The conformation of SecA, as revealed by its protease sensitivity, is altered upon interaction with ATP, presecretory proteins, everted membrane vesicles, and phospholipids.

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Phospholipid model membranes. I. Structural characteristics of hydrated liquid crystals

1967 • 589 citations

The ATPase activity of secA is regulated by acidic phospholipids, secY, and the leader and mature domains of precursor proteins

1990 • 551 citations

Binding of Diffusible Molecules by Macromolecules: Rapid Measurement by Rate of Dialysis

1969 • 517 citations

E. coli mutant pleiotropically defective in the export of secreted proteins

1981 • 512 citations

SecA protein hydrolyzes ATP and is an essential component of the protein translocation ATPase of Escherichia coli.

1989 • 406 citations

Phospholipid model membranes. II. Permeability properties of hydrated liquid crystals

1967 • 359 citations

Specific Chemical Cleavage in High Yield at the Amino Peptide Bonds of Cysteine and Cystine Residues

1973 • 325 citations

Cardiolipin activation of dnaA protein, the initiation protein of replication in Escherichia coli.

1988 • 266 citations

SecA protein is required for secretory protein translocation into E. coli membrane vesicles

1988 • 239 citations

SecA interacts with secretory proteins by recognizing the positive charge at the amino terminus of the signal peptide in Escherichia coli.

1990 • 229 citations

SecA protein, a peripheral protein of the Escherichia coli plasma membrane, is essential for the functional binding and translocation of proOmpA.

1989 • 206 citations

ProOmpA is stabilized for membrane translocation by either purified E. coli trigger factor or canine signal recognition particle

1988 • 196 citations

Nucleotide sequence of the secA gene and secA(Ts) mutations preventing protein export in Escherichia coli

1988 • 188 citations

[32] Purification, reconstitution, and characterization of the lac permease of Escherichia coli

1986 • 174 citations

ATP is essential for protein translocation into Escherichia coli membrane vesicles.

1985 • 163 citations

Translocation of ProOmpA possessing an intramolecular disulfide bridge into membrane vesicles of Escherichia coli. Effect of membrane energization.

1990 • 141 citations

ProOmpA spontaneously folds in a membrane assembly competent state which trigger factor stabilizes.

1988 • 135 citations

Identification of a new gene (secA) and gene product involved in the secretion of envelope proteins in Escherichia coli

1982 • 134 citations

In vitro translocation of protein across Escherichia coli membrane vesicles requires both the proton motive force and ATP.

1987 • 130 citations

Complementation of two overlapping fragments of SecA, a protein translocation ATPase of Escherichia coli, allows ATP binding to its amino-terminal region.

1990 • 115 citations

Specific recognition of the leader region of precursor proteins is required for the activation of translocation ATPase of Escherichia coli.

1989 • 107 citations

Proton Motive Force-dependent and -independent Protein Translocation Revealed by an Efficient in Vitro Assay System of Escherichia coli

1989 • 107 citations

Isolation and characterization of two outer membrane preparations from Escherichia coli

1975 • 100 citations

Novel secA alleles improve export of maltose-binding protein synthesized with a defective signal peptide

1989 • 99 citations

Interactions of divalent cations or basic proteins with phosphatidylethanolamine vesicles

1977 • 97 citations

A high Concentration of SecA Allows Proton Motive Force-independent Translocation of a Model Secretory Protein into Escherichia coli Membrane Vesicles

1989 • 92 citations

The proton motive force lowers the level of ATP required for the in vitro translocation of a secretory protein in Escherichia coli.

1990 • 85 citations

SecA protein is directly involved in protein secretion in Escherichia coli

1989 • 68 citations

SecA suppresses the temperature-sensitive SecY24 defect in protein translocation in Escherichia coli membrane vesicles.

1988 • 62 citations

The use of extragenic suppressors to define genes involved in protein export in Escherichia coli

1984 • 49 citations

Roles of cell surface components of Escherichia coli K-12 in bacteriophage T4 infection: interaction of tail core with phospholipids

1982 • 37 citations

Roles of the Pribnow box in positive regulation of the ompC and ompF genes in Escherichia coli

1987 • 27 citations

In vitro translocation of bacterial secretory proteins and energy requirements

1990 • 18 citations

Energy-dependent in vitro Translocation of a Model Protein into Escherichia Coli Inverted Membrane Vesicles Can Take Place Efficiently in the Complete Absence of the Cytosol Fraction

1989 • 17 citations