The importance of the proteasome and subsequent proteolytic steps in the generation of antigenic peptides

Inhibitors of the proteasome block the degradation of most cell proteins and the generation of peptides presented on MHC class I molecules

1994 • 2,480 citations

DEGRADATION OF CELL PROTEINS AND THE GENERATION OF MHC CLASS I-PRESENTED PEPTIDES

1999 • 898 citations

Peptides Naturally Presented by MHC Class I Molecules

1993 • 803 citations

A gated channel into the proteasome core particle.

2000 • 791 citations

Potent and selective inhibitors of the proteasome: Dipeptidyl boronic acids

1998 • 674 citations

Heat shock proteins transfer peptides during antigen processing and CTL priming

1994 • 580 citations

γ-Interferon and expression of MHC genes regulate peptide hydrolysis by proteasomes

1993 • 572 citations

The Sizes of Peptides Generated from Protein by Mammalian 26 and 20 S Proteasomes

1999 • 565 citations

Aminopeptidases: structure and function

1993 • 544 citations

MHC Class I Expression in Mice Lacking the Proteasome Subunit LMP-7

1994 • 520 citations

An IFN-γ–induced aminopeptidase in the ER, ERAP1, trims precursors to MHC class I–presented peptides

2002 • 518 citations

Structural basis for the activation of 20S proteasomes by 11S regulators

2000 • 503 citations

MHC-linked LMP gene products specifically alter peptidase activities of the proteasome

1993 • 453 citations

Altered peptidase and viral-specific T cell response in LMP2 mutant mice

1994 • 450 citations

Covalent modification of the active site threonine of proteasomal β subunits and theEscherichia colihomolog HslV by a new class of inhibitors

1997 • 441 citations

Identification, purification, and characterization of a protein activator (PA28) of the 20 S proteasome (macropain).

1992 • 439 citations

Cellular peptide composition governed by major histocompatibility complex class I molecules

1990 • 438 citations

A proteasome-related gene between the two ABC transporter loci in the class II region of the human MHC

1991 • 427 citations

The Axial Channel of the Proteasome Core Particle Is Gated by the Rpt2 ATPase and Controls Both Substrate Entry and Product Release

2001 • 413 citations

Lactacystin and clasto-Lactacystin β-Lactone Modify Multiple Proteasome β-Subunits and Inhibit Intracellular Protein Degradation and Major Histocompatibility Complex Class I Antigen Presentation

1997 • 388 citations

Purification of an 11 S regulator of the multicatalytic protease.

1992 • 384 citations

A Giant Protease with Potential to Substitute for Some Functions of the Proteasome

1999 • 377 citations

Interferon-γ Induces Different Subunit Organizations and Functional Diversity of Proteasomes1

1994 • 371 citations

Hybrid Proteasomes

2000 • 342 citations

A role for the proteasome regulator PA28α in antigen presentation

1996 • 341 citations

Defective presentation to class I-restricted cytotoxic T lymphocytes in vaccinia-infected cells is overcome by enhanced degradation of antigen.

1988 • 331 citations

26S proteasomes and immunoproteasomes produce mainly N-extended versions of an antigenic peptide

2001 • 322 citations

A role for the ubiquitin-dependent proteolytic pathway in MHC class l-restricted antigen presentation

1993 • 317 citations

Peptidase activities of proteasomes are differentially regulated by the major histocompatibility complex-encoded genes for LMP2 and LMP7.

1994 • 314 citations

Interferon-γ Can Stimulate Post-proteasomal Trimming of the N Terminus of an Antigenic Peptide by Inducing Leucine Aminopeptidase

1998 • 297 citations

Importance of Peptide Amino and Carboxyl Termini to the Stability of MHC Class I Molecules

1994 • 294 citations

A proteolytic system that compensates for loss of proteasome function

1998 • 271 citations

A soluble metalloendopeptidase from rat brain

1983 • 270 citations

DNA immunization: ubiquitination of a viral protein enhances cytotoxic T-lymphocyte induction and antiviral protection but abrogates antibody induction

1997 • 269 citations

Two distinct proteolytic processes in the generation of a major histocompatibility complex class I-presented peptide

1997 • 267 citations

Subunit of the '20S' proteasome (multicatalytic proteinase) encoded by the major histocompatibility complex

1991 • 262 citations

The proteasome activator 11 S REG (PA28) and Class I antigen presentation

1999 • 262 citations

Homology of proteasome subunits to a major histocompatibility complex-linked LMP gene

1991 • 256 citations

Two new proteases in the MHC class I processing pathway

2000 • 253 citations

Contribution of proteasome-mediated proteolysis to the hierarchy of epitopes presented by major histocompatibility complex class I molecules

1995 • 225 citations

Simultaneous binding of PA28 and PA700 activators to 20 S proteasomes

1998 • 219 citations

Peptide size selection by the major histocompatibility complex-encoded peptide transporter.

1994 • 212 citations

Properties of the hybrid form of the 26S proteasome containing both 19S and PA28 complexes

2002 • 209 citations

Range of Sizes of Peptide Products Generated during Degradation of Different Proteins by Archaeal Proteasomes

1998 • 208 citations

Peptide length and sequence specificity of the mouse TAP1/TAP2 translocator.

1994 • 207 citations

Newly identified pair of proteasomal subunits regulated reciprocally by interferon gamma.

1996 • 195 citations

Structure of the proteasome activator REGα (PA28α)

1997 • 187 citations

ER aminopeptidases generate a unique pool of peptides for MHC class I molecules

2001 • 184 citations

Impaired Immunoproteasome Assembly and Immune Responses in PA28 ^−/− Mice

1999 • 177 citations

Targeting of HIV-1 Antigens for Rapid Intracellular Degradation Enhances Cytotoxic T Lymphocyte (CTL) Recognition and the Induction of De Novo CTL Responses In Vivo After Immunization

1997 • 176 citations

The mouse and human genes encoding the recognition component of the N-end rule pathway

1998 • 175 citations

Trimming of antigenic peptides in an early secretory compartment.

1994 • 169 citations

Proteasome Subunits X and Y Alter Peptidase Activities in Opposite Ways to the Interferon-γ-induced Subunits LMP2 and LMP7

1996 • 164 citations

Characterization of Recombinant REGα, REGβ, and REGγ Proteasome Activators

1997 • 164 citations

Processing of major histocompatibility class I-restricted antigens in the endoplasmic reticulum.

1995 • 163 citations

Puromycin-sensitive Aminopeptidase

1995 • 161 citations

Immunoproteasome assembly and antigen presentation in mice lacking both PA28alpha and PA28beta

2001 • 155 citations

Major differences in transporter associated with antigen presentation (TAP)-dependent translocation of MHC class I-presentable peptides and the effect of flanking sequences.

1995 • 155 citations

Major Histocompatibility Complex Class I-presented Antigenic Peptides Are Degraded in Cytosolic Extracts Primarily by Thimet Oligopeptidase

2001 • 147 citations

Tripeptidyl peptidases: enzymes that count

1999 • 145 citations

Interferon-γ inducible exchanges of 20S proteasome active site subunits: Why?

2001 • 145 citations

Distinct Proteolytic Processes Generate the C and N Termini of MHC Class I-Binding Peptides

1999 • 144 citations

Rate of antigen degradation by the ubiquitin-proteasome pathway influences MHC class I presentation.

1995 • 140 citations

The proteasome‐specific inhibitor lactacystin blocks presentation of cytotoxic T lymphocyte epitopes in human and murine cells

1997 • 129 citations

Genes encoded in the major histocompatibility complex affecting the generation of peptides for TAP transport

1995 • 129 citations

Molecular properties of the proteasome activator PA28 family proteins and γ‐interferon regulation

1997 • 126 citations

Proteasomes Can Either Generate or Destroy MHC Class I Epitopes: Evidence for Nonproteasomal Epitope Generation in the Cytosol

1998 • 124 citations

Discrete Proteolytic Intermediates in the MHC Class I Antigen Processing Pathway and MHC I–Dependent Peptide Trimming in the ER

1999 • 123 citations

Overexpression of the Proteasome Subunits LMP2, LMP7, and MECL-1, But Not PA28α/β, Enhances the Presentation of an Immunodominant Lymphocytic Choriomeningitis Virus T Cell Epitope

2000 • 121 citations

The Role of Tricorn Protease and Its Aminopeptidase-Interacting Factors in Cellular Protein Degradation

1998 • 119 citations

The cleavage preference of the proteasome governs the yield of antigenic peptides.

1995 • 117 citations

The Human 26 S and 20 S Proteasomes Generate Overlapping but Different Sets of Peptide Fragments from a Model Protein Substrate

2000 • 116 citations

Proteasome Activator 11S REG or PA28: Recombinant REGα/REGβ Hetero-oligomers Are Heptamers¹

1999 • 110 citations

Intracellular targeting of the proteasome

2000 • 109 citations

The proteolytic fragments generated by vertebrate proteasomes: structural relationships to major histocompatibility complex class I binding peptides.

1996 • 108 citations

Differential Influence on Cytotoxic T Lymphocyte Epitope Presentation by Controlled Expression of Either Proteasome Immunosubunits or Pa28

2000 • 106 citations

The Selective Proteasome Inhibitors Lactacystin and Epoxomicin Can Be Used to Either Up- or Down-Regulate Antigen Presentation at Nontoxic Doses

2000 • 106 citations

Analysis of the fine specificity of rat, mouse and human TAP peptide transporters

1995 • 102 citations

[32] Thimet oligopeptidase and oligopeptidase M or neurolysin

1995 • 100 citations

Novel dipeptide aldehydes are proteasome inhibitors and block the MHC-I antigen-processing pathway.

1995 • 99 citations

LMP2+ proteasomes are required for the presentation of specific antigens to cytotoxic T lymphocytes

1995 • 97 citations

Effects of Major‐Histocompatibility‐Complex‐Encoded Subunits on the Peptidase and Proteolytic Activities of Human 20S Proteasomes

1996 • 95 citations

Characterization of the Proteasome Regulator PA28

1996 • 93 citations

The proteasome activator 11 S REG (PA28) and Class I antigen presentation

2000 • 93 citations

Human Transporters Associated with Antigen Processing (Taps) Select Epitope Precursor Peptides for Processing in the Endoplasmic Reticulum and Presentation to T Cells

1999 • 89 citations

Cytotoxic T Lymphocyte Epitopes of HIV-1 Nef

2000 • 80 citations

Generation of the vesicular stomatitis virus nucleoprotein cytotoxic T lymphocyte epitope requires proteasome-dependent and -independent proteolytic activities

1998 • 78 citations

The specificity of proteasomes: impact on MHC class I processing and presentation of antigens

1999 • 76 citations

Crystal structure of human bleomycin hydrolase, a self-compartmentalizing cysteine protease

1999 • 71 citations

Purification of rabbit brain endooligopeptidases and preparation of antienzyme antibodies

1981 • 70 citations

20S proteasome from LMP7 knock out mice reveals altered proteolytic activities and cleavage site preferences

1996 • 66 citations

Cutting Edge: Induction of Enhanced CTL-Dependent Protective Immunity In Vivo by N-End Rule Targeting of a Model Tumor Antigen

1999 • 66 citations

Thimet Oligopeptidase and the Stability of MHC Class I Epitopes in Macrophage Cytosol

1999 • 60 citations

Characterization of peptidyl boronic acid inhibitors of mammalian 20 S and 26 S proteasomes and their inhibition of proteasomes in cultured cells

2000 • 54 citations

Chemical denaturation and modification of ovalbumin alters its dependence on ubiquitin conjugation for class I antigen presentation.

1996 • 50 citations

A Role for a Novel Luminal Endoplasmic Reticulum Aminopeptidase in Final Trimming of 26 S Proteasome-generated Major Histocompatability Complex Class I Antigenic Peptides

2001 • 45 citations

The role of MHC class I molecules in the generation of endogenous peptide/MHC complexes.

1995 • 41 citations

Newly Identified Pair of Proteasomal Subunits Regulated Reciprocally by Interferon γ

1997 • 39 citations

Peptide intermediates in the degradation of cellular proteins. Bestatin permits their accumulation in mouse liver in vivo.

1983 • 37 citations

Role of bestatin-Sensitive Exopeptidases in the Intracellular Degradation of Hepatic Proteins

1989 • 29 citations

Biochemical Purification of Distinct Proteasome Subsets

1993 • 20 citations

Production of a Specific Major Histocompatibility Complex Class I-restricted Epitope by Ubiquitin-dependent Degradation of Modified Ovalbumin in Lymphocyte Lysate

1997 • 19 citations

Characterization of peptidyl boronic acid inhibitors of mammalian 20 S and 26 S proteasomes and their inhibition of proteasomes in cultured cells

2000 • 18 citations

Proteasomes generate in vitro a natural peptide of influenza-A nucleoprotein functional in HLA-B27 antigen assembly

1996 • 9 citations