Determinants of the quantity of the stable SecY complex in the Escherichia coli cell

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

1982 • 6,153 citations

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

1987 • 5,413 citations

Experiments With Gene Fusions

1984 • 2,487 citations

The ATPase activity of secA is regulated by acidic phospholipids, secY, and the leader and mature domains of precursor proteins

1990 • 551 citations

The purified E. coli integral membrane protein is sufficient for reconstitution of SecA-dependent precursor protein translocation

1990 • 516 citations

THE ENZYMOLOGY OF PROTEIN TRANSLOCATION ACROSS THE Escherichia coli PLASMA MEMBRANE

1991 • 442 citations

GENETIC ANALYSIS OF PROTEIN EXPORT IN ESCHERICHIA COLI K12

1985 • 432 citations

SecA protein hydrolyzes ATP and is an essential component of the protein translocation ATPase of Escherichia coli.

1989 • 406 citations

GENETIC ANALYSIS OF PROTEIN EXPORT IN ESCHERICHIA COLI

1990 • 291 citations

lac permease of Escherichia coli: topology and sequence elements promoting membrane insertion.

1990 • 248 citations

Topology analysis of the SecY protein, an integral membrane protein involved in protein export in Escherichia coli.

1987 • 223 citations

A defined mutation in the protein export gene within the spc ribosomal protein operon of Escherichia coli: isolation and characterization of a new temperature-sensitive secY mutant.

1984 • 216 citations

A temperature-sensitive mutant of E. coli exhibiting slow processing of exported proteins

1983 • 215 citations

Reconstitution of a protein translocation system containing purified SecY, SecE, and SecA from Escherichia coli.

1991 • 212 citations

The secE gene encodes an integral membrane protein required for protein export in Escherichia coli.

1989 • 166 citations

A mutation affecting the regulation of a secA-lacZ fusion defines a new sec gene.

1988 • 143 citations

One of three transmembrane stretches is sufficient for the functioning of the SecE protein, a membrane component of the E. coli secretion machinery.

1991 • 136 citations

The SecY membrane component of the bacterial protein export machinery: analysis by new electrophoretic methods for integral membrane proteins.

1985 • 129 citations

PrlA (SecY) and PrlG (SecE) interact directly and function sequentially during protein translocation in E. coli

1990 • 129 citations

Secy protein, a membrane-embedded secretion factor of E. coli, is cleaved by the ompT protease in vitro

1990 • 127 citations

Sequence and transcriptional pattern of the essential Escherichia coli secE-nusG operon

1990 • 123 citations

Identification of the secY (prlA) gene product involved in protein export in Escherichia coli

1984 • 119 citations

SecY, SecE, and band 1 form the membrane-embedded domain of Escherichia coli preprotein translocase.

1992 • 113 citations

SecE‐dependent overproduction of SecY in Escherichia coli

1990 • 111 citations

In vivo analysis of integration of membrane proteins in Escherichia coli

1991 • 92 citations

Structure and organization of Escherichia coli genes involved in biosynthesis of the deazaguanine derivative queuine, a nutrient factor for eukaryotes

1991 • 74 citations

The carboxyl-terminal region of SecE interacts with SecY and is functional in the reconstitution of protein translocation activity in Escherichia coli.

1992 • 68 citations

Multicopy suppression: an approach to understanding intracellular functioning of the protein export system

1992 • 56 citations

SecY and integral membrane components of the Escherichia coli protein translocation system

1992 • 53 citations

SecY variants that interfere with Escherichia coli protein export in the presence of normal secY

1992 • 37 citations

Overproduction, isolation and determination of the amino-terminal sequence of the SecY protein, a membrane protein involved in protein export in Escherichia coli

1986 • 14 citations

Chapter 8 Membrane Components of the Protein Secretion Machinery

1991 • 2 citations