Charged residues render pro-OmpA potential dependent for initiation of membrane translocation

Experiments in molecular genetics

1972 • 28,260 citations

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA

1972 • 2,842 citations

Patterns of Amino Acids near Signal‐Sequence Cleavage Sites

1983 • 2,290 citations

Two differentially regulated mRNAs with different 5′ ends encode secreted and intracellular forms of yeast invertase

1982 • 1,539 citations

The spontaneous insertion of proteins into and across membranes: The helical hairpin hypothesis

1981 • 868 citations

Multiple Mechanisms of Protein Insertion into and Across Membranes

1985 • 720 citations

ΔμH+ and ATP function at different steps of the catalytic cycle of preprotein translocase

1991 • 456 citations

GENETIC ANALYSIS OF PROTEIN EXPORT IN ESCHERICHIA COLI K12

1985 • 432 citations

Mechanism of Incorporation of Cell Envelope Proteins in Escherichia Coli

1982 • 344 citations

Use of gene fusion to study secretion of maltose-binding protein into Escherichia coli periplasm

1979 • 267 citations

SecA protein is required for secretory protein translocation into E. coli membrane vesicles

1988 • 239 citations

Fine-tuning the topology of a polytopic membrane protein: Role of positively and negatively charged amino acids

1990 • 221 citations

Effects of two sec genes on protein assembly into the plasma membrane of Escherichia coli.

1985 • 217 citations

SecA protein, a peripheral protein of the Escherichia coli plasma membrane, is essential for the functional binding and translocation of proOmpA.

1989 • 206 citations

Synthesis, assembly into the cytoplasmic membrane, and proteolytic processing of the precursor of coliphage M13 coat protein.

1980 • 200 citations

The isolation of homogeneous leader peptidase from a strain of Escherichia coli which overproduces the enzyme.

1982 • 179 citations

Net N-C charge imbalance may be important for signal sequence function in bacteria

1986 • 176 citations

ATP is essential for protein translocation into Escherichia coli membrane vesicles.

1985 • 163 citations

Alteration of the amino terminus of the mature sequence of a periplasmic protein can severely affect protein export in Escherichia coli.

1988 • 163 citations

Importance of secondary structure in the signal sequence for protein secretion.

1983 • 152 citations

Use of gene fusions to study outer membrane protein localization in Escherichia coli.

1977 • 148 citations

Both ATP and the electrochemical potential are required for optimal assembly of pro-OmpA into Escherichia coli inner membrane vesicles.

1986 • 142 citations

The requirement for energy during export of beta-lactamase in Escherichia coli is fulfilled by the total protonmotive force.

1984 • 132 citations

Energetics and intermediates of the assembly of Protein OmpA into the outer membrane of Escherichia coli.

1983 • 114 citations

In vitro Analysis of the Process of Translocation of OmpA across the Escherichia coli Cytoplasmic Membrane

1989 • 103 citations

Introduction of basic amino acid residues after the signal peptide inhibits protein translocation across the cytoplasmic membrane of Escherichia coli. Relation to the orientation of membrane proteins.

1988 • 100 citations

A 30-residue-long "export initiation domain" adjacent to the signal sequence is critical for protein translocation across the inner membrane of Escherichia coli.

1991 • 95 citations

Leader Peptidase of Escherichia coli : Critical Role of a Small Domain in Membrane Assembly

1987 • 72 citations

A conservative amino acid substitution, arginine for lysine, abolishes export of a hybrid protein in Escherichia coli

1989 • 69 citations

The role of the polar, carboxyl-terminal domain of Escherichia coli leader peptidase in its translocation across the plasma membrane.

1986 • 68 citations

Illicit secretion of a cytoplasmic protein into the periplasm of Escherichia coli requires a signal peptide plus a portion of the cognate secreted protein

1989 • 66 citations

Efficient translocation of positively charged residues of M13 procoat protein across the membrane excludes electrophoresis as the primary force for membrane insertion.

1990 • 63 citations

The cytoplasmic domain of Escherichia coli leader peptidase is a "translocation poison" sequence.

1988 • 61 citations

Translocation of Pro-OmpA across Inner Membrane Vesicles of Escherichia coli Occurs in Two Consecutive Energetically Distinct Steps

1989 • 60 citations

Assembly of a functional F0 of the proton-translocating ATPase of Escherichia coli.

1983 • 60 citations

Bacterial protein translocation: kinetic and thermodynamic role of ATP and the protonmotive force

1992 • 56 citations

Export incompatibility of N-terminal basic residues in a mature polypeptide of Eschericbia coli can be alleviated by optimising the signal peptide

1990 • 55 citations

Recombinant forms of M13 procoat with an OmpA leader sequence or a large carboxy-terminal extension retain their independence of secY function.

1987 • 53 citations

Chapter 3 The Use of Genetic Techniques to Analyze Protein Export in Escherichia coli

1985 • 50 citations

[58] Synthesis of bacteriophage-specific enzymes directed by DNA in Vitro

1971 • 50 citations

Positive charges in the cytoplasmic domain of Escherichia coli leader peptidase prevent an apolar domain from functioning as a signal.

1989 • 48 citations

A proline residue near the amino terminus of the mature domain of secretory proteins lowers the level of the proton motive force required for translocation

1991 • 29 citations

In vitro translocation of secretory proteins possessing no charges at the mature domain takes place efficiently in a protonmotive force-dependent manner.

1992 • 29 citations