Proteolysis and class I major histocompatibility complex antigen presentation

Inhibitors of the proteasome block the degradation of most cell proteins and the generation of peptides presented on MHC class I molecules

1994 • 2,480 citations

STRUCTURE AND FUNCTIONS OF THE 20S AND 26S PROTEASOMES

1996 • 2,408 citations

Structure of 20S proteasome from yeast at 2.4Å resolution

1997 • 2,211 citations

The ubiquitin-proteasome proteolytic pathway

1994 • 1,778 citations

MHC ligands and peptide motifs: first listing

1995 • 1,674 citations

Inhibition of Proteasome Activities and Subunit-Specific Amino-Terminal Threonine Modification by Lactacystin

1995 • 1,601 citations

The Proteasome: Paradigm of a Self-Compartmentalizing Protease

1998 • 1,483 citations

Proteasome inhibitors: a novel class of potent and effective antitumor agents.

1999 • 1,447 citations

THE UBIQUITIN SYSTEM FOR PROTEIN DEGRADATION

1992 • 1,402 citations

MECHANISMS OF MHC CLASS I–RESTRICTED ANTIGEN PROCESSING

1998 • 1,017 citations

A Subcomplex of the Proteasome Regulatory Particle Required for Ubiquitin-Conjugate Degradation and Related to the COP9-Signalosome and eIF3

1998 • 901 citations

Fcγ Receptor–mediated Induction of Dendritic Cell Maturation and Major Histocompatibility Complex Class I–restricted Antigen Presentation after Immune Complex Internalization

1999 • 898 citations

A Phagosome-to-Cytosol Pathway for Exogenous Antigens Presented on MHC Class I Molecules

1995 • 882 citations

Inhibition of antigen processing by the internal repeat region of the Epstein–Barr virus nuclear antigen-1

1995 • 820 citations

Peptides Naturally Presented by MHC Class I Molecules

1993 • 803 citations

Ornithine decarboxylase is degraded by the 26S proteasome without ubiquitination

1992 • 780 citations

Phagocytic processing of bacterial antigens for class I MHC presentation to T cells

1993 • 625 citations

UBIQUITIN AND THE CONTROL OF PROTEIN FATE IN THE SECRETORY AND ENDOCYTIC PATHWAYS

1998 • 617 citations

ANTIGEN PROCESSING AND PRESENTATION BY THE CLASS I MAJOR HISTOCOMPATIBILITY COMPLEX

1996 • 613 citations

Cytotoxic T-cell immunity to virus-infected non-haematopoietic cells requires presentation of exogenous antigen

1999 • 586 citations

γ-Interferon and expression of MHC genes regulate peptide hydrolysis by proteasomes

1993 • 572 citations

The Sizes of Peptides Generated from Protein by Mammalian 26 and 20 S Proteasomes

1999 • 565 citations

A soluble ATP-dependent proteolytic system responsible for the degradation of abnormal proteins in reticulocytes.

1977 • 553 citations

Inhibition of ubiquitin/proteasome-dependent protein degradation by the Gly-Ala repeat domain of the Epstein–Barr virus nuclear antigen 1

1997 • 549 citations

Thermolability of ubiquitin-activating enzyme from the mammalian cell cycle mutant ts85

1984 • 522 citations

HLA-A2.1-Associated Peptides from a Mutant Cell Line: A Second Pathway of Antigen Presentation

1992 • 522 citations

MHC Class I Expression in Mice Lacking the Proteasome Subunit LMP-7

1994 • 520 citations

The Active Sites of the Eukaryotic 20 S Proteasome and Their Involvement in Subunit Precursor Processing

1997 • 506 citations

HLA-A2 molecules in an antigen-processing mutant cell contain signal sequence-derived peptides

1992 • 502 citations

Universality and Structure of the N-end Rule

1989 • 472 citations

Proteasome Inhibition Leads to a Heat-shock Response, Induction of Endoplasmic Reticulum Chaperones, and Thermotolerance

1997 • 464 citations

The multicatalytic proteinase complex, a major extralysosomal proteolytic system

1990 • 464 citations

The N-end rule

1992 • 464 citations

MHC-linked LMP gene products specifically alter peptidase activities of the proteasome

1993 • 453 citations

Proteasome-dependent endoplasmic reticulum-associated protein degradation: An unconventional route to a familiar fate

1996 • 451 citations

Altered peptidase and viral-specific T cell response in LMP2 mutant mice

1994 • 450 citations

The mechanism and functions of ATP‐dependent proteases in bacterial and animal cells

1992 • 450 citations

Covalent modification of the active site threonine of proteasomal β subunits and theEscherichia colihomolog HslV by a new class of inhibitors

1997 • 441 citations

Defective ribosomal products (DRiPs): a major source of antigenic peptides for MHC class I molecules?

1996 • 440 citations

Identification, purification, and characterization of a protein activator (PA28) of the 20 S proteasome (macropain).

1992 • 439 citations

A proteasome-related gene between the two ABC transporter loci in the class II region of the human MHC

1991 • 427 citations

Lactacystin, Proteasome Function, and Cell Fate

1998 • 420 citations

Class I MHC presentation of exogenous soluble antigen via macropinocytosis in bone marrow macrophages

1995 • 416 citations

An HLA-A2-restricted tyrosinase antigen on melanoma cells results from posttranslational modification and suggests a novel pathway for processing of membrane proteins.

1996 • 416 citations

Ubiquitin-Mediated Pathways for Intracellular Proteolysis

1987 • 409 citations

Selective Inhibitors of the Proteasome-dependent and Vacuolar Pathways of Protein Degradation in Saccharomyces cerevisiae

1996 • 395 citations

Lactacystin and clasto-Lactacystin β-Lactone Modify Multiple Proteasome β-Subunits and Inhibit Intracellular Protein Degradation and Major Histocompatibility Complex Class I Antigen Presentation

1997 • 388 citations

Purification of an 11 S regulator of the multicatalytic protease.

1992 • 384 citations

Phagocytic processing of exogenous particulate antigens by macrophages for presentation by class I MHC molecules.

1994 • 383 citations

Structure of Peptides Associated with Class I and Class II MHC Molecules

1994 • 381 citations

Interferon-γ Induces Different Subunit Organizations and Functional Diversity of Proteasomes1

1994 • 371 citations

Targeting of substrates to the 26S proteasome

1997 • 362 citations

Constitutive macropinocytosis allows TAP‐dependent major histocompatibility compex class I presentation of exogenous soluble antigen by bone marrow‐derived dendritic cells

1997 • 351 citations

Second proteasome-related gene in the human MHC class II region

1991 • 348 citations

A role for the proteasome regulator PA28α in antigen presentation

1996 • 341 citations

Presentation of Exogenous Antigen with Class I Major Histocompatibility Complex Molecules

1990 • 338 citations

Defective presentation to class I-restricted cytotoxic T lymphocytes in vaccinia-infected cells is overcome by enhanced degradation of antigen.

1988 • 331 citations

Evidence for the presence of five distinct proteolytic components in the pituitary multicatalytic proteinase complex. Properties of two components cleaving bonds on the carboxyl side of branched chain and small neutral amino acids

1993 • 323 citations

A role for the ubiquitin-dependent proteolytic pathway in MHC class l-restricted antigen presentation

1993 • 317 citations

Interferon gamma stimulation modulates the proteolytic activity and cleavage site preference of 20S mouse proteasomes.

1994 • 314 citations

Structural and serological similarity of MHC-linked LMP and proteasome (multicatalytic proteinase) complexes

1991 • 310 citations

Efficient processing of an antigenic sequence for presentation by MHC class I molecules depends on its neighboring residues in the protein

1991 • 303 citations

Interferon-γ Can Stimulate Post-proteasomal Trimming of the N Terminus of an Antigenic Peptide by Inducing Leucine Aminopeptidase

1998 • 297 citations

Identification of the yeast 20S proteasome catalytic centers and subunit interactions required for active-site formation

1997 • 295 citations

Coordinated Dual Cleavages Induced by the Proteasome Regulator PA28 Lead to Dominant MHC Ligands

1996 • 287 citations

A novel site for ubiquitination: the N-terminal residue, and not internal lysines of MyoD, is essential for conjugation and degradation of the protein

1998 • 281 citations

A proteolytic system that compensates for loss of proteasome function

1998 • 271 citations

Two distinct proteolytic processes in the generation of a major histocompatibility complex class I-presented peptide

1997 • 267 citations

Contribution of Proteasomal β-Subunits to the Cleavage of Peptide Substrates Analyzed with Yeast Mutants

1998 • 266 citations

Human CD8 + T Cell Responses to EBV EBNA1: HLA Class I Presentation of the (Gly-Ala)–Containing Protein Requires Exogenous Processing

1997 • 261 citations

PRE2, highly homologous to the human major histocompatibility complex-linked RING10 gene, codes for a yeast proteasome subunit necessary for chrymotryptic activity and degradation of ubiquitinated proteins.

1993 • 260 citations

Homology of proteasome subunits to a major histocompatibility complex-linked LMP gene

1991 • 256 citations

Proteasome subunits encoded by the major histocompatibility complex are not essential for antigen presentation

1992 • 250 citations

Proteasome Inhibitors Cause Induction of Heat Shock Proteins and Trehalose, Which Together Confer Thermotolerance in Saccharomyces cerevisiae

1998 • 226 citations

Contribution of proteasome-mediated proteolysis to the hierarchy of epitopes presented by major histocompatibility complex class I molecules

1995 • 225 citations

Displacement of housekeeping proteasome subunits by MHC-encoded LMPs: a newly discovered mechanism for modulating the multicatalytic proteinase complex.

1994 • 220 citations

Simultaneous binding of PA28 and PA700 activators to 20 S proteasomes

1998 • 219 citations

Proteasome subunits encoded in the MHC are not generally required for the processing of peptides bound by MHC class I molecules

1992 • 215 citations

MHC affinity, peptide liberation, T cell repertoire, and immunodominance all contribute to the paucity of MHC class I-restricted peptides recognized by antiviral CTL.

1997 • 207 citations

Post-proline cleaving enzyme. Purification of this endopeptidase by affinity chromatography.

1976 • 202 citations

Identification of MECL-1 (LMP-10) as the third IFN-gamma-inducible proteasome subunit.

1996 • 196 citations

Newly identified pair of proteasomal subunits regulated reciprocally by interferon gamma.

1996 • 195 citations

Presentation of Exogenous Protein Antigens on Major Histocompatability Complex Class I Molecules by Dendritic Cells: Pathway of Presentation and Regulation by Cytokines

1997 • 195 citations

Flanking sequences influence the presentation of an endogenously synthesized peptide to cytotoxic T lymphocytes.

1992 • 184 citations

A Single Residue Exchange Within a Viral CTL Epitope Alters Proteasome-Mediated Degradation Resulting in Lack of Antigen Presentation

1996 • 181 citations

Substrate binding and sequence preference of the proteasome revealed by active-site-directed affinity probes

1998 • 179 citations

Targeting of HIV-1 Antigens for Rapid Intracellular Degradation Enhances Cytotoxic T Lymphocyte (CTL) Recognition and the Induction of De Novo CTL Responses In Vivo After Immunization

1997 • 176 citations

A Chinese hamster cell cycle mutant arrested at G2 phase has a temperature-sensitive ubiquitin-activating enzyme, E1.

1988 • 173 citations

Proteolytic processing of ovalbumin and beta-galactosidase by the proteasome to a yield antigenic peptides.

1994 • 172 citations

A third interferon‐γ‐induced subunit exchange in the 20S proteasome

1996 • 168 citations

Incorporation of major histocompatibility complex – encoded subunits LMP2 and LMP7 changes the quality of the 20S proteasome polypeptide processing products independent of interferon‐γ

1995 • 167 citations

Proteasome Subunits X and Y Alter Peptidase Activities in Opposite Ways to the Interferon-γ-induced Subunits LMP2 and LMP7

1996 • 164 citations

Roles of proteasomes, transporter for antigen presentation (TAP), and beta 2-microglobulin in the processing of bacterial or particulate antigens via an alternate class I MHC processing pathway.

1996 • 163 citations

Proteolytic processing of ovalbumin and beta-galactosidase by the proteasome to a yield antigenic peptides.

1994 • 160 citations

Trimming of TAP-translocated peptides in the endoplasmic reticulum and in the cytosol during recycling.

1994 • 151 citations

Cis-trans recognition and subunit-specific degradation of short-lived proteins

1990 • 150 citations

The PRE4 gene codes for a subunit of the yeast proteasome necessary for peptidylglutamyl-peptide-hydrolyzing activity. Mutations link the proteasome to stress- and ubiquitin-dependent proteolysis.

1993 • 149 citations

Purification and characterization of a protein inhibitor of the 20S proteasome (macropain)

1992 • 131 citations

Genes encoded in the major histocompatibility complex affecting the generation of peptides for TAP transport

1995 • 129 citations

Molecular properties of the proteasome activator PA28 family proteins and γ‐interferon regulation

1997 • 126 citations

Proteasomes Can Either Generate or Destroy MHC Class I Epitopes: Evidence for Nonproteasomal Epitope Generation in the Cytosol

1998 • 124 citations

Replacement of proteasome subunits X and Y by LMP7 and LMP2 induced by interferon‐γ for acquirement of the functional diversity responsible for antigen processing

1994 • 121 citations

Cell-specific Sorting of Biogenic Amine Transporters Expressed in Epithelial Cells

1996 • 121 citations

The Class I Antigen-processing Pathway for the Membrane Protein Tyrosinase Involves Translation in the Endoplasmic Reticulum and Processing in the Cytosol

1998 • 120 citations

The cleavage preference of the proteasome governs the yield of antigenic peptides.

1995 • 117 citations

Multiple proteins physically interact with PU.1. Transcriptional synergy with NF-IL6 beta (C/EBP delta, CRP3).

1995 • 111 citations

The generation of MHC class I-associated peptides is only partially inhibited by proteasome inhibitors: involvement of nonproteasomal cytosolic proteases in antigen processing?

1997 • 108 citations

Presentation of Out-of-Frame Peptide/MHC Class I Complexes by a Novel Translation Initiation Mechanism

1999 • 103 citations

Flanking residues alter antigenicity and immunogenicity of multi-unit CTL epitopes.

1996 • 102 citations

Peptide selection by MHC-encoded TAP transporters

1994 • 101 citations

Novel dipeptide aldehydes are proteasome inhibitors and block the MHC-I antigen-processing pathway.

1995 • 99 citations

Degradation of oxidized insulin B chain by the multiproteinase complex macropain (proteasome)

1991 • 99 citations

Deoxyribonucleic acid vaccines encoding antigens with rapid proteasome-dependent degradation are highly efficient inducers of cytolytic T lymphocytes.

1997 • 98 citations

LMP2+ proteasomes are required for the presentation of specific antigens to cytotoxic T lymphocytes

1995 • 97 citations

Substrate specificity of allelic variants of the TAP peptide transporter

1994 • 95 citations

MHC-encoded proteasome subunits LMP2 and LMP7 are not required for efficient antigen presentation.

1994 • 94 citations

Point mutation flanking a CTL epitope ablates in vitro and in vivo recognition of a full-length viral protein.

1997 • 93 citations

Characterization of the Proteasome Regulator PA28

1996 • 93 citations

Supply and transport of peptides presented by class I MHC molecules

1995 • 91 citations

Transporter-independent processing of HIV-1 envelope protein for recognition by CD8+ T cells

1993 • 90 citations

Potential Immunocompetence of Proteolytic Fragments Produced by Proteasomes before Evolution of the Vertebrate Immune System

1997 • 87 citations

PRE3, highly homologous to the human major histocompatibility complex‐linked LMP2 (RING12) gene, codes for a yeast proteasome subunit necessary for the peptidylglutamyl‐peptide hydrolyzing activity

1994 • 80 citations

Generation of the vesicular stomatitis virus nucleoprotein cytotoxic T lymphocyte epitope requires proteasome-dependent and -independent proteolytic activities

1998 • 78 citations

Partial purification and characterization of post-proline cleaving enzyme:

1976 • 78 citations

Allelic Differences in the Relationship Between Proteasome Activity and MHC Class I Peptide Loading

1998 • 75 citations

Introduction of a Glycosylation Site into a Secreted Protein Provides Evidence for an Alternative Antigen Processing Pathway: Transport of Precursors of Major Histocompatability Complex Class I–Restricted Peptides from the Endoplasmic Reticulum to the Cytosol

1997 • 73 citations

Altered Properties of the Branched Chain Amino Acid-preferring Activity Contribute to Increased Cleavages after Branched Chain Residues by the “Immunoproteasome”

1998 • 67 citations

20S proteasome from LMP7 knock out mice reveals altered proteolytic activities and cleavage site preferences

1996 • 66 citations

Cutting Edge: Induction of Enhanced CTL-Dependent Protective Immunity In Vivo by N-End Rule Targeting of a Model Tumor Antigen

1999 • 66 citations

Presentation of endogenous and exogenous antigens is not affected by inactivation of E1 ubiquitin-activating enzyme in temperature-sensitive cell lines.

1995 • 64 citations

Transporter (TAP)‐independent processing of a multiple membrane‐spanning protein, the Epstein‐Barr virus latent membrane protein 2

1996 • 64 citations

Dissociation of Proteasomal Degradation of Biosynthesized Viral Proteins from Generation of MHC Class I-Associated Antigenic Peptides

1998 • 64 citations

Role of Proteasomes in Antigen Presentation

1993 • 61 citations

Double‐cleavage production of the CTL epitope by proteasomes and PA28: role of the flanking region

1997 • 59 citations

Hepatitis C Virus Envelope Glycoprotein E1 Originates in the Endoplasmic Reticulum and Requires Cytoplasmic Processing for Presentation by Class I MHC Molecules

1999 • 58 citations

CTL epitope generation is tightly linked to cellular proteolysis of a Listeria monocytogenes antigen.

1996 • 58 citations

Presentation of endogenous peptide/MHC class I complexes is profoundly influenced by specific C-terminal flanking residues.

1995 • 57 citations

The effect of the proteasome inhibitor lactacystin on the presentation of transporter associated with antigen processing (TAP)-dependent and TAP-independent peptide epitopes by class I molecules.

1997 • 55 citations

Inactivation of a Defined Active Site in the Mouse 20S Proteasome Complex Enhances Major Histocompatibility Complex Class I Antigen Presentation of a Murine Cytomegalovirus Protein

1998 • 55 citations

MHC-linked low-molecular mass polypeptide subunits define distinct subsets of proteasomes. Implications for divergent function among distinct proteasome subsets.

1993 • 53 citations

Chemical denaturation and modification of ovalbumin alters its dependence on ubiquitin conjugation for class I antigen presentation.

1996 • 50 citations

Major Histocompatibility Class I Molecules Can Present Cryptic Translation Products to T-cells

1995 • 48 citations

Regulation of class I-restricted epitope processing by local or distal flanking sequence.

1997 • 47 citations

Kinetic Studies of the Branched Chain Amino Acid Preferring Peptidase Activity of the 20S Proteasome: Development of a Continuous Assay and Inhibition by Tripeptide Aldehydes and clasto-Lactacystin β-Lactone

1998 • 47 citations

Proteasome activity limits the assembly of MHC class I molecules after IFN-gamma stimulation.

1997 • 46 citations

Evidence That the Nature of Amino Acid Residues in the P3 Position Directs Substrates to Distinct Catalytic Sites of the Pituitary Multicatalytic Proteinase Complex (Proteasome)

1994 • 46 citations

Contribution of Proline Residue for Efficient Production of MHC Class I Ligands by Proteasomes

1998 • 45 citations

Human lymphoblast and erythrocyte multicatalytic proteases: differential peptidase activities and responses to the 11S regulator

1995 • 43 citations

Differential effects of flanking residues on presentation of epitopes from chimeric peptides

1994 • 40 citations

TAP peptide transporter-independent presentation of heat-killed Sendai virus antigen on MHC class I molecules by splenic antigen-presenting cells.

1997 • 38 citations

Processing of HIV-1 Envelope Glycoprotein for Class I-Restricted Recognition: Dependence on TAP1/2 and Mechanisms for Cytosolic Localization

1999 • 35 citations

Processing of HIV-1 envelope glycoprotein for class I-restricted recognition: dependence on TAP1/2 and mechanisms for cytosolic localization.

1999 • 34 citations

Class I-restricted presentation of an HIV-1 gp41 epitope containing an N-linked glycosylation site. Implications for the mechanism of processing of viral envelope proteins.

1996 • 33 citations

Generation of naturally processed peptide/MHC class I complexes is independent of the stability of endogenously synthesized precursors.

1996 • 33 citations

Constitutive and interferon-γ-induced expression of the human proteasome subunit multicatalytic endopeptidase complex-like 11EMBL accession number for MECL1 cDNA: Y13640.1

1998 • 26 citations

The proteasome inhibitor lactacystin prevents the generation of an endoplasmic reticulum leader—derived T cell epitope

1998 • 20 citations

Ubiquitin-dependent proteolysis and cell cycle control in yeast

1996 • 11 citations

Deleted Work

1955 • 0 citations