Direct Interactions between Pre-mRNA and Six U2 Small Nuclear Ribonucleoproteins during Spliceosome Assembly

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1984 • 6,459 citations

Silver stain for proteins in polyacrylamide gels: A modified procedure with enhanced uniform sensitivity

1981 • 4,191 citations

High resolution two-dimensional electrophoresis of basic as well as acidic proteins

1977 • 3,556 citations

SR proteins: a conserved family of pre-mRNA splicing factors.

1992 • 766 citations

A compensatory base change in U1 snRNA suppresses a 5′ splice site mutation

1986 • 755 citations

Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus

1990 • 723 citations

Biochemical Mechanisms of Constitutive and Regulated Pre-mRNA Splicing

1991 • 708 citations

Normal and mutant human β-globin pre-mRNAs are faithfully and efficiently spliced in vitro

1984 • 679 citations

U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing

1985 • 568 citations

Cloning and domain structure of the mammalian splicing factor U2AF

1992 • 566 citations

Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes

1987 • 530 citations

Recognition of the TACTAAC box during mRNA splicing in yeast involves base pairing to the U2-like snRNA

1987 • 508 citations

A protein factor, ASF, controls cell-specific alternative splicing of SV40 early pre-mRNA in vitro

1990 • 500 citations

The essential pre-mRNA splicing factor SF2 influences 5′ splice site selection by activating proximal sites

1990 • 454 citations

A novel base-pairing interaction between U2 and U6 snRNAs suggests a mechanism for the catalytic activation of the spliceosome

1992 • 438 citations

Messenger RNA Splicing in Yeast: Clues to Why the Spliceosome Is a Ribonucleoprotein

1991 • 433 citations

Intron sequences involved in lariat formation during pre-mRNA splicing

1985 • 402 citations

Immunopurification of heterogeneous nuclear ribonucleoprotein particles reveals an assortment of RNA-binding proteins.

1988 • 399 citations

Identification, purification, and biochemical characterization of U2 small nuclear ribonucleoprotein auxiliary factor.

1989 • 375 citations

U5 snRNA interacts with exon sequences at 5′ and 3′ splice sites

1992 • 368 citations

Interactions of small nuclear RNA's with precursor messenger RNA during in vitro splicing

1992 • 367 citations

A U1 snRNA:pre-mRNA base pairing interaction is required early in yeast spliceosome assembly but does not uniquely define the 5′ cleavage site.

1988 • 364 citations

Cloning and characterization of PSF, a novel pre-mRNA splicing factor.

1993 • 359 citations

Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns.

1989 • 349 citations

Characterization and molecular cloning of polypyrimidine tract-binding protein: a component of a complex necessary for pre-mRNA splicing.

1991 • 338 citations

Mammalian pre-mRNA branch site selection by U2 snRNP involves base pairing.

1989 • 314 citations

5' splice site selection in yeast: genetic alterations in base-pairing with U1 reveal additional requirements.

1988 • 307 citations

An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway.

1991 • 297 citations

A compensatory base change in human U2 snRNA can suppress a branch site mutation.

1989 • 284 citations

Site-specific cross-linking of mammalian U5 snRNP to the 5' splice site before the first step of pre-mRNA splicing.

1992 • 273 citations

An ordered pathway of snRNP binding during mammalian pre-mRNA splicing complex assembly.

1987 • 272 citations

Characterization of cDNAs encoding the polypyrimidine tract-binding protein.

1991 • 257 citations

Biochemical characterization of U2 snRNP auxiliary factor: an essential pre-mRNA splicing factor with a novel intranuclear distribution.

1991 • 255 citations

RNA binding specificity of hnRNP proteins: a subset bind to the 3′ end of introns.

1988 • 251 citations

Affinity Chromatography of Splicing Complexes: U2, U5, and U4 + U6 Small Nuclear Ribonucleoprotein Particles in the Spliceosome

1986 • 238 citations

Protein components specifically associated with prespliceosome and spliceosome complexes.

1992 • 218 citations

Mutations in yeast U5 snRNA alter the specificity of 5′ splice-site cleavage

1991 • 202 citations

The 35-kDa mammalian splicing factor SC35 mediates specific interactions between U1 and U2 small nuclear ribonucleoprotein particles at the 3' splice site.

1992 • 198 citations

Specific and stable intron-factor interactions are established early during in vitro pre-mRNA splicing

1985 • 180 citations

A functional association between the 5' and 3' splice site is established in the earliest prespliceosome complex (E) in mammals.

1993 • 173 citations

Evidence for a base-pairing interaction between U6 small nuclear RNA and 5' splice site during the splicing reaction in yeast.

1992 • 161 citations

Interaction of Mammalian Splicing Factor SF3a with U2 snRNP and Relation of Its 60-kD Subunit to Yeast PRP9

1993 • 151 citations

Regulated Splicing of the Drosophila P Transposable Element Third Intron in Vitro: Somatic Repression

1990 • 149 citations

Immunoaffinity purification of a [U4/U6.U5] tri-snRNP from human cells.

1991 • 148 citations

Association of U6 snRNA with the 5'-splice site region of pre-mRNA in the spliceosome.

1992 • 143 citations

Four yeast spliceosomal proteins (PRP5, PRP9, PRP11, and PRP21) interact to promote U2 snRNP binding to pre-mRNA.

1993 • 142 citations

Separation of splicing factor SF3 into two components and purification of SF3a activity

1993 • 129 citations

Protein composition of mammalian spliceosomes assembled in vitro.

1990 • 112 citations

Mutations at the 3′ splice site can be suppressed by compensatory base changes in U1 snRNA in fission yeast

1992 • 109 citations

Splicing Takes a Holliday

1992 • 109 citations

Three protein factors (SF1, SF3 and U2AF) function in pre-splicing complex formation in addition to snRNPs.

1991 • 104 citations

Conservation between yeast and man of a protein associated with U5 small nuclear ribonucleoprotein

1989 • 101 citations

Correspondence Between a Mammalian Spliceosome Component and an Essential Yeast Splicing Factor

1993 • 81 citations

The U2 small nuclear ribonucleoprotein particle as an autoantigen. Analysis with sera from patients with overlap syndromes.

1988 • 76 citations

The mammalian analogue of the yeast PRP8 splicing protein is present in the U4/5/6 small nuclear ribonucleoprotein particle and the spliceosome.

1989 • 74 citations

Interaction Between PRP11 and SPP91 Yeast Splicing Factors and Characterization of a PRP9-PRP11-SPP91 Complex

1993 • 73 citations

Non-snRNP protein splicing factors

1993 • 71 citations

Evidence that the 60-kDa protein of 17S U2 small nuclear ribonucleoprotein is immunologically and functionally related to the yeast PRP9 splicing factor and is required for the efficient formation of prespliceosomes.

1993 • 62 citations

A mammalian protein of 220 kDa binds pre-mRNAs in the spliceosome: a potential homologue of the yeast PRP8 protein.

1990 • 55 citations

Interactions between PRP9 and SPP91 splicing factors identify a protein complex required in prespliceosome assembly.

1993 • 52 citations

The yeast PRP8 protein intersacts directly with pre-mRNA

1991 • 37 citations

Identification of nuclear proteins that specifically bind to RNAs containing 5' splice sites.

1991 • 19 citations