SecA interacts with secretory proteins by recognizing the positive charge at the amino terminus of the signal peptide in Escherichia coli.

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1984 • 6,459 citations

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

1982 • 6,153 citations

E. coli mutant pleiotropically defective in the export of secreted proteins

1981 • 512 citations

SecA protein hydrolyzes ATP and is an essential component of the protein translocation ATPase of Escherichia coli.

1989 • 406 citations

Transient association of newly synthesized unfolded proteins with the heat-shock GroEL protein

1988 • 388 citations

Regulation of a membrane component required for protein secretion in escherichia coli

1982 • 356 citations

Three pure chaperone proteins of Escherichia coli--SecB, trigger factor and GroEL--form soluble complexes with precursor proteins in vitro.

1989 • 317 citations

Purified secB protein of Escherichia coli retards folding and promotes membrane translocation of the maltose-binding protein in vitro.

1988 • 243 citations

SecA protein is required for secretory protein translocation into E. coli membrane vesicles

1988 • 239 citations

A defined mutation in the protein export gene within the spc ribosomal protein operon of Escherichia coli: isolation and characterization of a new temperature-sensitive secY mutant.

1984 • 216 citations

A temperature-sensitive mutant of E. coli exhibiting slow processing of exported proteins

1983 • 215 citations

Effects of the complete removal of basic amino acid residues from the signal peptide on secretion of lipoprotein in Escherichia coli.

1983 • 214 citations

SecA protein, a peripheral protein of the Escherichia coli plasma membrane, is essential for the functional binding and translocation of proOmpA.

1989 • 206 citations

Nucleotide sequence of the secA gene and secA(Ts) mutations preventing protein export in Escherichia coli

1988 • 188 citations

Export of Protein: A Biochemical View

1987 • 184 citations

ATP is essential for protein translocation into Escherichia coli membrane vesicles.

1985 • 163 citations

Both ATP and the electrochemical potential are required for optimal assembly of pro-OmpA into Escherichia coli inner membrane vesicles.

1986 • 142 citations

ProOmpA spontaneously folds in a membrane assembly competent state which trigger factor stabilizes.

1988 • 135 citations

In vitro translocation of protein across Escherichia coli membrane vesicles requires both the proton motive force and ATP.

1987 • 130 citations

The SecY membrane component of the bacterial protein export machinery: analysis by new electrophoretic methods for integral membrane proteins.

1985 • 129 citations

Proton Motive Force-dependent and -independent Protein Translocation Revealed by an Efficient in Vitro Assay System of Escherichia coli

1989 • 107 citations

Introduction of basic amino acid residues after the signal peptide inhibits protein translocation across the cytoplasmic membrane of Escherichia coli. Relation to the orientation of membrane proteins.

1988 • 100 citations

Novel secA alleles improve export of maltose-binding protein synthesized with a defective signal peptide

1989 • 99 citations

A high Concentration of SecA Allows Proton Motive Force-independent Translocation of a Model Secretory Protein into Escherichia coli Membrane Vesicles

1989 • 92 citations

Analysis of mutational alterations in the hydrophilic segment of the maltose-binding protein signal peptide

1989 • 79 citations

Role of amino-terminal positive charge on signal peptide in staphylokinase export across the cytoplasmic membrane of Escherichia coli.

1987 • 69 citations

SecA protein is directly involved in protein secretion in Escherichia coli

1989 • 68 citations

SecA suppresses the temperature-sensitive SecY24 defect in protein translocation in Escherichia coli membrane vesicles.

1988 • 62 citations

Efficient in vitro translocation into Escherichia coli membrane vesicles of a protein carrying an uncleavable signal peptide. Characterization of the translocation process.

1988 • 60 citations

Genetic Studies on Protein Export in Bacteria

1986 • 45 citations

In vitro kinetic analysis of the role of the positive charge at the amino-terminal region of signal peptides in translocation of secretory protein across the cytoplasmic membrane in Escherichia coli.

1990 • 44 citations

The role of the positively charged N-terminus of the signal sequence of E. coli outer membrane protein PhoE in export

1989 • 44 citations

Energy-dependent in vitro Translocation of a Model Protein into Escherichia Coli Inverted Membrane Vesicles Can Take Place Efficiently in the Complete Absence of the Cytosol Fraction

1989 • 17 citations