Pathways for selection of 5′ splice sites by U1 snRNPs and SF2/ASF.

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

1988 • 17,059 citations

Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei

1983 • 11,902 citations

A catalogue of splice junction sequences

1982 • 3,861 citations

RNA splice junctions of different classes of eukaryotes: sequence statistics and functional implications in gene expression

1987 • 2,333 citations

SR proteins: a conserved family of pre-mRNA splicing factors.

1992 • 766 citations

A compensatory base change in U1 snRNA suppresses a 5′ splice site mutation

1986 • 755 citations

Exon definition may facilitate splice site selection in RNAs with multiple exons.

1990 • 726 citations

Regulation of alternative pre-mRNA splicing by hnRNP A1 and splicing factor SF2

1992 • 724 citations

Normal and mutant human β-globin pre-mRNAs are faithfully and efficiently spliced in vitro

1984 • 679 citations

The U1 small nuclear RNA-protein complex selectively binds a 5′ splice site in vitro

1983 • 593 citations

A survey on intron and exon lengths

1988 • 569 citations

The Origin and Evolution of Retroposons

1985 • 555 citations

Associations between distinct pre-mRNA splicing components and the cell nucleus.

1991 • 523 citations

Functional expression of cloned human splicing factor SF2: homology to rna-binding proteins, U1 70K, and drosophila splicing regulators

1991 • 508 citations

A protein factor, ASF, controls cell-specific alternative splicing of SV40 early pre-mRNA in vitro

1990 • 500 citations

A role for exon sequences and splice-site proximity in splice-site selection

1986 • 494 citations

The essential pre-mRNA splicing factor SF2 influences 5′ splice site selection by activating proximal sites

1990 • 454 citations

Multiple factors including the small nuclear ribonucleoproteins U1 and U2 are necessary for Pre-mRNA splicing in vitro

1985 • 442 citations

Identification of functional U1 snRNA-pre-mRNA complexes committed to spliceosome assembly and splicing

1989 • 391 citations

Interactions of small nuclear RNA's with precursor messenger RNA during in vitro splicing

1992 • 367 citations

A U1 snRNA:pre-mRNA base pairing interaction is required early in yeast spliceosome assembly but does not uniquely define the 5′ cleavage site.

1988 • 364 citations

Purification and characterization of pre-mRNA splicing factor SF2 from HeLa cells.

1990 • 359 citations

Primary structure of the human splicing factor asf reveals similarities with drosophila regulators

1991 • 357 citations

5' splice site selection in yeast: genetic alterations in base-pairing with U1 reveal additional requirements.

1988 • 307 citations

An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway.

1991 • 297 citations

A protein that specifically recognizes the 3′ splice site of mammalian pre-mRNA introns is associated with a small nuclear ribonucleoprotein

1986 • 274 citations

Site-specific cross-linking of mammalian U5 snRNP to the 5' splice site before the first step of pre-mRNA splicing.

1992 • 273 citations

Exon Definition May Facilitate Splice Site Selection in RNAs with Multiple Exons

1990 • 269 citations

Comparison of Total Sequence of a Cloned Rabbit β-Globin Gene and Its Flanking Regions with a Homologous Mouse Sequence

1979 • 256 citations

Biochemical characterization of U2 snRNP auxiliary factor: an essential pre-mRNA splicing factor with a novel intranuclear distribution.

1991 • 255 citations

Signals for the selection of a splice site in pre-mRNA

1987 • 247 citations

Mammalian nuclei contain foci which are highly enriched in components of the pre-mRNA splicing machinery.

1991 • 243 citations

Modulation of exon skipping and inclusion by heterogeneous nuclear ribonucleoprotein A1 and pre-mRNA splicing factor SF2/ASF.

1993 • 241 citations

General splicing factors SF2 and SC35 have equivalent activities in vitro, and both affect alternative 5' and 3' splice site selection.

1992 • 241 citations

Pre-mRNA splicing in vitro requires intact U4/U6 small nuclear ribonucleoprotein

1986 • 222 citations

Protein components specifically associated with prespliceosome and spliceosome complexes.

1992 • 218 citations

The 35-kDa mammalian splicing factor SC35 mediates specific interactions between U1 and U2 small nuclear ribonucleoprotein particles at the 3' splice site.

1992 • 198 citations

Small nuclear ribonucleoproteins and heterogeneous nuclear ribonucleoproteins in the amphibian germinal vesicle: loops, spheres, and snurposomes.

1991 • 182 citations

Specific and stable intron-factor interactions are established early during in vitro pre-mRNA splicing

1985 • 180 citations

Activation of c-src neuron-specific splicing by an unusual RNA element in vivo and in vitro

1992 • 171 citations

Splice site selection and ribonucleoprotein complex assembly during in vitro pre-mRNA splicing.

1988 • 163 citations

The role of nucleotide sequences in splice site selection in eukaryotic pre-messenger RNA

1986 • 148 citations

Do exons code for structural or functional units in proteins?

1988 • 147 citations

Multiple interactions between the splicing substrate and small nuclear ribonucleoproteins in spliceosomes.

1987 • 143 citations

U1 and U2 small nuclear RNAs are present in nuclear speckles.

1992 • 141 citations

Localization of a base-paired interaction between small nuclear RNAs U4 and U6 in intact U4/U6 ribonucleoprotein particles by psoralen cross-linking

1985 • 137 citations

Two members of a conserved family of nuclear phosphoproteins are involved in pre-mRNA splicing.

1992 • 131 citations

Factors influencing alternative splice site utilization in vivo.

1987 • 120 citations

The 5′ splice site: phylogetic evalution and variable geometry of association with U1RNA

1989 • 113 citations

Mechanism for cryptic splice site activation during pre-mRNA splicing.

1990 • 108 citations

Splicing of SV40 early pre-mRNA to large T and small t mRNAs utilizes different patterns of lariat branch sites

1987 • 93 citations

Hierarchy for 5′ splice site preference determined in vivo

1990 • 87 citations

RNA processing : a practical approach

1994 • 86 citations

Multiple Interactions between the Splicing Substrate and Small Nuclear Ribonucleoproteins in Spliceosomes

1987 • 86 citations

The natural 5' splice site of simian virus 40 large T antigen can be improved by increasing the base complementarity to U1 RNA.

1987 • 84 citations

Alternative splicing of E1A transcripts of adenovirus requires appropriate ionic conditions in vitro

1987 • 77 citations

Modulation of Exon Skipping and Inclusion by Heterogeneous Nuclear Ribonucleoprotein A1 and Pre-mRNA Splicing Factor SF2/ASF

1993 • 76 citations

Interaction of hnRNP A1 with snRNPs and pre-mRNAs: evidence for a possible role of A1 RNA annealing activity in the first steps of spliceosome assembly

1992 • 49 citations

Factors Influencing Alternative Splice Site Utilization In Vivo

1987 • 48 citations

Short donor site sequences inserted within the intron of beta-globin pre-mRNA serve for splicing in vitro.

1988 • 36 citations

Recognition of Mutant and Cryptic 5′ Splice Sites by the U1 Small Nuclear Ribonucleoprotein In Vitro

1987 • 35 citations

Influences of separation and adjacent sequences on the use of alternative 5′ splice sites

1991 • 32 citations

The Natural 5′ Splice Site of Simian Virus 40 Large T Antigen can be improved by increasing the base Complementarity to U1 RNA

1987 • 29 citations

Recognition of 5' and 3' splice site sequences in pre-mRNA studied with a filter binding technique.

1987 • 26 citations

Differential nuclease sensitivity identifies tight contacts between yeast pre-mRNA and spliceosomes.

1986 • 24 citations

Correct in vivo splicing of the mouse immunoglobulin kappa light-chain pre-mRNA is dependent on 5' splice-site position even in the absence of transcription.

1988 • 23 citations

Accurate 5' splice-site selection in mouse kappa immunoglobulin light chain premessenger RNAs is not cell-type-specific.

1987 • 22 citations

Oligonudeotide-targeted degradation of U1 and U2 snRNAs reveals differential interactions of simian virus 40 pre-mRNAs with snRNPs

1989 • 18 citations

Comparison of in vitro and in vivo splice site selection in kappa-immunoglobulin precursor mRNA.

1988 • 17 citations

Short Donor Site Sequences Inserted within the Intron of β-Globin Pre-mRNA Serve for Splicing In Vitro

1988 • 16 citations

In vitro splicing of kappa immunoglobulin precursor mRNA.

1987 • 13 citations

In Vitro Splicing of Kappa Immunoglobulin Precursor mRNA

1987 • 10 citations

Comparison of in Vitro and in Vivo Splice Site Selection in κ-Immunoglobulin Precursor mRNA

1988 • 6 citations