PCR-based cDNA library construction: general cDNA libraries at the level of a few cells

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease

1979 • 22,482 citations

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

1988 • 17,059 citations

Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia

1985 • 9,128 citations

Genomic sequencing.

1984 • 8,343 citations

Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.

1988 • 4,669 citations

Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing

1984 • 4,231 citations

A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase

1975 • 2,622 citations

Single-stranded DNA ‘blue’ T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineering

1986 • 816 citations

Heavy chain variable region contribution to the NPb family of antibodies: somatic mutation evident in a γ2a variable region

1981 • 677 citations

Two mRNAs with different 3′ ends encode membrane-bound and secreted forms of immunoglobulin μ chain

1980 • 659 citations

Polymerase Chain Reaction with Single-Sided Specificity: Analysis of T Cell Receptor δ Chain

1989 • 640 citations

Sequences of mouse immunoglobulin light chain genes before and after somatic changes

1978 • 495 citations

Isolation of full-length putative rat lysophospholipase cDNA using improved methods for mRNA isolation and cDNA cloning

1987 • 441 citations

Alkaline transfer of DNA to plastic membrane

1984 • 285 citations

[33] First-strand cDNA synthesis primed with oligo(dT)

1987 • 220 citations

A novel 34-kd protein co-isolated with the IgM molecule in surface IgM-expressing cells.

1988 • 209 citations

Activation of V kappa gene rearrangement in pre-B cells follows the expression of membrane-bound immunoglobulin heavy chains.

1987 • 187 citations

[32] Preparation of cDNA and the generation of cDNA libraries: Overview

1987 • 94 citations

DNA sequence of the mouse H-2Dd transplantation antigen gene.

1985 • 93 citations

A cDNA clone containing the entire coding sequence of a mouse H-2Kd histocompatibility antigen

1983 • 84 citations

Dual expression of λ genes in the MOPC-315 plasmacytoma

1981 • 78 citations

cDNA cloning of the immunoglobulin heavy chain binding protein.

1988 • 74 citations

[20] Synthesis of ds-cDNA involving addition of dCMP tails to allow cloning of 5′-terminal mRNA sequences

1983 • 35 citations

[59] Sequencing of RNA transcripts synthesized in vitro from plasmids containing bacteriophage promoters

1987 • 16 citations

Rapid synthesis of cDNA for cloning into lambda vectors

1986 • 12 citations

[Amplification of total cDNA in vitro].

1988 • 1 citations