Autophagy and other vacuolar protein degradation mechanisms

Functions of Lysosomes

1966 • 2,942 citations

Rapid redistribution of Golgi proteins into the ER in cells treated with brefeldin A: Evidence for membrane cycling from Golgi to ER

1989 • 1,685 citations

3-Methyladenine: Specific inhibitor of autophagic/lysosomal protein degradation in isolated rat hepatocytes

1982 • 1,313 citations

LYSOSOME FUNCTION IN THE REGULATION OF THE SECRETORY PROCESS IN CELLS OF THE ANTERIOR PITUITARY GLAND

1966 • 951 citations

A Role for a 70-Kilodalton Heat Shock Protein in Lysosomal Degradation of Intracellular Proteins

1989 • 932 citations

Effects of the tumour promoter okadaic acid on intracellular protein phosphorylation and metabolism

1989 • 848 citations

The mannose 6-phosphate receptor and the biogenesis of lysosomes

1988 • 846 citations

Mitochondrial protein import

1989 • 820 citations

Studies on the mechanisms of autophagy: formation of the autophagic vacuole.

1990 • 789 citations

Peptide sequences that target cytosolic proteins for lysosomal proteolysis

1990 • 674 citations

Protein degradation in the endoplasmic reticulum

1990 • 659 citations

MHC class II-associated invariant chain contains a sorting signal for endosomal compartments

1990 • 565 citations

Intracellular transport of class II MHC molecules directed by invariant chain

1990 • 548 citations

The retention signal for soluble proteins of the endoplasmic reticulum

1990 • 531 citations

Degradation from the endoplasmic reticulum: Disposing of newly synthesized proteins

1988 • 488 citations

Mannose 6-Phosphate Receptors and Lysosomal Enzyme Targeting

1989 • 473 citations

Inhibition of the Lysosomal Pathway of Protein Degradation in Isolated Rat Hepatocytes by Ammonia, Methylamine, Chloroquine and Leupeptin

1979 • 466 citations

Lysosomes : their role in protein breakdown

1987 • 435 citations

Intraorganellar calcium and pH control proinsulin cleavage in the pancreatic β cell via two distinct site-specific endopeptidases

1988 • 403 citations

Co-localization of molecules involved in antigen processing and presentation in an early endocytic compartment

1990 • 377 citations

Studies on the mechanisms of autophagy: maturation of the autophagic vacuole.

1990 • 352 citations

Vesicles and cisternae in the trans golgi apparatus of human fibroblasts are acidic compartments

1985 • 350 citations

Inhibition by Insulin of Valine Turnover in Liver

1970 • 338 citations

[59] Inhibitors of lysosomal function

1983 • 331 citations

Induction of autophagy by amino-acid deprivation in perfused rat liver

1977 • 323 citations

A novel pathway for secretory proteins?

1990 • 292 citations

Cell-free protein sorting to the regulated and constitutive secretory pathways

1990 • 289 citations

Proteolytic processing of the 600 kd low density lipoprotein receptor-related protein (LRP) occurs in a trans-Golgi compartment.

1990 • 279 citations

Prelysosomal convergence of autophagic and endocytic pathways

1988 • 272 citations

Cathepsin D is membrane-associated in macrophage endosomes.

1988 • 259 citations

A MORPHOMETRIC STUDY OF THE REMOVAL OF PHENOBARBITAL-INDUCED MEMBRANES FROM HEPATOCYTES AFTER CESSATION OF TREATMENT

1973 • 250 citations

In vitro degradation of extracellular matrix with Mr 52,000 cathepsin D secreted by breast cancer cells.

1988 • 242 citations

Selective control of the degradation of normal and aberrant proteins in Reuber H35 hepatoma cells

1976 • 234 citations

Glucagon-induced autophagy and proteolysis in rat liver: mediation by selective deprivation of intracellular amino acids.

1979 • 231 citations

A possible role for Na+,K+-ATPase in regulating ATP-dependent endosome acidification.

1989 • 229 citations

Regulation of endocytic pH by the Na+,K+-ATPase in living cells.

1989 • 228 citations

Macrophage endosomes contain proteases which degrade endocytosed protein ligands.

1985 • 223 citations

Quantitative relationship between autophagy and proteolysis during graded amino acid deprivation in perfused rat liver.

1981 • 223 citations

Nonselective autophagy of cytosolic enzymes by isolated rat hepatocytes.

1990 • 218 citations

Chloroquine diverts ACTH from a regulated to a constitutive secretory pathway in AtT-20 cells

1983 • 198 citations

In exocrine pancreas, the basolateral endocytic pathway converges with the autophagic pathway immediately after the early endosome.

1990 • 196 citations

Protein Degradation in Cultured Cells

1973 • 195 citations

Lysosomal acid phosphatase is transported to lysosomes via the cell surface.

1989 • 189 citations

Dissection of Semliki Forest virus glycoprotein delivery from the trans-Golgi network to the cell surface in permeabilized BHK cells.

1988 • 178 citations

Amino acid inhibition of the autophagic/lysosomal pathway of protein degradation in isolated rat hepatocytes

1980 • 172 citations

The identification of the major excreted protein (MEP) from a transformed mouse fibroblast cell line as a catalytically active precursor form of cathepsin L

1987 • 172 citations

Ammonia inhibition of protein degradation in isolated rat hepatocytes

1976 • 172 citations

Quantitative correlation between proteolysis and macro- and microautophagy in mouse hepatocytes during starvation and refeeding.

1983 • 171 citations

Amino acid control of autophagic sequestration and protein degradation in isolated rat hepatocytes.

1984 • 150 citations

Regulation of microautophagy and basal protein turnover in rat liver. Effects of short-term starvation.

1988 • 149 citations

Lysosomes

1989 • 147 citations

Intracellular degradation of unassembled asialoglycoprotein receptor subunits: a pre-Golgi, nonlysosomal endoproteolytic cleavage.

1989 • 146 citations

Trypanosome ornithine decarboxylase is stable because it lacks sequences found in the carboxyl terminus of the mouse enzyme which target the latter for intracellular degradation.

1990 • 145 citations

Selective degradation of T cell antigen receptor chains retained in a pre-Golgi compartment.

1988 • 139 citations

3‐Methyladenine, an inhibitor of autophagy, has multiple effects on metabolism

1988 • 131 citations

Cleavage of Parathyroid Hormone in Macrophage Endosomes Illustrates a Novel Pathway for Intracellular Processing of Proteins

1989 • 131 citations

The condensing vacuole of exocrine cells is more acidic than the mature secretory vesicle

1987 • 130 citations

Sequestration of cytoplasmic enzymes in an autophagic vacuole-lysosomal system induced by injection of leupeptin.

1983 • 128 citations

Intracellular protein catabolism

1977 • 118 citations

Uptake and degradation of proteins by isolated rat liver lysosomes. Suggestion of a microautophagic pathway of proteolysis.

1982 • 118 citations

Ultrastructural studies on prostatic involution in the rat. Mechanism of autophagy in epithelial cells, with special reference to the rough-surfaced endoplasmic reticulum

1971 • 115 citations

Differential effects of proteinase inhibitors and amines on the lysosomal and non-lysosomal pathways of protein degradation in isolated rat hepatocytes

1980 • 114 citations

Mannose 6-phosphate receptor dependent secretion of lysosomal enzymes.

1990 • 114 citations

Intracellular degradation of the transport-impaired human PiZ alpha 1-antitrypsin variant. Biochemical mapping of the degradative event among compartments of the secretory pathway.

1990 • 111 citations

THE ORIGIN AND FATE OF MICROBODIES IN THE FAT BODY OF AN INSECT

1971 • 111 citations

Effect of microtubular or translational inhibitors on general cell protein degradation. Evidence for a dual catabolic pathway

1977 • 110 citations

Energy Dependence of Different Steps in the Autophagic-Lysosomal Pathway

1989 • 106 citations

Selective degradation of insulin within rat liver endosomes.

1990 • 106 citations

Inhibition of autophagic vacuole formation and protein degradation by amino acids in isolated hepatocytes

1981 • 102 citations

Reduced autophagic activity, improved protein balance and enhanced in vitro survival of hepatocytes isolated from carcinogen-treated rats

1985 • 99 citations

Amino acid and hormonal control of macromolecular turnover in perfused rat liver. Evidence for selective autophagy.

1987 • 99 citations

Energy dependence of autophagic protein degradation in isolated rat hepatocytes

1987 • 99 citations

Protein degradation in isolated rat hepatocytes is inhibited by ammonia

1975 • 99 citations

Regulation of intracellular protein degradation in IMR‐90 human diploid fibroblasts

1983 • 99 citations

B lymphocyte differentiation and the control of IgM μ chain expression

1981 • 98 citations

Characterization of pro-opiocortin-converting activity in purified secretory granules from rat pituitary neurointermediate lobe.

1982 • 98 citations

Localization of transferrin receptors and insulin-like growth factor II receptors in vesicles from 3T3-L1 adipocytes that contain intracellular glucose transporters.

1989 • 96 citations

Degradation of ornithine decarboxylase in mammalian cells is ATP dependent but ubiquitin independent

1989 • 93 citations

Inhibition of autophagic-lysosomal delivery and autophagic lactolysis by asparagine.

1991 • 91 citations

Chapter 6 Intracellular proteolysis

1987 • 89 citations

Autophagic sequestration of 614C9sucrose, introduced into rat hepatocytes by reversible electro-permeabilization*1

1982 • 86 citations

Internalization of cytoplasmic protein by hepatic lysosomes in basal and deprivation-induced proteolytic states.

1981 • 84 citations

Regulation of catabolism of microinjected ribonuclease A requires the amino-terminal 20 amino acids.

1983 • 81 citations

Mechanism for selective secretion of a lysosomal protease by transformed mouse fibroblasts

1989 • 79 citations

Increased degradation in rat liver induced by vinblastine. II. Morphologic characterization.

1980 • 76 citations

Protein transport across the ER membrane

1990 • 75 citations

Inhibition of protein breakdown in cultured cells is a consistent response to growth factors

1980 • 75 citations

Mechanisms of membrane protein turnover

1990 • 73 citations

Intracellular protein degradation in growing, in density‐inhibited, and in serum‐restricted fibroblast cultures

1977 • 73 citations

The effect of protein degradation on cellular growth characteristics

1978 • 72 citations

Inhibition of mouse macrophage degradation of acetyl-low density lipoprotein by interferon-gamma.

1990 • 72 citations

Intracellular Transport, Sorting, and Turnover of Acetylcholinesterase

1989 • 70 citations

Disparate effects of monensin and colchicine on intracellular processing of secretory proteins in cultured rat hepatocytes

1983 • 70 citations

Phosphorylation of Native 97-kDa 3-Hydroxy-3-methylglutaryl-coenzyme A Reductase from Rat Liver

1989 • 70 citations

Heparin decreases the degradation rate of lipoprotein lipase in adipocytes.

1987 • 69 citations

Calcium ion-dependent vesicle fusion in the conversion of proalbumin to albumin

1978 • 68 citations

Lysosomal degradation of ribonuclease A and ribonuclease S-protein microinjected into the cytosol of human fibroblasts.

1985 • 65 citations

Characterization of an endopeptidase involved in pre-protein processing.

1979 • 63 citations

The dissociation and degradation of internalized insulin occur in the endosomes of rat hepatoma cells.

1990 • 63 citations

Inhibition of hepatocytic protein degradation by methylaminopurines and inhibitors of protein synthesis

1981 • 62 citations

Modulation of the transport of a lysosomal enzyme by PDGF.

1990 • 59 citations

Differences in targeting and secretion of cathepsins B and L by BALB/3T3 fibroblasts and Moloney murine sarcoma virus-transformed BALB/3T3 fibroblasts.

1990 • 56 citations

Metalloendoprotease Inhibitors Which Block the Differentiation of L6 Myoblasts Inhibit Insulin Degradation by the Endogenous Insulin-degrading Enzyme

1989 • 54 citations

Inhibition of catabolism of radiolabeled antibodies by tumor cells using lysosomotropic amines and carboxylic ionophores.

1990 • 54 citations

Protein synthesis and protein degradation through the cell cycle of human NHIK 3025 cells in vitro

1979 • 54 citations

Effects of weakly basic amines on proteolytic processing and terminal glycosylation of secretory proteins in cultured rat hepatocytes

1986 • 53 citations

Effects of insulin and anchorage on hepatocytic protein metabolism and amino acid transport

1981 • 49 citations

Secretion of Intact Proteins and Peptide Fragments by Lysosomal Pathways of Protein Degradation

1989 • 48 citations

Use of [3H]raffinose as a specific probe of autophagic sequestration

1986 • 48 citations

Use of a hydrolysable probe, [14C]lactose, to distinguish between pre-lysosomal and lysosomal steps in the autophagic pathway

1986 • 48 citations

Autophagy of metabolically inert substances injected into fibroblasts in culture

1981 • 47 citations

Covalent linkage of ribonuclease S-peptide to microinjected proteins causes their intracellular degradation to be enhanced during serum withdrawal.

1986 • 45 citations

Solubilization and characterization of yeast signal peptidase

1989 • 45 citations

In vitro uptake of particles by lysosomes

1980 • 44 citations

The role of protein accumulation in the cell cycle control of human NHIK 3025 cells

1981 • 43 citations

Kinetics of intracellular degradation of newly synthesized collagen

1986 • 43 citations

Ammonium Chloride Alters Secretory Protein Sorting within the Maturing Exocrine Storage Compartment

1989 • 41 citations

Inhibition of lysosomal protein degradation inhibits the basal degradation of 3-hydroxy-3-methylglutaryl coenzyme A reductase.

1987 • 40 citations

Extensive destruction of newly synthesized casein in mammary explants in organ culture

1982 • 40 citations

Post-translational arginylation of ornithine decarboxylase from rat hepatocytes

1990 • 38 citations

Reconstitution of Endosomal Proteolysis in a Cell-free System

1989 • 38 citations

RNA degradation in perfused rat liver as determined from the release of [14C]cytidine.

1987 • 37 citations

Ammonia inhibits protein secretion in isolated rat hepatocytes

1977 • 37 citations

Weakly basic amines inhibit the proteolytic conversion of proalbumin to serum albumin in cultured rat hepatocytes

1985 • 36 citations

Protein synthesis and degradation in growth regulation in rat embryo fibroblasts: Role of fast‐turnover and slow‐turnover protein

1980 • 36 citations

Peptide signals for protein degradation within lysosomes.

1989 • 35 citations

Rates of RNA degradation in isolated rat hepatocytes

1990 • 34 citations

Both endocytic and endogenous protein degradation in fibroblasts is stimulated by serum/amino acid deprivation and inhibited by 3-methyladenine

1990 • 34 citations

Regulation of hepatic HMG-CoA reductase in vivo by reversible phosphorylation

1987 • 33 citations

Ammonium chloride causes reversible inhibition of low density lipoprotein receptor recycling and accelerates receptor degradation.

1990 • 33 citations

Conversion of dense lysosomes into light lysosomes during hepatocytic autophagy

1985 • 32 citations

Control of Protein Degradation and Growth Phase in Normal and Neoplastic Cells

1984 • 32 citations

Effect of serum step‐down on protein metabolism and proliferation kinetics of NHIK 3025 cells

1981 • 32 citations

Differences in the rates of protein degradation in untransformed and transformed cell lines

1978 • 31 citations

6-Substituted purines: A novel class of inhibitors of endogenous protein degradation in isolated rat hepatocytes

1982 • 30 citations

Temperature dependence of protein degradation, autophagic sequestration and mitochondrial sugar uptake in rat hepatocytes

1987 • 30 citations

Use of digitonin extraction to distinguish between autophagic-lysosomal sequestration and mitochondrial uptake of [14C]sucrose in hepatocytes

1985 • 30 citations

Activation of neutrophil calpain following its translocation to the plasma membrane induced by phorbol ester or fMet-Leu-Phe

1989 • 30 citations

Kinetics of hydrolysis of endocytosed substrates by mammalian cultured cells: Early introduction of lysosomal enzymes into the endocytic pathway

1990 • 30 citations

Regulation of intracellular casein degradation by secreted milk proteins

1989 • 29 citations

Experimental characterization of the autophagic-lysosomal pathway in isolated rat hepatocytes.

1989 • 29 citations

Intracellular protein degradation in serum-deprived human fibroblasts

1986 • 29 citations

Pinocytosis and intracellular degradation of exogenous protein: modulation by amino acids.

1983 • 28 citations

Inhibitors and pathways of hepatocytic protein degradation.

1981 • 28 citations

Autophagic sequestration of [14C]sucrose introduced into isolated rat hepatocytes by electrical and non-electrical methods

1985 • 27 citations

Degradation of structurally characterized proteins injected into HeLa cells. Basic measurements.

1988 • 26 citations

Ammonium chloride inhibits basal degradation of newly synthesized collagen in human fetal lung fibroblasts

1990 • 25 citations

Transferrin receptors and cation-independent mannose-6-phosphate receptors deliver their ligands to two distinct subpopulations of multivesicular endosomes.

1989 • 25 citations

Intermediate cells of the pancreas: III. selective autophagy and destruction of β-granules in intermediate cells of the rat pancreas induced by alloxan and streptozotocin

1973 • 24 citations

Rapid turnover of adenovirus E1A is determined through a co-translational mechanism that requires an aminoterminal domain.

1988 • 24 citations

Degradation of structurally characterized proteins injected into HeLa cells. Effects of intracellular location and the involvement of lysosomes.

1988 • 24 citations

Swainsonine inhibits glycoprotein degradation by isolated rat liver lysosomes.

1984 • 23 citations

Effects of puromycin and hydroxynorvaline on net production and intracellular degradation of collagen in human fetal lung fibroblasts

1989 • 23 citations

Degradation of insulin in isolated liver endosomes is functionally linked to ATP‐dependent endosomal acidification

1990 • 22 citations

Hepatocytic autophagy.

1991 • 21 citations

The relationship between beta-adrenoceptor regulation and beta-adrenergic responsiveness in hepatocytes. Studies on acquisition, desensitization and resensitization of isoproterenol-sensitive adenylate cyclase in primary culture

1987 • 20 citations

Intracellular degradation of asialoglycoproteins in hepatocytes starts in a subgroup of lysosomes

1985 • 19 citations

Resolution of multiple endosomal compartments associated with the internalization of epidermal growth factor and transferrin

1987 • 19 citations

Metalloendoprotease inhibitors block protein synthesis, intracellular transport, and endocytosis in hepatoma cells.

1988 • 18 citations

Purification and characterization of a novel calcium-dependent serine proteinase secreted from malignant hamster embryo fibroblast Nil2C2

1989 • 17 citations

Participation of lysosomes in basal proteolysis in perfused rat liver

1985 • 17 citations

[Lysosomes and autophagy (author's transl)].

1976 • 17 citations

Proteolytic Processing in Endosomal Vesicles

1989 • 17 citations

A unique membrane-bound, calcium-dependent endopeptidase with specificity toward paired basic residues in rat liver Golgi fractions

1989 • 16 citations

Differential effects of leupeptin, monensin and colchicine on ligand degradation mediated by the two asialoglycoprotein receptor pathways in isolated rat hepatocytes

1989 • 16 citations

Methods in laboratory investigation. Application of test substances to the surface of rat liver in situ: opposite effects of insulin and isoproterenol on cellular autophagy.

1984 • 15 citations

Proteolytic processing in a non-lysosomal compartment is required for transcytosis of protein-polylysine conjugates in cultured Madin-Darby canine kidney cells

1990 • 15 citations

Convergence of autophagic and endocytic pathways at the level of the lysosome

1987 • 14 citations

Amino acid control of protein degradation in normal and leukemic human lymphocytes

1984 • 14 citations

Rapid turnover of low‐density lipoprotein receptor by a non‐lysosomal pathway in mouse macrophage J774 cells and inhibitory effect of brefeldin A

1990 • 14 citations

Estradiol increases the secretion by MCF7 cells of several lysosomal pro-enzymes

1990 • 14 citations

Transport of acid phosphatase to lysosomes does not involve passage through the cell surface

1990 • 13 citations

Role of cyclic nucleotides in the control of hepatic autophagy.

1991 • 13 citations

Use of electrical methods in the study of hepatocytic autophagy.

1986 • 13 citations

Lysosomal acid phosphatase is transported via endosomes to lysosomes

1990 • 12 citations

IN VIVO DETERMINATION OF RATES OF PROTEIN DEGRADATION IN LIVERS OF MEAL-FED RATS: IMPLICATIONS OF AMINO ACID COMPARTMENTATION

1978 • 12 citations

Radiolabelled sugars as probes of hepatocytic autophagy.

1986 • 11 citations

Effects of acidotropic compounds on the secretory pathway: Inhibition of secretion and processing of the third and fourth components of complement

1986 • 10 citations

The invariant dating service

1990 • 10 citations

[Morphological and functional aspects of cellular autophagy].

1972 • 10 citations

Secretion-coupled protein degradation: Studies on mammary casein

1986 • 9 citations

Stimulation of lysosomal enzyme secretion by growth factors

1990 • 9 citations

Effect of amino acid metabolites on lysosomal protein degradation. A regulatory role for kynurenine?

1984 • 8 citations

Degradation of horseradish peroxidase after microinjection into mammalian cells

1988 • 8 citations

Paradoxical stimulation by amino acids of the degradation of [35S]methionine-labelled, short-lived protein in isolated rat hepatocytes

1983 • 5 citations

Sequestration and hydrolysis of electroinjected [14C]lactose as a means of investigating autophagosome-lysosome fusion in isolated rat hepatocytes.

1985 • 5 citations

Pathways of intracellular sequestration and protein degradation in isolated rat hepatocytes.

1985 • 4 citations

Effects of adrenergic agonists and antagonists on autophagic activity in isolated rat liver cells.

1991 • 4 citations