The SecDFyajC domain of preprotein translocase controls preprotein movement by regulating SecA membrane cycling

Protein thiolation and reversible protein-protein conjugation. N-Succinimidyl 3-(2-pyridyldithio)propionate, a new heterobifunctional reagent

1978 • 1,226 citations

The ATPase activity of secA is regulated by acidic phospholipids, secY, and the leader and mature domains of precursor proteins

1990 • 551 citations

SecA promotes preprotein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertion

1994 • 529 citations

The purified E. coli integral membrane protein is sufficient for reconstitution of SecA-dependent precursor protein translocation

1990 • 516 citations

ΔμH+ and ATP function at different steps of the catalytic cycle of preprotein translocase

1991 • 456 citations

GENETIC ANALYSIS OF PROTEIN EXPORT IN ESCHERICHIA COLI K12

1985 • 432 citations

SecA protein hydrolyzes ATP and is an essential component of the protein translocation ATPase of Escherichia coli.

1989 • 406 citations

SecA membrane cycling at SecYEG is driven by distinct ATP binding and hydrolysis events and is regulated by SecD and SecF

1995 • 298 citations

Distinct catalytic roles of the SecYE, SecG and SecDFyajC subunits of preprotein translocase holoenzyme

1997 • 285 citations

Purified secB protein of Escherichia coli retards folding and promotes membrane translocation of the maltose-binding protein in vitro.

1988 • 243 citations

SecD and SecF facilitate protein export in Escherichia coli.

1994 • 215 citations

Reconstitution of a protein translocation system containing purified SecY, SecE, and SecA from Escherichia coli.

1991 • 212 citations

Inversion of the Membrane Topology of SecG Coupled with SecA-Dependent Preprotein Translocation

1996 • 205 citations

SecD is involved in the release of translocated secretory proteins from the cytoplasmic membrane of Escherichia coli.

1993 • 190 citations

The SecA and SecY subunits of translocase are the nearest neighbors of a translocating preprotein, shielding it from phospholipids.

1993 • 176 citations

ATP is essential for protein translocation into Escherichia coli membrane vesicles.

1985 • 163 citations

SecA protein is exposed to the periplasmic surface of the E. coli inner membrane in its active state

1994 • 158 citations

Both ATP and the electrochemical potential are required for optimal assembly of pro-OmpA into Escherichia coli inner membrane vesicles.

1986 • 142 citations

SecA, the peripheral subunit of the Escherichia coli precursor protein translocase, is functional as a dimer

1993 • 139 citations

ProOmpA spontaneously folds in a membrane assembly competent state which trigger factor stabilizes.

1988 • 135 citations

Stepwise Movement of Preproteins in the Process of Translocation across the Cytoplasmic Membrane of Escherichia coli

1995 • 126 citations

SecYEG and SecA Are the Stoichiometric Components of Preprotein Translocase

1995 • 125 citations

Reconstitution of an efficient protein translocation machinery comprising SecA and the three membrane proteins, SecY, SecE, and SecG (p12).

1994 • 125 citations

Assembly of translocation-competent proteoliposomes from detergent-solubilized rough microsomes

1990 • 118 citations

PrlA suppressor mutations cluster in regions corresponding to three distinct topological domains.

1993 • 115 citations

Precursor protein translocation by the Escherichia coli translocase is directed by the protonmotive force.

1992 • 113 citations

SecY, SecE, and band 1 form the membrane-embedded domain of Escherichia coli preprotein translocase.

1992 • 113 citations

SecD and SecF are required for the proton electrochemical gradient stimulation of preprotein translocation.

1994 • 111 citations

Escherichia coli Preprotein Translocase

1996 • 108 citations

In vitro Analysis of the Process of Translocation of OmpA across the Escherichia coli Cytoplasmic Membrane

1989 • 103 citations

Genetic and molecular characterization of the Escherichia coli secD operon and its products

1994 • 93 citations

A high Concentration of SecA Allows Proton Motive Force-independent Translocation of a Model Secretory Protein into Escherichia coli Membrane Vesicles

1989 • 92 citations

The proton motive force lowers the level of ATP required for the in vitro translocation of a secretory protein in Escherichia coli.

1990 • 85 citations

A Significant Fraction of Functional SecA Is Permanently Embedded in the Membrane

1996 • 85 citations

Expression of the pspA gene stimulates efficient protein export in Escherichia coli

1993 • 81 citations

Both an N-terminal 65-kDa domain and a C-terminal 30-kDa domain of SecA cycle into the membrane at SecYEG during translocation

1997 • 80 citations

The protease-protected 30 kDa domain of SecA is largely inaccessible to the membrane lipid phase

1997 • 69 citations

Subunit dynamics in Escherichia coli preprotein translocase.

1994 • 61 citations

Separable ATPase and Membrane Insertion Domains of the SecA Subunit of Preprotein Translocase

1996 • 54 citations

Purified Escherichia coli preprotein translocase catalyzes multiple cycles of precursor protein translocation

1993 • 42 citations

Band 1 subunit of Escherichia coli preportein translocase and integral membrane export factor P12 are the same protein.

1994 • 41 citations

Short Hydrophobic Segments in the Mature Domain of ProOmpA Determine Its Stepwise Movement during Translocation across the Cytoplasmic Membrane of Escherichia coli

1997 • 38 citations

Membrane vesicles containing overproduced SecY and SecE exhibit high translocation ATPase activity and countermovement of protons in a SecA- and presecretory protein-dependent manner.

1993 • 37 citations

Both an N-terminal 65-kDa domain and a C-terminal 30-kDa domain of SecA cycle into the membrane at SecYEG during translocation

1997 • 35 citations

Preprotein translocation creates a halide anion permeability in the Escherichia coli plasma membrane.

1992 • 29 citations

Genetic Analysis Of Protein Export In Escherichia Coli

1990 • 18 citations