Identification and characterization of three members of the human SR family of pre-mRNA splicing factors.

A general method applicable to the search for similarities in the amino acid sequence of two proteins

1970 • 11,049 citations

[6] Use of T7 RNA polymerase to direct expression of cloned genes

1990 • 6,681 citations

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1984 • 6,459 citations

SR proteins: a conserved family of pre-mRNA splicing factors.

1992 • 766 citations

Specific interactions between proteins implicated in splice site selection and regulated alternative splicing

1993 • 741 citations

RNA recognition: towards identifying determinants of specificity

1991 • 739 citations

Regulation of alternative pre-mRNA splicing by hnRNP A1 and splicing factor SF2

1992 • 724 citations

Differential transcriptional activation by Oct-1 and Oct-2: Interdependent activation domains induce Oct-2 phosphorylation

1990 • 696 citations

RNA-binding proteins as developmental regulators.

1989 • 679 citations

Normal and mutant human β-globin pre-mRNAs are faithfully and efficiently spliced in vitro

1984 • 679 citations

Analysis of the RNA-recognition motif and RS and RGG domains: conservation in metazoan pre-mRNA splicing factors

1993 • 663 citations

Specific transcription and RNA splicing defects in five cloned β-thalassaemia genes

1983 • 627 citations

Protein–protein interactions and 5'-splice-site recognition in mammalian mRNA precursors

1994 • 622 citations

Regulation of Alternative Splicing in Vivo by Overexpression of Antagonistic Splicing Factors

1994 • 612 citations

A serine kinase regulates intracellular localization of splicing factors in the cell cycle

1994 • 511 citations

Functional expression of cloned human splicing factor SF2: homology to rna-binding proteins, U1 70K, and drosophila splicing regulators

1991 • 508 citations

Distinct Functions of SR Proteins in Alternative pre-mRNA Splicing

1993 • 407 citations

Purification and characterization of pre-mRNA splicing factor SF2 from HeLa cells.

1990 • 359 citations

Primary structure of the human splicing factor asf reveals similarities with drosophila regulators

1991 • 357 citations

A conserved family of nuclear phosphoproteins localized to sites of polymerase II transcription.

1991 • 344 citations

A splicing enhancer complex controls alternative splicing of doublesex pre-mRNA

1993 • 332 citations

Function of conserved domains of hnRNP A1 and other hnRNP A/B proteins.

1994 • 321 citations

Normal human tissues, in addition to some tumors, express multiple different CD44 isoforms.

1994 • 311 citations

A splicing enhancer in the human fibronectin alternate ED1 exon interacts with SR proteins and stimulates U2 snRNP binding.

1993 • 300 citations

General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer.

1993 • 286 citations

In vivo evidence that transcription and splicing are coordinated by a recruiting mechanism

1993 • 272 citations

Specific commitment of different pre-mRNAs to splicing by single SR proteins

1993 • 272 citations

SR proteins promote the first specific recognition of Pre-mRNA and are present together with the U1 small nuclear ribonucleoprotein particle in a general splicing enhancer complex.

1994 • 267 citations

Isolation of a Complementary DNA that Encodes the Mammalian Splicing Factor SC35

1992 • 244 citations

Functional analysis of pre-mRNA splicing factor SF2/ASF structural domains.

1993 • 243 citations

General splicing factors SF2 and SC35 have equivalent activities in vitro, and both affect alternative 5' and 3' splice site selection.

1992 • 241 citations

Modulation of exon skipping and inclusion by heterogeneous nuclear ribonucleoprotein A1 and pre-mRNA splicing factor SF2/ASF.

1993 • 241 citations

The 35-kDa mammalian splicing factor SC35 mediates specific interactions between U1 and U2 small nuclear ribonucleoprotein particles at the 3' splice site.

1992 • 198 citations

Pathways for selection of 5′ splice sites by U1 snRNPs and SF2/ASF.

1993 • 192 citations

Functional domains of the human splicing factor ASF/SF2.

1993 • 188 citations

Adenovirus E1A coding sequences that enable ras and pmt oncogenes to transform cultured primary cells.

1986 • 161 citations

Characterization and cloning of the human splicing factor 9G8: a novel 35 kDa factor of the serine/arginine protein family.

1994 • 155 citations

Differential splicing yields novel adenovirus 5 E1A mRNAs that encode 30 kd and 35 kd proteins.

1987 • 142 citations

Two members of a conserved family of nuclear phosphoproteins are involved in pre-mRNA splicing.

1992 • 131 citations

A mutant nuclear protein with similarity to RNA binding proteins interferes with nuclear import in yeast.

1992 • 119 citations

The SR protein B52/SRp55 is essential for Drosophila development.

1994 • 112 citations

The human splicing factor ASF/SF2 can specifically recognize pre-mRNA 5' splice sites.

1994 • 107 citations

Human SR Proteins and Isolation of a cDNA Encoding SRp75

1993 • 105 citations

Characterization of a Drosophila protein associated with boundaries of transcriptionally active chromatin.

1991 • 104 citations

Novel delayed-early and highly insulin-induced growth response genes. Identification of HRS, a potential regulator of alternative pre-mRNA splicing

1993 • 104 citations

The Drosophila RNA-binding protein RBP1 is localized to transcriptionally active sites of chromosomes and shows a functional similarity to human splicing factor ASF/SF2.

1992 • 90 citations

A potential splicing factor is encoded by the opposite strand of the trans-spliced c-myb exon.

1992 • 89 citations

Autoregulation and multifunctionality among trans-acting factors that regulate alternative pre-mRNA processing.

1992 • 80 citations

A differentially expressed murine RNA encoding a protein with similarities to two types of nucleic acid binding motifs

1991 • 77 citations

Modulation of Exon Skipping and Inclusion by Heterogeneous Nuclear Ribonucleoprotein A1 and Pre-mRNA Splicing Factor SF2/ASF

1993 • 76 citations

Adenovirus E1A Coding Sequences That Enable ras and pmt Oncogenes To Transform Cultured Primary Cells

1986 • 72 citations

Non-snRNP protein splicing factors

1993 • 71 citations

Transitions from high to low molecular weight isoforms of CD45 (T200) involve rapid activation of alternate mRNA splicing and slow turnover of surface CD45R.

1989 • 70 citations

The SR Protein B52/SRp55 Is Essential for Drosophila Development

1994 • 51 citations

Protein phosphatase 1 can modulate alternative 5′ splice site selection in a HeLa splicing extract

1994 • 47 citations

Several mRNAs with variable 3' untranslated regions and different stability encode the human PR264/SC35 splicing factor.

1994 • 34 citations

Multiple activities of the human splicing factor ASF.

1992 • 23 citations

ASF alternative transcripts are highly conserved between mouse and man

1992 • 12 citations