 citations

Creation of human alkyltransferases resistant to O6-benzylguanine.

1997 • 32 citations

Single amino acid substitution defines a naturally occurring genetic variant of human thymidylate synthase.

1990 • 32 citations

Transgenic mice expressing the tyr22 variant of murine DHFR: protection of transgenic marrow transplant recipients from lethal doses of methotrexate.

1997 • 30 citations

Drug-resistant dihydrofolate reductases: generation, expression and therapeutic application.

1996 • 21 citations

Another Jekyll and Hyde story?

1996 • 14 citations

Co-expression of the herpes simplex virus thymidine kinase gene potentiates methotrexate resistance conferred by transfer of a mutated dihydrofolate reductase gene

1997 • 12 citations

Purine salvage rescue by xanthine-guanine phosphoribosyltransferase (XGPRT) potentiates methotrexate resistance conferred by transfer of a mutated dihydrofolate reductase gene.

1998 • 5 citations

Gene Transfer and the Treatment of Childhood Cancer

1998 • 2 citations