In Vitro Selection for Enzymatic Activity: A Model Study Using Adenylate Cyclase

Filamentous Fusion Phage: Novel Expression Vectors That Display Cloned Antigens on the Virion Surface

1985 • 3,886 citations

Phage antibodies: filamentous phage displaying antibody variable domains

1990 • 2,346 citations

Measurements of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assay

1985 • 1,241 citations

Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains

1991 • 1,014 citations

Combinatorial and Evolution-Based Methods in the Creation of Enantioselective Catalysts

2001 • 578 citations

Structural basis for the activation of anthrax adenylyl cyclase exotoxin by calmodulin

2002 • 418 citations

Hormone phage: An enrichment method for variant proteins with altered binding properties

1990 • 360 citations

Proteolytic selection for protein folding using filamentous bacteriophages

1998 • 262 citations

Chemical Selection for Catalysis in Combinatorial Antibody Libraries

1997 • 208 citations

Molecular evolution of proteins on filamentous phage. Mimicking the strategy of the immune system.

1992 • 201 citations

Selecting proteins with improved stability by a phage-based method

1998 • 193 citations

Thermostable DNA Polymerases

1996 • 178 citations

A method for directed evolution and functional cloning of enzymes

1998 • 139 citations

Man-made enzymes — from design to in vitro compartmentalisation

2000 • 130 citations

Selection of β-Lactamase on Filamentous Bacteriophage by Catalytic Activity

1994 • 124 citations

Selection for improved subtiligases by phage display

1999 • 122 citations

A strategy for the isolation of catalytic activities from repertoires of enzymes displayed on phage 1 1Edited by J. Karn

1999 • 105 citations

Glutathione Transferases with Novel Active Sites Isolated by Phage Display from a Library of Random Mutants

1995 • 101 citations

Phage display of a catalytic antibody to optimize affinity for transition-state analog binding

1997 • 95 citations

Turnover-based in vitro selection and evolution of biocatalysts from a fully synthetic antibody library

2003 • 87 citations

Direct selection for a catalytic mechanism from combinatorial antibody libraries.

1994 • 86 citations

A Method for the Selection of Catalytic Activity Using Phage Display and Proximity Coupling

1999 • 85 citations

Evolving catalytic antibodies in a phage-displayed combinatorial library

1998 • 77 citations

Insertional mutagenesis of Bordetella pertussis adenylate cyclase.

1992 • 68 citations

In vitro abzyme evolution to optimize antibody recognition for catalysis

2001 • 64 citations

Making chemistry selectable by linking it to infectivity

1997 • 63 citations

Mechanism-Based Phage Display Selection of Active-Site Mutants of Human Glutathione Transferase A1-1 Catalyzing S_NAr Reactions

1997 • 53 citations

Phage display selection of peptides possessing aldolase activity

2001 • 44 citations

Display of active subtilisin 309 on phage: analysis of parameters influencing the selection of subtilisin variants with changed substrate specificity from libraries using phosphonylating inhibitors 1 1Edited by A. R. Fersht

2000 • 40 citations

Phage display selects for amylases with improved low pH starch-binding

2002 • 36 citations

In vitro selection of enzymatically active lipase variants from phage libraries using a mechanism-based inhibitor

2001 • 35 citations

Selection of Metalloenzymes by Catalytic Activity Using Phage Display and Catalytic Elution

2001 • 32 citations

Selection of β-lactamases and penicillin binding mutants from a library of phage displayed TEM-1 β-lactamase randomly mutated in the active site Ω-loop 1 1Edited by A. R. Fersht

2000 • 32 citations

Novel concepts for selection of catalytic activity

2001 • 30 citations

In vitro selection for catalytic turnover from a library of β-lactamase mutants and penicillin-binding proteins

1997 • 16 citations

Enzymatic synthesis of 3′:5′ cyclic AMP using Bordetella pertussis adenylate cyclase co-immobilized with calmodulin on agarose beads

1988 • 12 citations

Efficient Display of Two Enzymes on Filamentous Phage Using an Improved Signal Sequence

2003 • 11 citations

[23] Use of phage display and transition-state analogs to select enzyme variants with altered catalytic properties: Glutathione transferase as an example

2000 • 10 citations