The C Terminus of SecA Is Involved in Both Lipid Binding and SecB Binding

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Experiments in molecular genetics

1972 • 28,260 citations

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

1986 • 6,284 citations

The complete general secretory pathway in gram-negative bacteria

1993 • 1,668 citations

[16] Plasmids of Escherichia coli as cloning vectors

1979 • 655 citations

The binding cascade of SecB to SecA to SecYE mediates preprotein targeting to the E. coli plasma membrane

1990 • 561 citations

The ATPase activity of secA is regulated by acidic phospholipids, secY, and the leader and mature domains of precursor proteins

1990 • 551 citations

E. coli mutant pleiotropically defective in the export of secreted proteins

1981 • 512 citations

ΔμH+ and ATP function at different steps of the catalytic cycle of preprotein translocase

1991 • 456 citations

SecA protein hydrolyzes ATP and is an essential component of the protein translocation ATPase of Escherichia coli.

1989 • 406 citations

Regulation of a membrane component required for protein secretion in escherichia coli

1982 • 356 citations

Radiolabeling of proteins by reductive alkylation with [14C]formaldehyde and sodium cyanoborohydride

1978 • 317 citations

Phosphatidylglycerol is involved in protein translocation across Escherichia coli inner membranes

1988 • 263 citations

Purified secB protein of Escherichia coli retards folding and promotes membrane translocation of the maltose-binding protein in vitro.

1988 • 243 citations

SecA interacts with secretory proteins by recognizing the positive charge at the amino terminus of the signal peptide in Escherichia coli.

1990 • 229 citations

Nucleotide sequence of the secA gene and secA(Ts) mutations preventing protein export in Escherichia coli

1988 • 188 citations

Deep penetration of a portion of Escherichia coli SecA protein into model membranes is promoted by anionic phospholipids and by partial unfolding.

1992 • 183 citations

SecA insertion into phospholipids is stimulated by negatively charged lipids and inhibited by ATP: a monolayer study

1992 • 175 citations

Negatively charged phospholipids restore prePhoE translocation across phosphatidylglycerol-depleted Escherichia coli inner membranes

1991 • 159 citations

SecA protein needs both acidic phospholipids and SecY/E protein for functional high-affinity binding to the Escherichia coli plasma membrane.

1991 • 150 citations

SecA, the peripheral subunit of the Escherichia coli precursor protein translocase, is functional as a dimer

1993 • 139 citations

In vitro translocation of protein across Escherichia coli membrane vesicles requires both the proton motive force and ATP.

1987 • 130 citations

Peptide Binding by Chaperone SecB: Implications for Recognition of Nonnative Structure

1992 • 120 citations

Complementation of two overlapping fragments of SecA, a protein translocation ATPase of Escherichia coli, allows ATP binding to its amino-terminal region.

1990 • 115 citations

SecA, an essential component of the secretory machinery of Escherichiacoli, exists as homodimer

1991 • 108 citations

The conformation of SecA, as revealed by its protease sensitivity, is altered upon interaction with ATP, presecretory proteins, everted membrane vesicles, and phospholipids.

1991 • 100 citations

Novel secA alleles improve export of maltose-binding protein synthesized with a defective signal peptide

1989 • 99 citations

Construction of a lethal mutation in the synthesis of the major acidic phospholipids of Escherichia coli.

1987 • 90 citations

SecA protein autogenously represses its own translation during normal protein secretion in Escherichia coli

1989 • 74 citations

Alteration of phospholipid composition by combined defects in phosphatidylserine and cardiolipin synthases and physiological consequences in Escherichia coli

1985 • 72 citations

SecB Protein Stabilizes a Translocation-Competent State of Purified prePhoE Protein

1989 • 67 citations

OmpF-Lpp signal sequence mutants with varying charge hydrophobicity ratios provide evidence for a phosphatidylglycerol-signal sequence interaction during protein translocation across the Escherichia coli inner membrane

1993 • 61 citations

A dual role for phosphatidylglycerol in protein translocation across the Escherichia coli inner membrane.

1994 • 59 citations

Characterization of Escherichia coli SecA protein binding to a site on its mRNA involved in autoregulation.

1991 • 43 citations

Nucleotide and negatively charged lipid‐dependent vesicle aggregation caused by SecA

1993 • 33 citations

Regulation of Escherichia coli secA mRNA translation by a secretion-responsive element

1991 • 32 citations

Carboxy-Terminal Region of Escherichia coli SecA ATPase Is Important to Promote Its Protein Translocation Activity in Vivo

1994 • 29 citations

Phosphatidylglycerol dependent protein translocation across the Escherichia coli inner membrane is inhibited by the anti‐cancer drug doxorubicin

1993 • 22 citations

Energy-dependent in vitro Translocation of a Model Protein into Escherichia Coli Inverted Membrane Vesicles Can Take Place Efficiently in the Complete Absence of the Cytosol Fraction

1989 • 17 citations

The components of the Escherichia coli protein-transport apparatus

1993 • 4 citations