Residues essential for the function of SecE, a membrane component of the Escherichia coli secretion apparatus, are located in a conserved cytoplasmic region.

Basic local alignment search tool

1990 • 87,358 citations

The "megaprimer" method of site-directed mutagenesis.

1990 • 1,174 citations

The binding cascade of SecB to SecA to SecYE mediates preprotein targeting to the E. coli plasma membrane

1990 • 561 citations

The purified E. coli integral membrane protein is sufficient for reconstitution of SecA-dependent precursor protein translocation

1990 • 516 citations

A mammalian homolog of SEC61p and SECYp is associated with ribosomes and nascent polypeptides during translocation

1992 • 450 citations

THE ENZYMOLOGY OF PROTEIN TRANSLOCATION ACROSS THE Escherichia coli PLASMA MEMBRANE

1991 • 442 citations

GENETIC ANALYSIS OF PROTEIN EXPORT IN ESCHERICHIA COLI K12

1985 • 432 citations

SecA protein hydrolyzes ATP and is an essential component of the protein translocation ATPase of Escherichia coli.

1989 • 406 citations

Suppressor mutations that restore export of a protein with a defective signal sequence

1981 • 382 citations

Determinants of membrane protein topology.

1987 • 301 citations

Topology analysis of the SecY protein, an integral membrane protein involved in protein export in Escherichia coli.

1987 • 223 citations

A signal sequence is not required for protein export in prlA mutants of Escherichia coli.

1993 • 219 citations

Signal peptides open protein-conducting channels in E. coli

1992 • 216 citations

Reconstitution of a protein translocation system containing purified SecY, SecE, and SecA from Escherichia coli.

1991 • 212 citations

SecD is involved in the release of translocated secretory proteins from the cytoplasmic membrane of Escherichia coli.

1993 • 190 citations

Deep penetration of a portion of Escherichia coli SecA protein into model membranes is promoted by anionic phospholipids and by partial unfolding.

1992 • 183 citations

Thesec andprl genes ofEscherichia coli

1990 • 174 citations

The secE gene encodes an integral membrane protein required for protein export in Escherichia coli.

1989 • 166 citations

The nucleotide sequence of the gene for malF protein, an inner membrane component of the maltose transport system of Escherichia coli. Repeated DNA sequences are found in the malE-malF intercistronic region.

1984 • 141 citations

One of three transmembrane stretches is sufficient for the functioning of the SecE protein, a membrane component of the E. coli secretion machinery.

1991 • 136 citations

PrlA (SecY) and PrlG (SecE) interact directly and function sequentially during protein translocation in E. coli

1990 • 129 citations

Sequence and transcriptional pattern of the essential Escherichia coli secE-nusG operon

1990 • 123 citations

PrlA suppressor mutations cluster in regions corresponding to three distinct topological domains.

1993 • 115 citations

SecE‐dependent overproduction of SecY in Escherichia coli

1990 • 111 citations

New suppressors of signal-sequence mutations, prlG, are linked tightly to the secE gene of Escherichia coli.

1989 • 80 citations

The carboxyl-terminal region of SecE interacts with SecY and is functional in the reconstitution of protein translocation activity in Escherichia coli.

1992 • 68 citations

Suppressor analysis suggests a multistep, cyclic mechanism for protein secretion in Escherichia coli.

1992 • 65 citations

Isolation and characterization of the secE homologue gene of Bacillus subtilis

1993 • 63 citations

Identification of a gene fragment which codes for the 364 amino-terminal amino acid residues of a SecA homologue from Bacillus subtilis: further evidence for the conservation of the protein export apparatus in gram-positive and gram-negative bacteria

1991 • 56 citations

The organization and expression of essential transcription translation component genes in the extremely thermophilic eubacterium Thermotoga maritima

1992 • 51 citations

Identification of a chloroplast-encoded secA gene homologue in a chromophytic alga: possible role in chloroplast protein translocation

1992 • 47 citations

SecA is plastid-encoded in a red alga: implications for the evolution of plastid genomes and the thylakoid protein import apparatus

1993 • 45 citations

SecA protein is required for translocation of a model precursor protein into inverted vesicles of Escherichia coli plasma membrane.

1993 • 43 citations

Use of gene fusions to isolate promoter mutants in the transfer RNA gene tyrT of Escherichia coli

1979 • 36 citations

Cloning and molecular characterization of the secY genes from Bacillus licheniformis and Staphylococcus carnosus: comparative analysis of nine members of the SecY family

1992 • 27 citations

Identification of the gene encoding transcription factor NusG of Thermus thermophilus

1992 • 20 citations