Chapter 32 Translocation of proteins across the bacterial cytoplasmic membrane

Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold.

1982 • 5,284 citations

The signal peptide

1990 • 1,096 citations

Identification of a protein required for disulfide bond formation in vivo

1991 • 967 citations

Signal Sequence Recognition and Protein Targeting to the Endoplasmic Reticulum Membrane

1994 • 821 citations

Protein translocation into proteoliposomes reconstituted from purified components of the endoplasmic reticulum membrane

1993 • 654 citations

A protein-conducting channel in the endoplasmic reticulum

1991 • 613 citations

Control of topology and mode of assembly of a polytopic membrane protein by positively charged residues

1989 • 587 citations

The binding cascade of SecB to SecA to SecYE mediates preprotein targeting to the E. coli plasma membrane

1990 • 561 citations

The ATPase activity of secA is regulated by acidic phospholipids, secY, and the leader and mature domains of precursor proteins

1990 • 551 citations

SecA promotes preprotein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertion

1994 • 529 citations

The purified E. coli integral membrane protein is sufficient for reconstitution of SecA-dependent precursor protein translocation

1990 • 516 citations

Model for signal sequence recognition from amino-acid sequence of 54K subunit of signal recognition particle

1989 • 474 citations

Correlation of competence for export with lack of tertiary structure of the mature species: A study in vivo of maltose-binding protein in E. coli

1986 • 473 citations

ΔμH+ and ATP function at different steps of the catalytic cycle of preprotein translocase

1991 • 456 citations

A mammalian homolog of SEC61p and SECYp is associated with ribosomes and nascent polypeptides during translocation

1992 • 450 citations

THE ENZYMOLOGY OF PROTEIN TRANSLOCATION ACROSS THE Escherichia coli PLASMA MEMBRANE

1991 • 442 citations

What drives the translocation of proteins?

1992 • 415 citations

SecA protein hydrolyzes ATP and is an essential component of the protein translocation ATPase of Escherichia coli.

1989 • 406 citations

A protein complex required for signal-sequence-specific sorting and translocation

1994 • 404 citations

Secretion and Membrane Localization of Proteins inEscherichia Coli

1980 • 402 citations

Posttranslational protein transport in yeast reconstituted with a purified complex of Sec proteins and Kar2p

1995 • 400 citations

A pathway for disulfide bond formation in vivo.

1993 • 397 citations

Crystal structure of the DsbA protein required for disulphide bond formation in vivo

1993 • 387 citations

Suppressor mutations that restore export of a protein with a defective signal sequence

1981 • 382 citations

Transport of Proteins Across the Endoplasmic Reticulum Membrane

1992 • 366 citations

Homology of 54K protein of signal-recognition particle, docking protein and two E. coli proteins with putative GTP–binding domains

1989 • 357 citations

The antifolding activity of SecB promotes the export of the E. coli maltose-binding protein

1988 • 326 citations

Three pure chaperone proteins of Escherichia coli--SecB, trigger factor and GroEL--form soluble complexes with precursor proteins in vitro.

1989 • 317 citations

The APC Protein and E-cadherin Form Similar but Independent Complexes with α-Catenin, β-Catenin, and Plakoglobin

1995 • 315 citations

Azide-resistant mutants of Escherichia coli alter the SecA protein, an azide-sensitive component of the protein export machinery.

1990 • 311 citations

GENETIC ANALYSIS OF PROTEIN EXPORT IN ESCHERICHIA COLI

1990 • 291 citations

The signal sequence moves through a ribosomal tunnel into a noncytoplasmic aqueous environment at the ER membrane early in translocation

1993 • 286 citations

An E. coli Ribonucleoprotein Containing 4.5 S RNA Resembles Mammalian Signal Recognition Particle

1990 • 285 citations

Evolutionary conservation of components of the protein translocation complex

1994 • 283 citations

Early events in preprotein recognition in E. coli: interaction of SRP and trigger factor with nascent polypeptides.

1995 • 276 citations

Protein-specific energy requirements for protein transport across or into thylakoid membranes. Two lumenal proteins are transported in the absence of ATP.

1992 • 275 citations

A ribosome-associated peptidyl-prolyl cis/trans isomerase identified as the trigger factor.

1995 • 273 citations

The E. coli ffh gene is necessary for viability and efficient protein export

1992 • 268 citations

A new type of signal peptide: central role of a twin-arginine motif in transfer signals for the delta pH-dependent thylakoidal protein translocase.

1995 • 266 citations

FtsH is required for proteolytic elimination of uncomplexed forms of SecY, an essential protein translocase subunit.

1995 • 264 citations

Phosphatidylglycerol is involved in protein translocation across Escherichia coli inner membranes

1988 • 263 citations

Identification and characterization of an Escherichia coli gene required for the formation of correctly folded alkaline phosphatase, a periplasmic enzyme.

1992 • 259 citations

Trigger factor: a soluble protein that folds pro-OmpA into a membrane-assembly-competent form.

1987 • 257 citations

The protein-conducting channel in the membrane of the endoplasmic reticulum is open laterally toward the lipid bilayer

1995 • 253 citations

An alternative protein targeting pathway in Escherichia coli: studies on the role of FtsY.

1994 • 252 citations

Effects of mutations in heat-shock genes groES and groEL on protein export in Escherichia coli.

1989 • 250 citations

Transport of proteins into mitochondria: a potassium diffusion potential is able to drive the import of ADP/ATP carrier.

1985 • 245 citations

Purified secB protein of Escherichia coli retards folding and promotes membrane translocation of the maltose-binding protein in vitro.

1988 • 243 citations

The Escherichia coli dsbC (xprA) gene encodes a periplasmic protein involved in disulfide bond formation.

1994 • 241 citations

Systematic probing of the environment of a translocating secretory protein during translocation through the ER membrane.

1994 • 237 citations

Translocation of domains of nascent periplasmic proteins across the cytoplasmic membrane is independent of elongation

1983 • 234 citations

SecA interacts with secretory proteins by recognizing the positive charge at the amino terminus of the signal peptide in Escherichia coli.

1990 • 229 citations

A Kinetic Partitioning Model of Selective Binding of Nonnative Proteins by the Bacterial Chaperone SecB

1991 • 225 citations

Molecular Mechanisms of Protein Secretion: The Role of the Signal Sequence

1986 • 222 citations

A signal sequence is not required for protein export in prlA mutants of Escherichia coli.

1993 • 219 citations

Interaction of E. coli Ffh/4.5S ribonucleoprotein and FtsY mimics that of mammalian signal recognition particle and its receptor

1994 • 218 citations

Signal peptides open protein-conducting channels in E. coli

1992 • 216 citations

SecD and SecF facilitate protein export in Escherichia coli.

1994 • 215 citations

E. coli 4.5S RNA is part of a ribonucleoprotein particle that has properties related to signal recognition particle

1990 • 212 citations

Reconstitution of a protein translocation system containing purified SecY, SecE, and SecA from Escherichia coli.

1991 • 212 citations

Signal peptidases in prokaryotes and eukaryotes - a new protease family

1992 • 212 citations

SecA protein, a peripheral protein of the Escherichia coli plasma membrane, is essential for the functional binding and translocation of proOmpA.

1989 • 206 citations

Two distinct ATP‐binding domains are needed to promote protein export by Escherichia coli SecA ATPase

1993 • 197 citations

Characterization of membrane-associated and soluble states of SecA protein from wild-type and SecA51(TS) mutant strains of Escherichia coli.

1991 • 196 citations

ProOmpA is stabilized for membrane translocation by either purified E. coli trigger factor or canine signal recognition particle

1988 • 196 citations

SecD is involved in the release of translocated secretory proteins from the cytoplasmic membrane of Escherichia coli.

1993 • 190 citations

Signal-sequence recognition by an Escherichia coli ribonucleoprotein complex

1992 • 184 citations

Deep penetration of a portion of Escherichia coli SecA protein into model membranes is promoted by anionic phospholipids and by partial unfolding.

1992 • 183 citations

GTP binding and hydrolysis by the signal recognition particle during initiation of protein translocation

1993 • 183 citations

SecA Homolog in Protein Transport Within Chloroplasts: Evidence for Endosymbiont-Derived Sorting

1994 • 181 citations

Human SRP RNA and E. coli 4.5S RNA contain a highly homologous structural domain

1988 • 178 citations

The SecA and SecY subunits of translocase are the nearest neighbors of a translocating preprotein, shielding it from phospholipids.

1993 • 176 citations

Heat-shock proteins DnaK and GroEL facilitate export of LacZ hybrid proteins in E. coli

1990 • 175 citations

SecA insertion into phospholipids is stimulated by negatively charged lipids and inhibited by ATP: a monolayer study

1992 • 175 citations

Role for membrane potential in the secretion of protein into the periplasm of Escherichia coli.

1981 • 173 citations

Disruption of the gene encoding p12 (SecG) reveals the direct involvement and important function of SecG in the protein translocation of Escherichia coli at low temperature.

1994 • 171 citations

Characterization of DsbC, a periplasmic protein of Erwinia chrysanthemi and Escherichia coli with disulfide isomerase activity.

1994 • 170 citations

The secE gene encodes an integral membrane protein required for protein export in Escherichia coli.

1989 • 166 citations

ATP is essential for protein translocation into Escherichia coli membrane vesicles.

1985 • 163 citations

The “trigger factor cycle” includes ribosomes, presecretory proteins, and the plasma membrane

1988 • 162 citations

Complete nucleotide sequence of a gene encoding a functional human class I histocompatibility antigen (HLA-CW3).

1984 • 160 citations

Negatively charged phospholipids restore prePhoE translocation across phosphatidylglycerol-depleted Escherichia coli inner membranes

1991 • 159 citations

Physiological role during export for the retardation of folding by the leader peptide of maltose-binding protein.

1989 • 158 citations

SecA protein is exposed to the periplasmic surface of the E. coli inner membrane in its active state

1994 • 158 citations

SecA protein needs both acidic phospholipids and SecY/E protein for functional high-affinity binding to the Escherichia coli plasma membrane.

1991 • 150 citations

Procoat, the precursor of M13 coat protein, requires an electrochemical potential for membrane insertion.

1980 • 149 citations

The C Terminus of SecA Is Involved in Both Lipid Binding and SecB Binding

1995 • 147 citations

Sec dependent and sec independent assembly of E. coli inner membrane proteins: the topological rules depend on chain length.

1993 • 145 citations

Molecular chaperones and protein translocation across the Escherichia coli inner membrane

1991 • 143 citations

A novel membrane protein involved in protein translocation across the cytoplasmic membrane of Escherichia coli.

1993 • 143 citations

Translocation of ProOmpA possessing an intramolecular disulfide bridge into membrane vesicles of Escherichia coli. Effect of membrane energization.

1990 • 141 citations

No Specific Recognition of Leader Peptide by SecB, a Chaperone Involved in Protein Export

1990 • 141 citations

SecA, the peripheral subunit of the Escherichia coli precursor protein translocase, is functional as a dimer

1993 • 139 citations

Determination of a region in SecA that interacts with presecretory proteins in Escherichia coli.

1991 • 138 citations

Protein secretion in Pseudomonas aeruginosa

1992 • 136 citations

One of three transmembrane stretches is sufficient for the functioning of the SecE protein, a membrane component of the E. coli secretion machinery.

1991 • 136 citations

High selectivity with low specificity: how SecB has solved the paradox of chaperone binding

1995 • 134 citations

The requirement for energy during export of beta-lactamase in Escherichia coli is fulfilled by the total protonmotive force.

1984 • 132 citations

PrlA (SecY) and PrlG (SecE) interact directly and function sequentially during protein translocation in E. coli

1990 • 129 citations

Alterations in the extracellular domain of M13 procoat protein make its membrane insertion dependent on secA and secY

1988 • 128 citations

A gene (prsA) of Bacillus subtilis involved in a novel, late stage of protein export

1991 • 127 citations

Translocation can drive the unfolding of a preprotein domain.

1993 • 127 citations

Stepwise Movement of Preproteins in the Process of Translocation across the Cytoplasmic Membrane of Escherichia coli

1995 • 126 citations

Trigger factor depletion or overproduction causes defective cell division but does not block protein export

1990 • 125 citations

SecYEG and SecA Are the Stoichiometric Components of Preprotein Translocase

1995 • 125 citations

Reconstitution of an efficient protein translocation machinery comprising SecA and the three membrane proteins, SecY, SecE, and SecG (p12).

1994 • 125 citations

M13 procoat inserts into liposomes in the absence of other membrane proteins.

1985 • 121 citations

Peptide Binding by Chaperone SecB: Implications for Recognition of Nonnative Structure

1992 • 120 citations

Bacillus subtilis PrsA is required in vivo as an extracytoplasmic chaperone for secretion of active enzymes synthesized either with or without pro‐sequences

1993 • 119 citations

The beta subunit of the signal recognition particle receptor is a transmembrane GTPase that anchors the alpha subunit, a peripheral membrane GTPase, to the endoplasmic reticulum membrane.

1995 • 118 citations

Effects of Escherichia coli secB mutations on pre-maltose binding protein conformation and export kinetics.

1988 • 117 citations

Complementation of two overlapping fragments of SecA, a protein translocation ATPase of Escherichia coli, allows ATP binding to its amino-terminal region.

1990 • 115 citations

PrlA suppressor mutations cluster in regions corresponding to three distinct topological domains.

1993 • 115 citations

ProOmpA contains secondary and tertiary structure prior to translocation and is shielded from aggregation by association with SecB protein.

1990 • 114 citations

Identification of a gene required for maturation of an extracellular lactococcal serine proteinase

1989 • 114 citations

Precursor protein translocation by the Escherichia coli translocase is directed by the protonmotive force.

1992 • 113 citations

SecY, SecE, and band 1 form the membrane-embedded domain of Escherichia coli preprotein translocase.

1992 • 113 citations

SecE‐dependent overproduction of SecY in Escherichia coli

1990 • 111 citations

Protein secretion inPseudomonas aeruginosa

1992 • 111 citations

The Functional Integration of a Polytopic Membrane Protein of Escherichia coli is Dependent on the Bacterial Signal‐Recognition Particle

1995 • 111 citations

SecD and SecF are required for the proton electrochemical gradient stimulation of preprotein translocation.

1994 • 111 citations

The Cs sec mutants of Escherichia coli reflect the cold sensitivity of protein export itself.

1993 • 110 citations

SecA, an essential component of the secretory machinery of Escherichiacoli, exists as homodimer

1991 • 108 citations

SecB functions as a cytosolic signal recognition factor for protein export in E. coli

1989 • 107 citations

Specific recognition of the leader region of precursor proteins is required for the activation of translocation ATPase of Escherichia coli.

1989 • 107 citations

Proton Motive Force-dependent and -independent Protein Translocation Revealed by an Efficient in Vitro Assay System of Escherichia coli

1989 • 107 citations

Highly selective binding of nascent polypeptides by an Escherichia coli chaperone protein in vivo

1993 • 107 citations

The yeast SSS1 gene is essential for secretory protein translocation and encodes a conserved protein of the endoplasmic reticulum.

1993 • 107 citations

Trigger factor is involved in GroEL-dependent protein degradation in Escherichia coli and promotes binding of GroEL to unfolded proteins.

1995 • 106 citations

In vitro Analysis of the Process of Translocation of OmpA across the Escherichia coli Cytoplasmic Membrane

1989 • 103 citations

S‐Adenosylmethionine synthetase in the thermophilic archaebacterium Sulfolobus solfataricus.

1988 • 101 citations

The conformation of SecA, as revealed by its protease sensitivity, is altered upon interaction with ATP, presecretory proteins, everted membrane vesicles, and phospholipids.

1991 • 100 citations

Novel secA alleles improve export of maltose-binding protein synthesized with a defective signal peptide

1989 • 99 citations

The biosynthesis of membrane-bound M13 coat protein. Energetics and assembly intermediates.

1982 • 97 citations

Genetic and molecular characterization of the Escherichia coli secD operon and its products

1994 • 93 citations

In vivo analysis of integration of membrane proteins in Escherichia coli

1991 • 92 citations

A high Concentration of SecA Allows Proton Motive Force-independent Translocation of a Model Secretory Protein into Escherichia coli Membrane Vesicles

1989 • 92 citations

Mammalian and Escherichia coli signal recognition particles

1994 • 91 citations

SecA protein: Autoregulated initiator of secretory precursor protein translocation across theE. coli plasma membrane

1990 • 87 citations

Bacterial protein disulfide isomerase: Efficient catalysis of oxidative protein folding at acidic pH

1993 • 86 citations

The proton motive force lowers the level of ATP required for the in vitro translocation of a secretory protein in Escherichia coli.

1990 • 85 citations

SecA protein: autoregulated ATPase catalysing preprotein insertion and translocation across the Escherichia coli inner membrane

1993 • 85 citations

Determinants of the quantity of the stable SecY complex in the Escherichia coli cell

1993 • 83 citations

Overproduction, purification and characterization of SecD and SecF, integral membrane components of the protein translocation machinery of Escherichia coli

1992 • 82 citations

Conservative sorting of F0-ATPase subunit 9: export from matrix requires delta pH across inner membrane and matrix ATP.

1995 • 79 citations

PrlA and PrlG suppressors reduce the requirement for signal sequence recognition

1994 • 77 citations

A Monomeric, Tightly Folded Stromal Intermediate on the △pH-dependent Thylakoidal Protein Transport Pathway

1995 • 76 citations

Heat-shock proteins can substitute for SecB function during protein export in Escherichia coli.

1991 • 73 citations

Proton transfer is rate-limiting for translocation of precursor proteins by the Escherichia coli translocase.

1991 • 70 citations

The carboxyl-terminal region of SecE interacts with SecY and is functional in the reconstitution of protein translocation activity in Escherichia coli.

1992 • 68 citations

Residues essential for the function of SecE, a membrane component of the Escherichia coli secretion apparatus, are located in a conserved cytoplasmic region.

1994 • 68 citations

Characterization of a Bacillus subtilis SecA mutant protein deficient in translocation ATPase and release from the membrane

1993 • 68 citations

Suppressor analysis suggests a multistep, cyclic mechanism for protein secretion in Escherichia coli.

1992 • 65 citations

Genetic analysis of SecY: additional export-defective mutations and factors affecting their phenotypes

1994 • 65 citations

Time of action of 4·5 S RNA in Escherichia coli translation

1989 • 64 citations

Efficient translocation of positively charged residues of M13 procoat protein across the membrane excludes electrophoresis as the primary force for membrane insertion.

1990 • 63 citations

Diffusion-Limited Interaction between Unfolded Polypeptides and the Escherichia coli Chaperone SecB

1995 • 62 citations

Subunit dynamics in Escherichia coli preprotein translocase.

1994 • 61 citations

OmpF-Lpp signal sequence mutants with varying charge hydrophobicity ratios provide evidence for a phosphatidylglycerol-signal sequence interaction during protein translocation across the Escherichia coli inner membrane

1993 • 61 citations

Translocation of Pro-OmpA across Inner Membrane Vesicles of Escherichia coli Occurs in Two Consecutive Energetically Distinct Steps

1989 • 60 citations

Suppression of signal sequence defects and azide resistance in Escherichia coli commonly result from the same mutations in secA

1995 • 60 citations

A dual role for phosphatidylglycerol in protein translocation across the Escherichia coli inner membrane.

1994 • 59 citations

Effects of total hydrophobicity and length of the hydrophobic domain of a signal peptide on in vitro translocation efficiency.

1992 • 58 citations

The overexpressed human 46-kDa mannose 6-phosphate receptor mediates endocytosis and sorting of beta-glucuronidase.

1990 • 58 citations

A cytoplasmic domain is important for the formation of a SecY-SecE translocator complex.

1994 • 57 citations

Identification of a gene fragment which codes for the 364 amino-terminal amino acid residues of a SecA homologue from Bacillus subtilis: further evidence for the conservation of the protein export apparatus in gram-positive and gram-negative bacteria

1991 • 56 citations

Bacterial protein translocation: kinetic and thermodynamic role of ATP and the protonmotive force

1992 • 56 citations

The requirement of a positive charge at the amino terminus can be compensated for by a longer central hydrophobic stretch in the functioning of signal peptides.

1992 • 54 citations

Identification of a soluble SecA/SecB complex by means of a subfractionated cell-free export system.

1994 • 54 citations

Lysine 106 of the putative catalytic ATP-binding site of the Bacillus subtilis SecA protein is required for functional complementation of Escherichia coli secA mutants in vivo.

1993 • 54 citations

Mutations conferring resistance to azide in Escherichia coli occur primarily in the secA gene

1990 • 53 citations

Autogenous translation regulation by Escherichia coli ATPase SecA may be mediated by an intrinsic RNA helicase activity of this protein

1992 • 53 citations

SecY and integral membrane components of the Escherichia coli protein translocation system

1992 • 53 citations

The primary pathway of protein export in E. coli

1991 • 53 citations

Role of acidic lipids in the translocation and channel activity of colicins A and N in Escherichia coli cells

1993 • 46 citations

Charged residues render pro-OmpA potential dependent for initiation of membrane translocation

1993 • 45 citations

A new genetic selection identifies essential residues in SecG, a component of the Escherichia coli protein export machinery.

1995 • 44 citations

Identification of the Magnesium-binding Domain of the High-affinity ATP-binding Site of the Bacillus subtilis and Escherichia coli SecA Protein

1995 • 44 citations

Site-specific antibodies against the PrlA (secY) protein of Escherichia coli inhibit protein export by interfering with plasma membrane binding of preproteins.

1989 • 43 citations

SecA protein is required for translocation of a model precursor protein into inverted vesicles of Escherichia coli plasma membrane.

1993 • 43 citations

Purified Escherichia coli preprotein translocase catalyzes multiple cycles of precursor protein translocation

1993 • 42 citations

Solubilization and functional reconstitution of the protein-translocation enzymes of Escherichia coli.

1990 • 41 citations

Purification of SecE and reconstitution of SecE‐dependent protein translocation activity

1991 • 41 citations

Band 1 subunit of Escherichia coli preportein translocase and integral membrane export factor P12 are the same protein.

1994 • 41 citations

Protein translocation into Escherichia coli membrane vesicles is inhibited by functional synthetic signal peptides.

1987 • 40 citations

Reconstitution of translocation activity for secretory proteins from solubilized components of Escherichia coli

1990 • 40 citations

Acquisition of azide-resistance by elevated SecA ATPase activity confers azide-resistance upon cell growth and protein translocation in Bacillus subtilis

1995 • 40 citations

In vitro membrane assembly of a polytopic, transmembrane protein results in an enzymatically active conformation.

1989 • 39 citations

In‐vitro studies on the folding characteristics of the Escherichia coli precursor protein prePhoE

1992 • 38 citations

SecY variants that interfere with Escherichia coli protein export in the presence of normal secY

1992 • 37 citations

Membrane vesicles containing overproduced SecY and SecE exhibit high translocation ATPase activity and countermovement of protons in a SecA- and presecretory protein-dependent manner.

1993 • 37 citations

Sec‐independent protein insertion into the inner E. coli membrane A phenomenon in search of an explanation

1994 • 36 citations

Electrochemical potential releases a membrane-bound secretion intermediate of maltose-binding protein in Escherichia coli

1990 • 35 citations

Nucleotide and negatively charged lipid‐dependent vesicle aggregation caused by SecA

1993 • 33 citations

Effects of nucleotides on ATP-dependent protein translocation into Escherichia coli membrane vesicles

1986 • 33 citations

Overexpression of phosphatidylglycerophosphate synthase restores protein translocation in asecG deletion mutant ofEscherichia coli at low temperature

1995 • 32 citations

Mutations of the molecular chaperone protein SecB which alter the interaction between SecB and maltose-binding protein.

1993 • 32 citations

Regulation of Escherichia coli secA mRNA translation by a secretion-responsive element

1991 • 32 citations

SecB‐binding does not maintain the translocation‐competent state of prePhoE

1992 • 31 citations

Carboxy-Terminal Region of Escherichia coli SecA ATPase Is Important to Promote Its Protein Translocation Activity in Vivo

1994 • 29 citations

Preprotein translocation creates a halide anion permeability in the Escherichia coli plasma membrane.

1992 • 29 citations

A proline residue near the amino terminus of the mature domain of secretory proteins lowers the level of the proton motive force required for translocation

1991 • 29 citations

In vitro translocation of secretory proteins possessing no charges at the mature domain takes place efficiently in a protonmotive force-dependent manner.

1992 • 29 citations

A chemically cross‐linked nonlinear proOmpA molecule can be translocated into everted membrane vesicles of Escherichia coli in the presence of the proton motive force

1991 • 27 citations

Multiple SecA protein isoforms in Escherichia coli

1987 • 25 citations

Requirement for conformational flexibility in the signal sequence of precursor protein.

1994 • 23 citations

Elevated cytosolic concentrations of SecA compensate for a protein translocation defect in Escherichia coli cells with reduced levels of negatively charged phospholipids

1992 • 23 citations

The role of the mature domain of proOmpA in the translocation ATPase reaction.

1992 • 22 citations

Folding in vitro and transport in vivo of pre-beta-lactamase are SecB independent.

1991 • 22 citations

Folding in vitro and transport in vivo of pre‐β‐lactamase are SecB independent

1991 • 22 citations

Protein translocation across the ER membrane: a fluorescent light at the end of the tunnel

1993 • 20 citations

In vitro protein translocation into inverted membrane vesicles prepared from Vibrio alginolyticus is stimulated by the electrochemical potential of Na⁺ in the presence of Escherichia coli SecA

1990 • 19 citations

Biochemical analysis of the biogenesis and function of the Escherichia coli export factor SecY

1992 • 19 citations

A Truncated Bacillus subtilis SecA Protein Consisting of the N-Terminal 234 Amino Acid Residues Forms a Complex with Escherichia coli SecA51(ts) Protein and Complements the Protein Translocation Defect of the secA51 Mutant1

1994 • 19 citations

Export of periplasmic galactose-binding protein in Escherichia coli depends on the chaperone SecB

1995 • 16 citations

Translocation of conjugated presecretory proteins possessing an internal non-peptide domain into everted membrane vesicles in Escherichia coli.

1993 • 12 citations

Escherichia coli SecY and SecE proteins appear insufficient to constitute the SecA receptor

1994 • 10 citations