Protein Targeting to the Bacterial Cytoplasmic Membrane

The Complete Genome Sequence of Escherichia coli K-12

1997 • 7,325 citations

Whole-Genome Random Sequencing and Assembly of Haemophilus influenzae Rd

1995 • 5,571 citations

The GTPase superfamily: conserved structure and molecular mechanism

1991 • 3,365 citations

Signal sequences

1985 • 2,179 citations

The complete general secretory pathway in gram-negative bacteria

1993 • 1,668 citations

The signal peptide

1990 • 1,096 citations

Signal Sequence Recognition and Protein Targeting to the Endoplasmic Reticulum Membrane

1994 • 821 citations

Signal recognition particle contains a 7S RNA essential for protein translocation across the endoplasmic reticulum

1982 • 766 citations

How signal sequences maintain cleavage specificity

1984 • 725 citations

A common export pathway for proteins binding complex redox cofactors?

1996 • 661 citations

The binding cascade of SecB to SecA to SecYE mediates preprotein targeting to the E. coli plasma membrane

1990 • 561 citations

PROTEIN TRANSPORT ACROSS THE EUKARYOTIC ENDOPLASMIC RETICULUM AND BACTERIAL INNER MEMBRANES

1996 • 560 citations

The ATPase activity of secA is regulated by acidic phospholipids, secY, and the leader and mature domains of precursor proteins

1990 • 551 citations

SecA promotes preprotein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertion

1994 • 529 citations

Overlapping functions of components of a bacterial Sec-independent protein export pathway

1998 • 525 citations

The purified E. coli integral membrane protein is sufficient for reconstitution of SecA-dependent precursor protein translocation

1990 • 516 citations

Purification of a membrane-associated protein complex required for protein translocation across the endoplasmic reticulum.

1980 • 502 citations

Model for signal sequence recognition from amino-acid sequence of 54K subunit of signal recognition particle

1989 • 474 citations

Correlation of competence for export with lack of tertiary structure of the mature species: A study in vivo of maltose-binding protein in E. coli

1986 • 473 citations

A Novel and Ubiquitous System for Membrane Targeting and Secretion of Cofactor-Containing Proteins

1998 • 465 citations

ΔμH+ and ATP function at different steps of the catalytic cycle of preprotein translocase

1991 • 456 citations

Signal sequences specify the targeting route to the endoplasmic reticulum membrane.

1996 • 455 citations

GENETIC ANALYSIS OF PROTEIN EXPORT IN ESCHERICHIA COLI K12

1985 • 432 citations

Supervising the fold: functional principles of molecular chaperones

1996 • 420 citations

Analysis of theEscherichia coligenome. V. DNA sequence of the region from 76.0 to 81.5 minutes

1994 • 387 citations

A novel Sec-independent periplasmic protein translocation pathway in Escherichia coli

1998 • 372 citations

Homology of 54K protein of signal-recognition particle, docking protein and two E. coli proteins with putative GTP–binding domains

1989 • 357 citations

The E. coli Signal Recognition Particle Is Required for the Insertion of a Subset of Inner Membrane Proteins

1997 • 339 citations

Photocrosslinking of the signal sequence of nascent preprolactin to the 54-kilodalton polypeptide of the signal recognition particle.

1986 • 337 citations

The signal sequence of nascent preprolactin interacts with the 54K polypeptide of the signal recognition particle

1986 • 336 citations

The antifolding activity of SecB promotes the export of the E. coli maltose-binding protein

1988 • 326 citations

Three pure chaperone proteins of Escherichia coli--SecB, trigger factor and GroEL--form soluble complexes with precursor proteins in vitro.

1989 • 317 citations

SecA membrane cycling at SecYEG is driven by distinct ATP binding and hydrolysis events and is regulated by SecD and SecF

1995 • 298 citations

The Escherichia coli SRP and SecB targeting pathways converge at the translocon

1998 • 292 citations

GENETIC ANALYSIS OF PROTEIN EXPORT IN ESCHERICHIA COLI

1990 • 291 citations

An E. coli Ribonucleoprotein Containing 4.5 S RNA Resembles Mammalian Signal Recognition Particle

1990 • 285 citations

Distinct catalytic roles of the SecYE, SecG and SecDFyajC subunits of preprotein translocase holoenzyme

1997 • 285 citations

Early events in preprotein recognition in E. coli: interaction of SRP and trigger factor with nascent polypeptides.

1995 • 276 citations

Sec-Independent Protein Translocation by the Maize Hcf106 Protein

1997 • 275 citations

Protein-specific energy requirements for protein transport across or into thylakoid membranes. Two lumenal proteins are transported in the absence of ATP.

1992 • 275 citations

Evidence for specificity at an early step in protein export in Escherichia coli

1985 • 272 citations

Mutations in a new gene, secB, cause defective protein localization in Escherichia coli

1983 • 270 citations

The E. coli ffh gene is necessary for viability and efficient protein export

1992 • 268 citations

A new type of signal peptide: central role of a twin-arginine motif in transfer signals for the delta pH-dependent thylakoidal protein translocase.

1995 • 266 citations

The chemistry and enzymology of the type I signal peptidases

1997 • 262 citations

Differential expression of hydrogenase isoenzymes in Escherichia coli K-12: evidence for a third isoenzyme

1985 • 260 citations

The methionine-rich domain of the 54 kd protein subunit of the signal recognition particle contains an RNA binding site and can be crosslinked to a signal sequence.

1990 • 256 citations

An alternative protein targeting pathway in Escherichia coli: studies on the role of FtsY.

1994 • 252 citations

Effects of mutations in heat-shock genes groES and groEL on protein export in Escherichia coli.

1989 • 250 citations

Modulation of Folding Pathways of Exported Proteins by the Leader Sequence

1988 • 243 citations

Purified secB protein of Escherichia coli retards folding and promotes membrane translocation of the maltose-binding protein in vitro.

1988 • 243 citations

Oxa1p, an essential component of the N-tail protein export machinery in mitochondria

1998 • 241 citations

Signal peptides: exquisitely designed transport promoters

1994 • 231 citations

SecA interacts with secretory proteins by recognizing the positive charge at the amino terminus of the signal peptide in Escherichia coli.

1990 • 229 citations

Structure of the conserved GTPase domain of the signal recognition particle

1997 • 229 citations

Molecular characterization of an operon (hyp) necessary for the activity of the three hydrogenase isoenzymes in Escherichia coli

1991 • 226 citations

A Kinetic Partitioning Model of Selective Binding of Nonnative Proteins by the Bacterial Chaperone SecB

1991 • 225 citations

Requirement of GTP Hydrolysis for Dissociation of the Signal Recognition Particle from Its Receptor

1991 • 222 citations

A signal sequence is not required for protein export in prlA mutants of Escherichia coli.

1993 • 219 citations

Interaction of E. coli Ffh/4.5S ribonucleoprotein and FtsY mimics that of mammalian signal recognition particle and its receptor

1994 • 218 citations

Effects of two sec genes on protein assembly into the plasma membrane of Escherichia coli.

1985 • 217 citations

DnaK and DnaJ heat shock proteins participate in protein export in Escherichia coli.

1992 • 214 citations

E. coli 4.5S RNA is part of a ribonucleoprotein particle that has properties related to signal recognition particle

1990 • 212 citations

Signal peptidases in prokaryotes and eukaryotes - a new protease family

1992 • 212 citations

OXA1, a Saccharomyces cerevisiae Nuclear Gene whose Sequence is Conserved form Prokaryotes to Eukaryotes Controls Cytochrome Oxidase Biogenesis

1994 • 211 citations

Crystal structure of the NG domain from the signal-recognition particle receptor FtsY

1997 • 210 citations

Different exported proteins in E. coli show differences in the temporal mode of processing in vivo

1981 • 203 citations

A proton gradient is required for the transport of two lumenal oxygen-evolving proteins across the thylakoid membrane

1991 • 195 citations

The molecular chaperone SecB is released from the carboxy-terminus of SecA during initiation of precursor protein translocation

1997 • 193 citations

The signal recognition particle receptor is a complex that contains two distinct polypeptide chains.

1986 • 189 citations

Co-translational protein targeting catalyzed by the Escherichia coli signal recognition particle and its receptor

1997 • 187 citations

Signal-sequence recognition by an Escherichia coli ribonucleoprotein complex

1992 • 184 citations

GTP binding and hydrolysis by the signal recognition particle during initiation of protein translocation

1993 • 183 citations

SRP-RNA sequence alignment and secondary structure

1991 • 181 citations

Human SRP RNA and E. coli 4.5S RNA contain a highly homologous structural domain

1988 • 178 citations

Nascent membrane and presecretory proteins synthesized in Escherichia coli associate with signal recognition particle and trigger factor

1997 • 175 citations

Heat-shock proteins DnaK and GroEL facilitate export of LacZ hybrid proteins in E. coli

1990 • 175 citations

Thesec andprl genes ofEscherichia coli

1990 • 174 citations

A folded protein can be transported across the chloroplast envelope and thylakoid membranes.

1997 • 173 citations

The 54-kD protein of signal recognition particle contains a methionine-rich RNA binding domain.

1990 • 170 citations

Conformational behavior of Escherichia coli OmpA signal peptides in membrane mimetic environments

1993 • 169 citations

The Sec system

1998 • 164 citations

Alteration of the amino terminus of the mature sequence of a periplasmic protein can severely affect protein export in Escherichia coli.

1988 • 163 citations

Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2

1994 • 159 citations

Assembly of a cytoplasmic membrane protein in Escherichia coli is dependent on the signal recognition particle

1996 • 156 citations

FtsY, the Prokaryotic Signal Recognition Particle Receptor Homologue, Is Essential for Biogenesis of Membrane Proteins

1997 • 155 citations

Two nuclear mutations disrupt distinct pathways for targeting proteins to the chloroplast thylakoid.

1995 • 154 citations

Pathway specificity for a ΔpH-dependent precursor thylakoid lumen protein is governed by a 'Sec-avoidance' motif in the transfer peptide and a 'Sec-incompatible' mature protein

1997 • 153 citations

Biogenesis of the Gram-Negative Bacterial Envelope

1997 • 152 citations

Membrane protein topology: effects of delta mu H+ on the translocation of charged residues explain the ‘positive inside’ rule.

1994 • 148 citations

The C Terminus of SecA Is Involved in Both Lipid Binding and SecB Binding

1995 • 147 citations

Molecular chaperones and protein translocation across the Escherichia coli inner membrane

1991 • 143 citations

The general secretory pathway in bacteria

1993 • 141 citations

Topology of signal recognition particle receptor in endoplasmic reticulum membrane

1985 • 140 citations

Cloning and sequencing of a putative Escherichia coli [NiFe] hydrogenase-1 operon containing six open reading frames

1990 • 140 citations

PET1402, a nuclear gene required for proteolytic processing of cytochrome oxidase subunit 2 in yeast

1994 • 135 citations

High selectivity with low specificity: how SecB has solved the paradox of chaperone binding

1995 • 134 citations

Catalysis of Amide Proton Exchange by the Molecular Chaperones GroEL and SecB

1996 • 134 citations

SecYEG and SecA Are the Stoichiometric Components of Preprotein Translocase

1995 • 125 citations

Reconstitution of an efficient protein translocation machinery comprising SecA and the three membrane proteins, SecY, SecE, and SecG (p12).

1994 • 125 citations

Escherichia coli SecB protein associates with exported protein precursors in vivo.

1989 • 123 citations

Effects of Escherichia coli secB mutations on pre-maltose binding protein conformation and export kinetics.

1988 • 117 citations

ProOmpA contains secondary and tertiary structure prior to translocation and is shielded from aggregation by association with SecB protein.

1990 • 114 citations

Preprotein transfer to the Escherichia coli translocase requires the co‐operative binding of SecB and the signal sequence to SecA

1998 • 114 citations

The Functional Integration of a Polytopic Membrane Protein of Escherichia coli is Dependent on the Bacterial Signal‐Recognition Particle

1995 • 111 citations

Functional substitution of the signal recognition particle 54-kDa subunit by its Escherichia coli homolog.

1993 • 109 citations

SecB functions as a cytosolic signal recognition factor for protein export in E. coli

1989 • 107 citations

Specific recognition of the leader region of precursor proteins is required for the activation of translocation ATPase of Escherichia coli.

1989 • 107 citations

Topology of the Integral Membrane Form of Escherichia coli SecA Protein Reveals Multiple Periplasmically Exposed Regions and Modulation by ATP Binding

1997 • 107 citations

Highly selective binding of nascent polypeptides by an Escherichia coli chaperone protein in vivo

1993 • 107 citations

The affinity of signal recognition particle for presecretory proteins is dependent on nascent chain length.

1988 • 106 citations

Introduction of basic amino acid residues after the signal peptide inhibits protein translocation across the cytoplasmic membrane of Escherichia coli. Relation to the orientation of membrane proteins.

1988 • 100 citations

Novel secA alleles improve export of maltose-binding protein synthesized with a defective signal peptide

1989 • 99 citations

Protein Translocation into Peroxisomes

1996 • 97 citations

Conformational States Bound by the Molecular Chaperones GroEL and SecB: A Hidden Unfolding (Annealing) Activity

1996 • 97 citations

PrlA4 prevents the rejection of signal sequence defective preproteins by stabilizing the SecA-SecY interaction during the initiation of translocation

1998 • 96 citations

Signal peptide mutants ofEscherichia coli

1990 • 94 citations

Polymeric sequences reveal a functional interrelationship between hydrophobicity and length of signal peptides.

1990 • 93 citations

Mammalian and Escherichia coli signal recognition particles

1994 • 91 citations

Genetic studies on the inability of beta-galactosidase to be translocated across the Escherichia coli cytoplasmic membrane

1989 • 90 citations

Two distinct mechanisms for the translocation of proteins across the thylakoid membrane, one requiring the presence of a stromal protein factor and nucleotide triphosphates.

1994 • 85 citations

Targeting Determinants and Proposed Evolutionary Basis for the Sec and the Delta pH Protein Transport Systems in Chloroplast Thylakoid Membranes

1997 • 85 citations

Cytosolic factor purified from Escherichia coli is necessary and sufficient for the export of a preprotein and is a homotetramer of SecB.

1989 • 83 citations

Accumulation of secretory protein precursors in Escherichia coli induces the heat shock response

1993 • 83 citations

Site-directed mutagenesis of the hydrogenase signal peptide consensus box prevents export of a -lactamase fusion protein

1992 • 82 citations

The signal recognition particle receptor of Escherichia coli (FtsY) has a nucleotide exchange factor built into the GTPase domain

1997 • 80 citations

The NG domain of the prokaryotic signal recognition particle receptor, FtsY, is fully functional when fused to an unrelated integral membrane polypeptide

1997 • 79 citations

Analysis of mutational alterations in the hydrophilic segment of the maltose-binding protein signal peptide

1989 • 79 citations

GTPase domain of the 54-kD subunit of the mammalian signal recognition particle is required for protein translocation but not for signal sequence binding.

1993 • 78 citations

Domain Interactions in E. coli SRP: Stabilization of M Domain by RNA Is Required for Effective Signal Sequence Modulation of NG Domain

1997 • 78 citations

Differences between lumen targeting domains of chloroplast transit peptides determine pathway specificity for thylakoid transport.

1994 • 78 citations

PrlA and PrlG suppressors reduce the requirement for signal sequence recognition

1994 • 77 citations

Membrane association of FtsY, the E. coli SRP receptor

1997 • 77 citations

The synthesis of the two S-adenosyl-methionine synthetases is differently regulated in Saccharomyces cerevisiae

1991 • 76 citations

A Monomeric, Tightly Folded Stromal Intermediate on the △pH-dependent Thylakoidal Protein Transport Pathway

1995 • 76 citations

Import of barley photosystem I subunit N into the thylakoid lumen is mediated by a bipartite presequence lacking an intermediate processing site. Role of the delta pH in translocation across the thylakoid membrane.

1994 • 75 citations

Heat-shock proteins can substitute for SecB function during protein export in Escherichia coli.

1991 • 73 citations

Chloroplast SRP54 Interacts with a Specific Subset of Thylakoid Precursor Proteins

1997 • 71 citations

[1] Genetic analysis of protein export in Escherichia coli

1983 • 70 citations

Characterization of the Escherichia coli protein-export gene secB

1989 • 69 citations

The Escherichia coli trigger factor

1996 • 69 citations

Common structural features between eukaryotic 7SL RNAs, eubacterial 4.5S RNA and scRNA and archaebacterial 7S RNA

1988 • 69 citations

Binding of SecB to ribosome-bound polypeptides has the same characteristics as binding to full-length, denatured proteins

1997 • 68 citations

Isolation and characterization of mutant strains of Escherichia coli altered in H2 metabolism

1985 • 66 citations

Membrane-bound conformation of a signal peptide: A transferred nuclear Overhauser effect analysis

1993 • 65 citations

Electrospray mass spectrometric investigation of the chaperone SecB

1996 • 65 citations

Targeting of signal sequenceless proteins for export in Escherichia coli with altered protein translocase.

1996 • 64 citations

Determination of the binding frame within a physiological ligand for the chaperone SecB

1994 • 63 citations

Diffusion-Limited Interaction between Unfolded Polypeptides and the Escherichia coli Chaperone SecB

1995 • 62 citations

OmpF-Lpp signal sequence mutants with varying charge hydrophobicity ratios provide evidence for a phosphatidylglycerol-signal sequence interaction during protein translocation across the Escherichia coli inner membrane

1993 • 61 citations

Suppression of signal sequence defects and azide resistance in Escherichia coli commonly result from the same mutations in secA

1995 • 60 citations

Lipid involvement in protein translocation in Escherichia coli

1990 • 59 citations

PhoE Signal Peptide Inserts into Micelles as a Dynamic Helix-Break-Helix Structure, Which Is Modulated by the Environment. A Two-Dimensional 1H NMR Study

1995 • 58 citations

Titration of protein transport activity by incremental changes in signal peptide hydrophobicity

1993 • 58 citations

Export of maltose-binding protein species with altered charge distribution surrounding the signal peptide hydrophobic core in Escherichia coli cells harboring prl suppressor mutations

1992 • 57 citations

Targeting of GroEL to SecA on the cytoplasmic membrane of Escherichia coli

1998 • 57 citations

A Signal Peptide That Directs Non-Sec Transport in Bacteria Also Directs Efficient and Exclusive Transport on the Thylakoid Delta pH Pathway

1998 • 56 citations

Expression of gpsA encoding biosynthetic sn‐glycerol 3‐phosphate dehydrogenase suppresses both the LB⁻ phenotype of a secB null mutant and the cold‐sensitive phenotype of a secG null mutant

1997 • 55 citations

Export incompatibility of N-terminal basic residues in a mature polypeptide of Eschericbia coli can be alleviated by optimising the signal peptide

1990 • 55 citations

The requirement of a positive charge at the amino terminus can be compensated for by a longer central hydrophobic stretch in the functioning of signal peptides.

1992 • 54 citations

Identification of a soluble SecA/SecB complex by means of a subfractionated cell-free export system.

1994 • 54 citations

Mapping of the Binding Frame for the Chaperone SecB within a Natural Ligand, Galactose-binding Protein

1995 • 53 citations

Growing up in a dangerous environment: a network of multiple targeting and folding pathways for nascent polypeptides in the cytosol

1996 • 52 citations

Directionality in protein translocation across membranes: the N-tail phenomenon

1995 • 51 citations

Synthetic Signal Peptides Specifically Recognize SecA and Stimulate ATPase Activity in the Absence of Preprotein

1998 • 51 citations

Requirements for the Translocation of Elongation-arrested, Ribosome-associated OmpA across the Plasma Membrane ofEscherichia coli

1998 • 50 citations

A complex of the signal sequence binding protein and the SRP RNA promotes translocation of nascent proteins.

1995 • 49 citations

A Mutation in the Escherichia coli secY Gene That Produces Distinct Effects on Inner Membrane Protein Insertion and Protein Export

1998 • 49 citations

Successive action of Escherichia coli chaperones in vivo

1994 • 49 citations

Interaction of SecB with soluble SecA¹

1997 • 49 citations

Diverse Effects of Mutation on the Activity of the Escherichia coli Export Chaperone SecB

1995 • 48 citations

The functioning of the SRP receptor FtsY in protein‐targeting in E. coli is correlated with its ability to bind and hydrolyse GTP

1995 • 47 citations

The presence of both the signal sequence and a region of mature LamB protein is required for the interaction of LamB with the export factor SecB.

1990 • 42 citations

prl Mutations in the Escherichia coli secG Gene

1997 • 42 citations

Chaperone SecB from Escherichia coli Mediates Kinetic Partitioning via a Dynamic Equilibrium with Its Ligands

1997 • 42 citations

Calorimetric analyses of the interaction between SecB and its ligands

1998 • 41 citations

High-affinity binding of Escherichia coli SecB to the signal sequence region of a presecretory protein.

1995 • 39 citations

In‐vitro studies on the folding characteristics of the Escherichia coli precursor protein prePhoE

1992 • 38 citations

Sequence analysis of a DNA fragment from Buchnera aphidicola (an endosymbiont of aphids) containing genes homologous to dnaG, rpoD, cysE, and secB

1992 • 35 citations

Kinetic Partitioning

1997 • 34 citations

Formation of SRP‐like particle induces a conformational change in E. coli 4.5S RNA

1994 • 32 citations

Mutations of the molecular chaperone protein SecB which alter the interaction between SecB and maltose-binding protein.

1993 • 32 citations

SecB‐binding does not maintain the translocation‐competent state of prePhoE

1992 • 31 citations

The △pH-driven, ATP-independent Protein Translocation Mechanism in the Chloroplast Thylakoid Membrane

1995 • 31 citations

Carboxy-Terminal Region of Escherichia coli SecA ATPase Is Important to Promote Its Protein Translocation Activity in Vivo

1994 • 29 citations

Characterization of a region in mature LamB protein that interacts with a component of the export machinery of Escherichia coli.

1990 • 27 citations

Requirement of the SecB chaperone for export of a non-secretory polypeptide in Escherichia coli

1991 • 27 citations

Determination of the binding frame of the chaperone SecB within the physiological ligand oligopeptide‐binding protein

1997 • 25 citations

GroEL Binds to and Unfolds Rhodanese Posttranslationally

1996 • 23 citations

Influence of impaired chaperone or secretion function on SecB production in Escherichia coli

1996 • 23 citations

Requirement for conformational flexibility in the signal sequence of precursor protein.

1994 • 23 citations

Binding of an N-terminal Rhodanese Peptide to DnaJ and to Ribosomes

1996 • 22 citations

Folding of barnase in the presence of the molecular chaperone SecB

1997 • 22 citations

Folding in vitro and transport in vivo of pre-beta-lactamase are SecB independent.

1991 • 22 citations

Folding in vitro and transport in vivo of pre‐β‐lactamase are SecB independent

1991 • 22 citations

Carbon source-dependent synthesis of SecB, a cytosolic chaperone involved in protein translocation across Escherichia coli membranes

1997 • 21 citations

Identification of a Sequence Motif That Confers SecB Dependence on a SecB-Independent Secretory Protein In Vivo

1998 • 20 citations

Escherichia coli SecB stimulates export without maintaining export competence of ribose-binding protein signal sequence mutants

1996 • 20 citations

Unusual Characteristics of Amino‐Terminal and Hydrophobic Domains in Nuclear‐Encoded Thylakoid Signal Peptides

1997 • 19 citations

??H⁺dependency of in vitro protein translocation into Escherichia coli inner-membrane vesicles varies with the signal-sequence core-region composition

1996 • 18 citations

Chaperone SecB: Conformational changes demonstrated by circular dichroism

1995 • 15 citations

SecA, a novel ATPase that converts chemical energy into a mechanical force to drive precursor protein translocation

1995 • 13 citations

Export of proteins across membranes: The helix reversion hypothesis

1989 • 11 citations

Substitution of Fifty Four Homologue (Ffh) in Escherichia coli with the Mammalian 54–kDa Protein of Signal‐Recognition Particle

1996 • 11 citations

Translocation of proteins across the bacterial cytoplasmic membrane

1996 • 10 citations

PrlA4 prevents the rejection of signal sequence defective preproteins by stabilizing the SecA-SecY interaction during the initiation of translocation (vol 17, pg 3631, 1998)

1998 • 2 citations