SecA protein, a peripheral protein of the Escherichia coli plasma membrane, is essential for the functional binding and translocation of proOmpA.

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

E. coli mutant pleiotropically defective in the export of secreted proteins

1981 • 512 citations

Suppressor mutations that restore export of a protein with a defective signal sequence

1981 • 382 citations

Regulation of a membrane component required for protein secretion in escherichia coli

1982 • 356 citations

In vivo evidence for the role of the epsilon subunit as an inhibitor of the proton-translocating ATPase of Escherichia coli

1984 • 309 citations

Sequence of the leader peptidase gene of Escherichia coli and the orientation of leader peptidase in the bacterial envelope.

1983 • 307 citations

Leader peptidase catalyzes the release of exported proteins from the outer surface of the Escherichia coli plasma membrane.

1985 • 292 citations

Mutations in a new gene, secB, cause defective protein localization in Escherichia coli

1983 • 270 citations

Thespcrtbosomal protein operon ofEschenchia coli:sequence and cotranscriptlon of the rlbosomal protein genes and a protein export gene

1983 • 258 citations

Trigger factor: a soluble protein that folds pro-OmpA into a membrane-assembly-competent form.

1987 • 257 citations

SecA protein is required for secretory protein translocation into E. coli membrane vesicles

1988 • 239 citations

Translocation of domains of nascent periplasmic proteins across the cytoplasmic membrane is independent of elongation

1983 • 234 citations

Topology analysis of the SecY protein, an integral membrane protein involved in protein export in Escherichia coli.

1987 • 223 citations

A temperature-sensitive mutant of E. coli exhibiting slow processing of exported proteins

1983 • 215 citations

Detection of prokaryotic signal peptidase in an Escherichia coli membrane fraction: endoproteolytic cleavage of nascent f1 pre-coat protein.

1978 • 209 citations

Synthesis, assembly into the cytoplasmic membrane, and proteolytic processing of the precursor of coliphage M13 coat protein.

1980 • 200 citations

ProOmpA is stabilized for membrane translocation by either purified E. coli trigger factor or canine signal recognition particle

1988 • 196 citations

The isolation of homogeneous leader peptidase from a strain of Escherichia coli which overproduces the enzyme.

1982 • 179 citations

In vitro translocation of bacterial proteins across the plasma membrane of Escherichia coli.

1984 • 174 citations

ATP is essential for protein translocation into Escherichia coli membrane vesicles.

1985 • 163 citations

The “trigger factor cycle” includes ribosomes, presecretory proteins, and the plasma membrane

1988 • 162 citations

Both ATP and the electrochemical potential are required for optimal assembly of pro-OmpA into Escherichia coli inner membrane vesicles.

1986 • 142 citations

ProOmpA spontaneously folds in a membrane assembly competent state which trigger factor stabilizes.

1988 • 135 citations

Mechanism of signal peptide cleavage in the biosynthesis of the major lipoprotein of the Escherichia coli outer membrane.

1982 • 133 citations

A previously unidentified gene in the spc operon of Escherichia coli K12 specifies a component of the protein export machinery

1982 • 126 citations

Identification of the secY (prlA) gene product involved in protein export in Escherichia coli

1984 • 119 citations

Mechanisms of membrane assembly: effects of energy poisons on the conversion of soluble M13 coliphage procoat to membrane-bound coat protein.

1980 • 115 citations

Transfer of proteins into mitochondria. Precursor to the ADP/ATP carrier binds to receptor sites on isolated mitochondria.

1983 • 98 citations

High-affinity binding sites involved in the import of porin into mitochondria.

1987 • 92 citations

Prolipoprotein signal peptidase in Escherichia coli is distinct from the M13 procoat protein signal peptidase.

1982 • 89 citations

Energy-requiring translocation of the OmpA protein and alkaline phosphatase of Escherichia coli into inner membrane vesicles

1984 • 74 citations

Nucleotide sequence of the lspA gene, the structural gene for lipoprotein signal peptidase of Escherichia coli

1984 • 66 citations

SecA suppresses the temperature-sensitive SecY24 defect in protein translocation in Escherichia coli membrane vesicles.

1988 • 62 citations

Translational and post-translational cleavage of M13 procoat protein: extracts of both the cytoplasmic and outer membranes of Escherichia coli contain leader peptidase activity.

1979 • 61 citations

The use of extragenic suppressors to define genes involved in protein export in Escherichia coli

1984 • 49 citations