Protein components specifically associated with prespliceosome and spliceosome complexes.

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1984 • 6,459 citations

Silver stain for proteins in polyacrylamide gels: A modified procedure with enhanced uniform sensitivity

1981 • 4,191 citations

Protein folding in the cell

1992 • 4,138 citations

High resolution two-dimensional electrophoresis of basic as well as acidic proteins

1977 • 3,556 citations

SR proteins: a conserved family of pre-mRNA splicing factors.

1992 • 766 citations

A compensatory base change in U1 snRNA suppresses a 5′ splice site mutation

1986 • 755 citations

Regulation of alternative pre-mRNA splicing by hnRNP A1 and splicing factor SF2

1992 • 724 citations

Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus

1990 • 723 citations

Biochemical Mechanisms of Constitutive and Regulated Pre-mRNA Splicing

1991 • 708 citations

Normal and mutant human β-globin pre-mRNAs are faithfully and efficiently spliced in vitro

1984 • 679 citations

Recognition of cap structure in splicing in vitro of mRNA precursors

1984 • 575 citations

Cloning and domain structure of the mammalian splicing factor U2AF

1992 • 566 citations

Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes

1987 • 530 citations

Recognition of the TACTAAC box during mRNA splicing in yeast involves base pairing to the U2-like snRNA

1987 • 508 citations

A protein factor, ASF, controls cell-specific alternative splicing of SV40 early pre-mRNA in vitro

1990 • 500 citations

The essential pre-mRNA splicing factor SF2 influences 5′ splice site selection by activating proximal sites

1990 • 454 citations

Messenger RNA Splicing in Yeast: Clues to Why the Spliceosome Is a Ribonucleoprotein

1991 • 433 citations

Structure of spliceosomal snRNPs and their role in pre-mRNA splicing

1990 • 407 citations

Immunopurification of heterogeneous nuclear ribonucleoprotein particles reveals an assortment of RNA-binding proteins.

1988 • 399 citations

Identification of functional U1 snRNA-pre-mRNA complexes committed to spliceosome assembly and splicing

1989 • 391 citations

U5 snRNA interacts with exon sequences at 5′ and 3′ splice sites

1992 • 368 citations

A U1 snRNA:pre-mRNA base pairing interaction is required early in yeast spliceosome assembly but does not uniquely define the 5′ cleavage site.

1988 • 364 citations

Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns.

1989 • 349 citations

A conserved family of nuclear phosphoproteins localized to sites of polymerase II transcription.

1991 • 344 citations

Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae.

1989 • 340 citations

Characterization and molecular cloning of polypyrimidine tract-binding protein: a component of a complex necessary for pre-mRNA splicing.

1991 • 338 citations

Mammalian pre-mRNA branch site selection by U2 snRNP involves base pairing.

1989 • 314 citations

5' splice site selection in yeast: genetic alterations in base-pairing with U1 reveal additional requirements.

1988 • 307 citations

An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway.

1991 • 297 citations

A compensatory base change in human U2 snRNA can suppress a branch site mutation.

1989 • 284 citations

A protein associated with small nuclear ribonucleoprotein particles recognizes the 3′ splice site of premessenger RNA

1986 • 283 citations

A protein that specifically recognizes the 3′ splice site of mammalian pre-mRNA introns is associated with a small nuclear ribonucleoprotein

1986 • 274 citations

Scanning from an independently specified branch point defines the 3′ splice site of mammalian introns

1989 • 267 citations

Characterization of cDNAs encoding the polypyrimidine tract-binding protein.

1991 • 257 citations

Biochemical characterization of U2 snRNP auxiliary factor: an essential pre-mRNA splicing factor with a novel intranuclear distribution.

1991 • 255 citations

Affinity Chromatography of Splicing Complexes: U2, U5, and U4 + U6 Small Nuclear Ribonucleoprotein Particles in the Spliceosome

1986 • 238 citations

Two-Dimensional Electrophoresis and Immunological Techniques

1987 • 235 citations

Evidence for the existence of snRNAs U4 and U6 in a single ribonucleoprotein complex and for their association by intermolecular base pairing.

1984 • 213 citations

The 35-kDa mammalian splicing factor SC35 mediates specific interactions between U1 and U2 small nuclear ribonucleoprotein particles at the 3' splice site.

1992 • 198 citations

A monoclonal antibody that recognizes a phosphorylated epitope stains lampbrush chromosome loops and small granules in the amphibian germinal vesicle.

1990 • 193 citations

Anti-RNP monoclonal antibodies derived from a mouse strain with lupus-like autoimmunity.

1982 • 192 citations

Base pairing between U2 and U6 snRNAs is necessary for splicing of a mammalian pre-mRNA

1991 • 172 citations

Evidence for base-pairing between mammalian U2 and U6 small nuclear ribonucleoprotein particles.

1990 • 171 citations

Genetic evidence for base pairing between U2 and U6 snRNA in mammalian mRNA splicing

1991 • 165 citations

20S small nuclear ribonucleoprotein U5 shows a surprisingly complex protein composition.

1989 • 148 citations

Immunoaffinity purification of a [U4/U6.U5] tri-snRNP from human cells.

1991 • 148 citations

Association of U6 snRNA with the 5'-splice site region of pre-mRNA in the spliceosome.

1992 • 143 citations

RNA metabolism: strategies for regulation in the heat shock response

1990 • 142 citations

Differential Binding of Heterogeneous Nuclear Ribonucleoproteins to mRNA Precursors prior to Spliceosome Assembly In Vitro

1992 • 142 citations

Two members of a conserved family of nuclear phosphoproteins are involved in pre-mRNA splicing.

1992 • 131 citations

Purification and visualization of native spliceosomes

1988 • 121 citations

The DNA Enzymology of Protein Machines

1984 • 115 citations

Protein composition of mammalian spliceosomes assembled in vitro.

1990 • 112 citations

Three protein factors (SF1, SF3 and U2AF) function in pre-splicing complex formation in addition to snRNPs.

1991 • 104 citations

Unscrambling the puzzle of biological machines: The importance of the details

1992 • 103 citations

Conservation between yeast and man of a protein associated with U5 small nuclear ribonucleoprotein

1989 • 101 citations

Identification of a functional mammalian spliceosome containing unspliced pre-mRNA.

1988 • 75 citations

The mammalian analogue of the yeast PRP8 splicing protein is present in the U4/5/6 small nuclear ribonucleoprotein particle and the spliceosome.

1989 • 74 citations

Characterisation of human and murine snRNP proteins by two-dimensional gel electrophoresis and phosphopeptide analysis of U1-specific 70K protein variants

1990 • 63 citations

A mammalian protein of 220 kDa binds pre-mRNAs in the spliceosome: a potential homologue of the yeast PRP8 protein.

1990 • 55 citations

Cytoplasmic Assembly and Nuclear Accumulation of Mature Small Nuclear Ribonucleoprotein Particles

1989 • 52 citations

Leucine periodicity of U2 small nuclear ribonucleoprotein particle (snRNP) A' protein is implicated in snRNP assembly via protein-protein interactions.

1991 • 46 citations

A novel splicing factor is an integral component of 200S large nuclear ribonucleoprotein (InRNP) particles.

1991 • 41 citations

Splicing factor SF4 is dispensable for the assembly of a functional splicing complex and participates in the subsequent steps of the splicing reaction.

1990 • 29 citations