Low Fidelity Mutants in the O-Helix of Thermus aquaticus DNA Polymerase I

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

1989 • 7,496 citations

DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution.

1994 • 1,274 citations

Randomization of genes by PCR mutagenesis.

1992 • 1,056 citations

Fidelity of HIV-1 Reverse Transcriptase

1988 • 919 citations

Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP

1985 • 855 citations

Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase

1988 • 814 citations

Misreading of DNA templates containing 8-hydroxydeoxyguanosine at the modified base and at adjacent residues

1987 • 778 citations

An attempt to unify the structure of polymerases

1990 • 640 citations

FUNCTION AND STRUCTURE RELATIONSHIPS IN DNA POLYMERASES

1994 • 611 citations

Compilation, alignment, and phylogenetic relationships of DNA polymerases

1993 • 590 citations

Structure of DNA Polymerase I Klenow Fragment Bound to Duplex DNA

1993 • 473 citations

High fidelity DNA synthesis by theThermus aquaticusDNA polymerase

1990 • 393 citations

Isolation, Characterization, and Expression in Escherichia coli of the DNA Polymerase Gene from Thermus aquaticus

1989 • 370 citations

Crystal structure of Thermus aquaticus DNA polymerase

1995 • 355 citations

A single residue in DNA polymerases of the Escherichia coli DNA polymerase I family is critical for distinguishing between deoxy- and dideoxyribonucleotides.

1995 • 348 citations

DNA polymerase insertion fidelity. Gel assay for site-specific kinetics.

1987 • 332 citations

Structure of Taq polymerase with DNA at the polymerase active site

1996 • 322 citations

The mutational specificity of DNA polymerase-beta during in vitro DNA synthesis. Production of frameshift, base substitution, and deletion mutations.

1985 • 317 citations

Specificity and Mechanism of Error-prone Replication by Human Immunodeficiency Virus-1 Reverse Transcriptase

1989 • 316 citations

High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity.

1993 • 286 citations

A general structure for DNA-dependent DNA polymerases

1991 • 229 citations

Side chains involved in catalysis of the polymerase reaction of DNA polymerase I from Escherichia coli.

1992 • 194 citations

Polymerase structures and function: variations on a theme?

1995 • 192 citations

Reverse chemical mutagenesis: identification of the mutagenic lesions resulting from reactive oxygen species-mediated damage to DNA.

1994 • 189 citations

The mutational specificity of DNA polymerases-alpha and -gamma during in vitro DNA synthesis.

1985 • 176 citations

The Infidelity of Avian Myeloblastosis Virus Deoxyribonucleic Acid Polymerase in Polynucleotide Replication

1974 • 172 citations

Nucleotide sequence of the Escherichia coli polA gene and primary structure of DNA polymerase I.

1982 • 169 citations

On the fidelity of DNA replication. Effect of divalent metal ion activators and deoxyrionucleoside triphosphate pools on in vitro mutagenesis.

1979 • 151 citations

Differential extension of 3′ mispairs is a major contribution to the high fidelity of calf thymus DNA polymerase-α

1989 • 129 citations

A mutant of DNA polymerase I (Klenow fragment) with reduced fidelity

1991 • 124 citations

Reduced Frameshift Fidelity and Processivity of HIV-1 Reverse Transcriptase Mutants Containing Alanine Substitutions in Helix H of the Thumb Subdomain

1995 • 121 citations

Fidelity of mammalian DNA replication and replicative DNA polymerases

1991 • 119 citations

Deoxynucleoside Triphosphate and Pyrophosphate Binding Sites in the Catalytically Competent Ternary Complex for the Polymerase Reaction Catalyzed by DNA Polymerase I (Klenow Fragment)

1995 • 114 citations

Mammalian DNA polymerase beta can substitute for DNA polymerase I during DNA replication in Escherichia coli.

1992 • 99 citations

Random mutagenesis of Thermus aquaticus DNA polymerase I: concordance of immutable sites in vivo with the crystal structure.

1996 • 80 citations

NMR studies of the conformations and interactions of substrates and ribonucleotide templates bound to the large fragment of DNA polymerase I

1986 • 76 citations

Motif A of bacteriophage T4 DNA polymerase: role in primer extension and DNA replication fidelity. Isolation of new antimutator and mutator DNA polymerases.

1994 • 74 citations

Asp537, Asp812 are essential and Lys631, His811 are catalytically significant in bacteriophage T7 RNA polymerase activity

1992 • 72 citations

Changes in DNA base sequence induced by gamma-ray mutagenesis of lambda phage and prophage.

1988 • 72 citations

A novel ochre mutation in the β-thalassemia gene of a Thai

1989 • 59 citations

Photoaffinity labeling of the thymidine triphosphate binding domain in Escherichia coli DNA polymerase I: identification of histidine-881 as the site of cross-linking

1987 • 59 citations

Mechanisms of mutation by oxidative DNA damage: Reduced fidelity of mammalian DNA polymerase .beta.

1993 • 58 citations

Multi-stage proofreading in DNA replication

1993 • 56 citations

Oxygen radical induced mutagenesis is DNA polymerase specific

1994 • 56 citations

Fidelity studies of the human DNA polymerase alpha. The most conserved region among alpha-like DNA polymerases is responsible for metal-induced infidelity in DNA synthesis

1993 • 53 citations

Significance of the O-Helix Residues of Escherichia coli DNA Polymerase I in DNA Synthesis: Dynamics of the dNTP Binding Pocket

1996 • 43 citations

On the fidelity of DNA replication. Nucleoside monophosphate generation during polymerization.

1981 • 43 citations

Misincorporation during DNA synthesis, analyzed by gel electrophoresis

1984 • 40 citations

Bacteriophage T7 RNA Polymerase and Its Active-site Mutants

1994 • 36 citations

Unnatural Nucleotide Sequences in Biopharmaceutics

1996 • 6 citations