Identification of the yeast 20S proteasome catalytic centers and subunit interactions required for active-site formation

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites

1988 • 2,903 citations

Structure of 20S proteasome from yeast at 2.4Å resolution

1997 • 2,211 citations

In Vivo Half-Life of a Protein Is a Function of Its Amino-Terminal Residue

1986 • 1,942 citations

UBIQUITIN-DEPENDENT PROTEIN DEGRADATION

1996 • 1,680 citations

Inhibition of Proteasome Activities and Subunit-Specific Amino-Terminal Threonine Modification by Lactacystin

1995 • 1,601 citations

Crystal Structure of the 20 S Proteasome from the Archaeon T. acidophilum at 3.4 Å Resolution

1995 • 1,513 citations

A simple and efficient method for direct gene deletion inSaccharomyces cerevisiae

1993 • 1,256 citations

Abnormal Proteins Serve as Eukaryotic Stress Signals and Trigger the Activation of Heat Shock Genes

1986 • 904 citations

Proteasome Inhibition Leads to a Heat-shock Response, Induction of Endoplasmic Reticulum Chaperones, and Thermotolerance

1997 • 464 citations

Stable binding of Drosophila heat shock factor to head-to-head and tail-to-tail repeats of a conserved 5 bp recognition unit

1989 • 439 citations

The Cln3-Cdc28 kinase complex of S. cerevisiae is regulated by proteolysis and phosphorylation.

1992 • 414 citations

Multiple ubiquitin-conjugating enzymes participate in the in vivo degradation of the yeast MATα2 repressor

1993 • 397 citations

Autocatalytic Subunit Processing Couples Active Site Formation in the 20S Proteasome to Completion of Assembly

1996 • 390 citations

Evidence for the presence of five distinct proteolytic components in the pituitary multicatalytic proteinase complex. Properties of two components cleaving bonds on the carboxyl side of branched chain and small neutral amino acids

1993 • 323 citations

Resistance to cadmium mediated by ubiquitin-dependent proteolysis

1993 • 261 citations

Processive Degradation of Proteins and Other Catalytic Properties of the Proteasome from Thermoplasma acidophilum

1997 • 200 citations

Proteolysis: The proteasome: a protein-degrading organelle?

1995 • 155 citations

Biogenesis, structure and function of the yeast 20S proteasome.

1995 • 115 citations

The Yeast SEN3 Gene Encodes a Regulatory Subunit of the 26S Proteasome Complex Required for Ubiquitin-Dependent Protein Degradation In Vivo

1995 • 74 citations

3,4-dichloroisocoumarin-induced activation of the degradation of beta-casein by the bovine pituitary multicatalytic proteinase complex.

1992 • 36 citations

Leupeptin-binding site(s) in the mammalian multicatalytic proteinase complex

1993 • 36 citations

Nucleotide sequence ofPUP1encoding a putative proteasome subunit inSaccharomyces cerevisiae

1991 • 33 citations

Identification and localization of a cysteinyl residue critical for the trypsin-like catalytic activity of the proteasome

1992 • 32 citations

The DOA Pathway: Studies on the Functions and Mechanisms of Ubiquitin-dependent Protein Degradation in the Yeast Saccharomyces cerevisiae

1995 • 22 citations