A novel set of spliceosome-associated proteins and the essential splicing factor PSF bind stably to pre-mRNA prior to catalytic step II of the splicing reaction.

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1984 • 6,459 citations

Silver stain for proteins in polyacrylamide gels: A modified procedure with enhanced uniform sensitivity

1981 • 4,191 citations

High resolution two-dimensional electrophoresis of basic as well as acidic proteins

1977 • 3,556 citations

Specific interactions between proteins implicated in splice site selection and regulated alternative splicing

1993 • 741 citations

Normal and mutant human β-globin pre-mRNAs are faithfully and efficiently spliced in vitro

1984 • 679 citations

Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes

1987 • 530 citations

Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs

1986 • 490 citations

Multiple factors including the small nuclear ribonucleoproteins U1 and U2 are necessary for Pre-mRNA splicing in vitro

1985 • 442 citations

Structure of spliceosomal snRNPs and their role in pre-mRNA splicing

1990 • 407 citations

Intron sequences involved in lariat formation during pre-mRNA splicing

1985 • 402 citations

Spliceosome assembly in yeast.

1987 • 402 citations

Identification, purification, and biochemical characterization of U2 small nuclear ribonucleoprotein auxiliary factor.

1989 • 375 citations

Interactions of small nuclear RNA's with precursor messenger RNA during in vitro splicing

1992 • 367 citations

Cloning and characterization of PSF, a novel pre-mRNA splicing factor.

1993 • 359 citations

The U5 and U6 Small Nuclear RNAs as Active Site Components of the Spliceosome

1993 • 349 citations

Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae.

1989 • 340 citations

Characterization and molecular cloning of polypyrimidine tract-binding protein: a component of a complex necessary for pre-mRNA splicing.

1991 • 338 citations

PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome

1991 • 329 citations

The organization of 3' splice-site sequences in mammalian introns.

1989 • 310 citations

An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway.

1991 • 297 citations

A protein associated with small nuclear ribonucleoprotein particles recognizes the 3′ splice site of premessenger RNA

1986 • 283 citations

Mutations in U6 snRNA that Alter Splice Site Specificity: Implications for the Active Site

1993 • 283 citations

Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes

1986 • 283 citations

A protein that specifically recognizes the 3′ splice site of mammalian pre-mRNA introns is associated with a small nuclear ribonucleoprotein

1986 • 274 citations

Site-specific cross-linking of mammalian U5 snRNP to the 5' splice site before the first step of pre-mRNA splicing.

1992 • 273 citations

Scanning from an independently specified branch point defines the 3′ splice site of mammalian introns

1989 • 267 citations

Involvement of U6 snRNA in 5′ Splice Site Selection

1993 • 265 citations

Affinity Chromatography of Splicing Complexes: U2, U5, and U4 + U6 Small Nuclear Ribonucleoprotein Particles in the Spliceosome

1986 • 238 citations

Protein components specifically associated with prespliceosome and spliceosome complexes.

1992 • 218 citations

A novel set of spliceosome-associated proteins and the essential splicing factor PSF bind stably to pre-mRNA prior to catalytic step II of the splicing reaction.

1994 • 217 citations

A conformational rearrangement in the spliceosome is dependent on PRP16 and ATP hydrolysis.

1992 • 205 citations

Spliceosome assembly involves the binding and release of U4 small nuclear ribonucleoprotein.

1988 • 188 citations

A mutational analysis of spliceosome assembly: evidence for splice site collaboration during spliceosome formation.

1987 • 179 citations

A putative ATP binding protein influences the fidelity of branchpoint recognition in yeast splicing

1990 • 176 citations

A functional association between the 5' and 3' splice site is established in the earliest prespliceosome complex (E) in mammals.

1993 • 173 citations

Purification and cDNA cloning of HeLa cell p54^nrb, a nuclear protein with two RNA recognition motifs and extensive homology to human splicing factor PSF andDrosophilaNONA/BJ6

1993 • 163 citations

Evidence for a base-pairing interaction between U6 small nuclear RNA and 5' splice site during the splicing reaction in yeast.

1992 • 161 citations

Interaction of Mammalian Splicing Factor SF3a with U2 snRNP and Relation of Its 60-kD Subunit to Yeast PRP9

1993 • 151 citations

20S small nuclear ribonucleoprotein U5 shows a surprisingly complex protein composition.

1989 • 148 citations

Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with RNA-binding motifs.

1991 • 148 citations

Immunoaffinity purification of a [U4/U6.U5] tri-snRNP from human cells.

1991 • 148 citations

A cold-sensitive mRNA splicing mutant is a member of the RNA helicase gene family.

1991 • 147 citations

An essential splicing factor, SLU7, mediates 3' splice site choice in yeast.

1992 • 144 citations

Association of U6 snRNA with the 5'-splice site region of pre-mRNA in the spliceosome.

1992 • 143 citations

Splicing of yeast nuclear pre-mRNA in vitro requires a functional 40S spliceosome and several extrinsic factors.

1987 • 137 citations

PRP4: a protein of the yeast U4/U6 small nuclear ribonucleoprotein particle.

1989 • 131 citations

Separation of splicing factor SF3 into two components and purification of SF3a activity

1993 • 129 citations

Purification and visualization of native spliceosomes

1988 • 121 citations

A U-rich tract enhances usage of an alternative 3′ splice site in yeast

1991 • 119 citations

Small Nuclear Ribonucleoprotein (RNP) U2 Contains Numerous Additional Proteins and Has a Bipartite RNP Structure Under Splicing Conditions

1993 • 117 citations

Protein composition of mammalian spliceosomes assembled in vitro.

1990 • 112 citations

The yeast PRP6 gene encodes a U4/U6 small nuclear ribonucleoprotein particle (snRNP) protein, and the PRP9 gene encodes a protein required for U2 snRNP binding.

1990 • 106 citations

PRP4 (RNA4) from Saccharomyces cerevisiae: Its Gene Product Is Associated with the U4/U6 Small Nuclear Ribonucleoprotein Particle

1989 • 98 citations

Synthetic lethal mutations suggest interactions between U5 small nuclear RNA and four proteins required for the second step of splicing.

1992 • 98 citations

U4 small nuclear RNA dissociates from a yeast spliceosome and does not participate in the subsequent splicing reaction.

1991 • 97 citations

A trans-acting suppressor restores splicing of a yeast intron with a branch point mutation.

1987 • 95 citations

Domains of yeast U4 spliceosomal RNA required for PRP4 protein binding, snRNP-snRNP interactions, and pre-mRNA splicing in vivo.

1990 • 95 citations

Direct interactions between pre-mRNA and six U2 small nuclear ribonucleoproteins during spliceosome assembly.

1994 • 87 citations

The molecular characterization of PRP6 and PRP9 yeast genes reveals a new cysteine/histidine motif common to several splicing factors.

1990 • 84 citations

Correspondence Between a Mammalian Spliceosome Component and an Essential Yeast Splicing Factor

1993 • 81 citations

Stages in the second reaction of pre-mRNA splicing: the final step is ATP independent.

1993 • 76 citations

Identification of a functional mammalian spliceosome containing unspliced pre-mRNA.

1988 • 75 citations

The mammalian analogue of the yeast PRP8 splicing protein is present in the U4/5/6 small nuclear ribonucleoprotein particle and the spliceosome.

1989 • 74 citations

PRP18, a protein required for the second reaction in pre-mRNA splicing.

1990 • 73 citations

Non-snRNP protein splicing factors

1993 • 71 citations

A U5 Small Nuclear Ribonucleoprotein Particle Protein Involved Only in the Second Step of Pre-mRNA Splicing in Saccharomyces cerevisiae

1993 • 66 citations

PRP4: a Protein of the Yeast U4/U6 Small Nuclear Ribonucleoprotein Particle

1989 • 62 citations

Evidence that the 60-kDa protein of 17S U2 small nuclear ribonucleoprotein is immunologically and functionally related to the yeast PRP9 splicing factor and is required for the efficient formation of prespliceosomes.

1993 • 62 citations

A mammalian protein of 220 kDa binds pre-mRNAs in the spliceosome: a potential homologue of the yeast PRP8 protein.

1990 • 55 citations

U4 Small Nuclear RNA Dissociates from a Yeast Spliceosome and Does Not Participate in the Subsequent Splicing Reaction

1991 • 53 citations

The PRP4 (RNA4) protein of Saccharomyces cerevisiae is associated with the 5' portion of the U4 small nuclear RNA.

1990 • 51 citations

Direct Interactions between Pre-mRNA and Six U2 Small Nuclear Ribonucleoproteins during Spliceosome Assembly

1994 • 51 citations

The yeast PRP8 protein intersacts directly with pre-mRNA

1991 • 37 citations

The Yeast PRP6 Gene Encodes a U4/U6 Small Nuclear Ribonucleoprotein Particle (snRNP) Protein, and the PRP9 Gene Encodes a Protein Required for U2 snRNP Binding

1990 • 35 citations

PRP18, a Protein Required for the Second Reaction in Pre-mRNA Splicing

1990 • 31 citations

Newly identified U4/U6 snRNP-binding proteins by serum autoantibodies from a patient with systemic sclerosis.

1991 • 30 citations

Interaction of the human autoantigen p150 with splicing snRNPs

1993 • 17 citations

The PRP4 (RNA4) Protein of Saccharomyces cerevisiae Is Associated with the 5′ Portion of the U4 Small Nuclear RNA

1990 • 15 citations