Topological Rules for Membrane Protein Assembly in Eukaryotic Cells

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

Intracellular protein topogenesis

1980 • 1,378 citations

A New Generation of Animal Cell Expression Vectors Based on the Semliki Forest Virus Replicon

1991 • 899 citations

A Model Recognition Approach to the Prediction of All-Helical Membrane Protein Structure and Topology

1994 • 790 citations

Topogenic signals in integral membrane proteins

1988 • 715 citations

Predicting the orientation of eukaryotic membrane-spanning proteins.

1989 • 596 citations

Control of topology and mode of assembly of a polytopic membrane protein by positively charged residues

1989 • 587 citations

Context Effects and Inefficient Initiation at Non-AUG Codons in Eucaryotic Cell-Free Translation Systems

1989 • 471 citations

In vitro mutagenesis of a full-length cDNA clone of Semliki Forest virus: the small 6,000-molecular-weight membrane protein modulates virus release

1991 • 450 citations

Determination of the distance between the oligosaccharyltransferase active site and the endoplasmic reticulum membrane.

1993 • 443 citations

Overexpression of integral membrane proteins for structural studies

1995 • 388 citations

Non-random Distribution of Amino Acids in the Transmembrane Segments of Human Type I Single Span Membrane Proteins

1993 • 378 citations

Membrane Proteins: From Sequence to Structure

1994 • 298 citations

Predicting the topology of eukaryotic membrane proteins

1993 • 272 citations

Internally located cleavable signal sequences direct the formation of Semliki Forest virus membrane proteins from a polyprotein precursor

1991 • 223 citations

Fine-tuning the topology of a polytopic membrane protein: Role of positively and negatively charged amino acids

1990 • 221 citations

A short amino-terminal segment of microsomal cytochrome P-450 functions both as an insertion signal and as a stop-transfer sequence.

1987 • 215 citations

Insertion of a multispanning membrane protein occurs sequentially and requires only one signal sequence

1988 • 185 citations

Functions of signal and signal-anchor sequences are determined by the balance between the hydrophobic segment and the N-terminal charge.

1992 • 180 citations

Expression of an Olfactory Receptor inEscherichiacoli: Purification, Reconstitution, and Ligand Binding

1996 • 166 citations

The heterodimeric association between the membrane proteins of Semliki Forest virus changes its sensitivity to low pH during virus maturation

1989 • 164 citations

Topological ?frustration? in multispanning E. coli inner membrane proteins

1994 • 162 citations

Topology of eukaryotic type II membrane proteins: Importance of N-terminal positively charged residues flanking the hydrophobic domain

1991 • 153 citations

Membrane protein topology: effects of delta mu H+ on the translocation of charged residues explain the ‘positive inside’ rule.

1994 • 148 citations

Sec dependent and sec independent assembly of E. coli inner membrane proteins: the topological rules depend on chain length.

1993 • 145 citations

Charged residues are major determinants of the transmembrane orientation of a signal-anchor sequence.

1991 • 144 citations

Towards a comparative anatomy of N-terminal topogenic protein sequences

1986 • 140 citations

Positive charges at the NH2 terminus convert the membrane-anchor signal peptide of cytochrome P-450 to a secretory signal peptide.

1988 • 135 citations

High-level expression of M13 gene II protein from an inducible polycistronic messenger RNA

1985 • 130 citations

Heads or tails — what determines the orientation of proteins in the membrane

1995 • 125 citations

Transmembrane orientation of signal-anchor proteins is affected by the folding state but not the size of the N-terminal domain.

1995 • 123 citations

Properties of N-terminal tails in G-protein coupled receptors: a statistical study

1995 • 113 citations

A 30-residue-long "export initiation domain" adjacent to the signal sequence is critical for protein translocation across the inner membrane of Escherichia coli.

1991 • 95 citations

The amino-terminal structures that determine topological orientation of cytochrome P-450 in microsomal membrane.

1990 • 90 citations

Structural requirements for membrane assembly of proteins spanning the membrane several times.

1989 • 79 citations

NH2-terminal substitutions of basic amino acids induce translocation across the microsomal membrane and glycosylation of rabbit cytochrome P450IIC2.

1989 • 73 citations

Positively charged amino acids placed next to a signal sequence block protein translocation more efficiently in Escherichia coli than in mammalian microsomes

1993 • 70 citations

The role of the polar, carboxyl-terminal domain of Escherichia coli leader peptidase in its translocation across the plasma membrane.

1986 • 68 citations

The role of the N region in signal sequence and signal-anchor function.

1992 • 65 citations

The cytoplasmic domain of Escherichia coli leader peptidase is a "translocation poison" sequence.

1988 • 61 citations

Role of NH2-terminal positively charged residues in establishing membrane protein topology.

1993 • 57 citations

The Role of Charged Residues in Determining Transmembrane Protein Insertion Orientation in Yeast

1996 • 45 citations

Topological Determinants of Internal Transmembrane Segments in P-glycoprotein Sequences

1995 • 44 citations

Sec‐independent protein insertion into the inner E. coli membrane A phenomenon in search of an explanation

1994 • 36 citations

SecA-dependence of the translocation of a large periplasmic loop in theEscherichia coliMalF inner membrane protein is a function of sequence context

1995 • 28 citations

Comparative Topology Studies in Saccharomyces cerevisiae and in Escherichia coli

1996 • 28 citations

Synergistic insertion of two hydrophobic regions drives Sec-independent membrane protein assembly.

1994 • 27 citations

Positively charged residues influence the degree of SecA dependence in protein translocation across the E. coli inner membrane

1994 • 20 citations

Assembly of eukaryotic class III (N‐out, C‐in) membrane proteins into the Escherichia coli cytoplasmic membrane

1993 • 11 citations

Deleted Work

1955 • 0 citations