A compensatory base change in human U2 snRNA can suppress a branch site mutation.

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1984 • 6,459 citations

A compensatory base change in U1 snRNA suppresses a 5′ splice site mutation

1986 • 755 citations

Normal and mutant human β-globin pre-mRNAs are faithfully and efficiently spliced in vitro

1984 • 679 citations

The U1 small nuclear RNA-protein complex selectively binds a 5′ splice site in vitro

1983 • 593 citations

U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing

1985 • 568 citations

A factor, U2AF, is required for U2 snRNP binding and splicing complex assembly

1988 • 535 citations

Recognition of the TACTAAC box during mRNA splicing in yeast involves base pairing to the U2-like snRNA

1987 • 508 citations

Multiple factors including the small nuclear ribonucleoproteins U1 and U2 are necessary for Pre-mRNA splicing in vitro

1985 • 442 citations

Intron sequences involved in lariat formation during pre-mRNA splicing

1985 • 402 citations

A U1 snRNA:pre-mRNA base pairing interaction is required early in yeast spliceosome assembly but does not uniquely define the 5′ cleavage site.

1988 • 364 citations

Intron splicing: a conserved internal signal in introns of animal pre-mRNAs.

1984 • 342 citations

Point mutations identify the conserved, intron-contained TACTAAC box as an essential splicing signal sequence in yeast

1984 • 339 citations

Mammalian pre-mRNA branch site selection by U2 snRNP involves base pairing.

1989 • 314 citations

The 3′ Splice Site of Pre-Messenger RNA Is Recognized by a Small Nuclear Ribonucleoprotein

1985 • 310 citations

Functions of the Abundant U-snRNPs

1988 • 284 citations

A protein associated with small nuclear ribonucleoprotein particles recognizes the 3′ splice site of premessenger RNA

1986 • 283 citations

A protein that specifically recognizes the 3′ splice site of mammalian pre-mRNA introns is associated with a small nuclear ribonucleoprotein

1986 • 274 citations

An Early Hierarchic Role of U1 Small Nuclear Ribonucleoprotein in Spliceosome Assembly

1988 • 271 citations

RNA binding specificity of hnRNP proteins: a subset bind to the 3′ end of introns.

1988 • 251 citations

Mutually exclusive splicing of α-tropomyosin exons enforced by an unusual lariat branch point location: Implications for constitutive splicing

1989 • 238 citations

Cryptic branch point activation allows accurate in vitro splicing of human β-globin intron mutants

1985 • 236 citations

β+ Thalassemia: Aberrant splicing results from a single point mutation in an intron

1981 • 235 citations

The role of the mammalian branchpoint sequence in pre-mRNA splicing.

1988 • 215 citations

Gel electrophoretic isolation of splicing complexes containing U1 small nuclear ribonucleoprotein particles.

1988 • 183 citations

UACUAAC is the preferred branch site for mammalian mRNA splicing.

1989 • 180 citations

U2 RNA from yeast is unexpectedly large and contains homology to vertebrate U4, U5, and U6 small nuclear RNAs

1986 • 160 citations

Additional low-abundance human small nuclear ribonucleoproteins: U11, U12, etc.

1988 • 138 citations

Sequences required for 3′ end formation of human U2 small nuclear RNA

1985 • 122 citations

Presplicing complex formation requires two proteins and U2 snRNP.

1988 • 117 citations

Human U2 small nuclear RNA genes contain an upstream enhancer.

1986 • 112 citations

Effect of mutations at the lariat branch acceptor site on β-globin pre-mRNA splicing in vitro

1986 • 111 citations

The role of the polypyrimidine stretch at the SV40 early pre-mRNA 3′ splice site in alternative splicing.

1988 • 105 citations

Nonconsensus branch-site sequences in the in vitro splicing of transcripts of mutant rabbit beta-globin genes.

1985 • 96 citations

Two conserved domains of yeast U2 snRNA are separated by 945 nonessential nucleotides

1988 • 93 citations

Abnormally spliced messenger RNA in erythroid cells from patients with β+ thalassemia and monkey cells expressing a cloned β+-thalassemic gene

1982 • 91 citations

The natural 5' splice site of simian virus 40 large T antigen can be improved by increasing the base complementarity to U1 RNA.

1987 • 84 citations

U1 small nuclear ribonucleoproteins are required early during spliceosome assembly.

1987 • 80 citations

Alternative splicing of SV40 early pre-mRNA is determined by branch site selection.

1988 • 77 citations

Unusual branch point selection in processing of human growth hormone pre-mRNA.

1988 • 72 citations

Different Small Nuclear Ribonucleoprotein Particles Are Involved in Different Steps of Splicing Complex Formation

1987 • 68 citations

Internal sequences that distinguish yeast from metazoan U2 snRNA are unnecessary for pre-mRNA splicing

1988 • 66 citations

Rapid and efficient site-specific mutagenesis

1990 • 45 citations

Role of the branch site/3'-splice site region in adenovirus-2 E1A pre-mRNA alternative splicing: evidence for 5'- and 3'-splice site co-operation

1989 • 34 citations

Genetic analysis of the role of human U1 snRNA in mRNA splicing: I. Effect of mutations in the highly conserved stem-loop I of U1.

1989 • 27 citations

A single nucleotide difference at the 3′ end of an intron causes differential splicing of two histocompatibility genes.

1986 • 22 citations