The gene encoding the biotin carboxylase subunit of Escherichia coli acetyl-CoA carboxylase.

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

Current protocols in molecular biology

1988 • 7,584 citations

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

1986 • 6,284 citations

Screening λgt Recombinant Clones by Hybridization to Single Plaques in Situ

1977 • 4,696 citations

Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid

1978 • 4,472 citations

Experiments With Gene Fusions

1984 • 2,487 citations

DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.

1987 • 2,266 citations

The physical map of the whole E. coli chromosome: Application of a new strategy for rapid analysis and sorting of a large genomic library

1987 • 1,768 citations

Experiments with gene fusions

1985 • 1,210 citations

Similar Amino Acid Sequences: Chance or Common Ancestry?

1981 • 855 citations

Single-stranded DNA ‘blue’ T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineering

1986 • 816 citations

Extent of N-terminal methionine excision from Escherichia coli proteins is governed by the side-chain length of the penultimate amino acid.

1989 • 744 citations

Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of Gram-negative bacteria

1987 • 700 citations

High-copy-number and low-copy-number plasmid vectors for lacZα-complementation and chloramphenicol- or kanamycin-resistance selection

1987 • 695 citations

Easy identification of cDNA clones.

1983 • 587 citations

THE MECHANISM OF BIOTIN-DEPENDENT ENZYMES

1989 • 446 citations

Hydrolysis of Proteins

1965 • 408 citations

Biotination of proteins in vivo. A post-translational modification to label, purify, and study proteins.

1990 • 402 citations

The codon preference plot: graphic analysis of protein coding sequences and prediction of gene expression

1984 • 381 citations

Effect of manganese ions on the incorporation of dideoxynucleotides by bacteriophage T7 DNA polymerase and Escherichia coli DNA polymerase I.

1989 • 292 citations

Evolutionary conservation among biotin enzymes.

1988 • 266 citations

Acetyl Coenzyme A Carboxylase System of Escherichia coli

1974 • 204 citations

The E. coli bio operon: Transcriptional repression by an essential protein modification enzyme

1989 • 175 citations

Structure of the coding sequence and primary amino acid sequence of acetyl-coenzyme A carboxylase.

1988 • 171 citations

Primary structure of chicken liver acetyl-CoA carboxylase deduced from cDNA sequence.

1988 • 115 citations

Mutations in the nucleotide binding loop of adenylate kinase of Escherichia coli

1988 • 114 citations

Sequence and domain structure of yeast pyruvate carboxylase.

1988 • 101 citations

Acetyl Coenzyme A Carboxylase System of Escherichia coli

1974 • 98 citations

The sodium ion translocating oxalacetate decarboxylase of Klebsiella pneumoniae. Sequence of the biotin-containing alpha-subunit and relationship to other biotin-containing enzymes.

1988 • 97 citations

Cloning, sequence, and expression of the pantothenate permease (panF) gene of Escherichia coli

1990 • 92 citations

Nucleotide sequence of the birA gene encoding the biotin operon represser and biotin holoenzyme synthetase functions of Escherichia coli

1985 • 92 citations

Acetyl CoA carboxylase

1971 • 91 citations

Conformational Flexibility and Folding of Synthetic Peptides Representing an Interdomain Segment of Polypeptide Chain in the Pyruvate Dehydrogenase Multienzyme Complex of Escherichia coli

1989 • 83 citations

Dissection of the functional domains of Escherichia coli carbamoyl phosphate synthetase by site-directed mutagenesis.

1990 • 76 citations

Expression of the biotin biosynthetic operon of Escherichia coli is regulated by the rate of protein biotination.

1988 • 76 citations

A rapid method to quantitate non-labeled RNA species in bacterial cells

1988 • 76 citations

Nucleotide sequence of thefabE gene and flanking regions containing a bent DNA sequence ofEscherichia coli

1989 • 75 citations

Temperature-Sensitive Mutants of Escherichia coli Requiring Saturated and Unsaturated Fatty Acids for Growth: Isolation and Properties

1972 • 70 citations

Identification of the btuCED polypeptides and evidence for their role in vitamin B12 transport in Escherichia coli

1986 • 70 citations

Acetyl Coenzyme A Carboxylase

1972 • 67 citations

2 Acyl-CoA Carboxylases

1972 • 65 citations

Structural analysis of the Escherichia coli K-12 hisT operon by using a kanamycin resistance cassette

1987 • 63 citations

Sequence Analysis, Biogenesis, and Mitochondrial Import of the α-Subunit of Rat Liver Propionyl-CoA Carboxylase

1989 • 61 citations

Random cloning of bent DNA segments fromEscherichia colichromosome and primary characterization of their structures

1987 • 61 citations

Escherichia coli exports previously folded and biotinated protein domains

1991 • 59 citations

Investigation of the mechanism of active site coupling in the pyruvate dehydrogenase multienzyme complex of Escherichia coli by protein engineering

1988 • 57 citations

Amino acid sequence of Escherichia coli biotin carboxyl carrier protein (9100).

1977 • 56 citations

Cloning and expression of the 1.3S biotin-containing subunit of transcarboxylase.

1985 • 55 citations

Coding sequence of the precursor of the beta subunit of rat propionyl-CoA carboxylase.

1986 • 54 citations

ATPase activity of biotin carboxylase provides evidence for initial activation of HCO3− by ATP in the carboxylation of biotin

1986 • 51 citations

Acetyl Coenzyme A Carboxylase

1973 • 50 citations

On the intermediacy of carboxyphosphate in biotin-dependent carboxylations

1988 • 48 citations

Use of avidin-sepharose to lsolate and identify biotin polypeptides from crude extracts

1978 • 44 citations

The subunit structure and function of the propionyl coenzyme A carboxylase of Mycobacterium smegmatis.

1982 • 40 citations

Catalytic mechanism of biotin carboxylase: steady-state kinetic investigations

1988 • 38 citations

Carbon-13 and deuterium isotope effects on the catalytic reactions of biotin carboxylase

1988 • 37 citations

Partial Characterization of a Temperature-Sensitive Mutation Affecting Acetyl Coenzyme A Carboxylase in Escherichia coli K-12

1976 • 24 citations

A Rapid Procedure for Cloning Genes from λ Libraries by Complementation ofE. coliDefective Mutants: Application to thefabERegion of theE. coliChromosome

1989 • 24 citations

Insertional restoration of β-galactosidase α-complementation (white-to-blue colony screening) facilitates assembly of synthetic genes

1988 • 19 citations

Screening recombinant clones containing sequences homologous to Escherichia coli genes using single-stranded bacteriophage vector

1986 • 14 citations

Size determination of multienzyme complexes using two‐dimensional agarose gel electrophoresis

1989 • 13 citations