Vacuole Biogenesis in Saccharomyces cerevisiae : Protein Transport Pathways to the Yeast Vacuole

SNAP receptors implicated in vesicle targeting and fusion

1993 • 3,220 citations

Mechanisms of intracellular protein transport

1994 • 2,280 citations

The ubiquitin-proteasome proteolytic pathway

1994 • 1,778 citations

Isolation and characterization of autophagy‐defective mutants of Saccharomyces cerevisiae

1993 • 1,649 citations

ENDOCYTOSIS AND MOLECULAR SORTING

1996 • 1,537 citations

A new vital stain for visualizing vacuolar membrane dynamics and endocytosis in yeast.

1995 • 1,352 citations

The small GTPase rab5 functions as a regulatory factor in the early endocytic pathway

1992 • 1,342 citations

Autophagy in yeast demonstrated with proteinase-deficient mutants and conditions for its induction.

1992 • 1,187 citations

Localization of low molecular weight GTP binding proteins to exocytic and endocytic compartments

1990 • 1,150 citations

rab5 controls early endosome fusion in vitro

1991 • 1,090 citations

STRUCTURE AND FUNCTION OF THE MANNOSE 6-PHOSPHATE/INSULINLIKE GROWTH FACTOR II RECEPTORS

1992 • 1,056 citations

A Receptor for the Selective Uptake and Degradation of Proteins by Lysosomes

1996 • 904 citations

Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants.

1992 • 832 citations

CLATHRIN-COATED VESICLE FORMATION AND PROTEIN SORTING: An Integrated Process

1997 • 793 citations

Ubiquitination of a Yeast Plasma Membrane Receptor Signals Its Ligand-Stimulated Endocytosis

1996 • 789 citations

The fungal vacuole: composition, function, and biogenesis

1990 • 758 citations

Distinct sets of SEC genes govern transport vesicle formation and fusion early in the secretory pathway

1990 • 721 citations

Clathrin, Adaptors, and Sorting

1990 • 704 citations

Peptide sequences that target cytosolic proteins for lysosomal proteolysis

1990 • 674 citations

Vesicle fusion from yeast to man

1994 • 635 citations

A regulatory hierarchy for cell specialization in yeast

1989 • 612 citations

Early stages in the yeast secretory pathway are required for transport of carboxypeptidase Y to the vacuole

1982 • 579 citations

Isolation of autophagocytosis mutants of Saccharomyces cerevisiae

1994 • 544 citations

Multivesicular endosomes containing internalized EGF-EGF receptor complexes mature and then fuse directly with lysosomes.

1996 • 531 citations

Autophagy and related mechanisms of lysosome-mediated protein degradation

1994 • 506 citations

The sorting receptor for yeast vacuolar carboxypeptidase Y is encoded by the VPS10 gene

1994 • 500 citations

Isolation and characterization of yeast mutants in the cytoplasm to vacuole protein targeting pathway.

1995 • 493 citations

Ultrastructural analysis of the autophagic process in yeast: detection of autophagosomes and their characterization

1994 • 480 citations

The Saccharomyces cerevisiae MYO2 gene encodes an essential myosin for vectorial transport of vesicles.

1991 • 471 citations

Apg1p, a novel protein kinase required for the autophagic process in Saccharomyces cerevisiae

1997 • 466 citations

Cofilin promotes rapid actin filament turnover in vivo

1997 • 466 citations

PROTEINASE MUTANTS OF SACCHAROMYCES CEREVISIAE

1977 • 460 citations

Homotypic vacuolar fusion mediated by t- and v-SNAREs

1997 • 457 citations

Actin microfilaments play a critical role in endocytosis at the apical but not the basolateral surface of polarized epithelial cells.

1993 • 438 citations

The AP-3 Adaptor Complex Is Essential for Cargo-Selective Transport to the Yeast Vacuole

1997 • 430 citations

Protein sorting in yeast: Mutants defective in vacuole biogenesis mislocalize vacuolar proteins into the late secretory pathway

1986 • 429 citations

Compartmental organization of Golgi-specific protein modification and vacuolar protein sorting events defined in a yeast sec18 (NSF) mutant.

1991 • 415 citations

Endosomal transport function in yeast requires a novel AAA-type ATPase, Vps4p

1997 • 412 citations

Organelle assembly in yeast: characterization of yeast mutants defective in vacuolar biogenesis and protein sorting.

1988 • 409 citations

PEP4 gene of Saccharomyces cerevisiae encodes proteinase A, a vacuolar enzyme required for processing of vacuolar precursors.

1986 • 400 citations

Isolation of yeast mutants defective in protein targeting to the vacuole.

1986 • 394 citations

VPS27 controls vacuolar and endocytic traffic through a prevacuolar compartment in Saccharomyces cerevisiae.

1995 • 393 citations

Rab1b regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments.

1991 • 391 citations

AP-3: an adaptor-like protein complex with ubiquitous expression

1997 • 389 citations

end3 and end4: two mutants defective in receptor-mediated and fluid-phase endocytosis in Saccharomyces cerevisiae.

1993 • 380 citations

Rab GTPases in vesicular transport

1993 • 379 citations

Aminopeptidase I of Saccharomyces cerevisiae is localized to the vacuole independent of the secretory pathway.

1992 • 371 citations

Ultrastructure of the yeast actin cytoskeleton and its association with the plasma membrane.

1994 • 370 citations

Characterization of the Adaptor-related Protein Complex, AP-3

1997 • 369 citations

A Multispecificity Syntaxin Homologue, Vam3p, Essential for Autophagic and Biosynthetic Protein Transport to the Vacuole

1997 • 349 citations

Actin and fimbrin are required for the internalization step of endocytosis in yeast.

1993 • 346 citations

Intracellular routing of transferrin and transferrin receptors in epidermoid carcinoma A431 cells

1983 • 330 citations

Protein sorting in yeast: The localization determinant of yeast vacuolar carboxypeptidase Y resides in the propeptide

1987 • 329 citations

Isolation of peroxisome-deficient mutants of Saccharomyces cerevisiae.

1989 • 326 citations

The role of Myo2, a yeast class V myosin, in vesicular transport.

1995 • 324 citations

The ABC-transporter Ste6 accumulates in the plasma membrane in a ubiquitinated form in endocytosis mutants.

1994 • 319 citations

Ubiquitination Mediated by the Npi1p/Rsp5p Ubiquitin-protein Ligase Is Required for Endocytosis of the Yeast Uracil Permease

1996 • 316 citations

Distinct sequence determinants direct intracellular sorting and modification of a yeast vacuolar protease

1987 • 312 citations

SED5 encodes a 39-kD integral membrane protein required for vesicular transport between the ER and the Golgi complex.

1992 • 311 citations

The ubiquitin conjugation system is required for ligand-induced endocytosis and degradation of the growth hormone receptor.

1996 • 307 citations

end5, end6, and end7: mutations that cause actin delocalization and block the internalization step of endocytosis in Saccharomyces cerevisiae.

1995 • 305 citations

A Novel RING Finger Protein Complex Essential for a Late Step in Protein Transport to the Yeast Vacuole

1997 • 304 citations

Mother Cell–Specific HO Expression in Budding Yeast Depends on the Unconventional Myosin Myo4p and Other Cytoplasmic Proteins

1996 • 299 citations

The PEP4 Gene Encodes an Aspartyl Protease Implicated in the Posttranslational Regulation of Saccharomyces cerevisiae Vacuolar Hydrolases

1986 • 292 citations

Synthetic-lethal interactions identify two novel genes, SLA1 and SLA2, that control membrane cytoskeleton assembly in Saccharomyces cerevisiae

1993 • 289 citations

Novel syntaxin homologue, Pep12p, required for the sorting of lumenal hydrolases to the lysosome-like vacuole in yeast.

1996 • 284 citations

Vps10p cycles between the late-Golgi and prevacuolar compartments in its function as the sorting receptor for multiple yeast vacuolar hydrolases.

1996 • 283 citations

The emergence of clathrin-independent pinocytic pathways

1995 • 282 citations

Multilamellar endosome-like compartment accumulates in the yeast vps28 vacuolar protein sorting mutant.

1996 • 280 citations

Endocytosis in yeast: Several of the yeast secretory mutants are defective in endocytosis

1985 • 280 citations

COPI- and COPII-coated vesicles bud directly from the endoplasmic reticulum in yeast

1995 • 278 citations

MEMBRANE PARTITIONING DURING CELL DIVISION

1993 • 273 citations

Endocytosis and degradation of the yeast uracil permease under adverse conditions

1994 • 266 citations

The GTPase Ypt7p of Saccharomyces cerevisiae is required on both partner vacuoles for the homotypic fusion step of vacuole inheritance.

1995 • 265 citations

The END3 gene encodes a protein that is required for the internalization step of endocytosis and for actin cytoskeleton organization in yeast.

1994 • 260 citations

Cytoplasm-to-vacuole targeting and autophagy employ the same machinery to deliver proteins to the yeast vacuole.

1996 • 259 citations

Endocytosis is required for the growth of vacuolar H(+)-ATPase-defective yeast: identification of six new END genes.

1994 • 258 citations

Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae.

1989 • 258 citations

Parallel secretory pathways to the cell surface in yeast.

1995 • 257 citations

Genetic and Phenotypic Overlap between Autophagy and the Cytoplasm to Vacuole Protein Targeting Pathway

1996 • 257 citations

Vps34p required for yeast vacuolar protein sorting is a multiple specificity kinase that exhibits both protein kinase and phosphatidylinositol-specific PI 3-kinase activities.

1994 • 245 citations

Organelle Inheritance

1996 • 241 citations

A novel adaptor-related protein complex.

1996 • 241 citations

Cis- and trans-acting functions required for endocytosis of the yeast pheromone receptors

1993 • 239 citations

Docking of Yeast Vacuoles Is Catalyzed by the Ras-like GTPase Ypt7p after Symmetric Priming by Sec18p (NSF)

1997 • 239 citations

Disruption of the single tropomyosin gene in yeast results in the disappearance of actin cables from the cytoskeleton

1989 • 239 citations

Endocytosis in yeast: Evidence for the involvement of a small GTP-binding protein (Ypt7p)

1992 • 235 citations

Lysosomal (Vacuolar) Proteinases of Yeast Are Essential Catalysts for Protein Degradation, Differentiation, and Cell Survival

1989 • 230 citations

Multiple methods of visualizing the yeast vacuole permit evaluation of its morphology and inheritance during the cell cycle.

1987 • 224 citations

Gene dosage-dependent secretion of yeast vacuolar carboxypeptidase Y.

1986 • 217 citations

A novel fluorescence-activated cell sorter-based screen for yeast endocytosis mutants identifies a yeast homologue of mammalian eps15.

1996 • 217 citations

The Yeast v-SNARE Vti1p Mediates Two Vesicle Transport Pathways through Interactions with the t-SNAREs Sed5p and Pep12p

1997 • 216 citations

Novel Golgi to vacuole delivery pathway in yeast: identification of a sorting determinant and required transport component

1997 • 215 citations

Role of three rab5-like GTPases, Ypt51p, Ypt52p, and Ypt53p, in the endocytic and vacuolar protein sorting pathways of yeast.

1994 • 211 citations

Courtship in S. cerevisiae: Both cell types choose mating partners by responding to the strongest pheromone signal

1990 • 209 citations

Mutation of a tyrosine localization signal in the cytosolic tail of yeast Kex2 protease disrupts Golgi retention and results in default transport to the vacuole.

1992 • 209 citations

Actin and myosin function in directed vacuole movement during cell division in Saccharomyces cerevisiae.

1996 • 203 citations

The Ear of α-Adaptin Interacts with the COOH-terminal Domain of the Eps15 Protein

1996 • 186 citations

Receptor-Mediated Protein Sorting to the Vacuole in Yeast: Roles for a Protein Kinase, a Lipid Kinase and GTP-Binding Proteins

1995 • 184 citations

The tyrosine kinase substrate eps15 is constitutively associated with the plasma membrane adaptor AP-2.

1995 • 183 citations

Golgi and vacuolar membrane proteins reach the vacuole in vps1 mutant yeast cells via the plasma membrane.

1995 • 183 citations

Purification and characterization of constitutive secretory vesicles from yeast.

1987 • 179 citations

Membrane protein sorting in the yeast secretory pathway: evidence that the vacuole may be the default compartment.

1992 • 179 citations

A pathway for targeting soluble misfolded proteins to the yeast vacuole.

1996 • 178 citations

Down regulation of the α-factor pheromone receptor in S. cerevisiae

1986 • 177 citations

Regulated import and degradation of a cytosolic protein in the yeast vacuole

1991 • 174 citations

Ligand-induced polyubiquitination of the platelet-derived growth factor beta-receptor.

1992 • 173 citations

Ubiquitination of the yeast a-factor receptor.

1996 • 172 citations

G-protein ligands inhibit in vitro reactions of vacuole inheritance.

1994 • 171 citations

Aminopeptidase I Is Targeted to the Vacuole by a Nonclassical Vesicular Mechanism

1997 • 168 citations

Two yeast mutants defective in endocytosis are defective in pheromone response

1986 • 164 citations

The cytoplasmic tail domain of the vacuolar protein sorting receptor Vps10p and a subset of VPS gene products regulate receptor stability, function, and localization.

1995 • 157 citations

A role for clathrin in the sorting of vacuolar proteins in the Golgi complex of yeast.

1992 • 157 citations

The Membrane Protein Alkaline Phosphatase Is Delivered to the Vacuole by a Route That Is Distinct from the VPS-dependent Pathway

1997 • 153 citations

The Ypt1 GTPase is essential for the first two steps of the yeast secretory pathway.

1995 • 153 citations

A novel Sec18p/NSF-dependent complex required for Golgi-to-endosome transport in yeast.

1997 • 153 citations

Yeast vacuolar proenzymes are sorted in the late Golgi complex and transported to the vacuole via a prevacuolar endosome-like compartment.

1993 • 152 citations

Clathrin facilitates the internalization of seven transmembrane segment receptors for mating pheromones in yeast.

1993 • 151 citations

Dynamin GTPase, a force‐generating molecular switch

1996 • 150 citations

Involvement of Ypt7p, a small GTPase, in traffic from late endosome to the vacuole in yeast

1993 • 150 citations

Mutations in the VPS45 gene, a SEC1 homologue, result in vacuolar protein sorting defects and accumulation of membrane vesicles

1994 • 150 citations

Selective and immediate effects of clathrin heavy chain mutations on Golgi membrane protein retention in Saccharomyces cerevisiae.

1992 • 147 citations

Yeast carboxypeptidase Y vacuolar targeting signal is defined by four propeptide amino acids.

1990 • 147 citations

Suppressors of YCK-encoded yeast casein kinase 1deficiency define the four subunits of a novel clathrin AP-like complex

1997 • 147 citations

Transport of a Large Oligomeric Protein by the Cytoplasm to Vacuole Protein Targeting Pathway

1997 • 146 citations

Direct evidence for ligand-induced internalization of the yeast alpha-factor pheromone receptor.

1994 • 142 citations

Localization of a yeast early Golgi mannosyltransferase, Och1p, involves retrograde transport.

1996 • 142 citations

β3A-adaptin, a Subunit of the Adaptor-like Complex AP-3

1997 • 134 citations

The Yeast Gene, MDM20, Is Necessary for Mitochondrial Inheritance and Organization of the Actin Cytoskeleton

1997 • 133 citations

Vac7p, a Novel Vacuolar Protein, Is Required for Normal Vacuole Inheritance and Morphology

1997 • 131 citations

The epidermal growth factor receptor is covalently linked to ubiquitin.

1995 • 130 citations

Identification of MYO4, a second class V myosin gene in yeast

1994 • 130 citations

EH Domain Proteins Pan1p and End3p Are Components of a Complex That Plays a Dual Role in Organization of the Cortical Actin Cytoskeleton and Endocytosis in Saccharomyces cerevisiae

1997 • 128 citations

Yeast Vps45p is a Sec1p-like protein required for the consumption of vacuole-targeted, post-Golgi transport vesicles.

1994 • 127 citations

Coats and vesicle budding

1997 • 127 citations

Identification of a novel sequence mediating regulated endocytosis of the G protein-coupled alpha-pheromone receptor in yeast.

1993 • 126 citations

A novel pathway of import of alpha-mannosidase, a marker enzyme of vacuolar membrane, in Saccharomyces cerevisiae.

1990 • 125 citations

The yeast multidrug transporter Pdr5 of the plasma membrane is ubiquitinated prior to endocytosis and degradation in the vacuole

1996 • 124 citations

Partial purification and characterization of early and late endosomes from yeast. Identification of four novel proteins

1993 • 120 citations

Catabolite Inactivation of Fructose-1,6-bisphosphatase of Saccharomyces cerevisiae

1995 • 116 citations

In vitro reactions of vacuole inheritance in Saccharomyces cerevisiae.

1992 • 113 citations

Molecular analysis of the yeast VPS3 gene and the role of its product in vacuolar protein sorting and vacuolar segregation during the cell cycle.

1990 • 112 citations

Detection of an intermediate compartment involved in transport of alpha-factor from the plasma membrane to the vacuole in yeast.

1990 • 111 citations

Selective Uptake of Cytosolic, Peroxisomal, and Plasma Membrane Proteins into the Yeast Lysosome for Degradation

1996 • 104 citations

Two Separate Signals Act Independently to Localize a Yeast Late Golgi Membrane Protein through a Combination of Retrieval and Retention

1997 • 104 citations

Cloning and heterologous expression of glycosidase genes from Saccharomyces cerevisiae.

1987 • 103 citations

Identification of a cytoplasm to vacuole targeting determinant in aminopeptidase I.

1996 • 102 citations

Vam3p, a new member of syntaxin related protein, is required for vacuolar assembly in the yeast Saccharomyces cerevisiae

1997 • 102 citations

Protein transport to the yeast vacuole

1995 • 102 citations

Metabolic instability and constitutive endocytosis of STE6, the a-factor transporter of Saccharomyces cerevisiae.

1994 • 102 citations

Nucleotide sequence ofAMS1, the structure gene of vacuolarα-mannosidase of Saccharomyces cerevisiae

1989 • 102 citations

Intervacuole Exchange in the Yeast Zygote: A New Pathway in Organelle Communication

1988 • 100 citations

The EH-Domain-Containing Protein Pan1 Is Required for Normal Organization of the Actin Cytoskeleton in Saccharomyces cerevisiae

1996 • 99 citations

Identification of Novel Vesicles in the Cytosol to Vacuole Protein Degradation Pathway

1997 • 97 citations

AUT1, a gene essential for autophagocytosis in the yeast Saccharomyces cerevisiae

1997 • 97 citations

Mutants of Saccharomyces cerevisiae that block intervacuole vesicular traffic and vacuole division and segregation.

1990 • 97 citations

Sorting of membrane proteins in the yeast secretory pathway.

1994 • 95 citations

Actin-, myosin- and ubiquitin-dependent endocytosis

1996 • 94 citations

PEP4 Gene of Saccharomyces cerevisiae Encodes Proteinase A, a Vacuolar Enzyme Required for Processing of Vacuolar Precursors

1986 • 94 citations

Molecular characterization of VAC1, a gene required for vacuole inheritance and vacuole protein sorting.

1992 • 94 citations

Multiple classes of yeast mutants are defective in vacuole partitioning yet target vacuole proteins correctly.

1996 • 92 citations

Catabolite inactivation of the yeast maltose transporter occurs in the vacuole after internalization by endocytosis

1995 • 92 citations

Clathrin, Adapters, And Sorting

1990 • 89 citations

Isolation of Degradation-Deficient Mutants Defective in the Targeting of Fructose-1,6-bisphosphatase into the Vacuole for Degradation in Saccharomyces cerevisiae

1996 • 88 citations

Tracing intracellular proteolytic pathways. Proteolysis of fatty acid synthase and other cytoplasmic proteins in the yeast Saccharomyces cerevisiae.

1993 • 87 citations

Cytosol- and clathrin-dependent stimulation of endocytosis in vitro by purified adaptors.

1992 • 84 citations

Calcium-independent calmodulin requirement for endocytosis in yeast.

1994 • 82 citations

Processing pathway for protease B of Saccharomyces cerevisiae.

1989 • 81 citations

Prelysosomal and lysosomal connections between autophagy and endocytosis

1992 • 81 citations

The linker region of the ABC-transporter Ste6 mediates ubiquitination and fast turnover of the protein

1997 • 79 citations

Regulation of Inositol Transport in Saccharomyces cerevisiae Involves Inositol-induced Changes in Permease Stability and Endocytic Degradation in the Vacuole

1995 • 78 citations

Catabolite inactivation of fructose‐1,6‐bisphosphatase in yeast is mediated by the proteasome

1994 • 77 citations

Determination of four biochemically distinct, sequential stages during vacuole inheritance in vitro.

1994 • 77 citations

Thioredoxin is required for vacuole inheritance in Saccharomyces cerevisiae.

1996 • 76 citations

Specific role for the PH domain of dynamin-1 in the regulation of rapid endocytosis in adrenal chromaffin cells

1997 • 75 citations

Half-life of the plasma membrane ATPase and its activating system in resting yeast cells

1991 • 74 citations

A Putative Zinc Finger Protein, Saccharomyces cerevisiae Vpsl8p, Affects Late Golgi Functions Required for Vacuolar Protein Sorting and Efficient α-Factor Prohormone Maturation

1991 • 74 citations

A Heterodimer of Thioredoxin and IB2 Cooperates with Sec18p (NSF) to Promote Yeast Vacuole Inheritance

1997 • 73 citations

Pheromones and pheromone receptors are the primary determinants of mating specificity in the yeast Saccharomyces cerevisiae.

1989 • 73 citations

Isolation and characterization of PEP3, a gene required for vacuolar biogenesis in Saccharomyces cerevisiae.

1991 • 71 citations

Yeast aminopeptidase I chemical composition and catalytic properties

1976 • 70 citations

Vacuolar biogenesis in yeast: Sorting out the sorting proteins

1995 • 69 citations

Molecular Cloning and Sequencing of Genomic DNA Encoding Aminopeptidase I from Saccharomyces cerevisiae

1989 • 68 citations

Multiple Pathways for Vacuolar Sorting of Yeast Proteinase A

1996 • 61 citations

Activation of the proteinase B precursor of the yeast Saccharomyces cerevisiae by autocatalysis and by an internal sequence.

1991 • 60 citations

Vma22p Is a Novel Endoplasmic Reticulum-associated Protein Required for Assembly of the Yeast Vacuolar H+-ATPase Complex

1995 • 60 citations

Direct Evidence for Ligand-Induced Internalization of the Yeast α-Factor Pheromone Receptor

1994 • 57 citations

Protein Transport from the Cytoplasm into the Vacuole

1997 • 56 citations

The Yeast Plasma Membrane Uracil Permease Is Stabilized against Stress-Induced Degradation by a Point Mutation in a Cyclin-like "Destruction Box"

1994 • 44 citations

Immunoisolation of Kex2p-containing organelles from yeast demonstrates colocalisation of three processing proteinases to a single Golgi compartment

1993 • 40 citations

vac2: a yeast mutant which distinguishes vacuole segregation from Golgi-to-vacuole protein targeting.

1991 • 40 citations

The END3 gene encodes a protein that is required for the internalization step of endocytosis and for actin cytoskeleton organization in yeast

1995 • 37 citations

Ubiquitin, a central component of selective cytoplasmic proteolysis, is linked to proteins residing at the locus of non‐selective proteolysis, the vacuole

1992 • 36 citations

Selective Degradation of Cytosolic Proteins by Lysosomes^a

1992 • 34 citations

A truncated form of the Pho80 cyclin redirects the Pho85 kinase to disrupt vacuole inheritance in S. cerevisiae.

1995 • 29 citations

Cholesterol-independent Targeting of Golgi Membrane Proteins in Insect Cells

1997 • 21 citations

Isolation and Characterization of PEP3, a Gene Required for Vacuolar Biogenesis in Saccharomyces cerevisiae

1991 • 21 citations

Site of catabolite inactivation

1994 • 18 citations

The role of ATP hydrolysis in the function of the chaperonin GroEL: dynamic complex formation with GroES

1995 • 12 citations

A truncated form of the Pho80 cyclin of Saccharomyces cerevisiae induces expression of a small cytosolic factor which inhibits vacuole inheritance

1996 • 9 citations

Deleted Work

1955 • 0 citations