Sec dependent and sec independent assembly of E. coli inner membrane proteins: the topological rules depend on chain length.

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

Membrane protein structure prediction

1992 • 1,680 citations

The spontaneous insertion of proteins into and across membranes: The helical hairpin hypothesis

1981 • 868 citations

The distribution of positively charged residues in bacterial inner membrane proteins correlates with the trans-membrane topology

1986 • 861 citations

Topogenic signals in integral membrane proteins

1988 • 715 citations

Control of topology and mode of assembly of a polytopic membrane protein by positively charged residues

1989 • 587 citations

The binding cascade of SecB to SecA to SecYE mediates preprotein targeting to the E. coli plasma membrane

1990 • 561 citations

The ATPase activity of secA is regulated by acidic phospholipids, secY, and the leader and mature domains of precursor proteins

1990 • 551 citations

Transcending the impenetrable: How proteins come to terms with membranes

1988 • 471 citations

ΔμH+ and ATP function at different steps of the catalytic cycle of preprotein translocase

1991 • 456 citations

THE ENZYMOLOGY OF PROTEIN TRANSLOCATION ACROSS THE Escherichia coli PLASMA MEMBRANE

1991 • 442 citations

GENETIC ANALYSIS OF PROTEIN EXPORT IN ESCHERICHIA COLI K12

1985 • 432 citations

Azide-resistant mutants of Escherichia coli alter the SecA protein, an azide-sensitive component of the protein export machinery.

1990 • 311 citations

Sequence of the leader peptidase gene of Escherichia coli and the orientation of leader peptidase in the bacterial envelope.

1983 • 307 citations

Fine-tuning the topology of a polytopic membrane protein: Role of positively and negatively charged amino acids

1990 • 221 citations

Membrane proteins: from sequence to structure

1990 • 137 citations

Positively charged residues are important determinants of membrane protein topology

1990 • 132 citations

High-level expression of M13 gene II protein from an inducible polycistronic messenger RNA

1985 • 130 citations

Alterations in the extracellular domain of M13 procoat protein make its membrane insertion dependent on secA and secY

1988 • 128 citations

Positively charged amino acid residues can act as topogenic determinants in membrane proteins.

1989 • 122 citations

Characterization of cold-sensitive secY mutants of Escherichia coli

1990 • 107 citations

A 30-residue-long "export initiation domain" adjacent to the signal sequence is critical for protein translocation across the inner membrane of Escherichia coli.

1991 • 95 citations

Different positively charged amino acids have similar effects on the topology of a polytopic transmembrane protein in Escherichia coli.

1992 • 94 citations

A small hydrophobic domain anchors leader peptidase to the cytoplasmic membrane of Escherichia coli.

1987 • 90 citations

Bacterial leader peptidase, a membrane protein without a leader peptide, uses the same export pathway as pre-secretory proteins

1984 • 84 citations

Distinct domains of an oligotopic membrane protein are Sec-dependent and Sec-independent for membrane insertion.

1992 • 83 citations

Hydrophobic content and lipid interactions of wild-type and mutant OmpA signal peptides correlate with their in vivo function

1991 • 74 citations

Leader Peptidase of Escherichia coli : Critical Role of a Small Domain in Membrane Assembly

1987 • 72 citations

[1] Genetic analysis of protein export in Escherichia coli

1983 • 70 citations

Mapping of catalytically important domains in Escherichia coli leader peptidase.

1990 • 70 citations

The role of the polar, carboxyl-terminal domain of Escherichia coli leader peptidase in its translocation across the plasma membrane.

1986 • 68 citations

Suppressor analysis suggests a multistep, cyclic mechanism for protein secretion in Escherichia coli.

1992 • 65 citations

Efficient translocation of positively charged residues of M13 procoat protein across the membrane excludes electrophoresis as the primary force for membrane insertion.

1990 • 63 citations

The cytoplasmic domain of Escherichia coli leader peptidase is a "translocation poison" sequence.

1988 • 61 citations

Export of Escherichia coli Alkaline Phosphatase Attached to an Integral Membrane Protein, SecY

1989 • 60 citations

The Tsr chemosensory transducer of Escherichia coli assembles into the cytoplasmic membrane via a SecA-dependent process.

1988 • 58 citations

Leader peptidase

1991 • 53 citations

Secretion of methanol-insoluble heat-stable enterotoxin (STB): energy- and secA-dependent conversion of pre-STB to an intermediate indistinguishable from the extracellular toxin

1990 • 49 citations

Positive charges in the cytoplasmic domain of Escherichia coli leader peptidase prevent an apolar domain from functioning as a signal.

1989 • 48 citations

Membrane insertion of the Escherichia coli MalF protein in cells with impaired secretion machinery.

1991 • 47 citations

SecB-independent export of Escherichia coli ribose-binding protein (RBP): some comparisons with export of maltose-binding protein (MBP) and studies with RBP-MBP hybrid proteins

1990 • 40 citations

Accumulation of prolipoprotein in Escherichia coli mutants defective in protein secretion

1985 • 37 citations

Both a short hydrophobic domain and a carboxyl-terminal hydrophilic region are important for signal function in the Escherichia coli leader peptidase

1989 • 30 citations

In vitro translocation of secretory proteins possessing no charges at the mature domain takes place efficiently in a protonmotive force-dependent manner.

1992 • 29 citations

Export of honeybee prepromelittin in Escherichia coli depends on the membrane potential but does not depend on proteins secA and secY

1989 • 28 citations

Temperature-dependent insertion of prolipoprotein into Escherichia coli membrane vesicles and requirements for ATP, soluble factors, and functional SecY protein for the overall translocation process

1989 • 22 citations

A lower size limit exists for export of fragments of an outer membrane protein (OmpA) of Escherichia coli K-12

1989 • 21 citations