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Membrane Topology and Insertion of Membrane Proteins: Search for Topogenic Signals

Data up to Jan 2025

Published2000
Citations194
References220

Total Citations Per Year

Abstract

References (220)

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Overexpression of integral membrane proteins for structural studies

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Escherichia coli alkaline phosphatase fails to acquire disulfide bonds when retained in the cytoplasm

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The SecDFyajC domain of preprotein translocase controls preprotein movement by regulating SecA membrane cycling

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Protein Translocation in the Three Domains of Life: Variations on a Theme

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Bovine opsin has more than one signal sequence

1985 • 186 citations

Insertion of a multispanning membrane protein occurs sequentially and requires only one signal sequence

1988 • 185 citations

GTP binding and hydrolysis by the signal recognition particle during initiation of protein translocation

1993 • 183 citations

Identification of Acetylcholine Receptor Channel-Lining Residues in the M1 Segment of the .alpha.-Subunit

1995 • 179 citations

Mapping the Ends of Transmembrane Segments in a Polytopic Membrane Protein

1997 • 178 citations

Genetic analysis of the membrane insertion and topology of MalF, a cytoplasmic membrane protein of Escherichia coli

1988 • 175 citations

Transmembrane Topology of Glucose-6-Phosphatase

1998 • 170 citations

Structure of the biotinyl domain of acetyl-coenzyme A carboxylase determined by MAD phasing

1995 • 169 citations

The Sec system

1998 • 164 citations

Membrane Topology of the Human Na+/Glucose Cotransporter SGLT1

1996 • 164 citations

Topological ?frustration? in multispanning E. coli inner membrane proteins

1994 • 162 citations

Determination of External Loop Topology in the Serotonin Transporter by Site-directed Chemical Labeling

1998 • 162 citations

FtsY, the Prokaryotic Signal Recognition Particle Receptor Homologue, Is Essential for Biogenesis of Membrane Proteins

1997 • 155 citations

Biotinylation of Single Cysteine Mutants of the Glutamate Transporter GLT-1 from Rat Brain Reveals Its Unusual Topology

1998 • 154 citations

Determination of the Transmembrane Topology of Yeast Sec61p, an Essential Component of the Endoplasmic Reticulum Translocation Complex

1996 • 152 citations

Biogenesis of the Gram-Negative Bacterial Envelope

1997 • 152 citations

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1977 • 148 citations

Topological Rules for Membrane Protein Assembly in Eukaryotic Cells

1997 • 147 citations

Analysis of protein localization by use of gene fusions with complementary properties

1990 • 147 citations

Nucleotide sequence of the osmoregulatory proU operon of Escherichia coli

1989 • 146 citations

Membrane topology of the ArsB protein, the membrane subunit of an anion-translocating ATPase.

1992 • 146 citations

Amino acid residues lining the chloride channel of the cystic fibrosis transmembrane conductance regulator.

1994 • 146 citations

A vector for the construction of translational fusions to TEM β-lactamase and the analysis of protein export signals and membrane protein topology

1986 • 146 citations

Sec dependent and sec independent assembly of E. coli inner membrane proteins: the topological rules depend on chain length.

1993 • 145 citations

Membrane Protein Structure

1994 • 145 citations

Site-specific photocross-linking reveals that Sec61p and TRAM contact different regions of a membrane-inserted signal sequence.

1993 • 144 citations

Interaction of picrotoxin with GABAA receptor channel-lining residues probed in cysteine mutants

1995 • 143 citations

Transmembrane Organization of Mouse P-glycoprotein Determined by Epitope Insertion and Immunofluorescence

1996 • 142 citations

Nucleotide sequence of the alkaline phosphatase gene of Escherichia coli

1986 • 140 citations

Transmembrane Topography of Subunit a in theEscherichia coli F1F0 ATP Synthase

1998 • 138 citations

Genetic analysis of membrane protein topology by a sandwich gene fusion approach.

1990 • 138 citations

The Membrane Topology of Human Transient Receptor Potential 3 as Inferred from Glycosylation-scanning Mutagenesis and Epitope Immunocytochemistry

1998 • 136 citations

Mapping of cystic fibrosis transmembrane conductance regulator membrane topology by glycosylation site insertion

1994 • 134 citations

Substrate recognition by oligosaccharyltransferase. Studies on glycosylation of modified Asn-X-Thr/Ser tripeptides.

1983 • 133 citations

Expression, biotinylation and purification of a biotin-domain peptide from the biotin carboxy carrier protein of Escherichia coli acetyl-CoA carboxylase

1994 • 133 citations

Regulation by the ribosome of the GTPase of the signal-recognition particle during protein targeting

1996 • 132 citations

β‐lactamase as a probe of membrane protein assembly and protein export

1990 • 128 citations

Differential use of the signal recognition particle translocase targeting pathway for inner membrane protein assembly in Escherichia coli

1998 • 128 citations

Topology of the Glut 1 glucose transporter deduced from glycosylation scanning mutagenesis.

1994 • 127 citations

Membrane Topology of the MotA Protein ofEscherichia coil

1995 • 126 citations

Stepwise Movement of Preproteins in the Process of Translocation across the Cytoplasmic Membrane of Escherichia coli

1995 • 126 citations

Biogenesis of Polytopic Membrane Proteins: Membrane Segments Assemble within Translocation Channels prior to Membrane Integration

1996 • 123 citations

Co- and Posttranslational Translocation Mechanisms Direct Cystic Fibrosis Transmembrane Conductance Regulator N Terminus Transmembrane Assembly

1998 • 120 citations

The intracellular targeting and membrane topology of 3-hydroxy-3-methylglutaryl-CoA reductase.

1992 • 119 citations

Membrane Topology of Subunit a of the F1F0 ATP Synthase as Determined by Labeling of Unique Cysteine Residues

1998 • 117 citations

Asparagine-linked oligosaccharides are localized to single extracytosolic segments in multi-span membrane glycoproteins

1994 • 113 citations

Amino acids lining the channel of the gamma-aminobutyric acid type A receptor identified by cysteine substitution.

1993 • 112 citations

Systematic analysis of stop-transfer sequence for microsomal membrane

1991 • 112 citations

Use of phoA fusions to study the topology of the Escherichia coli inner membrane protein leader peptidase

1989 • 111 citations

Construction of a functional lactose permease devoid of cysteine residues

1991 • 111 citations

A Reentrant Loop Domain in the Glutamate Carrier EAAT1 Participates in Substrate Binding and Translocation

1998 • 111 citations

The use of gene fusions to determine the topology of all of the subunits of the cytochrome o terminal oxidase complex of Escherichia coli.

1990 • 111 citations

Sec61p is adjacent to nascent type I and type II signal-anchor proteins during their membrane insertion.

1993 • 109 citations

Membrane topology of the pBR322 tetracycline resistance protein. TetA-PhoA gene fusions and implications for the mechanism of TetA membrane insertion.

1992 • 109 citations

The topological analysis of integral cytoplasmic membrane proteins

1993 • 106 citations

Insertion of the Polytopic Membrane Protein MalF Is Dependent on the Bacterial Secretion Machinery

1996 • 102 citations

Cloning and Membrane Topology of a P type ATPase from Helicobacter pylori

1996 • 101 citations

The Membrane Topology of GAT-1, a (Na++ Cl−)-coupled γ-Aminobutyric Acid Transporter from Rat Brain

1997 • 99 citations

Forced Transmembrane Orientation of Hydrophilic Polypeptide Segments in Multispanning Membrane Proteins

1998 • 98 citations

Topological analysis of H+,K(+)-ATPase using in vitro translation

1994 • 94 citations

Different positively charged amino acids have similar effects on the topology of a polytopic transmembrane protein in Escherichia coli.

1992 • 94 citations

Analysis of the topology of a membrane protein by using a minimum number of alkaline phosphatase fusions

1993 • 92 citations

Membrane Topology of the 60-kDa Oxa1p Homologue fromEscherichia coli

1998 • 91 citations

Properties and purification of an active biotinylated lactose permease from Escherichia coli.

1993 • 89 citations

Topology of the Region Surrounding Glu681 of Human AE1 Protein, the Erythrocyte Anion Exchanger

1998 • 89 citations

Membrane Topology of P-Glycoprotein As Determined by Epitope Insertion: Transmembrane Organization of the N-Terminal Domain of mdr3

1995 • 86 citations

TnphoA and TnphoA' elements for making and switching fusions for study of transcription, translation, and cell surface localization

1992 • 86 citations

The transmembrane topology of the amino terminus of the alpha subunit of the nicotinic acetylcholine receptor

1991 • 84 citations

The transmembrane topology of the sn‐glycerol‐3‐phosphate permease of Escherichia coli analysed by phoA and lacZ protein fusions

1988 • 83 citations

Folding and assembly of bacterial alkaline phosphatase in vitro and in vivo.

1993 • 77 citations

Biogenesis and transmembrane topology of the CHIP28 water channel at the endoplasmic reticulum.

1994 • 77 citations

Residues in the pathway through a membrane transporter.

1995 • 74 citations

Membrane Topology of the Melibiose Permease ofEscherichiacoliStudied bymelB−phoAFusion Analysis

1996 • 71 citations

Epitope Insertion Favors a Six Transmembrane Domain Model for the Carboxy-Terminal Portion of the Multidrug Resistance-Associated Protein

1998 • 70 citations

Positively charged amino acids placed next to a signal sequence block protein translocation more efficiently in Escherichia coli than in mammalian microsomes

1993 • 70 citations

Further Studies on the Properties of Liver Propionyl Coenzyme A Holocarboxylase Synthetase and the Specificity of Holocarboxylase Formation

1966 • 69 citations

Estimation of structural similarity of membrane proteins by hydropathy profile alignment

1998 • 69 citations

Membrane topology of multidrug resistance protein expressed in Escherichia coli. N-terminal domain.

1994 • 67 citations

Transport Processes in Eukaryotic and Prokaryotic Organisms

1996 • 67 citations

Analysis of the Transmembrane Topology of the Glycine Transporter GLYT1

1997 • 66 citations

Sec-independent translocation of a 100-residue periplasmic N-terminal tail in the E. coli inner membrane protein proW.

1994 • 66 citations

Topology of the Na+/Proline Transporter of Escherichia coli

1998 • 65 citations

The Membrane Topology of the Rat Sarcoplasmic and Endoplasmic Reticulum Calcium ATPases by in Vitro Translation Scanning

1995 • 64 citations

Membrane Topology of the Transposon 10-encoded Metal-Tetracycline/H+ Antiporter as Studied by Site-directed Chemical Labeling

1997 • 62 citations

The use of gene fusions to examine the membrane topology of the L-subunit of the photosynthetic reaction center and of the cytochrome b subunit of the bc1 complex from Rhodobacter sphaeroides

1991 • 61 citations

Membrane topography of ColE1 gene products: the immunity protein

1991 • 61 citations

Topological Analysis of NhaA, a Na+/H+ Antiporter from Escherichia coli

1996 • 61 citations

Stop‐transfer function of pseudo‐random amino acid segments during translocation across prokaryotic and eukaryotic membranes

1998 • 60 citations

A topological analysis of subunit alpha from Escherichia coli F1F0-ATP synthase predicts eight transmembrane segments.

1990 • 59 citations

Assessment of Topogenic Functions of Anticipated Transmembrane Segments of Human Band 3

1998 • 59 citations

GTP hydrolysis by complexes of the signal recognition particle and the signal recognition particle receptor.

1993 • 58 citations

Use of phoA and lacZ fusions to study the membrane topology of ProW, a component of the osmoregulated ProU transport system of Escherichia coli

1996 • 57 citations

Topology of NAT2, a Prototypical Example of a New Family of Amino Acid Transporters

1997 • 55 citations

Mutations eliminating the protein export function of a membrane-spanning sequence.

1994 • 53 citations

Role ofN-glycosylation in the expression of human band 3-mediated anion transport

1994 • 52 citations

Membrane Topology of the C-terminal Half of the Neuronal, Glial, and Bacterial Glutamate Transporter Family

1996 • 52 citations

Membrane topology of the high-affinity L-glutamate transporter (GLAST-1) of the central nervous system.

1996 • 51 citations

[23] Probing structure of neurotransmitter transporters by substituted-cysteine accessibility method

1998 • 50 citations

A Mutation in the Escherichia coli secY Gene That Produces Distinct Effects on Inner Membrane Protein Insertion and Protein Export

1998 • 49 citations

Molecular genetic analysis of membrane protein topology

1988 • 46 citations

Topological Determinants of Internal Transmembrane Segments in P-glycoprotein Sequences

1995 • 44 citations

The role of the ribosome-translocon complex in translation and assembly of polytopic membrane proteins

1998 • 43 citations

A chloroplast DNA deletion located in RNA polymerase gene rpoC2 in CMS lines of sorghum

1993 • 42 citations

Multiple residues specify external tetraethylammonium blockade in voltage-gated potassium channels

1995 • 42 citations

Biotinylation in vivo as a sensitive indicator of protein secretion and membrane protein insertion

1996 • 42 citations

Testing the Charge Difference Hypothesis for the Assembly of a Eucaryotic Multispanning Membrane Protein

1998 • 41 citations

[66] Analysis of microbial biotin proteins

1979 • 40 citations

Structure-Function Relationships in OxlT, the Oxalate/Formate Transporter of Oxalobacter formigenes

1998 • 40 citations

Insertional mutagenesis of hydrophilic domains in the lactose permease of Escherichia coli.

1992 • 39 citations

Topological localization of cysteine 74 in the GABA transporter, GAT1, and its importance in ion binding and permeation

1998 • 39 citations

Structural Requirements for Interruption of Protein Translocation across Rough Endoplasmic Reticulum Membrane1

1990 • 37 citations

Coupled Translocation Events Generate Topological Heterogeneity at the Endoplasmic Reticulum Membrane

1998 • 36 citations

Membrane topology analysis of Escherichia coli K-12 Mtr permease by alkaline phosphatase and beta-galactosidase fusions

1995 • 36 citations

Purification of two active fusion proteins of the Na+‐dependent citrate carrier of Klebsiella pneumoniae

1994 • 35 citations

Insertion of a Bacterial Secondary Transport Protein in the Endoplasmic Reticulum Membrane

1999 • 34 citations

Membrane Topology of the Sodium Ion-dependent Citrate Carrier of Klebsiella pneumoniae

1996 • 34 citations

In vivo membrane assembly of the E.coli polytopic protein, melibiose permease, occurs via a Sec-independent process which requires the protonmotive force.

1996 • 34 citations

Chapter 10 The lactose permease of Escherichia coli: Past, present and future

1996 • 34 citations

Beta-galactosidase gene fusions as probes for the cytoplasmic regions of subunits I and II of the membrane-bound cytochrome d terminal oxidase from Escherichia coli.

1988 • 33 citations

Reinitiation of Protein Translocation across the Endoplasmic Reticulum Membrane for the Topogenesis of Multispanning Membrane Proteins

1996 • 32 citations

Analysis of the Transmembrane Topology and Membrane Assembly of the GAT-1 γ-Aminobutyric Acid Transporter

1997 • 32 citations

Site-directed Chemical Modification of Cysteine-scanning Mutants as to Transmembrane Segment II and Its Flanking Regions of the Tn10-encoded Metal-Tetracycline/H+ Antiporter Reveals a Transmembrane Water-filled Channel

1998 • 31 citations

Membrane Topology of the Di- and Tripeptide Transport Protein ofLactococcus lactis

1997 • 31 citations

Gene fusion analysis of membrane protein topology: a direct comparison of alkaline phosphatase and beta-lactamase fusions

1994 • 30 citations

A topological model for the high‐affinity nickel transporter of Alcaligenes eutrophus

1994 • 30 citations

Comparative Topology Studies in Saccharomyces cerevisiae and in Escherichia coli

1996 • 28 citations

Topological analysis of the Rhodobacter capsulatus PucC protein and effects of C-terminal deletions on light-harvesting complex II

1996 • 28 citations

A topological model for the general aromatic amino acid permease, AroP, of Escherichia coli

1997 • 28 citations

Insertion of the Polytopic Membrane Protein Lactose Permease Occurs by Multiple Mechanisms

1995 • 27 citations

Signal sequence recognition and targeting of ribosomes to the endoplasmic reticulum by the signal recognition particle do not require GTP.

1994 • 27 citations

Identification of Membrane Insertion Sequences of the Rabbit Gastric Cholecystokinin-A Receptor by in VitroTranslation

1997 • 27 citations

Topology analysis of the colicin V export protein CvaA in Escherichia coli

1995 • 26 citations

An amphipathic sequence determinant of membrane protein topology.

1994 • 26 citations

Topological Model of the Rhodobacter capsulatus Light-Harvesting Complex I Assembly Protein LhaA (Previously Known as ORF1696)

1998 • 25 citations

Position-specific Asp-Lys pairing can affect signal sequence function and membrane protein topology.

1993 • 24 citations

Design of a membrane protein for site-specific proteolysis: properties of engineered factor Xa protease sites in the lactose permease of Escherichia coli

1995 • 24 citations

Membrane Topology of Helixes VII and XI in the Lactose Permease of Escherichia coli Studied by lacY-phoA Fusion Analysis and Site-Directed Spectroscopy

1995 • 23 citations

Topology of the phenylalanine-specific permease of Escherichia coli

1996 • 23 citations

Topology and function of the Na+/proline transporter of Escherichia coli, a member of the Na+/solute cotransporter family

1998 • 22 citations

Membrane topology of the L-rhamnose-H+ transport protein (RhaT) from enterobacteria.

1993 • 22 citations

Transmembrane Segment (TMS) VIII of the Na+/Citrate Transporter CitS Requires Downstream TMS IX for Insertion in the Escherichia coli Membrane

1999 • 22 citations

Purification and properties of the synthetase catalyzing the biotination of the aposubunit of transcarboxylase from Propionibacterium shermanii

1988 • 22 citations

Dissection of De Novo Membrane Insertion Activities of Internal Transmembrane Segments of ATP-Binding-Cassette Transporters: Toward Understanding Topological Rules for Membrane Assembly of Polytopic Membrane Proteins

1998 • 21 citations

Positively charged residues influence the degree of SecA dependence in protein translocation across the E. coli inner membrane

1994 • 20 citations

Membrane topology analysis of the Escherichia coli cytosine permease

1995 • 18 citations

In Vitro Biotinylation Provides Quantitative Recovery of Highly Purified Active Lactose Permease in a Single Step

1998 • 18 citations

The Protein Translocation Apparatus Contributes to Determining the Topology of an Integral Membrane Protein in Escherichia coli

1998 • 18 citations

Introductory Lecture:In VitroTranslation Analysis of Integral Membrane Proteins

1997 • 13 citations

Use of Gene Fusions to Determine Membrane Protein Topology

1994 • 12 citations

Glycosylation of multiple extracytosolic loops in Band 3, a model polytopic membrane protein

1996 • 11 citations

Assembly of eukaryotic class III (N‐out, C‐in) membrane proteins into the Escherichia coli cytoplasmic membrane

1993 • 11 citations

Mechanism Involved in Generating the Carboxyl-Terminal Half Topology of P-Glycoprotein

1998 • 10 citations

Translocation of proteins across the bacterial cytoplasmic membrane

1996 • 10 citations

Chapter 32 Translocation of proteins across the bacterial cytoplasmic membrane

1996 • 5 citations

Chapter 9 Molecular genetic analysis of membrane protein topology

1996 • 4 citations

[20] Analysis of transporter topology using deletion and epitope tagging

1998 • 3 citations

The Role of Charged Amino Acids Minireview in the Localization of Secreted and Membrane Proteins

1990 • 3 citations

Deleted Work

1955 • 0 citations

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