Protein Translocation: Tunnel Vision

Intracellular protein topogenesis

1980 • 1,378 citations

Sec6l-mediated transfer of a membrane protein from the endoplasmic reticulum to the proteasome for destruction

1996 • 1,101 citations

Signal Sequence Recognition and Protein Targeting to the Endoplasmic Reticulum Membrane

1994 • 821 citations

Protein translocation into proteoliposomes reconstituted from purified components of the endoplasmic reticulum membrane

1993 • 654 citations

A protein-conducting channel in the endoplasmic reticulum

1991 • 613 citations

PROTEIN TRANSPORT ACROSS THE EUKARYOTIC ENDOPLASMIC RETICULUM AND BACTERIAL INNER MEMBRANES

1996 • 560 citations

Mutant analysis links the translocon and BiP to retrograde protein transport for ER degradation

1997 • 556 citations

ER Quality Control: The Cytoplasmic Connection

1997 • 528 citations

The purified E. coli integral membrane protein is sufficient for reconstitution of SecA-dependent precursor protein translocation

1990 • 516 citations

GENETIC ANALYSIS OF PROTEIN EXPORT IN ESCHERICHIA COLI K12

1985 • 432 citations

Sec61p mediates export of a misfolded secretory protein from the endoplasmic reticulum to the cytosol for degradation

1997 • 412 citations

Posttranslational protein transport in yeast reconstituted with a purified complex of Sec proteins and Kar2p

1995 • 400 citations

Assembly of yeast Sec proteins involved in translocation into the endoplasmic reticulum into a membrane-bound multisubunit complex

1991 • 359 citations

Oligomeric Rings of the Sec61p Complex Induced by Ligands Required for Protein Translocation

1996 • 346 citations

The E. coli Signal Recognition Particle Is Required for the Insertion of a Subset of Inner Membrane Proteins

1997 • 339 citations

Secretory proteins move through the endoplasmic reticulum membrane via an aqueous, gated pore

1994 • 338 citations

SecA membrane cycling at SecYEG is driven by distinct ATP binding and hydrolysis events and is regulated by SecD and SecF

1995 • 298 citations

GENETIC ANALYSIS OF PROTEIN EXPORT IN ESCHERICHIA COLI

1990 • 291 citations

A Sec63p-BiP complex from yeast is required for protein translocation in a reconstituted proteoliposome.

1993 • 290 citations

The signal sequence moves through a ribosomal tunnel into a noncytoplasmic aqueous environment at the ER membrane early in translocation

1993 • 286 citations

The Aqueous Pore through the Translocon Has a Diameter of 40–60 Å during Cotranslational Protein Translocation at the ER Membrane

1997 • 279 citations

A posttargeting signal sequence recognition event in the endoplasmic reticulum membrane

1995 • 278 citations

Both Lumenal and Cytosolic Gating of the Aqueous ER Translocon Pore Are Regulated from Inside the Ribosome during Membrane Protein Integration

1997 • 255 citations

The protein-conducting channel in the membrane of the endoplasmic reticulum is open laterally toward the lipid bilayer

1995 • 253 citations

The Cotranslational Integration of Membrane Proteins into the Phospholipid Bilayer Is a Multistep Process

1996 • 246 citations

Systematic probing of the environment of a translocating secretory protein during translocation through the ER membrane.

1994 • 237 citations

A signal sequence is not required for protein export in prlA mutants of Escherichia coli.

1993 • 219 citations

Signal peptides open protein-conducting channels in E. coli

1992 • 216 citations

Reconstitution of a protein translocation system containing purified SecY, SecE, and SecA from Escherichia coli.

1991 • 212 citations

Anionic phospholipids are determinants of membrane protein topology

1997 • 210 citations

The SecDFyajC domain of preprotein translocase controls preprotein movement by regulating SecA membrane cycling

1997 • 201 citations

Molecular Mechanism of Membrane Protein Integration into the Endoplasmic Reticulum

1997 • 191 citations

Signal sequence-dependent function of the TRAM protein during early phases of protein transport across the endoplasmic reticulum membrane.

1996 • 182 citations

Binding of Secretory Precursor Polypeptides to a Translocon Subcomplex Is Regulated by BiP

1997 • 161 citations

SecA protein is exposed to the periplasmic surface of the E. coli inner membrane in its active state

1994 • 158 citations

Evidence for the loop model of signal-sequence insertion into the endoplasmic reticulum.

1988 • 148 citations

Membrane protein topology: effects of delta mu H+ on the translocation of charged residues explain the ‘positive inside’ rule.

1994 • 148 citations

Protein Transport by Purified Yeast Sec Complex and Kar2p Without Membranes

1997 • 146 citations

Multiple Determinants Direct the Orientation of Signal–Anchor Proteins: The Topogenic Role of the Hydrophobic Signal Domain

1997 • 145 citations

Genetic interactions between KAR2 and SEC63, encoding eukaryotic homologues of DnaK and DnaJ in the endoplasmic reticulum.

1993 • 135 citations

Mechanism of Polypeptide Translocation into the Endoplasmic Reticulum

1996 • 128 citations

Biogenesis of Polytopic Membrane Proteins: Membrane Segments Assemble within Translocation Channels prior to Membrane Integration

1996 • 123 citations

Escherichia coli Preprotein Translocase

1996 • 108 citations

Membrane insertion and assembly of ductin: a polytopic channel with dual orientations.

1995 • 96 citations

On the transfer of integral proteins into membranes.

1987 • 92 citations

Sequence-Specific Alteration of the Ribosome–Membrane Junction Exposes Nascent Secretory Proteins to the Cytosol

1996 • 90 citations

PrlA and PrlG suppressors reduce the requirement for signal sequence recognition

1994 • 77 citations

The Sec61 complex is essential for the insertion of proteins into the membrane of the endoplasmic reticulum

1995 • 63 citations

Directionality in protein translocation across membranes: the N-tail phenomenon

1995 • 51 citations

Snapshots of membrane-translocating proteins

1996 • 46 citations

Purified Escherichia coli preprotein translocase catalyzes multiple cycles of precursor protein translocation

1993 • 42 citations

Stage- and ribosome-specific alterations in nascent chain-Sec61p interactions accompany translocation across the ER membrane.

1995 • 34 citations

Protein translocation genetics

1995 • 14 citations