Three pure chaperone proteins of Escherichia coli--SecB, trigger factor and GroEL--form soluble complexes with precursor proteins in vitro.

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

An improved assay for nanomole amounts of inorganic phosphate

1979 • 2,157 citations

Speculations on the functions of the major heat shock and glucose-regulated proteins

1986 • 1,574 citations

A subfamily of stress proteins facilitates translocation of secretory and mitochondrial precursor polypeptides

1988 • 1,504 citations

An enzymic method for the trace iodination of immunoglobulins and other proteins

1969 • 1,414 citations

Homologous plant and bacterial proteins chaperone oligomeric protein assembly

1988 • 1,316 citations

70K heat shock related proteins stimulate protein translocation into microsomes

1988 • 1,276 citations

Proteins as molecular chaperones

1987 • 910 citations

GroE heat-shock proteins promote assembly of foreign prokaryotic ribulose bisphosphate carboxylase oligomers in Escherichia coli

1989 • 703 citations

E. coli mutant pleiotropically defective in the export of secreted proteins

1981 • 512 citations

Correlation of competence for export with lack of tertiary structure of the mature species: A study in vivo of maltose-binding protein in E. coli

1986 • 473 citations

Purification and properties of groE, a host protein involved in bacteriophage assembly

1979 • 426 citations

Signal sequences

1989 • 417 citations

SecA protein hydrolyzes ATP and is an essential component of the protein translocation ATPase of Escherichia coli.

1989 • 406 citations

Transient association of newly synthesized unfolded proteins with the heat-shock GroEL protein

1988 • 388 citations

Suppressor mutations that restore export of a protein with a defective signal sequence

1981 • 382 citations

Protein localization in E. coli: Is there a common step in the secretion of periplasmic and outer-membrane proteins?

1981 • 359 citations

Photocrosslinking of the signal sequence of nascent preprolactin to the 54-kilodalton polypeptide of the signal recognition particle.

1986 • 337 citations

The antifolding activity of SecB promotes the export of the E. coli maltose-binding protein

1988 • 326 citations

RNA Synthesis Initiates In Vitro Conversion of M13 DNA to Its Replicative Form

1972 • 304 citations

A signal sequence receptor in the endoplasmic reticulum membrane

1987 • 291 citations

Evidence for specificity at an early step in protein export in Escherichia coli

1985 • 272 citations

Isolation and characterization of the host protein groE involved in bacteriophage lambda assembly

1979 • 272 citations

Mutations in a new gene, secB, cause defective protein localization in Escherichia coli

1983 • 270 citations

Trigger factor: a soluble protein that folds pro-OmpA into a membrane-assembly-competent form.

1987 • 257 citations

Modulation of Folding Pathways of Exported Proteins by the Leader Sequence

1988 • 243 citations

Purified secB protein of Escherichia coli retards folding and promotes membrane translocation of the maltose-binding protein in vitro.

1988 • 243 citations

SecA protein is required for secretory protein translocation into E. coli membrane vesicles

1988 • 239 citations

Translocation of domains of nascent periplasmic proteins across the cytoplasmic membrane is independent of elongation

1983 • 234 citations

Unity in Function in the Absence of Consensus in Sequence: Role of Leader Peptides in Export

1989 • 222 citations

Detection of prokaryotic signal peptidase in an Escherichia coli membrane fraction: endoproteolytic cleavage of nascent f1 pre-coat protein.

1978 • 209 citations

SecA protein, a peripheral protein of the Escherichia coli plasma membrane, is essential for the functional binding and translocation of proOmpA.

1989 • 206 citations

Synthesis, assembly into the cytoplasmic membrane, and proteolytic processing of the precursor of coliphage M13 coat protein.

1980 • 200 citations

ProOmpA is stabilized for membrane translocation by either purified E. coli trigger factor or canine signal recognition particle

1988 • 196 citations

Uncoupling Translocation from Translation: Implications for Transport of Proteins Across Membranes

1986 • 195 citations

Seventy-kilodalton heat shock proteins and an additional component from reticulocyte lysate stimulate import of M13 procoat protein into microsomes.

1988 • 187 citations

Suppression of the Escherichia coli dnaA46 mutation by amplification of the groES and groEL genes

1986 • 181 citations

ATP is essential for protein translocation into Escherichia coli membrane vesicles.

1985 • 163 citations

The “trigger factor cycle” includes ribosomes, presecretory proteins, and the plasma membrane

1988 • 162 citations

Primary structure of major outer membrane protein II (ompA protein) of Escherichia coli K-12.

1980 • 156 citations

Both ATP and the electrochemical potential are required for optimal assembly of pro-OmpA into Escherichia coli inner membrane vesicles.

1986 • 142 citations

ProOmpA spontaneously folds in a membrane assembly competent state which trigger factor stabilizes.

1988 • 135 citations

Cell biology: An unfolding story of protein translocation

1986 • 126 citations

Effects of Escherichia coli secB mutations on pre-maltose binding protein conformation and export kinetics.

1988 • 117 citations

Identification of a host protein necessary for bacteriophage morphogenesis (the groE gene product).

1978 • 103 citations

The mature portion of Escherichia coli maltose-binding protein (MBP) determines the dependence of MBP on SecB for export

1989 • 97 citations

Bacterial leader peptidase, a membrane protein without a leader peptide, uses the same export pathway as pre-secretory proteins

1984 • 84 citations

Characterization of the Escherichia coli protein-export gene secB

1989 • 69 citations

Preprotein conformation: the year's major theme in translocation studies

1988 • 63 citations