Domains of yeast U4 spliceosomal RNA required for PRP4 protein binding, snRNP-snRNP interactions, and pre-mRNA splicing in vivo.

Methods in yeast genetics

1979 • 7,824 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

1987 • 5,413 citations

Improved free-energy parameters for predictions of RNA duplex stability.

1986 • 1,472 citations

[10] 5-Fluoroorotic acid as a selective agent in yeast molecular genetics

1987 • 1,375 citations

Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus

1979 • 1,178 citations

Splicing of Messenger RNA Precursors

1987 • 919 citations

The role of small nuclear ribonucleoprotein particles in pre-mRNA splicing

1987 • 594 citations

Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes

1987 • 530 citations

SPLICEOSOMAL snRNAs

1988 • 463 citations

Spliceosomal RNA U6 is remarkably conserved from yeast to mammals

1988 • 428 citations

Spliceosome assembly in yeast.

1987 • 402 citations

The structure of mammalian small nuclear ribonucleoproteins. Identification of multiple protein components reactive with anti-(U1)ribonucleoprotein and anti-Sm autoantibodies.

1984 • 371 citations

Structure and Function of Major and Minor Small Nuclear Ribonucleoprotein Particles

1988 • 363 citations

Yeast mRNA splicing in vitro.

1985 • 337 citations

Nuclear segregation of U2 snRNA requires binding of specific snRNP proteins

1985 • 307 citations

Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes

1986 • 283 citations

An ordered pathway of snRNP binding during mammalian pre-mRNA splicing complex assembly.

1987 • 272 citations

U4 and U6 RNAs coexist in a single small nuclear ribonucleoprotein particle

1984 • 238 citations

U2 RNA shares a structural domain with U1, U4, and U5 RNAs.

1982 • 236 citations

Pre-mRNA splicing in vitro requires intact U4/U6 small nuclear ribonucleoprotein

1986 • 222 citations

A family of versatile centromeric vectors designed for use in the sectoring-shuffle mutagenesis assay in Saccharomyces cerevisiae

1988 • 221 citations

Evidence for the existence of snRNAs U4 and U6 in a single ribonucleoprotein complex and for their association by intermolecular base pairing.

1984 • 213 citations

Small Nuclear RNAs: RNA Sequences, Structure, and Modifications

1988 • 202 citations

U1, U2, and U4/U6 small nuclear ribonucleoproteins are required for in vitro splicing but not polyadenylation

1986 • 192 citations

Spliceosome assembly involves the binding and release of U4 small nuclear ribonucleoprotein.

1988 • 188 citations

Identification of a yeast snRNP protein and detection of snRNP-snRNP interactions

1987 • 180 citations

Fungal small nuclear ribonucleoproteins share properties with plant and vertebrate U-snRNPs.

1987 • 171 citations

Purification of the individual snRNPs U1, U2, U5 and U4/U6 from HeLa cells and characterization of their protein constituents.

1986 • 170 citations

Antisense probing of the human U4U6 snRNP with biotinylated 2′-OMe RNA oligonucleotides

1989 • 169 citations

An essential yeast snRNA with a U5-like domain is required for splicing in vivo

1987 • 168 citations

20S small nuclear ribonucleoprotein U5 shows a surprisingly complex protein composition.

1989 • 148 citations

In vitro assembly of U1 snRNPs.

1987 • 145 citations

Localization of a base-paired interaction between small nuclear RNAs U4 and U6 in intact U4/U6 ribonucleoprotein particles by psoralen cross-linking

1985 • 137 citations

In vitro assembly of yeast U6 snRNP: a functional assay.

1989 • 134 citations

Yeast contains small nuclear RNAs encoded by single copy genes

1983 • 133 citations

PRP4: a protein of the yeast U4/U6 small nuclear ribonucleoprotein particle.

1989 • 131 citations

U5 small nuclear ribonucleoprotein: RNA structure analysis and ATP-dependent interaction with U4/U6.

1989 • 128 citations

Isolation and sequence of four small nuclear U RNA genes of Trypanosoma brucei subsp. brucei: identification of the U2, U4, and U6 RNA analogs.

1989 • 122 citations

An essential snRNA from S. cerevisiae has properties predicted for U4, including interaction with a U6-like snRNA

1987 • 104 citations

PRP4 (RNA4) from Saccharomyces cerevisiae: Its Gene Product Is Associated with the U4/U6 Small Nuclear Ribonucleoprotein Particle

1989 • 98 citations

Splicing a spliceosomal RNA

1989 • 95 citations

A Subset of Yeast snRNA's Contains Functional Binding Sites for the Highly Conserved Sm Antigen

1987 • 85 citations

Association of U2, U4, U5, and U6 small nuclear ribonucleoproteins in a spliceosome-type complex in absence of precursor RNA.

1988 • 71 citations

snRNP Proteins

1988 • 64 citations

RNA-protein organization of U1, U5 and U4-U6 small nuclear ribonucleoproteins in HeLa cells

1986 • 62 citations

Evidence for Related Functions of the RNA Genes of Saccharomyces cerevisiae

1987 • 52 citations

The PRP4 (RNA4) protein of Saccharomyces cerevisiae is associated with the 5' portion of the U4 small nuclear RNA.

1990 • 51 citations

Discrete domains of human U6 snRNA required for the assembly of U4/U6 snRNP and splicing complexes.

1990 • 49 citations

Modulation of yeast 5 S rRNA synthesis in vitro by ribosomal protein YL3. A possible regulatory loop.

1987 • 47 citations

In vitro reconstitution of snRNPs: a reconstituted U4/U6 snRNP participates in splicing complex formation.

1989 • 40 citations