Further stabilization of 3-isopropylmalate dehydrogenase of an extreme thermophile, Thermus thermophilus, by a suppressor mutation method

Enhanced protein thermostability from site-directed mutations that decrease the entropy of unfolding.

1987 • 738 citations

Description of Thermus thermophilus (Yoshida and Oshima) comb. nov., a Nonsporulating Thermophilic Bacterium from a Japanese Thermal Spa

1974 • 609 citations

Hydrophobic stabilization in T4 lysozyme determined directly by multiple substitutions of Ile 3

1988 • 375 citations

Energetics of complementary side chain packing in a protein hydrophobic core

1989 • 346 citations

Genetic transformation of the extreme thermophile Thermus thermophilus and of other Thermus spp

1986 • 315 citations

Enhanced protein thermostability from designed mutations that interact with α-helix dipoles

1988 • 290 citations

Dependence of conformational stability on hydrophobicity of the amino acid residue in a series of variant proteins substituted at a unique position of tryptophan synthase alpha subunit.

1987 • 247 citations

Stabilization of phage T4 lysozyme by engineered disulfide bonds.

1989 • 245 citations

High guanine plus cytosine content in the third letter of codons of an extreme thermophile. DNA sequence of the isopropylmalate dehydrogenase of Thermus thermophilus.

1984 • 207 citations

Three-dimensional structure of a highly thermostable enzyme, 3-isopropylmalate dehydrogenase of Thermus thermophilus at 2.2 Å resolution

1991 • 196 citations

GENETIC ANALYSIS OF STAPHYLOCOCCAL NUCLEASE: IDENTIFICATION OF THREE INTRAGENIC "GLOBAL" SUPPRESSORS OF NUCLEASE-MINUS MUTATIONS

1985 • 189 citations

Influence of Interior Packing and Hydrophobicity on the Stability of a Protein

1989 • 173 citations

The engineering of binding affinity at metal ion binding sites for the stabilization of proteins: subtilisin as a test case

1988 • 171 citations

Yeast LEU2. Repression of mRNA levels by leucine and primary structure of the gene product.

1984 • 152 citations

Cloning of 3-Isopropylmalate Dehydrogenase Gene of an Extreme Thermophile and Partial Purification of the Gene Product

1981 • 133 citations

Hydrophobic interaction at the subunit interface contributes to the thermostability of 3‐isopropylmalate dehydrogenase from an extreme thermophile, Thermus thermophilus

1994 • 131 citations

Amino Acid Residues Stabilizing a Bacillus α-Amylase against Irreversible Thermoinactivation

1989 • 129 citations

Control of oligomeric enzyme thermostability by protein engineering.

1987 • 113 citations

Isolation and characterization of a bacteriophage infectious to an extreme thermophile, Thermus thermophilus HB8

1975 • 101 citations

Design and creation of a Ca2+ binding site in human lysozyme to enhance structural stability.

1989 • 98 citations

Dramatic thermostabilization of yeast iso-1-cytochrome c by an asparagine----isoleucine replacement at position 57.

1989 • 95 citations

Purification, Catalytic Properties, and Thermal Stability of Threo-Ds-3-Isopropylmalate Dehydrogenase Coded by leuB Gene from an Extreme Thermophile, Thermus thermophilus Strain HB81

1990 • 81 citations

Directed mutagenesis in an asporogenous methylotrophic yeast: cloning, sequencing, and one-step gene disruption of the 3-isopropylmalate dehydrogenase gene (LEU2) of Candida boidinii to derive doubly auxotrophic marker strains

1992 • 49 citations

The Yarrowia lipolytica LEU2 gene

1987 • 49 citations

Screening of stable proteins in an extreme thermophile, Thermus thermophilus

1995 • 47 citations

Increasing and decreasing protein stability: Effects of revertant substitutions on the thermal denaturation of phage λ repressor

1985 • 42 citations

Thermal stability of chimeric isopropylmalate dehydrogenase genes constructed from a thermophile and a mesophile

1995 • 39 citations

Nucleotide sequencing analysis of a LEU gene of Candida maltosa which complements leuB mutation of Escherichia coli and leu2 mutation of Saccharomyces cerevisiae

1987 • 36 citations

The nucleotide sequence of 3-isopropylmalate dehydrogenase gene fromBacillus subtilis

1987 • 33 citations

DNA and amino-acid sequences of 3-isopropylmalate dehydrogenase of Bacillus coagulans. Comparison with the enzymes of Saccharomyces cerevisiae and Thermus thermophilus

1986 • 29 citations

Molecular Cloning and Nucleotide Sequence of 3-Isopropylmalate Dehydrogenase Gene (leuB) from an Extreme Thermophile, Thermus aquaticus YT-11

1991 • 29 citations

Molecular Cloning and Nucleotide Sequence of the 3-Isopropylmalate Dehydrogenase Gene of Candida Utilis

1987 • 26 citations

Recombination-Deficient Mutants of an Extreme Thermophile, Thermus thermophilus

1993 • 24 citations

The nucleotide sequence of 3-isopropylmalate dehydrogenase gene fromBacillus caldotenax

1987 • 22 citations

A stable intermediate in the thermal unfolding process of a chimeric 3‐isopropylmalate dehydrogenase between a thermophilic and a mesophilic enzymes

1996 • 19 citations

Three-dimensional structures of chimeric enzymes between Bacillus subtilis and Thermus thermophilus 3-isopropylmalate dehydrogenases

1994 • 13 citations

The nucleotide sequence of leuB from Salmonella typhimurium

1992 • 8 citations

Molecular cloning and sequencing of the -isopropylmalate dehydrogenase gene from the cyanobacterium Spirulina platensis

1992 • 8 citations