Full-length cDNAs: more than just reaching the ends

Initial sequencing and analysis of the human genome

2001 • 23,520 citations

The Sequence of the Human Genome

2001 • 13,500 citations

An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs

1987 • 5,139 citations

A simple and very efficient method for generating cDNA libraries

1983 • 4,606 citations

Prediction of complete gene structures in human genomic DNA

1997 • 4,041 citations

DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.

1987 • 2,266 citations

eIF4 Initiation Factors: Effectors of mRNA Recruitment to Ribosomes and Regulators of Translation

1999 • 2,096 citations

Internal initiation of translation of eukaryotic mRNA directed by a sequence derived from poliovirus RNA

1988 • 1,884 citations

Molecular control of vertebrate iron metabolism: mRNA-based regulatory circuits operated by iron, nitric oxide, and oxidative stress.

1996 • 1,311 citations

High-Efficiency Cloning of Full-Length cDNA

1982 • 1,309 citations

An Integrated Nanoliter DNA Analysis Device

1998 • 1,288 citations

The HIV-1 rev trans-activator acts through a structured target sequence to activate nuclear export of unspliced viral mRNA

1989 • 1,280 citations

The human XIST gene: Analysis of a 17 kb inactive X-specific RNA that contains conserved repeats and is highly localized within the nucleus

1992 • 1,269 citations

Plasmid screening at high colony density

1980 • 1,100 citations

Exon Recognition in Vertebrate Splicing

1995 • 1,067 citations

The product of the mouse Xist gene is a 15 kb inactive X-specific transcript containing no conserved ORF and located in the nucleus

1992 • 983 citations

Interpreting cDNA sequences: Some insights from studies on translation

1996 • 785 citations

High-efficiency cloning of full-length cDNA.

1982 • 763 citations

A Family of Insulin-Like Growth Factor II mRNA-Binding Proteins Represses Translation in Late Development

1999 • 685 citations

Influences of mRNA secondary structure on initiation by eukaryotic ribosomes.

1986 • 684 citations

Cocrystal Structure of the Messenger RNA 5′ Cap-Binding Protein (eIF4E) Bound to 7-methyl-GDP

1997 • 660 citations

Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides

1994 • 584 citations

Three abundance classes in HeLa cell messenger RNA

1974 • 563 citations

TAP, the Human Homolog of Mex67p, Mediates CTE-Dependent RNA Export from the Nucleus

1998 • 557 citations

Normalization and subtraction: two approaches to facilitate gene discovery.

1996 • 542 citations

5′-Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated carboxy-terminal domain of RNA polymerase II

1997 • 540 citations

Effects of Intercistronic Length on the Efficiency of Reinitiation by Eucaryotic Ribosomes

1987 • 540 citations

Regulation by HIV Rev depends upon recognition of splice sites

1989 • 517 citations

Deadenylation of the unstable mRNA encoded by the yeast MFA2 gene leads to decapping followed by 5'-->3' digestion of the transcript.

1994 • 511 citations

Amplification and characterization of a β-globin gene synthesized in vitro

1976 • 495 citations

Pre-steady-state kinetic analysis of processive DNA replication including complete characterization of an exonuclease-deficient mutant

1991 • 495 citations

A non-AUG translational initiation in c-myc exon 1 generates an N-terminally distinct protein whose synthesis is disrupted in Burkitt's lymphomas

1988 • 481 citations

High molecular mass forms of basic fibroblast growth factor are initiated by alternative CUG codons.

1989 • 469 citations

Experimental annotation of the human genome using microarray technology

2001 • 446 citations

A small element from the Mason-Pfizer monkey virusgenome makes human immunodeficiency virus type 1 expression and replicationRev-independent.

1994 • 425 citations

Construction and characterization of a normalized cDNA library.

1994 • 411 citations

First Glimpses at Structure-function Relationships of the Nucleocapsid Protein of Retroviruses

1995 • 402 citations

The rev gene product of the human immunodeficiency virus affects envelope-specific RNA localization

1989 • 400 citations

Using <tt>GeneWise</tt> in the Drosophila Annotation Experiment

2000 • 368 citations

The rev (trs/art) protein of human immunodeficiency virus type 1 affects viral mRNA and protein expression via a cis-acting sequence in the env region

1989 • 359 citations

lyl-1, a novel gene altered by chromosomal translocation in T cell leukemia, codes for a protein with a helix-loop-helix DNA binding motif

1989 • 358 citations

CUUCGG hairpins: extraordinarily stable RNA secondary structures associated with various biochemical processes.

1988 • 357 citations

Enzymatic in vitro synthesis of globin genes

1976 • 343 citations

Normalization strategies for cDNA microarrays

2000 • 342 citations

Estimate of human gene number provided by genome-wide analysis using Tetraodon nigroviridis DNA sequence

2000 • 324 citations

Oligodeoxyribonucleotide ligation to single-stranded cDNAs: a new tool for cloning 5′ ends of mRNAs and for constructing cDNA libraries byin vitroamplification

1991 • 312 citations

The pim-1 oncogene encodes two related protein-serine/threonine kinases by alternative initiation at AUG and CUG.

1991 • 303 citations

High-Efficiency Full-Length cDNA Cloning by Biotinylated CAP Trapper

1996 • 303 citations

Subcellular fate of the lnt-2 oncoprotein is determined by choice of initiation codon

1990 • 293 citations

A thermodynamic study of unusually stable RNA and DNA hairpins

1991 • 288 citations

PCR-based cDNA library construction: general cDNA libraries at the level of a few cells

1989 • 285 citations

Normalization and Subtraction of Cap-Trapper-Selected cDNAs to Prepare Full-Length cDNA Libraries for Rapid Discovery of New Genes

2000 • 283 citations

Integrated Cell Isolation and Polymerase Chain Reaction Analysis Using Silicon Microfilter Chambers

1998 • 279 citations

Effects of intercistronic length on the efficiency of reinitiation by eucaryotic ribosomes.

1987 • 277 citations

Cap-Binding Proteins of Eukaryotic Messenger RNA: Functions in Initiation and Control of Translation

1988 • 266 citations

The serum-inducible mouse gene Krox-24 encodes a sequence-specific transcriptional activator.

1990 • 256 citations

DNA strand exchange and selective DNA annealing promoted by the human immunodeficiency virus type 1 nucleocapsid protein

1994 • 246 citations

Human immunodeficiency virus 1 reverse transcriptase

1989 • 244 citations

The constitutive transport element (CTE) of Mason-Pfizer monkey virus (MPMV) accesses a cellular mRNA export pathway

1997 • 236 citations

Thermostabilization and thermoactivation of thermolabile enzymes by trehalose and its application for the synthesis of full length cDNA

1998 • 235 citations

An RNA chaperone activity of non-specific RNA binding proteins in hammerhead ribozyme catalysis.

1994 • 234 citations

High copy number of the pUC plasmid results from a Rom/Rop‐suppressible point mutation in RNA II

1992 • 228 citations

Domain structure of the Moloney murine leukemia virus reverse transcriptase: mutational analysis and separate expression of the DNA polymerase and RNase H activities.

1988 • 206 citations

Fidelity of HIV-1 reverse transcriptase copying RNA in vitro

1992 • 205 citations

Fidelity of two retroviral reverse transcriptases during DNA-dependent DNA synthesis in vitro.

1989 • 189 citations

Purification and characterization of an avian myeloblastosis and human immunodeficiency virus reverse transcriptase inhibitor, sulfated polysaccharides extracted from sea algae

1987 • 188 citations

Human immunodeficiency virus nucleocapsid protein accelerates strand transfer of the terminally redundant sequences involved in reverse transcription.

1994 • 188 citations

Ligation-anchored PCR: a simple amplification technique with single-sided specificity.

1992 • 181 citations

Cloning and Functional Analysis of cDNAs with Open Reading Frames for 300 Previously Undefined Genes Expressed in CD34+ Hematopoietic Stem/Progenitor Cells

2000 • 178 citations

Interactions between HIV-1 nucleocapsid protein and viral DNA may have important functions in the viral life cycle

1993 • 177 citations

Studies on the mechanism of human immunodeficiency virus reverse transcriptase. Steady-state kinetics, processivity, and polynucleotide inhibition.

1988 • 167 citations

Construction of a uniform-abundance (normalized) cDNA library.

1991 • 164 citations

Insertion of a rabbit β-globin gene sequence into an E.coli plasmid

1975 • 164 citations

A novel hepatitis B virus (HBV) genetic element with Rev response element-like properties that is essential for expression of HBV gene products.

1993 • 152 citations

Recombinant human immunodeficiency virus type 1 nucleocapsid (NCp7) protein unwinds tRNA.

1992 • 150 citations

Role of the Hepatitis B Virus Posttranscriptional Regulatory Element in Export of Intronless Transcripts

1995 • 149 citations

HnRNP L binds a cis-acting RNA sequence element that enables intron-dependent gene expression.

1995 • 149 citations

Bacterial fitness and plasmid loss: the importance of culture conditions and plasmid size

1998 • 148 citations

A Non-AUG Translational Initiation Event Generates Novel WT1 Isoforms

1996 • 148 citations

Molecular Cloning, Genomic Organization, and Functional Expression of Na+/H+ Exchanger Isoform 5 (NHE5) from Human Brain

1999 • 147 citations

Facilitation of hammerhead ribozyme catalysis by the nucleocapsid protein of HIV-1 and the heterogeneous nuclear ribonucleoprotein A1.

1994 • 146 citations

Discontinuous transcription or RNA processing of vaccinia virus late messengers results in a 5′ poly(A) leader

1987 • 144 citations

Analysis of the nucleic acid annealing activites of nucleocapsid protein from HIV-1

1995 • 144 citations

Statistical Analysis of the 5′ Untranslated Region of Human mRNA Using “Oligo-Capped” cDNA Libraries

2000 • 141 citations

A Human cDNA Library for High-Throughput Protein Expression Screening

2000 • 140 citations

Complexity of cytoplasmic polyadenylated and nonpolyadenylated rat brain ribonucleic acids

1979 • 137 citations

Fine structure analysis of the WT1 gene in sporadic Wilms tumors.

1994 • 135 citations

Human immunodeficiency virus type 1 nucleocapsid protein reduces reverse transcriptase pausing at a secondary structure near the murine leukemia virus polypurine tract

1996 • 135 citations

Complex population of nonpolyadenylated messenger RNA in mouse brain

1979 • 134 citations

A monoclonal antibody against 2,2,7‐trimethylguanosine that reacts with intact, class U, small nuclear ribonucleoproteins as well as with 7‐methylguanosine‐capped RNAs

1987 • 133 citations

An ‘equalized cDNA library’ by the reassociation of short double-stranded cDNAs

1990 • 133 citations

Enhanced discrimination of single nucleotide polymorphisms by artificial mismatch hybridization

1997 • 131 citations

The intronic region of an incompletely spliced gp100 gene transcript encodes an epitope recognized by melanoma-reactive tumor-infiltrating lymphocytes.

1997 • 121 citations

Reverse Transcriptase

1997 • 117 citations

RAR-beta 4, a retinoic acid receptor isoform is generated from RAR-beta 2 by alternative splicing and usage of a CUG initiator codon.

1992 • 115 citations

Illimaquinone, a selective inhibitor of the RNase H activity of human immunodeficiency virus type 1 reverse transcriptase

1990 • 114 citations

Intronless mRNA transport elements may affect multiple steps of pre-mRNA processing

1999 • 113 citations

Expression of the hepatocyte Na+/bile acid cotransporter in Xenopus laevis oocytes.

1990 • 106 citations

mRNA isolation in a microfluidic device for eventual integration of cDNA library construction

2000 • 105 citations

CapSelect: a highly sensitive method for 5' CAP-dependent enrichment of full-length cDNA in PCR-mediated analysis of mRNAs

1999 • 101 citations

The immediate downstream codon strongly influences the efficiency of utilization of eukaryotic translation initiation codons.

1994 • 101 citations

A general method for cloning eukaryotic structural gene sequences.

1976 • 100 citations

Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning

2000 • 100 citations

CapSelect: A highly sensitive method for 5' CAP-dependent enrichment of full-length cDNA in PCR-mediated analysis of mRNAs

1999 • 99 citations

Mechanisms of translational control in early development

1996 • 95 citations

Fidelity of HIV-1 Reverse Transcriptase Copying a Hypervariable Region of the HIV-1 env Gene

1994 • 91 citations

An Algorithm for Clustering cDNA Fingerprints

2000 • 89 citations

The ltk receptor tyrosine kinase is expressed in pre-B lymphocytes and cerebral neurons and uses a non-AUG translational initiator.

1990 • 88 citations

Positions +5 and +6 can be major determinants of the efficiency of non-AUG initiation codons for protein synthesis.

1994 • 88 citations

Fidelity of the RNA-dependent DNA synthesis exhibited by the reverse transcriptases of human immunodeficiency virus types 1 and 2 and of murine leukemia virus: mispair extension frequencies

1992 • 86 citations

Retroviral nucleocapsid proteins possess potent nucleic acid strand renaturation activity

1993 • 84 citations

Effect of the cap structure on pre-mRNA splicing in Xenopus oocyte nuclei.

1989 • 83 citations

Cell-free translation analysis of messenger RNA in echinoderm and amphibian early development

1977 • 80 citations

An Efficient Strategy To Isolate Full-Length cDNAs Based on an mRNA Cap Retention Procedure (CAPture)

1995 • 79 citations

LAR tyrosine phosphatase receptor: alternative splicing is preferential to the nervous system, coordinated with cell growth and generates novel isoforms containing extensive CAG repeats.

1995 • 79 citations

Fidelity of human immunodeficiency virus type I reverse transcriptase in copying natural RNA

1992 • 78 citations

Post-transcriptional Regulation of Erythropoietin mRNA Stability by Erythropoietin mRNA-binding Protein

1997 • 76 citations

Stepwise biosynthesis in vitro of globin genes from globin mRNA by DNA polymerase of avian myeloblastosis virus.

1976 • 72 citations

Conditional mutants of the yeast mRNA capping enzyme show that the cap enhances, but is not required for, mRNA splicing.

1996 • 69 citations

[21] Isolation of cytoplasmic RNA: Ribonucleoside-vanadyl complexes

1987 • 66 citations

Characterization of cDNA and Genomic Clones Encoding Human Myelin Oligodendrocyte Glycoprotein

1995 • 64 citations

Comparative studies of the inhibitory properties of antibiotics on human immunodeficiency virus and avian myeloblastosis virus reverse transcriptases and cellular DNA polymerases.

1989 • 64 citations

Polymer-stimulated ligation: enhanced ligation of oligo- and polynucleotides by T4 RNA ligase in polymer solutions

1984 • 61 citations

Cell biology of paget's disease

1999 • 59 citations

Highly Conserved RNA Sequences That Are Sensors of Environmental Stress

1998 • 59 citations

Novel variant of the P2X2 ATP receptor from the guinea pig organ of Corti

1998 • 58 citations

Fidelity of the reverse transcriptase of human immunodeficiency virus type 2

1992 • 57 citations

Novel metabolism of several beta zero-thalassemic beta-globin mRNAs in the erythroid tissues of transgenic mice.

1989 • 56 citations

Studies of the Murine Homolog of the Multiple Endocrine Neoplasia Type 1 (MEN1) Gene, men1

1999 • 56 citations

Studies on reverse transcriptase of RNA tumor viruses. I. Localization of thermolabile DNA polymerase and RNase H activities on one polypeptide

1975 • 55 citations

Characterization of the Complete Genomic Structure of the Human WNT-5A Gene, Functional Analysis of its Promoter, Chromosomal Mapping, and Expression in Early Human Embryogenesis

1995 • 54 citations

[1] Isolation of RNA

1989 • 53 citations

Studies of Nucleotide Sequences in Tobacco Mosaic Virus Ribonucleic Acid. III. Periodate Oxidation and Semicarbazone Formation^*

1966 • 52 citations

HEED, the Product of the Human Homolog of the Murineeed Gene, Binds to the Matrix Protein of HIV-1

1999 • 51 citations

A novel transforming growth factor-β₁responsive cytoplasmic frans-acting factor binds selectively to the 3′-untranslated region of mammalian ribonucleotide reductase R2 mRNA: role in message stability

1993 • 51 citations

X chromosome inactivation of the human TIMP gene

1990 • 51 citations

Systematic screening of an arrayed cDNA library by PCR.

1995 • 49 citations

Discriminatory interaction of purified eukaryotic initiation factors 4F plus 4A with the 5' ends of reovirus messenger RNAs.

1988 • 47 citations

Second-strand cDNA synthesis withE.coliDNA polymerase I and RNase H: the fate of information at the mRNA 5' terminus and the effect ofE.coliDNA ligase

1988 • 46 citations

Amino Acid Requirements of the Nucleocapsid Protein of HIV-1 for Increasing Catalytic Activity of a Ki-ras Ribozyme in Vitro

1994 • 46 citations

Tissue Gene Expression Analysis Using Arrayed Normalized cDNA Libraries

2000 • 45 citations

The RNA-dependent DNA polymerase of avian sarcoma virus B77. Binding of viral and nonviral ribonucleic acids to the alpha, beta2, and alphabeta forms of the enzyme.

1977 • 44 citations

Cis-acting sequences affecting the length of the poly(A) head of vaccinia virus late transcripts

1988 • 42 citations

Isolation and characterization of trout testis protamine mRNAs lacking Poly(A)

1977 • 41 citations

Comparative evaluation of 5′-end-sequence quality of clones in CAP trapper and other full-length-cDNA libraries

2001 • 41 citations

Translational regulation of the heat shock response

1994 • 39 citations

Screening poly(dA/dT) ⁻ cDNAs for gene identification

2000 • 38 citations

Visualization of the interaction of a regulatory protein with RNA in vivo.

1993 • 38 citations

[19] Preparation and characterization of RNA: Overview

1987 • 31 citations

Selective modification of cytidine residue in ribonucleic acid by semicarbazide

1964 • 29 citations

Absence of poly(A) in a large part of newly synthesized casein mRNAs

1976 • 28 citations

Use of conversion adaptors to clone antigen genes in λgt11

1987 • 27 citations

Relationship between nuclear and cytoplasmic poly(adenylic acid).

1975 • 26 citations

Identification and Purification of Cellular Proteins That Specifically Interact with the RNA Constitutive Transport Elements from Retrovirus D

1997 • 26 citations

The Unique Cytoplasmic Domain of the Human Integrin Variant β4E Is Produced by Partial Retention of Intronic Sequences

1997 • 26 citations

Intron Retention May Regulate Expression of Epstein-Barr Virus Nuclear Antigen 3 Family Genes

1999 • 25 citations

Identification of two cis-acting elements that independently regulate the length of poly(A) on Xenopus albumin pre-mRNA

1998 • 24 citations

Hydrolytic cleavage of RNA catalyzed by metal ion complexes.

1996 • 22 citations

Monoclonal Antibody-Mediated Inhibition of RNA Binding and Annealing Activities of HIV Type 1 Nucleocapsid Protein

1994 • 19 citations

Characterization of the 5′‐flanking promoter region of the rat somatostatin receptor subtype 3 gene

1998 • 17 citations

A novel isoform of human membrane cofactor protein (CD46) mRNA generated by intron retention

1998 • 15 citations

Expression of a rat liver phosphatidylcholine translocator in Xenopus laevis oocytes

1996 • 15 citations

Generation of Full-Length cDNA Libraries Enriched for Differentially Expressed Genes for Functional Genomics

2001 • 11 citations

Efficient Second-Strand cDNA Synthesis Using T7 DNA Polymerase

1994 • 10 citations

The amphiphilic properties of novenamines determine their activity as inhibitors of HIV-1 RNase H

1996 • 10 citations

Extensive homology between poly(A)-containing mRNA and purified nominal poly(A)-lacking mRNA in mouse kidney.

1981 • 8 citations

Periodate oxidation of pseudouridine

1965 • 7 citations

Novel strategy for synthesis sf full-length double-stranded cDNA transcripts without dC-BG tails

1991 • 5 citations

T7 DNA Polymerase Requires Unusual Reaction Conditions for Blunt-Ending Activity

1994 • 4 citations

Recovery of biologically functional messenger RNA from agarose gels by passive elution

1986 • 3 citations