Molecular characterization of an operon (hyp) necessary for the activity of the three hydrogenase isoenzymes in Escherichia coli

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Experiments in molecular genetics

1972 • 28,260 citations

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

1985 • 15,222 citations

Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid

1978 • 4,472 citations

Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing

1984 • 4,231 citations

A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.

1985 • 3,185 citations

DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.

1987 • 2,266 citations

TECHNOLOGICAL EXAMINATION OF LOW‐FIRED TERRACOTTA STATUES FROM AYIA IRINI, KEA

1982 • 1,253 citations

Evidence for two functional gal promoters in intact Escherichia coli cells.

1981 • 1,022 citations

Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences.

1979 • 940 citations

Recognition of protein coding regions in DNA sequences

1982 • 927 citations

Use of the ‘Perceptron’ algorithm to distinguish translational initiation sites inE. coli

1982 • 660 citations

An absolute method for protein determination based on difference in absorbance at 235 and 280 nm

1980 • 592 citations

Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage : In vivo probe for transcriptional control sequences

1979 • 451 citations

Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli

1985 • 342 citations

Nucleotide sequence and expression of an operon in Escherichia coli coding for formate hydrogenylase components

1990 • 293 citations

Differential expression of hydrogenase isoenzymes in Escherichia coli K-12: evidence for a third isoenzyme

1985 • 260 citations

Structure-independent nucleotide sequence analysis.

1979 • 199 citations

Divergent promoters, a common form of gene organization

1988 • 195 citations

The genetic map of the filamentous bacteriophage f1

1972 • 190 citations

FORMIC DEHYDROGENASE AND THE HYDROGENLYASE ENZYME COMPLEX IN COLI-AEROGENES BACTERIA

1957 • 187 citations

A sensitive immunoblotting method for measuring protein synthesis initiation factor levels in lysates of Escherichia coli.

1981 • 184 citations

The identification of mutants ofEscherichia colideficient in formate dehydrogenase and nitrate reductase activities using dye indicator plates

1977 • 146 citations

Cloning and sequencing of a putative Escherichia coli [NiFe] hydrogenase-1 operon containing six open reading frames

1990 • 140 citations

Regulation and Over-expression of the fnr Gene of Escherichia coli

1987 • 138 citations

Novel transcriptional control of the pyruvate formate-lyase gene: upstream regulatory sequences and multiple promoters regulate anaerobic expression

1989 • 137 citations

Isolation and characterisation of a soluble active fragment of hydrogenase isoenzyme 2 from the membranes of anaerobically grown Escherichia coli

1986 • 136 citations

Purification and properties of membrane-bound hydrogenase isoenzyme 1 from anaerobically grown Escherichia coli K12

1986 • 130 citations

Genetic and physiological characterization of new Escherichia coli mutants impaired in hydrogenase activity

1986 • 124 citations

Pleiotropic hydrogenase mutants of Escherichia coli K12: growth in the presence of nickel can restore hydrogenase activity

1986 • 122 citations

On the redox control of synthesis of anaerobically induced enzymes in enterobacteriaceae

1983 • 116 citations

Involvement of the ntrA gene product in the anaerobic metabolism of Escherichia coli

1987 • 116 citations

Identification and sequence analysis of the gene encoding the transcriptional activator of the formate hydrogenlyase system of Escherichia coli

1990 • 106 citations

Factors affecting transcriptional regulation of the formate-hydrogen-lyase pathway of Escherichia coli

1987 • 104 citations

Anaerobic and leucine-dependent expression of a peptide transport gene in Salmonella typhimurium

1984 • 98 citations

The genetic and biochemical basis of the transformability of Escherichia coli K12

1972 • 89 citations

Characterization and physiological roles of membrane-bound hydrogenase isoenzymes from Salmonella typhimurium

1986 • 80 citations

Divergent promoters, a common form of gene organization.

1988 • 79 citations

Isolation and characterization of mutant strains of Escherichia coli altered in H2 metabolism

1985 • 66 citations

Characterization of divergent NtrA‐dependent promoters in the anaerobically expressed gene cluster coding for hydrogenase 3 components of Escherichia coli

1990 • 58 citations

Two-dimensional gel electrophoretic analysis of Escherichia coli proteins: influence of various anaerobic growth conditions and the fnr gene product on cellular protein composition

1988 • 49 citations

Cloning of hydrogenase genes and fine structure analysis of an operon essential for H2 metabolism in Escherichia coli

1985 • 47 citations

Biochemical and genetic analysis of hydrogen metabolism in Escherichia coli: the hydB gene

1988 • 39 citations

Gene-product relationships of fhlA and fdv genes of Escherichia coli

1988 • 31 citations

Mutations in trans which affect the anaerobic expression of a formate dehydrogenase (fdhF) structural gene

1989 • 28 citations

Immunoblotting Analysis of Ribosomal Proteins from Archaebacteria

1984 • 26 citations

Hydrogen metabolism in Escherichia coli: biochemical and genetic evidence for a hydF gene

1988 • 21 citations

Randomly induced Escherichia coli K-12 Tn5 insertion mutants defective in hydrogenase activity

1989 • 19 citations

Isolation of Genes Required for Hydrogenase Synthesis in Escherichia coli

1987 • 18 citations