Precursor protein translocation by the Escherichia coli translocase is directed by the protonmotive force.

Two dimensional thin layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots

1970 • 3,260 citations

The binding cascade of SecB to SecA to SecYE mediates preprotein targeting to the E. coli plasma membrane

1990 • 561 citations

The ATPase activity of secA is regulated by acidic phospholipids, secY, and the leader and mature domains of precursor proteins

1990 • 551 citations

The purified E. coli integral membrane protein is sufficient for reconstitution of SecA-dependent precursor protein translocation

1990 • 516 citations

ΔμH+ and ATP function at different steps of the catalytic cycle of preprotein translocase

1991 • 456 citations

THE ENZYMOLOGY OF PROTEIN TRANSLOCATION ACROSS THE Escherichia coli PLASMA MEMBRANE

1991 • 442 citations

GENETIC ANALYSIS OF PROTEIN EXPORT IN ESCHERICHIA COLI K12

1985 • 432 citations

SecA protein hydrolyzes ATP and is an essential component of the protein translocation ATPase of Escherichia coli.

1989 • 406 citations

Three pure chaperone proteins of Escherichia coli--SecB, trigger factor and GroEL--form soluble complexes with precursor proteins in vitro.

1989 • 317 citations

In vivo evidence for the role of the epsilon subunit as an inhibitor of the proton-translocating ATPase of Escherichia coli

1984 • 309 citations

Studies on cytochrome oxidase. Interactions of the cytochrome oxidase protein with phospholipids and cytochrome c.

1975 • 294 citations

Phosphatidylglycerol is involved in protein translocation across Escherichia coli inner membranes

1988 • 263 citations

Photophosphorylation in Halobacterium halobium

1974 • 246 citations

Purified secB protein of Escherichia coli retards folding and promotes membrane translocation of the maltose-binding protein in vitro.

1988 • 243 citations

SecA interacts with secretory proteins by recognizing the positive charge at the amino terminus of the signal peptide in Escherichia coli.

1990 • 229 citations

Reconstitution of a protein translocation system containing purified SecY, SecE, and SecA from Escherichia coli.

1991 • 212 citations

Detection of prokaryotic signal peptidase in an Escherichia coli membrane fraction: endoproteolytic cleavage of nascent f1 pre-coat protein.

1978 • 209 citations

SecA protein, a peripheral protein of the Escherichia coli plasma membrane, is essential for the functional binding and translocation of proOmpA.

1989 • 206 citations

ProOmpA is stabilized for membrane translocation by either purified E. coli trigger factor or canine signal recognition particle

1988 • 196 citations

The secE gene encodes an integral membrane protein required for protein export in Escherichia coli.

1989 • 166 citations

ATP is essential for protein translocation into Escherichia coli membrane vesicles.

1985 • 163 citations

Negatively charged phospholipids restore prePhoE translocation across phosphatidylglycerol-depleted Escherichia coli inner membranes

1991 • 159 citations

An outer membrane protein (OmpA) of Escherichia coli K-12 undergoes a conformational change during export.

1986 • 151 citations

Molecular chaperones and protein translocation across the Escherichia coli inner membrane

1991 • 143 citations

Both ATP and the electrochemical potential are required for optimal assembly of pro-OmpA into Escherichia coli inner membrane vesicles.

1986 • 142 citations

Translocation of ProOmpA possessing an intramolecular disulfide bridge into membrane vesicles of Escherichia coli. Effect of membrane energization.

1990 • 141 citations

The requirement for energy during export of beta-lactamase in Escherichia coli is fulfilled by the total protonmotive force.

1984 • 132 citations

PrlA (SecY) and PrlG (SecE) interact directly and function sequentially during protein translocation in E. coli

1990 • 129 citations

Identification of the secY (prlA) gene product involved in protein export in Escherichia coli

1984 • 119 citations

The nature of information, required for export and sorting, present within the outer membrane protein OmpA of Escherichia coli K-12.

1985 • 114 citations

Specific recognition of the leader region of precursor proteins is required for the activation of translocation ATPase of Escherichia coli.

1989 • 107 citations

Proton Motive Force-dependent and -independent Protein Translocation Revealed by an Efficient in Vitro Assay System of Escherichia coli

1989 • 107 citations

In vitro Analysis of the Process of Translocation of OmpA across the Escherichia coli Cytoplasmic Membrane

1989 • 103 citations

Energetically distinct early and late stages of HlyB/HlyD-dependent secretion across both Escherichia coli membranes.

1991 • 102 citations

The conformation of SecA, as revealed by its protease sensitivity, is altered upon interaction with ATP, presecretory proteins, everted membrane vesicles, and phospholipids.

1991 • 100 citations

A high Concentration of SecA Allows Proton Motive Force-independent Translocation of a Model Secretory Protein into Escherichia coli Membrane Vesicles

1989 • 92 citations

SecA protein: Autoregulated initiator of secretory precursor protein translocation across theE. coli plasma membrane

1990 • 87 citations

The proton motive force lowers the level of ATP required for the in vitro translocation of a secretory protein in Escherichia coli.

1990 • 85 citations

Incorporation of beef heart cytochrome c oxidase as a proton-motive force-generating mechanism in bacterial membrane vesicles.

1985 • 78 citations

Biochemical Analysis of Spontaneous fepA Mutants of Escherichia coli

1988 • 73 citations

Proton transfer is rate-limiting for translocation of precursor proteins by the Escherichia coli translocase.

1991 • 70 citations

SecA suppresses the temperature-sensitive SecY24 defect in protein translocation in Escherichia coli membrane vesicles.

1988 • 62 citations

Translocation of Pro-OmpA across Inner Membrane Vesicles of Escherichia coli Occurs in Two Consecutive Energetically Distinct Steps

1989 • 60 citations

Escherichia coli exports previously folded and biotinated protein domains

1991 • 59 citations

Measurements of the proton motive force generated by cytochrome c oxidase from Bacillus subtilis in proteoliposomes and membrane vesicles

1986 • 47 citations

The signal sequence suffices to direct export of outer membrane protein OmpA of Escherichia coli K-12

1987 • 42 citations

Purification of SecE and reconstitution of SecE‐dependent protein translocation activity

1991 • 41 citations

Roles of H+-ATPase and proton motive force in ATP-dependent protein translocation in vitro

1986 • 39 citations

Electrochemical potential releases a membrane-bound secretion intermediate of maltose-binding protein in Escherichia coli

1990 • 35 citations

Effects of nucleotides on ATP-dependent protein translocation into Escherichia coli membrane vesicles

1986 • 33 citations

A proline residue near the amino terminus of the mature domain of secretory proteins lowers the level of the proton motive force required for translocation

1991 • 29 citations

A sequence correlation between oppositely charged residues in secreted proteins

1980 • 7 citations