Suppressor analysis suggests a multistep, cyclic mechanism for protein secretion in Escherichia coli.

Experiments in molecular genetics

1972 • 28,260 citations

Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.

1975 • 3,026 citations

Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu

1976 • 1,895 citations

Experiments with gene fusions

1985 • 1,210 citations

A protein-conducting channel in the endoplasmic reticulum

1991 • 613 citations

The binding cascade of SecB to SecA to SecYE mediates preprotein targeting to the E. coli plasma membrane

1990 • 561 citations

The purified E. coli integral membrane protein is sufficient for reconstitution of SecA-dependent precursor protein translocation

1990 • 516 citations

THE ENZYMOLOGY OF PROTEIN TRANSLOCATION ACROSS THE Escherichia coli PLASMA MEMBRANE

1991 • 442 citations

GENETIC ANALYSIS OF PROTEIN EXPORT IN ESCHERICHIA COLI K12

1985 • 432 citations

Versatile low-copy-number plasmid vectors for cloning in Escherichia coli

1982 • 361 citations

Regulation of a membrane component required for protein secretion in escherichia coli

1982 • 356 citations

A defined mutation in the protein export gene within the spc ribosomal protein operon of Escherichia coli: isolation and characterization of a new temperature-sensitive secY mutant.

1984 • 216 citations

Reconstitution of a protein translocation system containing purified SecY, SecE, and SecA from Escherichia coli.

1991 • 212 citations

Export of Protein: A Biochemical View

1987 • 184 citations

Heat-shock proteins DnaK and GroEL facilitate export of LacZ hybrid proteins in E. coli

1990 • 175 citations

Thesec andprl genes ofEscherichia coli

1990 • 174 citations

Mutations affecting localization of an Escherichia coli outer membrane protein, the bacteriophage λ receptor

1980 • 153 citations

A mutation affecting the regulation of a secA-lacZ fusion defines a new sec gene.

1988 • 143 citations

One of three transmembrane stretches is sufficient for the functioning of the SecE protein, a membrane component of the E. coli secretion machinery.

1991 • 136 citations

PrlA (SecY) and PrlG (SecE) interact directly and function sequentially during protein translocation in E. coli

1990 • 129 citations

SecY, SecE, and band 1 form the membrane-embedded domain of Escherichia coli preprotein translocase.

1992 • 113 citations

SecE‐dependent overproduction of SecY in Escherichia coli

1990 • 111 citations

Characterization of cold-sensitive secY mutants of Escherichia coli

1990 • 107 citations

Genetic studies on the inability of beta-galactosidase to be translocated across the Escherichia coli cytoplasmic membrane

1989 • 90 citations

SecA protein: Autoregulated initiator of secretory precursor protein translocation across theE. coli plasma membrane

1990 • 87 citations

New suppressors of signal-sequence mutations, prlG, are linked tightly to the secE gene of Escherichia coli.

1989 • 80 citations

SecA suppresses the temperature-sensitive SecY24 defect in protein translocation in Escherichia coli membrane vesicles.

1988 • 62 citations

PrlA is important for the translocation of exported proteins across the cytoplasmic membrane of Escherichia coli.

1989 • 50 citations

The use of extragenic suppressors to define genes involved in protein export in Escherichia coli

1984 • 49 citations

Reconstitution of protein translocation from detergent-solubilized Escherichia coli inverted vesicles: PrlA protein-deficient vesicles efficiently translocate precursor proteins.

1990 • 45 citations

Purification of SecE and reconstitution of SecE‐dependent protein translocation activity

1991 • 41 citations

SecB protein: A cytosolic export factor that associates with nascent exported proteins

1990 • 41 citations

Export of the periplasmic maltose-binding protein ofEscherichia coli

1990 • 31 citations

A progenitor of the outer membrane LamB trimer

1988 • 26 citations

In vitro translocation of bacterial secretory proteins and energy requirements

1990 • 18 citations