Efficient Second-Strand cDNA Synthesis Using T7 DNA Polymerase

Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.

1988 • 4,669 citations

A simple and very efficient method for generating cDNA libraries

1983 • 4,606 citations

Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase

1988 • 4,259 citations

High-efficiency cloning of full-length cDNA.

1982 • 763 citations

The human c-myc oncogene: Structural consequences of translocation into the igh locus in Burkitt lymphoma

1983 • 732 citations

Polymerase Chain Reaction with Single-Sided Specificity: Analysis of T Cell Receptor δ Chain

1989 • 640 citations

DNA recombination during PCR

1990 • 583 citations

DNA damage promotes jumping between templates during enzymatic amplification.

1990 • 510 citations

Amplification and characterization of a β-globin gene synthesized in vitro

1976 • 495 citations

Pre-steady-state kinetic analysis of processive DNA replication including complete characterization of an exonuclease-deficient mutant

1991 • 495 citations

Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7.

1987 • 399 citations

Enzymatic in vitro synthesis of globin genes

1976 • 343 citations

PCR-based cDNA library construction: general cDNA libraries at the level of a few cells

1989 • 285 citations

Selective Inactivation of the Exonuclease Activity of Bacteriophage T7 DNA Polymerase by in Vitro Mutagenesis

1989 • 258 citations

One-sided polymerase chain reaction: the amplification of cDNA.

1989 • 241 citations

Fidelity of DNA synthesis by the Thermococcus litoralis DNA polymerase—an extremely heat stable enzyme with proofreading activity

1991 • 227 citations

[33] First-strand cDNA synthesis primed with oligo(dT)

1987 • 220 citations

Kinetic partitioning between the exonuclease and polymerase sites in DNA error correction

1991 • 208 citations

Nucleotide sequence of the human c-myc locus: provocative open reading frame within the first exon.

1984 • 207 citations

Ligation-anchored PCR: a simple amplification technique with single-sided specificity.

1992 • 181 citations

An improved procedure for utilizing terminal transferase to add homopolymers to the 3′ termini of DNA

1981 • 176 citations

Insertion of a rabbit β-globin gene sequence into an E.coli plasmid

1975 • 164 citations

A general method for cloning eukaryotic structural gene sequences.

1976 • 100 citations

[32] Preparation of cDNA and the generation of cDNA libraries: Overview

1987 • 94 citations

Two forms of the DNA polymerase of bacteriophage T7.

1983 • 81 citations

Stepwise biosynthesis in vitro of globin genes from globin mRNA by DNA polymerase of avian myeloblastosis virus.

1976 • 72 citations

Increased level of amplification of the c-myc oncogene in tumors induced in nude mice by a human breast carcinoma cell line.

1985 • 66 citations

Disruption of phase during PCR amplification and cloning of heterozygous target sequences

1990 • 52 citations

High c-myc amplification level contributes to the tumorigenic phenotype of the human breast carcinoma cell line SW 613-S.

1988 • 36 citations

An efficient strategy for cloning 5′ extremities of rare transcripts permits isolation of multiple 5′-untransiated regions of rat tryptophan hydroxylase mRNA

1989 • 33 citations

DNA nicking favors PCR recombination

1991 • 33 citations

The human breast carcinoma cell line SW 613-S: an experimental system to study tumor heterogeneity in relation to c-myc amplification, growth factor production and other markers (review).

1989 • 30 citations

Novel strategy for synthesis sf full-length double-stranded cDNA transcripts without dC-BG tails

1991 • 5 citations

T7 DNA Polymerase Requires Unusual Reaction Conditions for Blunt-Ending Activity

1994 • 4 citations