Thesec andprl genes ofEscherichia coli

A simple method for displaying the hydropathic character of a protein

1982 • 22,471 citations

Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.

1975 • 3,026 citations

Patterns of Amino Acids near Signal‐Sequence Cleavage Sites

1983 • 2,290 citations

A Genetic Approach to Analyzing Membrane Protein Topology

1986 • 610 citations

E. coli mutant pleiotropically defective in the export of secreted proteins

1981 • 512 citations

GENETIC ANALYSIS OF PROTEIN EXPORT IN ESCHERICHIA COLI K12

1985 • 432 citations

SecA protein hydrolyzes ATP and is an essential component of the protein translocation ATPase of Escherichia coli.

1989 • 406 citations

Suppressor mutations that restore export of a protein with a defective signal sequence

1981 • 382 citations

Regulation of a membrane component required for protein secretion in escherichia coli

1982 • 356 citations

The antifolding activity of SecB promotes the export of the E. coli maltose-binding protein

1988 • 326 citations

Mutations that alter the signal sequence of alkaline phosphatase in Escherichia coli

1983 • 281 citations

Evidence for specificity at an early step in protein export in Escherichia coli

1985 • 272 citations

Mutations in a new gene, secB, cause defective protein localization in Escherichia coli

1983 • 270 citations

Use of gene fusion to study secretion of maltose-binding protein into Escherichia coli periplasm

1979 • 267 citations

Thespcrtbosomal protein operon ofEschenchia coli:sequence and cotranscriptlon of the rlbosomal protein genes and a protein export gene

1983 • 258 citations

Alkaline phosphatase fusions: sensors of subcellular location

1990 • 254 citations

SecA protein is required for secretory protein translocation into E. coli membrane vesicles

1988 • 239 citations

Effects of two sec genes on protein assembly into the plasma membrane of Escherichia coli.

1985 • 217 citations

A defined mutation in the protein export gene within the spc ribosomal protein operon of Escherichia coli: isolation and characterization of a new temperature-sensitive secY mutant.

1984 • 216 citations

A temperature-sensitive mutant of E. coli exhibiting slow processing of exported proteins

1983 • 215 citations

Conditional-lethal mutations that suppress genetic defects in morphogenesis by altering structural proteins.

1975 • 212 citations

SecA protein, a peripheral protein of the Escherichia coli plasma membrane, is essential for the functional binding and translocation of proOmpA.

1989 • 206 citations

Sequence analysis of mutations that prevent export of λ receptor, an Escherichia coli outer membrane protein

1980 • 195 citations

Nucleotide sequence of the secA gene and secA(Ts) mutations preventing protein export in Escherichia coli

1988 • 188 citations

Genetic analysis of the membrane insertion and topology of MalF, a cytoplasmic membrane protein of Escherichia coli

1988 • 175 citations

secD, a new gene involved in protein export in Escherichia coli

1987 • 173 citations

Synthesis and processing of an Escherichia coli alkaline phosphatase precursor in vitro.

1977 • 171 citations

The secE gene encodes an integral membrane protein required for protein export in Escherichia coli.

1989 • 166 citations

Mutations affecting localization of an Escherichia coli outer membrane protein, the bacteriophage λ receptor

1980 • 153 citations

A mutation affecting the regulation of a secA-lacZ fusion defines a new sec gene.

1988 • 143 citations

Identification of a new gene (secA) and gene product involved in the secretion of envelope proteins in Escherichia coli

1982 • 134 citations

Mutational Alteration of the Maximal Level of Lac Operon Expression

1966 • 130 citations

Escherichia coli mutants accumulating the precursor of a secreted protein in the cytoplasm

1979 • 129 citations

Production in Escherichia coli and one‐step purification of bifunctional hybrid proteins which bind maltose

1988 • 127 citations

A previously unidentified gene in the spc operon of Escherichia coli K12 specifies a component of the protein export machinery

1982 • 126 citations

Kinetic analysis of lamB mutants suggests the signal sequence plays multiple roles in protein export.

1986 • 107 citations

SecB functions as a cytosolic signal recognition factor for protein export in E. coli

1989 • 107 citations

Mutations altering the cellular localization of the phage lambda receptor, an Escherichia coli outer membrane protein.

1978 • 106 citations

Export of unprocessed precursor maltose-binding protein to the periplasm of Escherichia coli cells

1987 • 106 citations

Conversion of beta-galactosidase to a membrane-bound state by gene fusion.

1976 • 100 citations

Novel secA alleles improve export of maltose-binding protein synthesized with a defective signal peptide

1989 • 99 citations

The mature portion of Escherichia coli maltose-binding protein (MBP) determines the dependence of MBP on SecB for export

1989 • 97 citations

Genetic studies on the inability of beta-galactosidase to be translocated across the Escherichia coli cytoplasmic membrane

1989 • 90 citations

New suppressors of signal-sequence mutations, prlG, are linked tightly to the secE gene of Escherichia coli.

1989 • 80 citations

Sequence information within the lamB gene is required for proper routing of the bacteriophage λ receptor protein to the outer membrane of Escherichia coli K-12

1982 • 79 citations

Analysis of mutational alterations in the hydrophilic segment of the maltose-binding protein signal peptide

1989 • 79 citations

Evidence for a coupling of synthesis and export of an outer membrane protein in Escherichia coli.

1983 • 78 citations

SecA protein autogenously represses its own translation during normal protein secretion in Escherichia coli

1989 • 74 citations

Proper interaction between at least two components is required for efficient export of proteins to the Escherichia coli cell envelope

1985 • 74 citations

Suppression of growth and protein secretion defects in Escherichia coli secA mutants by decreasing protein synthesis

1986 • 69 citations

Characterization of the Escherichia coli protein-export gene secB

1989 • 69 citations

Post-translational export of maltose-binding protein in Escherichia coli strains harboring malE signal sequence mutations and either prl+ or prl suppressor alleles.

1985 • 63 citations

Localization and purification of two enzymes from Escherichia coli capable of hydrolyzing a signal peptide.

1986 • 61 citations

Membrane assembly from purified components. II. Assembly of M13 procoat into liposomes reconstituted with purified leader peptidase

1981 • 60 citations

Sequence information required for protein translocation from the cytoplasm

1987 • 58 citations

Biochemical evidence for the secY24 defect in Escherichia coli protein translocation and its suppression by soluble cytoplasmic factors.

1987 • 56 citations

Effect of cortisol, prolactin and ADH on the amniotic membrane

1975 • 56 citations

Regulation of the Escherichia coli secA gene by protein secretion defects: analysis of secA, secB, secD, and secY mutants

1988 • 56 citations

Mutation that suppresses the protein export defect of the secY mutation and causes cold-sensitive growth of Escherichia coli

1984 • 54 citations

The product of gene secC is involved in the synthesis of exported proteins in E. coli

1984 • 52 citations

PrlA is important for the translocation of exported proteins across the cytoplasmic membrane of Escherichia coli.

1989 • 50 citations

Isolation and characterization of outer membrane permeability mutants in Escherichia coli K-12

1985 • 50 citations

Identification of five new essential genes involved in the synthesis of a secreted protein in Escherichia coli

1985 • 49 citations

The use of extragenic suppressors to define genes involved in protein export in Escherichia coli

1984 • 49 citations

Distinct mutation sites in prlA suppressor mutant strains of Escherichia coli respond either to suppression of signal peptide mutations or to blockage of staphylokinase processing

1988 • 48 citations

Escherichia coli K-12 mutants that allow transport of maltose via the beta-galactoside transport system

1979 • 44 citations

Mutation prlF1 relieves the lethality associated with export of beta-galactosidase hybrid proteins in Escherichia coli

1984 • 33 citations

Beta-galactosidase gene fusions as probes for the cytoplasmic regions of subunits I and II of the membrane-bound cytochrome d terminal oxidase from Escherichia coli.

1988 • 33 citations

PrlC, a suppressor of signal sequence mutations in Escherichia coli, can direct the insertion of the signal sequence into the membrane

1989 • 24 citations

Increased expression of the bifunctional protein PrlF suppresses overproduction lethality associated with exported beta-galactosidase hybrid proteins in Escherichia coli

1990 • 22 citations

Inhibition and resumption of processing of the staphylokinase in some Escherichia coli prlA suppressor mutants.

1988 • 18 citations