The PRE4 gene codes for a subunit of the yeast proteasome necessary for peptidylglutamyl-peptide-hydrolyzing activity. Mutations link the proteasome to stress- and ubiquitin-dependent proteolysis.

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Molecular cloning: A laboratory manual

1990 • 85,659 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Current protocols in molecular biology

1988 • 7,584 citations

Methods in Yeast Genetics

1988 • 4,905 citations

In Vivo Half-Life of a Protein Is a Function of Its Amino-Terminal Residue

1986 • 1,942 citations

Nuclear targeting sequences — a consensus?

1991 • 1,908 citations

High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.

1979 • 1,504 citations

[19] Targeting, disruption, replacement, and allele rescue: Integrative DNA transformation in yeast

1991 • 1,369 citations

Replacement of chromosome segments with altered DNA sequences constructed in vitro.

1979 • 779 citations

Protein degradation in the endoplasmic reticulum

1990 • 659 citations

[20] In vitro mutagenesis and plasmid shuffling: From cloned gene to mutant yeast

1991 • 584 citations

Nuclear protein localization

1991 • 570 citations

Purification of two high molecular weight proteases from rabbit reticulocyte lysate.

1987 • 542 citations

The N-end rule

1992 • 464 citations

The multicatalytic proteinase complex, a major extralysosomal proteolytic system

1990 • 464 citations

A general method for polyethylene-glycol-induced genetic transformation of bacteria and yeast

1983 • 462 citations

Ubiquitin-mediated protein degradation.

1988 • 453 citations

Enzymes Required for Yeast Prohormone Processing

1988 • 446 citations

Proteinase yscE, the yeast proteasome/multicatalytic-multifunctional proteinase: mutants unravel its function in stress induced proteolysis and uncover its necessity for cell survival.

1991 • 421 citations

Ubiquitin-Mediated Pathways for Intracellular Proteolysis

1987 • 409 citations

Identity of the 19S 'prosome' particle with the large multifunctional protease complex of mammalian cells (the proteasome)

1988 • 404 citations

KAR1, a gene required for function of both intranuclear and extranuclear microtubules in yeast

1987 • 372 citations

ATP-dependent incorporation of 20S protease into the 26S complex that degrades proteins conjugated to ubiquitin.

1989 • 370 citations

The proteasome (multicatalytic protease) is a component of the 1500-kDa proteolytic complex which degrades ubiquitin-conjugated proteins.

1990 • 327 citations

Cation‐Sensitive Neutral Endopeptidase: Isolation and Specificity of the Bovine Pituitary Enzyme

1980 • 313 citations

The ubiquitin system: functions and mechanisms

1985 • 298 citations

Ubiquitin: Roles in protein modification and breakdown

1983 • 297 citations

The multicatalytic proteinase (prosome) is ubiquitous from eukaryotes to archaebacteria

1989 • 277 citations

Ubiquitin as a degradation signal.

1992 • 261 citations

Demonstration of two distinct high molecular weight proteases in rabbit reticulocytes, one of which degrades ubiquitin conjugates.

1987 • 255 citations

Purification of a liver alkaline protease which degrades oxidatively modified glutamine synthetase. Characterization as a high molecular weight cysteine proteinase.

1985 • 244 citations

The multicatalytic proteinase of mammalian cells

1989 • 242 citations

Primary structure of the Thermoplasma proteasome and its implications for the structure, function, and evolution of the multicatalytic proteinase

1992 • 235 citations

Lysosomal (Vacuolar) Proteinases of Yeast Are Essential Catalysts for Protein Degradation, Differentiation, and Cell Survival

1989 • 230 citations

Three proteolytic systems in the yeast saccharomyces cerevisiae

1991 • 228 citations

Proteasomes (multi-protease complexes) as 20 S ring-shaped particles in a variety of eukaryotic cells.

1988 • 225 citations

Ubiquitin-conjugating enzymes: novel regulators of eukaryotic cells

1990 • 184 citations

The prosome: an ubiquitous morphologically distinct RNP particle associated with repressed mRNPs and containing specific ScRNA and a characteristic set of proteins.

1984 • 180 citations

Drosophila small cytoplasmic 19S ribonucleoprotein is homologous to the rat multicatalytic proteinase

1988 • 179 citations

THE SYNTHESIS AND FUNCTION OF PROTEASES IN SACCHAROMYCES: GENETIC APPROACHES

1984 • 179 citations

The ubiquitin pathway for protein degradation

1991 • 170 citations

Involvement of the proteasome in various degradative processes in mammalian cells.

1989 • 162 citations

Plantacin B, a bacteriocin produced byLactobacillus plantarumNCDO 1193

1988 • 155 citations

Pituitary multicatalytic proteinase complex. Specificity of components and aspects of proteolytic activity

1989 • 139 citations

Proteinases, proteolysis and biological control in the yeast Saccharomyces cerevisiae

1985 • 135 citations

The sequence of theSaccharomyces cerevisiaegenePH02codes for a regulatory protein with unusual aminoacid composition

1987 • 134 citations

Possible mechanism of nuclear translocation of proteasomes

1990 • 132 citations

Proteasomes are essential for yeast proliferation. cDNA cloning and gene disruption of two major subunits.

1990 • 127 citations

How are substrates recognized by the ubiquitin-mediated proteolytic system

1989 • 125 citations

Purification and characterization of a high molecular weight proteinase (macropain) from human erythrocytes

1986 • 107 citations

Stress‐induced proteolysis in yeast

1992 • 103 citations

Molecular cloning and functional analysis of three subunits of yeast proteasome.

1991 • 97 citations

Proteolysis in eukaryotic cells. Identification of multiple proteolytic enzymes in yeast.

1984 • 95 citations

Proteinase C (carboxypeptidase Y) mutant of yeast

1975 • 91 citations

The 22 S cylinder particles of Xenopus laevis. I. Biochemical and electron microscopic characterization.

1983 • 77 citations

Proteolysis in eukaryotic cells. Proteinase yscE, a new yeast peptidase.

1984 • 76 citations

The proteasome/multicatalytic—multifunctional proteinase In vivo function in the ubiquitin‐dependent N‐end rule pathway of protein degradation in eukaryotes

1992 • 76 citations

Molecular and cell biology of yeasts

1989 • 74 citations

Proteinase yscE of yeast shows homology with the 20 S cylinder particles of Xenopus laevis

1988 • 66 citations

Prosomes, subcomplexes of untranslated mRNP

1990 • 65 citations

Biogenesis of the yeast vacuole (lysosome)

1992 • 63 citations

Identification of three high molecular mass cysteine proteinases from rat skeletal muscle

1983 • 57 citations

Proteinase action in vitro versus proteinase function in vivo: mutants shed light on intracellular proteolysis in yeast

1982 • 55 citations

A controlled breakdown: Antigen processing and the turnover of viral proteins

1992 • 54 citations

Involvement of proteasomes (multicatalytic proteinase) in ATP‐dependent proteolysis in rat reticulocyte extracts

1988 • 54 citations

New genes in the MHC that encode proteins for antigen processing

1992 • 49 citations

An enzyme related to the high molecular weight multicatalytic proteinase, macropain, participates in a ubiquitin-mediated, ATP-stimulated proteolytic pathway in soluble extracts of BHK 21/C13 fibroblasts

1988 • 36 citations

Ubiquitin, a central component of selective cytoplasmic proteolysis, is linked to proteins residing at the locus of non‐selective proteolysis, the vacuole

1992 • 36 citations

Vacuoles are not the sole compartments of proteolytic enzymes in yeast

1984 • 33 citations

Nucleotide sequence ofPUP1encoding a putative proteasome subunit inSaccharomyces cerevisiae

1991 • 33 citations

Eukaryotic protein degradation

1990 • 30 citations

PRS3 encoding an essential subunit of yeast proteasomes homologous to mammalian proteasome subunit C5

1992 • 29 citations

Molecular cloning of an essential yeast gene encoding a proteasomal subunit

1992 • 27 citations

Molecular interaction of the proteasome (multicatalytic proteinase). Evidence that the proteasome is not a constituent of the ‘26 S’ multienzyme complex

1991 • 24 citations

Genetic analysis of ubiquitin-dependent protein degradation

1992 • 19 citations

Cloning and sequence analysis of pituitary cDNA encoding the β‐subunit of Xenopus proteasome

1991 • 17 citations

19s cylinder particles ubiqutious from plants to man: Their morphology, molecular composition and potential functions

1987 • 17 citations

The suppressor gene scl1+ of Saccharomyces cerevisiae is essential for growth

1989 • 16 citations

High-molecular-mass proteinases in rabbit reticulocytes: the multicatalytic proteinase is an ATP-independent enzyme and ATP-activated proteolysis is in part associated with a cysteine proteinase complexed toα1-macroglobulin

1989 • 16 citations

Lysosomal and non-lysosomal proteolysis in the eukaryotic cell: studies on yeast

1991 • 15 citations

Evidence indicating that the multicatalytic proteinase of rabbit reticulocytes is not incorporated as a core enzyme into a 26 S proteinase complex

1992 • 10 citations

Purification and characterization of proteinase yscJ, a new yeast peptidase

1992 • 4 citations