A U1 snRNA:pre-mRNA base pairing interaction is required early in yeast spliceosome assembly but does not uniquely define the 5′ cleavage site.

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

[12] One-step gene disruption in yeast

1983 • 3,302 citations

A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

1984 • 2,401 citations

High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.

1979 • 1,504 citations

A compensatory base change in U1 snRNA suppresses a 5′ splice site mutation

1986 • 755 citations

PRE-mRNA SPLICING

1986 • 673 citations

The role of small nuclear ribonucleoprotein particles in pre-mRNA splicing

1987 • 594 citations

Inhibition of restriction endonuclease Nel I cleavage by phosphorothioate groups and its application to oligonucleotide-directed mutagenesis

1986 • 560 citations

Stepwise assembly of a pre-mRNA splicing complex requires U-snRNPs and specific intron sequences

1985 • 524 citations

Recognition of the TACTAAC box during mRNA splicing in yeast involves base pairing to the U2-like snRNA

1987 • 508 citations

Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs

1986 • 490 citations

The generality of self-splicing RNA: Relationship to nuclear mRNA splicing

1986 • 464 citations

Spliceosome assembly in yeast.

1987 • 402 citations

A single-base change at a splice site in a β0-thalassemic gene causes abnormal RNA splicing

1982 • 372 citations

Yeast mRNA splicing in vitro.

1985 • 337 citations

Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes

1986 • 283 citations

An ordered pathway of snRNP binding during mammalian pre-mRNA splicing complex assembly.

1987 • 272 citations

Affinity Chromatography of Splicing Complexes: U2, U5, and U4 + U6 Small Nuclear Ribonucleoprotein Particles in the Spliceosome

1986 • 238 citations

5′ cleavage site in eukaryotic pre-mRNA splicing is determined by the overall 5′ splice region, not by the conserved 5′ GU

1987 • 206 citations

mRNA splicing efficiency in yeast and the contribution of nonconserved sequences

1985 • 194 citations

RPC40, a unique gene for a subunit shared between yeast RNA polymerases A and C

1987 • 184 citations

Gel electrophoretic isolation of splicing complexes containing U1 small nuclear ribonucleoprotein particles.

1988 • 183 citations

Specific and stable intron-factor interactions are established early during in vitro pre-mRNA splicing

1985 • 180 citations

Mutations in a yeast intron demonstrate the importance of specific conserved nucleotides for the two stages of nuclear mRNA splicing

1986 • 150 citations

S. cerevisiae U1 RNA is large and has limited primary sequence homology to metazoan U1 snRNA

1987 • 131 citations

A point mutation in the conserved hexanucleotide at a yeast 5′ splice junction uncouples recognition, cleavage, and ligation

1985 • 129 citations

Saccharomyces cerevisiae Has a U1-Like Small Nuclear RNA with Unexpected Properties

1987 • 122 citations

A quantitative analysis of the effects of 5′ junction and TACTAAC box mutants and mutant combinations on yeast mRNA splicing

1985 • 115 citations

Small nuclear RNAs from Saccharomyces cerevisiae: unexpected diversity in abundance, size, and molecular complexity.

1986 • 105 citations

Gel Electrophoretic Isolation of Splicing Complexes Containing U1 Small Nuclear Ribonucleoprotein Particles

1988 • 95 citations

U1 small nuclear ribonucleoproteins are required early during spliceosome assembly.

1987 • 80 citations

A novel role for the 3' region of introns in pre-mRNA splicing of Saccharomyces cerevisiae.

1987 • 62 citations

Analysis of RNase-A-resistant regions of adenovirus 2 major late precursor-mRNA in splicing extracts reveals an ordered interaction of nuclear components with the substrate RNA

1987 • 53 citations

A chemical modification/interference study of yeast pre-mRNA spliceosome assembly and splicing.

1988 • 39 citations

U1 Small Nuclear Ribonucleoproteins are Required Early During Spliceosome Assembly

1987 • 35 citations

In vitrosplicing of mRNA precursors: 5' cleavage site can be predicted from the interaction between the 5' splice region and the 5' terminus of U1 snRNA

1988 • 32 citations