SecA promotes preprotein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertion

THE ATTRACTIONS OF PROTEINS FOR SMALL MOLECULES AND IONS

1949 • 19,979 citations

Surface-specific iodination of membrane proteins of viruses and eucaryotic cells using 1,3,4,6-tetrachloro-3α,6α-diphenylglycoluril

1978 • 912 citations

The binding cascade of SecB to SecA to SecYE mediates preprotein targeting to the E. coli plasma membrane

1990 • 561 citations

The ATPase activity of secA is regulated by acidic phospholipids, secY, and the leader and mature domains of precursor proteins

1990 • 551 citations

The purified E. coli integral membrane protein is sufficient for reconstitution of SecA-dependent precursor protein translocation

1990 • 516 citations

ΔμH+ and ATP function at different steps of the catalytic cycle of preprotein translocase

1991 • 456 citations

THE ENZYMOLOGY OF PROTEIN TRANSLOCATION ACROSS THE Escherichia coli PLASMA MEMBRANE

1991 • 442 citations

SecA protein hydrolyzes ATP and is an essential component of the protein translocation ATPase of Escherichia coli.

1989 • 406 citations

Three pure chaperone proteins of Escherichia coli--SecB, trigger factor and GroEL--form soluble complexes with precursor proteins in vitro.

1989 • 317 citations

In vivo evidence for the role of the epsilon subunit as an inhibitor of the proton-translocating ATPase of Escherichia coli

1984 • 309 citations

A signal sequence is not required for protein export in prlA mutants of Escherichia coli.

1993 • 219 citations

Detection of prokaryotic signal peptidase in an Escherichia coli membrane fraction: endoproteolytic cleavage of nascent f1 pre-coat protein.

1978 • 209 citations

SecA protein, a peripheral protein of the Escherichia coli plasma membrane, is essential for the functional binding and translocation of proOmpA.

1989 • 206 citations

Synthesis, assembly into the cytoplasmic membrane, and proteolytic processing of the precursor of coliphage M13 coat protein.

1980 • 200 citations

Two distinct ATP‐binding domains are needed to promote protein export by Escherichia coli SecA ATPase

1993 • 197 citations

Characterization of membrane-associated and soluble states of SecA protein from wild-type and SecA51(TS) mutant strains of Escherichia coli.

1991 • 196 citations

Nucleotide sequence of the secA gene and secA(Ts) mutations preventing protein export in Escherichia coli

1988 • 188 citations

Deep penetration of a portion of Escherichia coli SecA protein into model membranes is promoted by anionic phospholipids and by partial unfolding.

1992 • 183 citations

The SecA and SecY subunits of translocase are the nearest neighbors of a translocating preprotein, shielding it from phospholipids.

1993 • 176 citations

SecA insertion into phospholipids is stimulated by negatively charged lipids and inhibited by ATP: a monolayer study

1992 • 175 citations

secD, a new gene involved in protein export in Escherichia coli

1987 • 173 citations

Role for membrane potential in the secretion of protein into the periplasm of Escherichia coli.

1981 • 173 citations

SecA protein is exposed to the periplasmic surface of the E. coli inner membrane in its active state

1994 • 158 citations

Energy is Required for Maturation of Exported Proteins in Escherichia coli

1981 • 157 citations

SecA protein needs both acidic phospholipids and SecY/E protein for functional high-affinity binding to the Escherichia coli plasma membrane.

1991 • 150 citations

A novel membrane protein involved in protein translocation across the cytoplasmic membrane of Escherichia coli.

1993 • 143 citations

Both ATP and the electrochemical potential are required for optimal assembly of pro-OmpA into Escherichia coli inner membrane vesicles.

1986 • 142 citations

SecA, the peripheral subunit of the Escherichia coli precursor protein translocase, is functional as a dimer

1993 • 139 citations

ProOmpA spontaneously folds in a membrane assembly competent state which trigger factor stabilizes.

1988 • 135 citations

Translocation can drive the unfolding of a preprotein domain.

1993 • 127 citations

Escherichia coli SecB protein associates with exported protein precursors in vivo.

1989 • 123 citations

The membrane-bound proteins of periplasmic permeases form a complex. Identification of the histidine permease HisQMP complex

1991 • 119 citations

Assembly of translocation-competent proteoliposomes from detergent-solubilized rough microsomes

1990 • 118 citations

Mechanisms of membrane assembly: effects of energy poisons on the conversion of soluble M13 coliphage procoat to membrane-bound coat protein.

1980 • 115 citations

SecY, SecE, and band 1 form the membrane-embedded domain of Escherichia coli preprotein translocase.

1992 • 113 citations

SecA, an essential component of the secretory machinery of Escherichiacoli, exists as homodimer

1991 • 108 citations

In vitro Analysis of the Process of Translocation of OmpA across the Escherichia coli Cytoplasmic Membrane

1989 • 103 citations

The conformation of SecA, as revealed by its protease sensitivity, is altered upon interaction with ATP, presecretory proteins, everted membrane vesicles, and phospholipids.

1991 • 100 citations

Novel secA alleles improve export of maltose-binding protein synthesized with a defective signal peptide

1989 • 99 citations

A high Concentration of SecA Allows Proton Motive Force-independent Translocation of a Model Secretory Protein into Escherichia coli Membrane Vesicles

1989 • 92 citations

Immunological and Molecular Characterization of Goα-like Proteins in the Drosophila Central Nervous System

1989 • 90 citations

SecA protein: Autoregulated initiator of secretory precursor protein translocation across theE. coli plasma membrane

1990 • 87 citations

The proton motive force lowers the level of ATP required for the in vitro translocation of a secretory protein in Escherichia coli.

1990 • 85 citations

SecA protein: autoregulated ATPase catalysing preprotein insertion and translocation across the Escherichia coli inner membrane

1993 • 85 citations

The ATP-binding component of a prokaryotic traffic ATPase is exposed to the periplasmic (external) surface.

1993 • 69 citations

SecA suppresses the temperature-sensitive SecY24 defect in protein translocation in Escherichia coli membrane vesicles.

1988 • 62 citations

Regulation of the Escherichia coli secA gene by protein secretion defects: analysis of secA, secB, secD, and secY mutants

1988 • 56 citations

Characterization of Escherichia coli SecA protein binding to a site on its mRNA involved in autoregulation.

1991 • 43 citations

Purified Escherichia coli preprotein translocase catalyzes multiple cycles of precursor protein translocation

1993 • 42 citations

Band 1 subunit of Escherichia coli preportein translocase and integral membrane export factor P12 are the same protein.

1994 • 41 citations

Roles of H+-ATPase and proton motive force in ATP-dependent protein translocation in vitro

1986 • 39 citations

Membrane vesicles containing overproduced SecY and SecE exhibit high translocation ATPase activity and countermovement of protons in a SecA- and presecretory protein-dependent manner.

1993 • 37 citations

(3] Purification and characterization of leader peptidase from Escherichia coli

1983 • 31 citations

Preprotein translocation creates a halide anion permeability in the Escherichia coli plasma membrane.

1992 • 29 citations

Electron microscopy of thin-sectioned three-dimensional crystals of SecA protein from Escherichia coli: Structure in projection at 40 Å resolution

1992 • 17 citations