Positively charged amino acids placed next to a signal sequence block protein translocation more efficiently in Escherichia coli than in mammalian microsomes

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

Signal sequences

1985 • 2,179 citations

Solid‐Phase Peptide Synthesis

1969 • 624 citations

Context Effects and Inefficient Initiation at Non-AUG Codons in Eucaryotic Cell-Free Translation Systems

1989 • 471 citations

Context effects and inefficient initiation at non-AUG codons in eucaryotic cell-free translation systems.

1989 • 272 citations

Species‐specific variation in signal peptide design Implications for protein secretion in foreign hosts

1989 • 254 citations

Analysis of the distribution of charged residues in the N-terminal region of signal sequences: implications for protein export in prokaryotic and eukaryotic cells.

1984 • 230 citations

Internally located cleavable signal sequences direct the formation of Semliki Forest virus membrane proteins from a polyprotein precursor

1991 • 223 citations

The role of charged amino acids in the localization of secreted and membrane proteins

1990 • 219 citations

Effects of two sec genes on protein assembly into the plasma membrane of Escherichia coli.

1985 • 217 citations

The isolation of homogeneous leader peptidase from a strain of Escherichia coli which overproduces the enzyme.

1982 • 179 citations

Net N-C charge imbalance may be important for signal sequence function in bacteria

1986 • 176 citations

Alteration of the amino terminus of the mature sequence of a periplasmic protein can severely affect protein export in Escherichia coli.

1988 • 163 citations

Negatively charged phospholipids restore prePhoE translocation across phosphatidylglycerol-depleted Escherichia coli inner membranes

1991 • 159 citations

Positively charged residues are important determinants of membrane protein topology

1990 • 132 citations

High-level expression of M13 gene II protein from an inducible polycistronic messenger RNA

1985 • 130 citations

Positively charged amino acid residues can act as topogenic determinants in membrane proteins.

1989 • 122 citations

Solid-Phase Peptide Synthesis

1976 • 117 citations

Mechanisms of membrane assembly: general lessons from the study of M13 coat protein and Escherichia coli leader peptidase

1988 • 100 citations

Introduction of basic amino acid residues after the signal peptide inhibits protein translocation across the cytoplasmic membrane of Escherichia coli. Relation to the orientation of membrane proteins.

1988 • 100 citations

A 30-residue-long "export initiation domain" adjacent to the signal sequence is critical for protein translocation across the inner membrane of Escherichia coli.

1991 • 95 citations

A small hydrophobic domain anchors leader peptidase to the cytoplasmic membrane of Escherichia coli.

1987 • 90 citations

Bacterial leader peptidase, a membrane protein without a leader peptide, uses the same export pathway as pre-secretory proteins

1984 • 84 citations

Leader Peptidase of Escherichia coli : Critical Role of a Small Domain in Membrane Assembly

1987 • 72 citations

A conservative amino acid substitution, arginine for lysine, abolishes export of a hybrid protein in Escherichia coli

1989 • 69 citations

The role of the polar, carboxyl-terminal domain of Escherichia coli leader peptidase in its translocation across the plasma membrane.

1986 • 68 citations

The cytoplasmic domain of Escherichia coli leader peptidase is a "translocation poison" sequence.

1988 • 61 citations

Export incompatibility of N-terminal basic residues in a mature polypeptide of Eschericbia coli can be alleviated by optimising the signal peptide

1990 • 55 citations

Positive charges in the cytoplasmic domain of Escherichia coli leader peptidase prevent an apolar domain from functioning as a signal.

1989 • 48 citations

The internal signal sequence of Escherichia coli leader peptidase is necessary, but not sufficient, for its rapid membrane assembly.

1987 • 47 citations

Both a short hydrophobic domain and a carboxyl-terminal hydrophilic region are important for signal function in the Escherichia coli leader peptidase

1989 • 30 citations

Characterization of the internal signal-anchor domain of Escherichia coli leader peptidase.

1988 • 21 citations

Alteration of N-terminal residues of mature human lysozyme affects its secretion in yeast and translocation into canine microsomal vesicles.

1991 • 17 citations

Deleted Work

1955 • 0 citations