The SecA and SecY subunits of translocase are the nearest neighbors of a translocating preprotein, shielding it from phospholipids.

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

1959 • 47,127 citations

Short-Term Effects of Nose-Only Cigarette Smoke Exposure on Glutathione Redox Homeostasis, Cytochrome P450 1A1/2 and Respiratory Enzyme Activities in Mice Tissues

2013 • 47,043 citations

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

1985 • 15,222 citations

Silver staining of proteins in polyacrylamide gels

1981 • 3,565 citations

Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.

1975 • 3,026 citations

A general method ofin vitropreparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions

1988 • 2,410 citations

Surface-specific iodination of membrane proteins of viruses and eucaryotic cells using 1,3,4,6-tetrachloro-3α,6α-diphenylglycoluril

1978 • 912 citations

The spontaneous insertion of proteins into and across membranes: The helical hairpin hypothesis

1981 • 868 citations

A one-step purification of membrane proteins using a high efficiency immunomatrix.

1982 • 782 citations

Signal recognition particle contains a 7S RNA essential for protein translocation across the endoplasmic reticulum

1982 • 766 citations

Binding of a specific ligand inhibits import of a purified precursor protein into mitochondria

1986 • 723 citations

The binding cascade of SecB to SecA to SecYE mediates preprotein targeting to the E. coli plasma membrane

1990 • 561 citations

The ATPase activity of secA is regulated by acidic phospholipids, secY, and the leader and mature domains of precursor proteins

1990 • 551 citations

The purified E. coli integral membrane protein is sufficient for reconstitution of SecA-dependent precursor protein translocation

1990 • 516 citations

E. coli mutant pleiotropically defective in the export of secreted proteins

1981 • 512 citations

Transmembrane Protein Structure: Spin Labeling of Bacteriorhodopsin Mutants

1990 • 465 citations

ΔμH+ and ATP function at different steps of the catalytic cycle of preprotein translocase

1991 • 456 citations

THE ENZYMOLOGY OF PROTEIN TRANSLOCATION ACROSS THE Escherichia coli PLASMA MEMBRANE

1991 • 442 citations

The Assembly of Proteins into Biological Membranes: The Membrane Trigger Hypothesis

1979 • 408 citations

Suppressor mutations that restore export of a protein with a defective signal sequence

1981 • 382 citations

Sec61p and BiP directly facilitate polypeptide translocation into the ER

1992 • 370 citations

Trans‐membrane Translocation of Proteins

1979 • 364 citations

Refolding of an integral membrane protein. Denaturation, renaturation, and reconstitution of intact bacteriorhodopsin and two proteolytic fragments.

1981 • 332 citations

A protein of the endoplasmic reticulum involved early in polypeptide translocation

1992 • 322 citations

Three pure chaperone proteins of Escherichia coli--SecB, trigger factor and GroEL--form soluble complexes with precursor proteins in vitro.

1989 • 317 citations

In vivo evidence for the role of the epsilon subunit as an inhibitor of the proton-translocating ATPase of Escherichia coli

1984 • 309 citations

A 42K outer-membrane protein is a component of the yeast mitochondrial protein import site

1989 • 274 citations

Evidence for specificity at an early step in protein export in Escherichia coli

1985 • 272 citations

Phosphatidylglycerol is involved in protein translocation across Escherichia coli inner membranes

1988 • 263 citations

Purified secB protein of Escherichia coli retards folding and promotes membrane translocation of the maltose-binding protein in vitro.

1988 • 243 citations

Signal peptides open protein-conducting channels in E. coli

1992 • 216 citations

Detection of prokaryotic signal peptidase in an Escherichia coli membrane fraction: endoproteolytic cleavage of nascent f1 pre-coat protein.

1978 • 209 citations

SecA protein, a peripheral protein of the Escherichia coli plasma membrane, is essential for the functional binding and translocation of proOmpA.

1989 • 206 citations

MOLECULAR GENETICS OF MEMBRANE PHOSPHOLIPID SYNTHESIS

1986 • 206 citations

Synthesis, assembly into the cytoplasmic membrane, and proteolytic processing of the precursor of coliphage M13 coat protein.

1980 • 200 citations

ProOmpA is stabilized for membrane translocation by either purified E. coli trigger factor or canine signal recognition particle

1988 • 196 citations

Mapping of the protein import machinery in the mitochondrial outer membrane by crosslinking of translocation intermediates

1992 • 185 citations

Formation of transmembrane tubules by spontaneous polymerization of the hydrophilic complement protein C9

1982 • 183 citations

Thesec andprl genes ofEscherichia coli

1990 • 174 citations

Polypeptides traverse the mitochondrial envelope in an extended state

1990 • 172 citations

Symmetrical distribution of phospholipids in the membrane of Bacillus megaterium

1977 • 167 citations

The secE gene encodes an integral membrane protein required for protein export in Escherichia coli.

1989 • 166 citations

SecA protein needs both acidic phospholipids and SecY/E protein for functional high-affinity binding to the Escherichia coli plasma membrane.

1991 • 150 citations

A mutation affecting the regulation of a secA-lacZ fusion defines a new sec gene.

1988 • 143 citations

A chimeric mitochondrial precursor protein with internal disulfide bridges blocks import of authentic precursors into mitochondria and allows quantitation of import sites.

1988 • 143 citations

Translocation of ProOmpA possessing an intramolecular disulfide bridge into membrane vesicles of Escherichia coli. Effect of membrane energization.

1990 • 141 citations

Yeast Sec proteins interact with polypeptides traversing the endoplasmic reticulum membrane

1992 • 138 citations

One of three transmembrane stretches is sufficient for the functioning of the SecE protein, a membrane component of the E. coli secretion machinery.

1991 • 136 citations

Translocation can drive the unfolding of a preprotein domain.

1993 • 127 citations

Photogenerated reagents for membrane labeling. 1. Phenylnitrene formed within the lipid bilayer

1978 • 123 citations

Structure-function studies on bacteriorhodopsin. IV. Purification and renaturation of bacterio-opsin polypeptide expressed in Escherichia coli.

1987 • 123 citations

Escherichia coli SecB protein associates with exported protein precursors in vivo.

1989 • 123 citations

M13 procoat inserts into liposomes in the absence of other membrane proteins.

1985 • 121 citations

Adriamycin, a drug interacting with acidic phospholipids, blocks import of precursor proteins by isolated yeast mitochondria

1989 • 120 citations

Gene structure of the OmpA protein, a major surface protein of Escherichia coli required for cell-cell interaction

1980 • 115 citations

ProOmpA contains secondary and tertiary structure prior to translocation and is shielded from aggregation by association with SecB protein.

1990 • 114 citations

Import of honeybee prepromelittin into the endoplasmic reticulum: structural basis for independence of SRP and docking protein.

1987 • 113 citations

SecY, SecE, and band 1 form the membrane-embedded domain of Escherichia coli preprotein translocase.

1992 • 113 citations

Specific recognition of the leader region of precursor proteins is required for the activation of translocation ATPase of Escherichia coli.

1989 • 107 citations

Induction of non-bilayer lipid structures by functional signal peptides.

1990 • 107 citations

Novel secA alleles improve export of maltose-binding protein synthesized with a defective signal peptide

1989 • 99 citations

New suppressors of signal-sequence mutations, prlG, are linked tightly to the secE gene of Escherichia coli.

1989 • 80 citations

Large aqueous channels in membrane vesicles derived from the rough endoplasmic reticulum of canine pancreas or the plasma membrane of Escherichia coli.

1989 • 74 citations

The carboxyl-terminal region of SecE interacts with SecY and is functional in the reconstitution of protein translocation activity in Escherichia coli.

1992 • 68 citations

Investigations on the insertion of the mitochondrial precursor protein apocytochrome c into model membranes

1985 • 66 citations

Reconstitution of rapid and asymmetric assembly of M13 procoat protein into liposomes which have bacterial leader peptidase.

1983 • 65 citations

Membrane assembly from purified components. II. Assembly of M13 procoat into liposomes reconstituted with purified leader peptidase

1981 • 60 citations

Structural studies on transmembrane proteins. 1. Model study using bacteriorhodopsin mutants containing single cysteine residues

1989 • 39 citations

Conformational and membrane-binding properties of a signal sequence are largely unaltered by its adjacent mature region.

1991 • 35 citations