5' splice site selection in yeast: genetic alterations in base-pairing with U1 reveal additional requirements.

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

1987 • 5,413 citations

A catalogue of splice junction sequences

1982 • 3,861 citations

[12] One-step gene disruption in yeast

1983 • 3,302 citations

Are snRNPs involved in splicing?

1980 • 1,206 citations

A compensatory base change in U1 snRNA suppresses a 5′ splice site mutation

1986 • 755 citations

The U1 small nuclear RNA-protein complex selectively binds a 5′ splice site in vitro

1983 • 593 citations

U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing

1985 • 568 citations

A mechanism for RNA splicing.

1980 • 553 citations

Multiple factors including the small nuclear ribonucleoproteins U1 and U2 are necessary for Pre-mRNA splicing in vitro

1985 • 442 citations

Spliceosomal RNA U6 is remarkably conserved from yeast to mammals

1988 • 428 citations

The 5′ terminus of the RNA moiety of U1 small nuclear ribonucleoprotein particles is required for the splicing of messenger RNA precursors

1984 • 404 citations

Sequence requirements for splicing of higher eukaryotic nuclear pre-mRNA

1986 • 389 citations

Lariat structures are in vivo intermediates in yeast pre-mRNA splicing

1984 • 379 citations

The 3′ Splice Site of Pre-Messenger RNA Is Recognized by a Small Nuclear Ribonucleoprotein

1985 • 310 citations

A comparison of yeast ribosomal protein gene DNA sequences

1984 • 256 citations

Evidence for the biochemical role of an internal sequence in yeast nuclear mRNA introns: Implications for U1 RNA and metazoan mRNA splicing

1983 • 239 citations

Mutations in conserved intron sequences affect multiple steps in the yeast splicing pathway, particularly assembly of the spliceosome.

1986 • 215 citations

5′ cleavage site in eukaryotic pre-mRNA splicing is determined by the overall 5′ splice region, not by the conserved 5′ GU

1987 • 206 citations

Molecular consequences of specific intron mutations on yeast mRNA splicing in vivo and in vitro

1985 • 172 citations

An essential yeast snRNA with a U5-like domain is required for splicing in vivo

1987 • 168 citations

Splice site selection and ribonucleoprotein complex assembly during in vitro pre-mRNA splicing.

1988 • 163 citations

U2 RNA from yeast is unexpectedly large and contains homology to vertebrate U4, U5, and U6 small nuclear RNAs

1986 • 160 citations

Mutations in a yeast intron demonstrate the importance of specific conserved nucleotides for the two stages of nuclear mRNA splicing

1986 • 150 citations

Yeast contains small nuclear RNAs encoded by single copy genes

1983 • 133 citations

S. cerevisiae U1 RNA is large and has limited primary sequence homology to metazoan U1 snRNA

1987 • 131 citations

A point mutation in the conserved hexanucleotide at a yeast 5′ splice junction uncouples recognition, cleavage, and ligation

1985 • 129 citations

Translationsl regulation of expression of the bacteriophage T4 lysozyme gene

1986 • 122 citations

Saccharomyces cerevisiae Has a U1-Like Small Nuclear RNA with Unexpected Properties

1987 • 122 citations

A quantitative analysis of the effects of 5′ junction and TACTAAC box mutants and mutant combinations on yeast mRNA splicing

1985 • 115 citations

Autogenous regulation of splicing of the transcript of a yeast ribosomal protein gene.

1986 • 111 citations

An essential snRNA from S. cerevisiae has properties predicted for U4, including interaction with a U6-like snRNA

1987 • 104 citations

A trans-acting suppressor restores splicing of a yeast intron with a branch point mutation.

1987 • 95 citations

The yeast ribosomal protein L32 and its gene.

1987 • 92 citations

The natural 5' splice site of simian virus 40 large T antigen can be improved by increasing the base complementarity to U1 RNA.

1987 • 84 citations

Construction of a yeast actin gene intron deletion mutant that is defective in splicing and leads to the accumulation of precursor RNA in transformed yeast cells.

1982 • 58 citations

In vitrosplicing of mRNA precursors: 5' cleavage site can be predicted from the interaction between the 5' splice region and the 5' terminus of U1 snRNA

1988 • 32 citations

Development of an Efficient Method for Generating and Screening Active Trypsin and Trypsin Variants

1988 • 30 citations