Biochemical Mechanisms of Constitutive and Regulated Pre-mRNA Splicing

A catalogue of splice junction sequences

1982 • 3,861 citations

RNA splice junctions of different classes of eukaryotes: sequence statistics and functional implications in gene expression

1987 • 2,333 citations

The HIV-1 rev trans-activator acts through a structured target sequence to activate nuclear export of unspliced viral mRNA

1989 • 1,280 citations

Splicing of Messenger RNA Precursors

1984 • 874 citations

Excision of an intact intron as a novel lariat structure during pre-mRNA splicing in vitro

1984 • 761 citations

A compensatory base change in U1 snRNA suppresses a 5′ splice site mutation

1986 • 755 citations

Exon definition may facilitate splice site selection in RNAs with multiple exons.

1990 • 726 citations

Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.

1981 • 725 citations

Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus

1990 • 723 citations

Normal and mutant human β-globin pre-mRNAs are faithfully and efficiently spliced in vitro

1984 • 679 citations

PRE-mRNA SPLICING

1986 • 673 citations

The U1 small nuclear RNA-protein complex selectively binds a 5′ splice site in vitro

1983 • 593 citations

Functional Organization Of The Nuclear Envelope

1988 • 593 citations

A survey on intron and exon lengths

1988 • 569 citations

A mechanism for RNA splicing.

1980 • 553 citations

A factor, U2AF, is required for U2 snRNP binding and splicing complex assembly

1988 • 535 citations

Stepwise assembly of a pre-mRNA splicing complex requires U-snRNPs and specific intron sequences

1985 • 524 citations

Regulation by HIV Rev depends upon recognition of splice sites

1989 • 517 citations

Recognition of the TACTAAC box during mRNA splicing in yeast involves base pairing to the U2-like snRNA

1987 • 508 citations

A protein factor, ASF, controls cell-specific alternative splicing of SV40 early pre-mRNA in vitro

1990 • 500 citations

A role for exon sequences and splice-site proximity in splice-site selection

1986 • 494 citations

Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs

1986 • 490 citations

Lariat RNA's as Intermediates and Products in the Splicing of Messenger RNA precursors

1984 • 474 citations

Sex-lethal, a Drosophila sex determination switch gene, exhibits sex-specific RNA splicing and sequence similarity to RNA binding proteins

1988 • 461 citations

A multicomponent complex is involved in the splicing of messenger RNA precursors

1985 • 451 citations

Multiple factors including the small nuclear ribonucleoproteins U1 and U2 are necessary for Pre-mRNA splicing in vitro

1985 • 442 citations

Spliceosomal RNA U6 is remarkably conserved from yeast to mammals

1988 • 428 citations

Some cis- and trans-acting mutants for splicing target pre-mRNA to the cytoplasm

1989 • 417 citations

Monomethylated cap structures facilitate RNA export from the nucleus

1990 • 411 citations

The 5′ terminus of the RNA moiety of U1 small nuclear ribonucleoprotein particles is required for the splicing of messenger RNA precursors

1984 • 404 citations

Spliceosome assembly in yeast.

1987 • 402 citations

Heterogeneous Nuclear Ribonucleoproteins: Role in RNA Splicing

1986 • 399 citations

Sequence requirements for splicing of higher eukaryotic nuclear pre-mRNA

1986 • 389 citations

A U1 snRNA:pre-mRNA base pairing interaction is required early in yeast spliceosome assembly but does not uniquely define the 5′ cleavage site.

1988 • 364 citations

Structure and Function of Major and Minor Small Nuclear Ribonucleoprotein Particles

1988 • 363 citations

Purification and characterization of pre-mRNA splicing factor SF2 from HeLa cells.

1990 • 359 citations

Requirement of the RNA helicase-like protein PRP22 for release of messenger RNA from spliceosomes

1991 • 354 citations

Yeast mRNA splicing in vitro.

1985 • 337 citations

PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome

1991 • 329 citations

Mammalian pre-mRNA branch site selection by U2 snRNP involves base pairing.

1989 • 314 citations

The organization of 3' splice-site sequences in mammalian introns.

1989 • 310 citations

5' splice site selection in yeast: genetic alterations in base-pairing with U1 reveal additional requirements.

1988 • 307 citations

Sex-specific alternative splicing of RNA from the transformer gene results from sequence-dependent splice site blockage

1989 • 288 citations

A protein associated with small nuclear ribonucleoprotein particles recognizes the 3′ splice site of premessenger RNA

1986 • 283 citations

Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes

1986 • 283 citations

A protein that specifically recognizes the 3′ splice site of mammalian pre-mRNA introns is associated with a small nuclear ribonucleoprotein

1986 • 274 citations

Alternative splicing caused by RNA secondary structure

1985 • 274 citations

An ordered pathway of snRNP binding during mammalian pre-mRNA splicing complex assembly.

1987 • 272 citations

The sex-determining gene tra-2 of Drosophila encodes a putative RNA binding protein

1988 • 266 citations

Mechanisms of Alternative Pre-mRNA Splicing

1991 • 257 citations

Biochemical characterization of U2 snRNP auxiliary factor: an essential pre-mRNA splicing factor with a novel intranuclear distribution.

1991 • 255 citations

Signals for the selection of a splice site in pre-mRNA

1987 • 247 citations

Mammalian nuclei contain foci which are highly enriched in components of the pre-mRNA splicing machinery.

1991 • 243 citations

Affinity Chromatography of Splicing Complexes: U2, U5, and U4 + U6 Small Nuclear Ribonucleoprotein Particles in the Spliceosome

1986 • 238 citations

Mutually exclusive splicing of α-tropomyosin exons enforced by an unusual lariat branch point location: Implications for constitutive splicing

1989 • 238 citations

Mature mRNA 3′ end formation stimulates RNA export from the nucleus.

1991 • 222 citations

The role of the mammalian branchpoint sequence in pre-mRNA splicing.

1988 • 215 citations

Mutations in conserved intron sequences affect multiple steps in the yeast splicing pathway, particularly assembly of the spliceosome.

1986 • 215 citations

Trans splicing involves a novel form of small nuclear ribonucleoprotein particles

1988 • 210 citations

Pre-mRNA Splicing in Yeast

1989 • 209 citations

A monoclonal antibody against the nuclear pore complex inhibits nucleocytoplasmic transport of protein and RNA in vivo.

1988 • 206 citations

Protein-mediated nuclear export of RNA: 5S rRNA containing small RNPs in xenopus oocytes

1990 • 205 citations

tRNA transport from the nucleus in a eukaryotic cell: carrier-mediated translocation process.

1983 • 202 citations

mRNA splicing efficiency in yeast and the contribution of nonconserved sequences

1985 • 194 citations

Mutant phenotypes support a trans-splicing mechanism for the expression of the tripartite psaA gene in the C. reinhardtii chloroplast

1988 • 190 citations

Spliceosome assembly involves the binding and release of U4 small nuclear ribonucleoprotein.

1988 • 188 citations

Gamma-monomethyl phosphate: a cap structure in spliceosomal U6 small nuclear RNA.

1989 • 185 citations

Identification of a yeast snRNP protein and detection of snRNP-snRNP interactions

1987 • 180 citations

Trans splicing of mrna precursors in vitro

1985 • 180 citations

Multiple roles for U6 snRNA in the splicing pathway.

1990 • 179 citations

Molecular consequences of specific intron mutations on yeast mRNA splicing in vivo and in vitro

1985 • 172 citations

Fungal small nuclear ribonucleoproteins share properties with plant and vertebrate U-snRNPs.

1987 • 171 citations

Two Domains of Yeast U6 Small Nuclear RNA Required for Both Steps of Nuclear Precursor Messenger RNA Splicing

1990 • 171 citations

Evidence for base-pairing between mammalian U2 and U6 small nuclear ribonucleoprotein particles.

1990 • 171 citations

Antisense probing of the human U4U6 snRNP with biotinylated 2′-OMe RNA oligonucleotides

1989 • 169 citations

The Rat α-Tropomyosin Gene Generates a Minimum of Six Different mRNAs Coding for Striated, Smooth, and Nonmuscle Isoforms by Alternative Splicing

1988 • 167 citations

Splice site selection and ribonucleoprotein complex assembly during in vitro pre-mRNA splicing.

1988 • 163 citations

A binding consensus: RNA-protein interactions in splicing, snRNPs, and sex

1989 • 162 citations

Cap-dependent RNA splicing in a HeLa nuclear extract.

1985 • 156 citations

Mutations in a yeast intron demonstrate the importance of specific conserved nucleotides for the two stages of nuclear mRNA splicing

1986 • 150 citations

Regulated Splicing of the Drosophila P Transposable Element Third Intron in Vitro: Somatic Repression

1990 • 149 citations

Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with RNA-binding motifs.

1991 • 148 citations

The role of nucleotide sequences in splice site selection in eukaryotic pre-messenger RNA

1986 • 148 citations

A cold-sensitive mRNA splicing mutant is a member of the RNA helicase gene family.

1991 • 147 citations

Lethal and temperature-sensitive mutations and their suppressors identify an essential structural element in U2 small nuclear RNA.

1990 • 147 citations

Multiple interactions between the splicing substrate and small nuclear ribonucleoproteins in spliceosomes.

1987 • 143 citations

Targeted snRNP depletion reveals an additional role for mammalian U1 snRNP in spliceosome assembly

1990 • 143 citations

Intranuclear localization of snRNP antigens.

1986 • 140 citations

The C. elegans Trans-spliced leader RNA is bound to Sm and has a trimethylguanosine cap

1988 • 139 citations

The yeast RNA gene products are essential for mRNA splicing in vitro

1986 • 138 citations

Splicing of yeast nuclear pre-mRNA in vitro requires a functional 40S spliceosome and several extrinsic factors.

1987 • 137 citations

S. cerevisiae U1 RNA is large and has limited primary sequence homology to metazoan U1 snRNA

1987 • 131 citations

PRP4: a protein of the yeast U4/U6 small nuclear ribonucleoprotein particle.

1989 • 131 citations

A point mutation in the conserved hexanucleotide at a yeast 5′ splice junction uncouples recognition, cleavage, and ligation

1985 • 129 citations

Domains of U4 and U6 snRNAs required for snRNP assembly and splicing complementation in Xenopus oocytes.

1990 • 127 citations

The gene for the U6 small nuclear RNA in fission yeast has an intron

1989 • 126 citations

PRP5: a helicase-like protein required for mRNA splicing in yeast.

1990 • 123 citations

Evidence against a scanning model of RNA splicing.

1983 • 122 citations

Saccharomyces cerevisiae Has a U1-Like Small Nuclear RNA with Unexpected Properties

1987 • 122 citations

Factors influencing alternative splice site utilization in vivo.

1987 • 120 citations

tRNA nuclear transport: Defining the critical regions of human tRNAimet by point mutagenesis

1985 • 118 citations

Preferential excision of the 5' proximal intron from mRNA precursors with two introns as mediated by the cap structure.

1987 • 114 citations

Effect of mutations at the lariat branch acceptor site on β-globin pre-mRNA splicing in vitro

1986 • 111 citations

An essential snRNA from S. cerevisiae has properties predicted for U4, including interaction with a U6-like snRNA

1987 • 104 citations

Precision and orderliness in splicing

1987 • 104 citations

Exon mutations that affect the choice of splice sites used in processing the SV40 late transcripts

1985 • 103 citations

Functional analysis of mutant Xenopus U2 snRNAs

1989 • 103 citations

Conservation between yeast and man of a protein associated with U5 small nuclear ribonucleoprotein

1989 • 101 citations

PRP4 (RNA4) from Saccharomyces cerevisiae: Its Gene Product Is Associated with the U4/U6 Small Nuclear Ribonucleoprotein Particle

1989 • 98 citations

The yeast branchpoint sequence is not required for the formation of a stable U1 snRNA-pre-mRNA complex and is recognized in the absence of U2 snRNA.

1991 • 97 citations

In vitro splicing of mutually exclusive exons from the chicken beta-tropomyosin gene: role of the branch point location and very long pyrimidine stretch.

1990 • 97 citations

Splicing a spliceosomal RNA

1989 • 95 citations

A trans-acting suppressor restores splicing of a yeast intron with a branch point mutation.

1987 • 95 citations

Domains of yeast U4 spliceosomal RNA required for PRP4 protein binding, snRNP-snRNP interactions, and pre-mRNA splicing in vivo.

1990 • 95 citations

Two conserved domains of yeast U2 snRNA are separated by 945 nonessential nucleotides

1988 • 93 citations

Trans splicing of nematode pre-messenger RNA in vitro

1990 • 93 citations

RNA Splice Site Selection: Evidence for a 5′ → 3′ Scanning Model

1983 • 92 citations

Spliced leader RNA sequences can substitute for the essential 5′ end of U1 RNA during splicing in a mammalian in vitro system

1990 • 92 citations

In vitro reconstitution of functional yeast U2 snRNPs.

1989 • 91 citations

Branch point selection in alternative splicing of tropomyosin pre-mRNAs

1989 • 89 citations

Post-transcriptional processing of simian virus 40 late transcripts in injected frog oocytes

1983 • 88 citations

The U2 RNA analogue ofTrypanosoma brucei gambiense: implications for a splicing mechanism in trypanosomes

1986 • 86 citations

The molecular characterization of PRP6 and PRP9 yeast genes reveals a new cysteine/histidine motif common to several splicing factors.

1990 • 84 citations

Interactions of PRP2 protein with pre-mRNA splicing complexes inSaccharomyces cerevisiae

1990 • 83 citations

5′-terminal caps of snRNAs are reactive with antibodies specific for 2,2,7-trimethylguanosine in whole cells and nuclear matrices

1984 • 79 citations

Electron microscopy of small nuclear ribonucleoprotein (snRNP) particles U2 and U5: evidence for a common structure-determining principle in the major U snRNP family.

1990 • 78 citations

The v- and c-myc oncogene proteins colocalize in situ with small nuclear ribonucleoprotein particles.

1987 • 77 citations

Alternative splicing of SV40 early pre-mRNA is determined by branch site selection.

1988 • 77 citations

PRP18, a protein required for the second reaction in pre-mRNA splicing.

1990 • 73 citations

Cloning of the RNA8 gene of Saccharomyces cerevisiae, detection of the RNA8 protein, and demonstration that it is essential for nuclear pre-mRNA splicing.

1988 • 72 citations

Internal sequences that distinguish yeast from metazoan U2 snRNA are unnecessary for pre-mRNA splicing

1988 • 66 citations

C.elegansmRNAs acquire a spliced leader through atrans-splicing mechanism

1988 • 65 citations

Impaired nuclear transport of a human variant tRNAiMet

1982 • 64 citations

U2 snRNA sequences that bind U2-specific proteins are dispensable for the function of U2 snRNP in splicing.

1989 • 64 citations

Affinity purification of spliceosomes reveals that the precursor RNA processing protein PRP8, a protein in the U5 small nuclear ribonucleoprotein particle, is a component of yeast spliceosomes.

1990 • 59 citations

Multiple domains of U1 snRNA, including U1 specific protein binding sites, are required for splicing.

1990 • 59 citations

Inhibition of splicing but not cleavage at the 5' splice site by truncating human beta-globin pre-mRNA.

1986 • 58 citations

Separation of multiple components of HeLa cell nuclear extracts required for pre-messenger RNA splicing.

1987 • 56 citations

Saccharomyces cerevisiae U1 small nuclear RNA secondary structure contains both universal and yeast-specific domains.

1990 • 55 citations

A mammalian protein of 220 kDa binds pre-mRNAs in the spliceosome: a potential homologue of the yeast PRP8 protein.

1990 • 55 citations

Isolation and characterization of two fractions from HeLa cells required for mRNA splicing in vitro.

1985 • 51 citations

Human U2 snRNA can function in pre-mRNA splicing in yeast

1990 • 50 citations

The nematode spliced leader RNA participates in trans-splicing as an Sm snRNP.

1990 • 42 citations

A novel splicing factor is an integral component of 200S large nuclear ribonucleoprotein (InRNP) particles.

1991 • 41 citations

Universally conserved and yeast-specific U1 snRNA sequences are important but not essential for U1 snRNP function.

1990 • 41 citations

Conserved domains of human U4 snRNA required for snRNP and spliceosome assembly

1990 • 37 citations

Direct cross-linking of snRNP proteins F and 70K to snRNAs by ultra-violet radiationin situ

1988 • 28 citations

Structure of a pre-mRNA branch point/3' splice site region.

1988 • 23 citations

Revving up gene expression

1989 • 23 citations

Correct in vivo splicing of the mouse immunoglobulin kappa light-chain pre-mRNA is dependent on 5' splice-site position even in the absence of transcription.

1988 • 23 citations

Catalytic RNA and RNA Splicing

1989 • 15 citations

Co-localization of non-histone protein BA with U-snRNPs to the same regions of the cell nucleus

1985 • 12 citations

A yeast tRNA precursor containing a pre-mRNA intron is spliced via the pre-mRNA splicing mechanism.

1990 • 10 citations